Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,110,213 T→A 92.2% A753A (GCA→GCT)  yghJ ← DUF4092 family putative lipoprotein peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,110,2130TA92.2% 429.5 / 20.8 139A753A (GCA→GCT) yghJDUF4092 family putative lipoprotein peptidase
Reads supporting (aligned to +/- strand):  ref base T (6/5);  new base A (60/68);  total (66/73)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

CTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTC  >  CP009273/3110068‑3110357
                                                                                                                                                 |                                                                                                                                                
cTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGc                                                                                                                                                >  1:1494568/1‑148 (MQ=255)
 ttCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGcc                                                                                                                                               >  2:1199341/1‑148 (MQ=255)
    cccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATc                                                                                                                                            >  1:2407585/1‑148 (MQ=255)
     ccTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCg                                                                                                                                           <  2:2094391/148‑1 (MQ=255)
       tGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGAc                                                                                                                                         >  1:2658146/1‑148 (MQ=255)
       tGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGAc                                                                                                                                         >  1:962813/1‑148 (MQ=255)
       tGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGAc                                                                                                                                         <  2:1556352/148‑1 (MQ=255)
         tCTTACTATCTATTGTGTACGGCAGCTCACCAACTACGGCAGGATAACGTTCATATAACCAAATGACAGTTGCGCGCTGCTGACGAACGAGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATAGACAg                                                                                                                                       >  2:1327502/1‑148 (MQ=255)
          cTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGa                                                                                                                                      >  1:1524293/1‑148 (MQ=255)
          cTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTAGTTCAGAGCCATCGACAGa                                                                                                                                      >  2:3279438/1‑148 (MQ=255)
           ttACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGAc                                                                                                                                     <  1:461565/148‑1 (MQ=255)
            tACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGAcc                                                                                                                                    >  2:2207223/1‑148 (MQ=255)
             aCTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCt                                                                                                                                   >  2:1530724/1‑148 (MQ=255)
              cTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTg                                                                                                                                  >  2:798266/1‑148 (MQ=255)
               tATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGc                                                                                                                                 >  1:3133533/1‑148 (MQ=255)
                      ggTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATcc                                                                                                                          >  2:2525242/1‑148 (MQ=255)
                           aCGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAg                                                                                                                     <  1:562898/148‑1 (MQ=255)
                            cGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGa                                                                                                                    <  1:17423/148‑1 (MQ=255)
                            cGGCAGCGCACCATCTACGGCAGGATAACGCTCATAGACCCAACTGCCCGTTGCGCGCGGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGa                                                                                                                    <  2:3054857/148‑1 (MQ=255)
                                aGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGca                                                                                                                <  1:2476041/148‑1 (MQ=255)
                                     aCCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACaaaa                                                                                                           <  1:3256402/148‑1 (MQ=255)
                                       cATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAcc                                                                                                         >  2:1105735/1‑148 (MQ=255)
                                       cATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAAcc                                                                                                         <  1:632787/148‑1 (MQ=255)
                                        aTCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCa                                                                                                        >  1:2123007/1‑148 (MQ=255)
                                          cTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGa                                                                                                      <  2:2148939/148‑1 (MQ=255)
                                              ggCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGAcgcg                                                                                                  >  1:583679/1‑148 (MQ=255)
                                                cAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGct                                                                                                <  2:104661/148‑1 (MQ=255)
                                                     tAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCtt                                                                                           <  2:696262/148‑1 (MQ=255)
                                                     tAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCtt                                                                                           <  2:696254/148‑1 (MQ=255)
                                                      aaCGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTc                                                                                          >  2:2850429/1‑148 (MQ=255)
                                                       aCGTTCATATACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTcc                                                                                         <  2:458299/148‑1 (MQ=255)
                                                       aCGTTCATATACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTcc                                                                                         <  2:457654/148‑1 (MQ=255)
                                                       aCGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTcc                                                                                         <  1:2715380/148‑1 (MQ=255)
                                                       aCGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTcc                                                                                         <  1:2657160/148‑1 (MQ=255)
                                                        cGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCt                                                                                        <  1:3098166/148‑1 (MQ=255)
                                                        cGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCt                                                                                        >  2:539389/1‑148 (MQ=255)
                                                          ttCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTa                                                                                      <  1:2514229/148‑1 (MQ=255)
                                                            cATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAg                                                                                    >  1:1772874/1‑148 (MQ=255)
                                                              tAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGAt                                                                                  >  1:1793942/1‑148 (MQ=255)
                                                                      aaTGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCtcatc                                                                          <  1:818192/148‑1 (MQ=255)
                                                                         gCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACg                                                                       <  2:2404185/148‑1 (MQ=255)
                                                                          cccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGt                                                                      >  2:3297838/1‑148 (MQ=255)
                                                                          cccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCAGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGt                                                                      <  2:289682/148‑1 (MQ=255)
                                                                          cccGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCAGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGt                                                                      <  2:289490/148‑1 (MQ=255)
                                                                            cGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGttt                                                                    <  2:2431659/148‑1 (MQ=255)
                                                                            cGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGttt                                                                    <  2:2433172/148‑1 (MQ=255)
                                                                             gTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGtttt                                                                   <  2:1807713/148‑1 (MQ=255)
                                                                             gTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCCTAAGATTGCTCATCACGtttt                                                                   >  1:3202257/1‑148 (MQ=255)
                                                                              ttGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTc                                                                  >  2:486131/1‑148 (MQ=255)
                                                                                gcgcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTcaa                                                                <  2:3212816/148‑3 (MQ=255)
                                                                                 cgcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCAt                                                               <  2:1377630/148‑1 (MQ=255)
                                                                                  gcgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATg                                                              <  2:1837319/148‑1 (MQ=255)
                                                                                   cgcTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGa                                                             >  2:1917168/1‑148 (MQ=255)
                                                                                     ctgctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATc                                                           <  1:2169705/148‑1 (MQ=255)
                                                                                     ctgctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATc                                                           <  2:3150112/148‑1 (MQ=255)
                                                                                      tgctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCa                                                          >  1:102342/1‑148 (MQ=255)
                                                                                        ctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGc                                                        <  2:1510120/148‑1 (MQ=255)
                                                                                        ctgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGc                                                        >  1:1721598/1‑148 (MQ=255)
                                                                                         tgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCa                                                       <  1:10089/148‑1 (MQ=255)
                                                                                         tgACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCa                                                       <  2:2288736/148‑1 (MQ=255)
                                                                                             gAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGa                                                   >  2:324590/1‑148 (MQ=255)
                                                                                                cgcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATcc                                                <  2:400424/148‑1 (MQ=255)
                                                                                                cgcgGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATcc                                                <  2:2203091/148‑1 (MQ=255)
                                                                                                    gTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCt                                            >  1:1270935/1‑148 (MQ=255)
                                                                                                    gTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCt                                            <  2:1151432/148‑1 (MQ=255)
                                                                                                     ttCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCtt                                           >  2:1470331/1‑148 (MQ=255)
                                                                                                       cGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTg                                         <  1:1105735/148‑1 (MQ=255)
                                                                                                        ggATACCCTTGCGGATCGTTATTTTCTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGt                                        <  1:849728/148‑1 (MQ=255)
                                                                                                         gATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                       >  2:2686269/1‑148 (MQ=255)
                                                                                                         gATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                       >  2:2686256/1‑148 (MQ=255)
                                                                                                         gATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                       >  2:2686268/1‑148 (MQ=255)
                                                                                                         gATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                       <  2:2658146/148‑1 (MQ=255)
                                                                                                         gATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGtt                                       >  2:2637718/1‑148 (MQ=255)
                                                                                                           tACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCa                                     <  2:631258/148‑1 (MQ=255)
                                                                                                               cTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATa                                 <  2:138386/148‑1 (MQ=255)
                                                                                                               cTTGCGGATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATa                                 >  1:2409946/1‑148 (MQ=255)
                                                                                                                    ggATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAACCCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGa                            <  2:2023451/148‑1 (MQ=255)
                                                                                                                    ggATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGa                            <  2:2021888/148‑1 (MQ=255)
                                                                                                                     gATCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGAt                           >  1:638765/1‑148 (MQ=255)
                                                                                                                      aTCGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATc                          <  2:459573/148‑1 (MQ=255)
                                                                                                                        cGTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAg                        >  1:980203/1‑148 (MQ=255)
                                                                                                                         gTTATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGa                       <  2:1139277/148‑1 (MQ=255)
                                                                                                                          ttATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGAt                      <  2:1728037/148‑1 (MQ=255)
                                                                                                                           tATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATc                     >  1:1378004/1‑148 (MQ=255)
                                                                                                                           tATTTACTACCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATc                     >  2:3212874/1‑148 (MQ=255)
                                                                                                                              ttACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                  >  1:3146796/1‑148 (MQ=255)
                                                                                                                              ttACTACCGACTTGTTCAGAGCCATAGACAGACCTGCGTCATCCAACAGACGCACAAAACCAGACTCGCTATCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGt                  <  1:1327502/148‑1 (MQ=255)
                                                                                                                               tactacCGACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTc                 <  1:1930534/148‑1 (MQ=255)
                                                                                                                                actacCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTca                <  2:1559640/148‑1 (MQ=255)
                                                                                                                                      gACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCct          <  1:1530870/148‑1 (MQ=255)
                                                                                                                                      gACTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCct          <  1:1530724/148‑1 (MQ=255)
                                                                                                                                       aCTTGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctg         <  2:2306065/148‑1 (MQ=255)
                                                                                                                                         ttGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgct       >  1:1592014/1‑148 (MQ=255)
                                                                                                                                         ttGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgct       >  1:1591500/1‑148 (MQ=255)
                                                                                                                                          tGTTCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCctgctg      <  2:1932887/148‑1 (MQ=255)
                                                                                                                                            ttCAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAg    <  1:2419952/148‑1 (MQ=255)
                                                                                                                                              cAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTc  <  1:2145915/148‑1 (MQ=255)
                                                                                                                                              cAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTc  >  2:1851678/1‑148 (MQ=255)
                                                                                                                                              cAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTc  >  2:1851234/1‑148 (MQ=255)
                                                                                                                                              cAGAGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTc  <  1:1035520/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTTCCCCTGTCTTACTATCGATGGTGTACGGCAGCGCACCATCTACGGCAGGATAACGTTCATAGACCCAAATGCCCGTTGCGCGCTGCTGACGAACGCGGTTCGGATACCCTTGCGGATCGTTATTTACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTC  >  CP009273/3110068‑3110357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑