Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,142,668 G→T 93.4% L55I (CTC→ATC)  yqhA ← UPF0114 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,142,6680GT93.4% 465.1 / 23.9 167L55I (CTC→ATC) yqhAUPF0114 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (6/5);  new base T (88/68);  total (94/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 172 total aligned reads displayed.

TTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCATT  >  CP009273/3142524‑3142812
                                                                                                                                                |                                                                                                                                                
ttGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACgatgat                                                                                                                                               <  1:1379465/148‑1 (MQ=255)
ttGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACgatgat                                                                                                                                               <  1:368672/148‑1 (MQ=255)
   cGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCaa                                                                                                                                            >  1:2044349/1‑148 (MQ=255)
   cGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCaa                                                                                                                                            >  1:3114310/1‑148 (MQ=255)
   cGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCaa                                                                                                                                            <  1:1203373/148‑1 (MQ=255)
   cGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCaa                                                                                                                                            <  2:2927284/148‑1 (MQ=255)
    gTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCaaa                                                                                                                                           <  2:3203281/148‑1 (MQ=255)
     tCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAAt                                                                                                                                          <  2:874557/148‑1 (MQ=255)
     tCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAAt                                                                                                                                          <  1:2586878/148‑1 (MQ=255)
     tCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAAt                                                                                                                                          <  2:3312001/148‑1 (MQ=255)
        aTTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTg                                                                                                                                       >  1:2867390/1‑148 (MQ=255)
         ttttCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGa                                                                                                                                      <  1:541590/148‑1 (MQ=255)
          tttCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGAt                                                                                                                                     >  1:400384/1‑148 (MQ=255)
          tttCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGAt                                                                                                                                     >  1:2064556/1‑148 (MQ=255)
               ccagccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGc                                                                                                                                <  2:3073048/148‑1 (MQ=255)
               ccagccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGc                                                                                                                                >  2:2176848/1‑148 (MQ=255)
                  gccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCAt                                                                                                                             >  2:2739205/1‑148 (MQ=255)
                   ccagTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATc                                                                                                                            >  2:724379/1‑148 (MQ=255)
                      gTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCCGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                         <  2:2498266/148‑1 (MQ=255)
                      gTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                         >  1:1361843/1‑148 (MQ=255)
                      gTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                         >  1:1361818/1‑148 (MQ=255)
                      gTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgag                                                                                                                         <  1:862657/148‑1 (MQ=255)
                       ttCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCgaga                                                                                                                        <  2:2019922/148‑1 (MQ=255)
                         catcTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGagaag                                                                                                                      <  1:921602/145‑1 (MQ=255)
                         cAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGagaag                                                                                                                      >  2:2433573/1‑148 (MQ=255)
                         cAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGagaag                                                                                                                      <  1:2279751/148‑1 (MQ=255)
                         cAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGagaag                                                                                                                      <  2:138484/148‑1 (MQ=255)
                           gCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGat                                                                                                                    <  2:311652/148‑1 (MQ=255)
                            cTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGata                                                                                                                   >  1:279890/1‑148 (MQ=255)
                               ctctTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATCTTc                                                                                                                >  2:2409587/1‑148 (MQ=255)
                                 ctTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCgg                                                                                                              >  1:2222364/1‑148 (MQ=255)
                                        ctcGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCACCCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACg                                                                                                       <  1:1709450/148‑1 (MQ=255)
                                           ggAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGa                                                                                                    <  1:1759198/148‑1 (MQ=255)
                                           ggAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGa                                                                                                    <  1:344863/148‑1 (MQ=255)
                                              gaTATCCAGCTGTGAGACGAAATTCTAATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaata                                                                                                 <  1:2820369/148‑1 (MQ=255)
                                               atatCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaataa                                                                                                >  2:2845024/1‑148 (MQ=255)
                                                tatCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGaataat                                                                                               <  1:612449/148‑1 (MQ=255)
                                                   ccAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAAtctc                                                                                            >  1:231764/1‑148 (MQ=255)
                                                    cAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTcc                                                                                           <  2:2643063/148‑1 (MQ=255)
                                                     aGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCt                                                                                          >  1:71565/1‑148 (MQ=255)
                                                         gtgAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaa                                                                                      >  1:2953873/1‑148 (MQ=255)
                                                           gattctaaaTTCTCATAACCGTAAAACATCACCTTCACCAGCAAACCGCCAACCAGCGTCATATGCTCCTGCGACAGAATCACGATGATCTAATCTGATTCCTCCATCGAGAAGATATTCGGCAGTAAGTGAATTATCTCCTGgaaga                                                                                    <  2:1541175/142‑1 (MQ=255)
                                                           gagaCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaaga                                                                                    <  2:1429620/148‑1 (MQ=255)
                                                            agaCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaagaa                                                                                   <  2:530934/148‑1 (MQ=255)
                                                            agaCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGgaagaa                                                                                   >  2:2540982/1‑148 (MQ=255)
                                                              aCGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACt                                                                                 >  2:2450656/1‑148 (MQ=255)
                                                               cGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCACCCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACtt                                                                                <  1:304126/148‑1 (MQ=255)
                                                                gAAATTCTCATAACCGGAAAACATCACCATCACCAGCAACCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTc                                                                               <  1:9300/148‑1 (MQ=255)
                                                                 aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCa                                                                              >  2:2809193/1‑148 (MQ=255)
                                                                 aaaTTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCa                                                                              >  2:1157455/1‑148 (MQ=255)
                                                                        cATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAg                                                                       >  1:2456133/1‑148 (MQ=255)
                                                                            aCCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCa                                                                   >  1:2118331/1‑148 (MQ=255)
                                                                                gAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTa                                                               >  2:2146411/1‑148 (MQ=255)
                                                                                   aaCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACg                                                            >  1:2222785/1‑148 (MQ=255)
                                                                                   aaCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACg                                                            >  1:347992/1‑148 (MQ=255)
                                                                                   aaCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACg                                                            >  1:312723/1‑148 (MQ=255)
                                                                                   aaCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCATGGAAGAACTTCAGCGCCAGGGCAACTAACg                                                            >  1:2222807/1‑148 (MQ=255)
                                                                                    aCATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGc                                                           <  1:1818565/148‑1 (MQ=255)
                                                                                     catcaccatcacCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGcc                                                          >  1:2848673/1‑148 (MQ=255)
                                                                                        caccatcaccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGc                                                       >  1:1240809/1‑148 (MQ=255)
                                                                                              caccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAgg                                                 >  2:1819554/1‑148 (MQ=255)
                                                                                              caccaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAgg                                                 >  2:1820511/1‑148 (MQ=255)
                                                                                               accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGc                                                >  1:2627987/1‑148 (MQ=255)
                                                                                               accaGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGc                                                >  1:2628682/1‑148 (MQ=255)
                                                                                                   gCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCaaa                                            >  1:1157276/1‑148 (MQ=255)
                                                                                                    cAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAg                                                        >  2:1523818/1‑135 (MQ=255)
                                                                                                    cAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAg                                                        <  1:1523818/135‑1 (MQ=255)
                                                                                                    cAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                           >  1:2361070/1‑148 (MQ=255)
                                                                                                    cAAACCCCCAACCAGCGTCATAACCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAg                                           <  1:877549/148‑1 (MQ=255)
                                                                                                     aaaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGt                                          <  1:1303234/148‑1 (MQ=255)
                                                                                                      aaCCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTa                                         <  2:2405381/148‑1 (MQ=255)
                                                                                                      aaCCACCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTa                                         >  1:2883976/1‑148 (MQ=255)
                                                                                                        ccGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGATAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTaca                                       >  2:1873102/1‑148 (MQ=255)
                                                                                                         cGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTacac                                      >  2:642858/1‑148 (MQ=255)
                                                                                                         cGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTacac                                      >  1:2503010/1‑148 (MQ=255)
                                                                                                          gCCAACCAGCGTCATATCCACCCGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACg                                     <  1:791247/148‑1 (MQ=255)
                                                                                                                cAGCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCa                               <  2:175300/148‑1 (MQ=255)
                                                                                                                  gCGTCATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAag                             >  1:116586/1‑148 (MQ=255)
                                                                                                                      cATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcagc                         <  2:1398650/148‑1 (MQ=255)
                                                                                                                      cATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcagc                         <  2:3190234/148‑1 (MQ=255)
                                                                                                                      cATATCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcagc                         <  1:3198937/148‑1 (MQ=255)
                                                                                                                      cATAACCACCAGCGACAGCAACACGATGATCAAATCTTATTCCGCCATCGAGAAGATATTCGGCAGTACGTTAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAagcagc                         <  1:104315/148‑1 (MQ=255)
                                                                                                                         atCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCTCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagccag                      >  1:1113627/1‑148 (MQ=255)
                                                                                                                         atCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagccag                      >  2:3303387/1‑148 (MQ=255)
                                                                                                                         atCCACCAGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGcagccag                      >  2:968204/1‑148 (MQ=255)
                                                                                                                            caccaGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCga                   <  1:750482/148‑1 (MQ=255)
                                                                                                                             accaGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCgag                  <  2:2044349/148‑1 (MQ=255)
                                                                                                                              ccaGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCgaga                 <  2:1327975/148‑1 (MQ=255)
                                                                                                                                aGCGACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAg               >  2:2571156/1‑148 (MQ=255)
                                                                                                                                   gACAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCAt            <  2:2461795/148‑1 (MQ=255)
                                                                                                                                    aCAGCAACCCGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATa           <  1:2700901/148‑1 (MQ=255)
                                                                                                                                    aCAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATa           >  1:1772434/1‑148 (MQ=255)
                                                                                                                                    aCAGCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATa           >  1:1465503/1‑148 (MQ=255)
                                                                                                                                       gCAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACAt        <  1:57391/148‑1 (MQ=255)
                                                                                                                                        cAACACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACAtt       >  2:2336712/1‑148 (MQ=255)
                                                                                                                                         aaCACGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTg      <  1:2409587/148‑1 (MQ=255)
                                                                                                                                          acacGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGc     <  1:2433737/148‑1 (MQ=255)
                                                                                                                                          acacGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGc     <  1:2433545/148‑1 (MQ=255)
                                                                                                                                             cGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAtt  >  2:42172/1‑148 (MQ=255)
                                                                                                                                             cGATGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCAtt  >  1:1642914/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTGCGTCCATTTTCCCCAGCCAGTTCAGCTTCTCTTTGTTCTCGGAGATATCCAGCTGTGAGACGAAATTCTCATAACCGGAAAACATCACCATCACCAGCAAACCGCCAACCAGCGTCATATCCACCAGCGACAGCAACACGAGGATCAAATCTGATTCCGCCATCGAGAAGATATTCGGCAGTACGTGAATAATCTCCTGGAAGAACTTCAGCGCCAGGGCAACTAACGCCAGCGAAAGGCCAAAGTACACGGGGGCAAGCAGCCAGCGAGAAGCATACATTGCATT  >  CP009273/3142524‑3142812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑