Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,132,601 T→G 11.7% I172M (ATT→ATG)  yghU → putative S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,132,6010TG11.7% 419.6 / 55.3 171I172M (ATT→ATG) yghUputative S‑transferase
Reads supporting (aligned to +/- strand):  ref base T (70/81);  new base G (11/9);  total (81/90)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 179 total aligned reads displayed.

TTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTTA  >  CP009273/3132454‑3132748
                                                                                                                                                   |                                                                                                                                                   
ttATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGAtt                                                                                                                                                     >  1:2187487/1‑148 (MQ=255)
  aTCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGa                                                                                                                                                   <  2:830078/148‑1 (MQ=255)
   tCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAg                                                                                                                                                  >  1:553622/1‑148 (MQ=255)
   tCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAg                                                                                                                                                  <  2:1887681/148‑1 (MQ=255)
   tCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAg                                                                                                                                                  >  1:934586/1‑148 (MQ=255)
             aaaTTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCa                                                                                                                                        >  2:3277465/1‑148 (MQ=255)
                 ttGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCg                                                                                                                                    >  1:3222921/1‑148 (MQ=255)
                 ttGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCg                                                                                                                                    >  2:2949045/1‑148 (MQ=255)
                   ggCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCt                                                                                                                                  <  1:1939795/148‑1 (MQ=255)
                    gCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCtt                                                                                                                                 >  1:495143/1‑148 (MQ=255)
                    gCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCtt                                                                                                                                 <  1:2547157/148‑1 (MQ=255)
                     cTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCttt                                                                                                                                <  2:107381/148‑1 (MQ=255)
                     cTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCttt                                                                                                                                >  2:2153578/1‑148 (MQ=255)
                      tACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTa                                                                                                                               <  2:1800249/148‑1 (MQ=255)
                        cTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTAcc                                                                                                                             >  1:646575/1‑148 (MQ=255)
                        cTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTAcc                                                                                                                             >  1:1118319/1‑148 (MQ=255)
                        cTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTAcc                                                                                                                             <  1:394720/148‑1 (MQ=255)
                        cTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTAcc                                                                                                                             <  1:3173336/148‑1 (MQ=255)
                        cTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTAcc                                                                                                                             <  2:3222921/148‑1 (MQ=255)
                         ttCCTGCCGCAGGATTTGGCAAATCGTACTGAAACGATGAACTGGCTGTTATGGTTACATGGCGCGGCACCGTTCCTCGGAGGTGGTTTTTGTCAAATTAACCATTAATAACCGGTAAAGATTGAGTACGCCAACAACCCCTTTACCa                                                                                                                            >  1:2789033/1‑148 (MQ=255)
                           ccTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATg                                                                                                                          <  2:26390/148‑1 (MQ=255)
                           ccTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATg                                                                                                                          >  2:1370104/1‑148 (MQ=255)
                               ccGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAg                                                                                                                      <  2:2873547/148‑1 (MQ=255)
                                  cAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCTCCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCa                                                                                                                   <  1:2380185/148‑1 (MQ=255)
                                  cAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCa                                                                                                                   <  1:453432/148‑1 (MQ=255)
                                  cAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCa                                                                                                                   >  2:287701/1‑148 (MQ=255)
                                   aGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCaa                                                                                                                  <  1:680948/148‑1 (MQ=255)
                                   aGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCaa                                                                                                                  <  2:309957/148‑1 (MQ=255)
                                   aGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCaa                                                                                                                  <  2:1101257/148‑1 (MQ=255)
                                    ggATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACTCCATAAACCGCTTTACCATGGAAGCCaaa                                                                                                                 >  1:3056234/1‑148 (MQ=255)
                                    ggATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCaaa                                                                                                                 <  2:2019527/148‑1 (MQ=255)
                                       tttGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGt                                                                                                              <  1:1795107/148‑1 (MQ=255)
                                        ttGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTc                                                                                                             >  1:1694077/1‑148 (MQ=255)
                                        ttGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTc                                                                                                             >  2:1534398/1‑148 (MQ=255)
                                          ggCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTg                                                                                                           <  2:1400296/148‑1 (MQ=255)
                                          ggCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTg                                                                                                           <  2:1473812/148‑1 (MQ=255)
                                           gCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGc                                                                                                          <  1:1320128/148‑1 (MQ=255)
                                           gCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGc                                                                                                          <  2:25367/148‑1 (MQ=255)
                                                    aCTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGc                                                                                                 <  1:2089241/148‑1 (MQ=255)
                                                            gATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAg                                                                                         >  2:3228608/1‑148 (MQ=255)
                                                              tGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCa                                                                                       <  2:553622/148‑1 (MQ=255)
                                                              tGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCa                                                                                       <  2:2499947/148‑1 (MQ=255)
                                                               gAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCaa                                                                                      <  1:368282/148‑1 (MQ=255)
                                                               gAACTGGCTGGTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCaa                                                                                      <  2:1706268/148‑1 (MQ=255)
                                                                 aCTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACt                                                                                    <  1:777619/148‑1 (MQ=255)
                                                                 aCTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACt                                                                                    <  2:934586/148‑1 (MQ=255)
                                                                   tGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTgg                                                                                  >  2:881898/1‑148 (MQ=255)
                                                                        gTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgcag                                                                             <  1:2601583/148‑1 (MQ=255)
                                                                        gTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgcag                                                                             <  2:2849010/148‑1 (MQ=255)
                                                                        gTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgcag                                                                             <  1:1574357/148‑1 (MQ=255)
                                                                          tCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGTTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCgcagca                                                                           <  2:2221168/148‑1 (MQ=255)
                                                                              gTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAg                                                                       >  1:3022972/1‑148 (MQ=255)
                                                                              gTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAg                                                                       >  1:2293004/1‑148 (MQ=255)
                                                                              gTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAg                                                                       <  1:19536/148‑1 (MQ=255)
                                                                               ttACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGt                                                                      <  1:3112889/148‑1 (MQ=255)
                                                                               ttACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGt                                                                      <  2:1880928/148‑1 (MQ=255)
                                                                                tACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGtt                                                                     <  2:664606/148‑1 (MQ=255)
                                                                                tACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGtt                                                                     <  2:1610469/148‑1 (MQ=255)
                                                                                tACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGtt                                                                     <  1:1872095/148‑1 (MQ=255)
                                                                                  cAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTttg                                                                   >  1:817835/1‑148 (MQ=255)
                                                                                    gggCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTttgtt                                                                 >  2:243483/1‑148 (MQ=255)
                                                                                    gggCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTttgtt                                                                 <  2:1762915/148‑1 (MQ=255)
                                                                                    gggCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTttgtt                                                                 >  1:2497749/1‑148 (MQ=255)
                                                                                     ggCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTttgttg                                                                <  1:2608478/148‑1 (MQ=255)
                                                                                            cACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGa                                                         <  2:583807/148‑1 (MQ=255)
                                                                                              ccGGTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATg                                                       <  2:2891540/148‑1 (MQ=255)
                                                                                                  tCCTCGGCGGTGGTTTTGGTCACTCTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTa                                                   >  1:30634/1‑148 (MQ=255)
                                                                                                   ccTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCTTGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTac                                                  <  1:62362/148‑1 (MQ=255)
                                                                                                    cTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTaca                                                 <  2:1185527/148‑1 (MQ=255)
                                                                                                    cTCGGCGGTGGTTTTGGTCACGTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTaca                                                 <  2:1185498/148‑1 (MQ=255)
                                                                                                        gcggTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCAt                                             <  1:2949045/148‑1 (MQ=255)
                                                                                                        gcggTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCAt                                             >  2:2091327/1‑148 (MQ=255)
                                                                                                           gtggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGc                                          >  1:556041/1‑148 (MQ=255)
                                                                                                            tggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCg                                         >  2:2575334/1‑148 (MQ=255)
                                                                                                             ggtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCgg                                        <  1:2797055/148‑1 (MQ=255)
                                                                                                              gtTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGa                                       >  2:146202/1‑148 (MQ=255)
                                                                                                               ttttGGTCACTTTTACCGTTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGat                                      >  2:1697948/1‑148 (MQ=255)
                                                                                                                tttGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGata                                     >  2:2601281/1‑148 (MQ=255)
                                                                                                                tttGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGata                                     >  2:1658733/1‑148 (MQ=255)
                                                                                                                  tGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATg                                   <  2:3278268/148‑1 (MQ=255)
                                                                                                                     tCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCg                                >  1:1649602/1‑148 (MQ=255)
                                                                                                                          tttACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTg                           >  1:2743916/1‑148 (MQ=255)
                                                                                                                              ccATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCg                       >  2:220309/1‑148 (MQ=255)
                                                                                                                              ccATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCg                       <  1:1366904/148‑1 (MQ=255)
                                                                                                                              ccATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCg                       >  2:3120281/1‑148 (MQ=255)
                                                                                                                              ccATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCAGTGCGGATATGGCGATTTGGCCg                       >  2:220313/1‑148 (MQ=255)
                                                                                                                               cATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGt                      >  1:1073260/1‑148 (MQ=255)
                                                                                                                                 ttACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTgg                    >  2:1226790/1‑148 (MQ=255)
                                                                                                                                 ttACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTgg                    <  1:1041063/148‑1 (MQ=255)
                                                                                                                                    cGCACCGGTAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGttt                 <  1:250348/148‑1 (MQ=255)
                                                                                                                                           gTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACg          <  1:1264902/148‑1 (MQ=255)
                                                                                                                                           gTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACg          >  1:1198190/1‑148 (MQ=255)
                                                                                                                                            tAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgt         >  1:171771/1‑148 (MQ=255)
                                                                                                                                            tAAAGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgt         >  1:2879253/1‑148 (MQ=255)
                                                                                                                                             aaaGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtg        <  2:292219/148‑1 (MQ=255)
                                                                                                                                             aaaGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtg        <  1:1388596/148‑1 (MQ=255)
                                                                                                                                              aaGATGGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACgtgg       >  1:2569458/1‑148 (MQ=255)
                                                                                                                                                 aTTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGgtgt    <  1:2182331/148‑1 (MQ=255)
                                                                                                                                                   tGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATGAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTTa  >  2:2611508/1‑148 (MQ=255)
                                                                                                                                                   tGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTTa  >  2:3009439/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTATCTGGCGGAGAAATTTGGCTACTTCCTGCCGCAGGATTTGGCAAAGCGTACTGAAACGATGAACTGGCTGTTCTGGTTACAGGGCGCGGCACCGTTCCTCGGCGGTGGTTTTGGTCACTTTTACCATTACGCACCGGTAAAGATTGAGTACGCCATCAACCGCTTTACCATGGAAGCCAAACGTCTGCTCGACGTGCTGGATAAGCAACTGGCGCAGCATAAGTTTGTTGCGGGCGATGAGTACACCATTGCGGATATGGCGATTTGGCCGTGGTTTGGCAACGTGGTGTTA  >  CP009273/3132454‑3132748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑