Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,068,135 G→T 7.3% F140L (TTC→TTA)  yggD ← MtlR family putative transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,068,1350GT 7.3% 679.7 / 18.6 193F140L (TTC→TTA) yggDMtlR family putative transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (84/94);  new base T (8/7);  total (92/101)
Fisher's exact test for biased strand distribution p-value = 7.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

TCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGG  >  CP009273/3067988‑3068279
                                                                                                                                                   |                                                                                                                                                
tctcTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAAAGCGCCTCTGACCAGACGgag                                                                                                                                                  >  2:3047619/1‑148 (MQ=255)
 ctctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGgaga                                                                                                                                                 >  2:296237/1‑148 (MQ=255)
  tctTCGTTCTGGTACTGTGCCTGTAACTACACCCCACACACCCAAAAAACAAAATACACCGCGTTAATTCCTTTAAGGTGGTCGTTACACCCAGCCACACCCCCCAACCGCCCCCCCCCCTAAAACGCGCCTCTGACCAGACGGAGaa                                                                                                                                                <  2:1515236/148‑1 (MQ=255)
   ctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGaaa                                                                                                                                               >  2:784616/1‑148 (MQ=255)
   ctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGaaa                                                                                                                                               >  2:2834551/1‑148 (MQ=255)
    ttCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAAt                                                                                                                                              >  2:2368833/1‑148 (MQ=255)
     tCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATc                                                                                                                                             >  1:3259582/1‑148 (MQ=255)
          cTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGa                                                                                                                                        <  2:2054394/148‑1 (MQ=255)
               aCTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTc                                                                                                                                   <  1:2516549/148‑1 (MQ=255)
                cTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCa                                                                                                                                  >  1:2345105/1‑148 (MQ=255)
                 tgtgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCAt                                                                                                                                 >  1:117396/1‑148 (MQ=255)
                 tgtgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCAt                                                                                                                                 >  1:2604627/1‑148 (MQ=255)
                 tgtgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCAt                                                                                                                                 <  2:1227783/148‑1 (MQ=255)
                   tgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCGAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCg                                                                                                                               >  1:791343/1‑148 (MQ=255)
                    gCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGc                                                                                                                              <  1:1489176/148‑1 (MQ=255)
                    gCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGc                                                                                                                              <  1:1488584/148‑1 (MQ=255)
                    gCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGc                                                                                                                              >  1:402299/1‑148 (MQ=255)
                     ccTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCt                                                                                                                             <  1:2744770/148‑1 (MQ=255)
                     ccTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCt                                                                                                                             <  1:2743651/148‑1 (MQ=255)
                     ccTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCt                                                                                                                             <  2:2497871/148‑1 (MQ=255)
                      cTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCtt                                                                                                                            <  1:1518675/148‑1 (MQ=255)
                      cTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAAGTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCtt                                                                                                                            <  2:1765258/148‑1 (MQ=255)
                       tGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGTCCATTCCGCTAAAAAGCGCCACTGACCAGACGGAGAAATCAGCGAACATCATAGCttt                                                                                                                           >  1:1022217/1‑148 (MQ=255)
                       tGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCttt                                                                                                                           >  1:1022220/1‑148 (MQ=255)
                         tATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTa                                                                                                                         <  2:1358014/148‑1 (MQ=255)
                                  cccATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCAt                                                                                                                >  2:2915586/1‑148 (MQ=255)
                                       tCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGc                                                                                                           <  2:656696/148‑1 (MQ=255)
                                        cACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTTACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCa                                                                                                          <  2:2856822/148‑1 (MQ=255)
                                         aCCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCAt                                                                                                         <  1:1188425/148‑1 (MQ=255)
                                           cGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGTACAATACGCTAAAAACCGCCGCAGACCAGACGGAGAAATAAGCGAACATCATCGCTTTTAAAGCGTCATATAGccacg                                                                                                       >  1:578603/1‑144 (MQ=255)
                                             ttAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTTTCCATTCCGCTAAAAAGCGCCTCTGACCAGACGGAGAAATAAGCGAACTTCATCGCTTTTAAAGCGTCATAGTGCATCGCa                                                                                                     >  1:2031651/1‑148 (MQ=255)
                                                aTTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTTACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATAGCTTTTAAAGCGTCATAGAGCAACGCATTg                                                                                                  >  2:855544/1‑148 (MQ=255)
                                                 ttCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTTACCATTCCGCTAAAAAGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAAGCGTCATAGAGCATCGCATTGc                                                                                                 >  1:3164527/1‑148 (MQ=255)
                                                 ttCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGc                                                                                                 >  2:2736960/1‑148 (MQ=255)
                                                 ttCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGc                                                                                                 >  2:51237/1‑148 (MQ=255)
                                                 ttCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTACGCTAAAACGCGCCACTGACCAGAAGGAGAAATCAGCGAACTTAATCGCTTTTAAAGCGTCATAGAGCATCCCATTGc                                                                                                 >  1:2663182/1‑148 (MQ=255)
                                                  tCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTTTCCAATGCGCTAAAACTCGCCACAGACCAGACGGAGAAATCAGCGAACATAATAGCGTTTAACGCGTCAAAGAGCATAGCATTggg                                                                                                >  1:2010116/1‑146 (MQ=255)
                                                  tCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCg                                                                                                >  2:3229990/1‑148 (MQ=255)
                                                    aaTTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCggg                                                                                              <  2:727195/148‑1 (MQ=255)
                                                     aTTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGt                                                                                             <  2:2880714/148‑1 (MQ=255)
                                                        ttCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTAt                                                                                          <  2:443949/148‑1 (MQ=255)
                                                         tCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATg                                                                                         <  2:687598/148‑1 (MQ=255)
                                                                gTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGa                                                                                  >  2:2395617/1‑148 (MQ=255)
                                                                  tAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGTCCAATACGCGAAAAAGCGCCAAAGGCCAGACGGAGAAATAAGCCAAAATAATAGCTTTTAACGCGTAAAAGATCAACGCATTGCGGGTTATTCGATTTACg                                                                                >  1:3104992/1‑148 (MQ=255)
                                                                  tAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg                                                                                <  2:1124064/148‑1 (MQ=255)
                                                                    aTTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGtt                                                                              >  2:2381549/1‑148 (MQ=255)
                                                                    aTTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGtt                                                                              >  1:1089552/1‑148 (MQ=255)
                                                                     ttCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTg                                                                             <  2:1096715/148‑1 (MQ=255)
                                                                     ttCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTACGCTAAAACGCGCCTCTGACCACACGGAGAAATCAGCGAACTTCATCGCGTTTAAAGCGTCATATAGCATCGCCTTGCGGGTTATGCTATTGACGTTg                                                                             >  1:2238608/1‑148 (MQ=255)
                                                                     ttCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACAAATCCGCAAAAACGCGCCTCTGACCAGACGGAGAAAACAGCGAACTTCATAGCTTTTAAAGCGTCATAGAGCATCGCATTTCGGGTTAAGCTATTGACGTTg                                                                             >  1:1330517/1‑148 (MQ=255)
                                                                      tCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGc                                                                            <  1:2327630/148‑1 (MQ=255)
                                                                      tCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGc                                                                            >  2:2567665/1‑148 (MQ=255)
                                                                      tCCATTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGc                                                                            <  1:2053425/148‑1 (MQ=255)
                                                                       ccTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCt                                                                           >  1:2733219/1‑148 (MQ=255)
                                                                        cTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTg                                                                          <  2:2702569/148‑1 (MQ=255)
                                                                        cTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTg                                                                          <  2:2703333/148‑1 (MQ=255)
                                                                          ttAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATAAGCGAACATCATCGCTTTTAAAGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGAAGTTGCTgtt                                                                        >  1:650520/1‑146 (MQ=255)
                                                                               gtggtCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAAt                                                                   >  2:393566/1‑148 (MQ=255)
                                                                                 ggtCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATcc                                                                 <  1:1314390/148‑1 (MQ=255)
                                                                                  gtCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATccc                                                                <  2:3259582/148‑1 (MQ=255)
                                                                                      ttACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgt                                                            >  1:725034/1‑148 (MQ=255)
                                                                                       tACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtc                                                           <  2:2634738/148‑1 (MQ=255)
                                                                                           gCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGGTATGCTATTGACGTTGCTGATAAAATCCCAGgtcatgt                                                       >  1:1585104/1‑148 (MQ=255)
                                                                                           gCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGgtcatgt                                                       <  1:2744519/148‑1 (MQ=255)
                                                                                                cGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATAAGCGAACTTAATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATACCAGGTCATGTCATCg                                                  >  1:883999/1‑148 (MQ=255)
                                                                                                   cAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCa                                               >  2:2020659/1‑148 (MQ=255)
                                                                                                     gcgcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCaaa                                             >  1:2189707/1‑148 (MQ=255)
                                                                                                     gcgcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCaaa                                             <  2:818107/148‑1 (MQ=255)
                                                                                                      cgcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAAt                                            <  2:3001685/148‑1 (MQ=255)
                                                                                                      cgcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAAt                                            <  1:1634714/148‑1 (MQ=255)
                                                                                                      cgcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAAt                                            <  1:2351922/148‑1 (MQ=255)
                                                                                                      cgcCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAAt                                            <  1:1939643/148‑1 (MQ=255)
                                                                                                        ccGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATcc                                          <  1:1452814/148‑1 (MQ=255)
                                                                                                        ccGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATcc                                          <  1:2834551/148‑1 (MQ=255)
                                                                                                           ttttGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCggg                                       >  1:1532982/1‑148 (MQ=255)
                                                                                                            tttGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGc                                      >  1:3183754/1‑148 (MQ=255)
                                                                                                              tGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGt                                    <  2:2349357/148‑1 (MQ=255)
                                                                                                               gACCATTCCGCTACAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGtt                                   <  2:3104992/148‑1 (MQ=255)
                                                                                                                aCCAGTCCGCTAAAACGCGCCTCTGACCAGACGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGttt                                  <  2:2227149/148‑1 (MQ=255)
                                                                                                                 ccATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTc                                 <  1:311838/148‑1 (MQ=255)
                                                                                                                 ccATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTc                                 >  2:1487029/1‑148 (MQ=255)
                                                                                                                     tCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATcc                             <  1:2377465/148‑1 (MQ=255)
                                                                                                                      ccGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCt                            <  2:1439857/148‑1 (MQ=255)
                                                                                                                        gCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgt                          <  2:1532978/148‑1 (MQ=255)
                                                                                                                        gCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgt                          <  1:296237/148‑1 (MQ=255)
                                                                                                                         cTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCCAATCCGGGCGTGTCATCCTgtt                         >  1:2886498/1‑148 (MQ=255)
                                                                                                                              aCGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTgttcgttc                    <  2:402299/148‑1 (MQ=255)
                                                                                                                                gcgcCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAg                  <  2:1022220/148‑1 (MQ=255)
                                                                                                                                   ccTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTa               >  1:1784572/1‑148 (MQ=255)
                                                                                                                                     tctGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTTTTCGTTCAGGTact             >  2:1749310/1‑146 (MQ=255)
                                                                                                                                      ctGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATg            >  2:498000/1‑148 (MQ=255)
                                                                                                                                      ctGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATg            >  2:2161340/1‑148 (MQ=255)
                                                                                                                                      ctGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATg            <  2:791343/148‑1 (MQ=255)
                                                                                                                                            aGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGaa      <  2:1797251/148‑1 (MQ=255)
                                                                                                                                            aGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGaa      <  2:1296621/148‑1 (MQ=255)
                                                                                                                                              aCGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACt    >  2:195207/1‑148 (MQ=255)
                                                                                                                                              aCGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACt    <  1:2160596/148‑1 (MQ=255)
                                                                                                                                               cGGATAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTg   <  2:2483866/148‑1 (MQ=255)
                                                                                                                                                ggAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTgg  <  1:796604/148‑1 (MQ=255)
                                                                                                                                                ggAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTgg  <  1:481534/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTGCTGATAAAATCCCAGGTCATGTCATCGGCAAATCCGGGCGTTTCATCCTGTTCGTTCAGGTAATGCCAGAACTGG  >  CP009273/3067988‑3068279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑