Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,184,211 C→A 92.5% S74Y (TCT→TAT)  yqiI → fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,184,2110CA92.5% 636.5 / 41.7 235S74Y (TCT→TAT) yqiIfimbrial protein
Reads supporting (aligned to +/- strand):  ref base C (9/9);  new base A (108/109);  total (117/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

ATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAG  >  CP009273/3184064‑3184357
                                                                                                                                                   |                                                                                                                                                  
aTGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCAtc                                                                                                                                                    >  1:3170245/1‑148 (MQ=255)
  gCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCAtatc                                                                                                                                                  <  2:1923926/148‑1 (MQ=255)
  gCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCAtatc                                                                                                                                                  <  2:3013698/148‑1 (MQ=255)
  gCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCAtatc                                                                                                                                                  >  2:3271179/1‑148 (MQ=255)
        cATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGgag                                                                                                                                            >  2:3182960/1‑148 (MQ=255)
            aaGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGagaaga                                                                                                                                        <  2:611280/148‑1 (MQ=255)
              ggTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAAt                                                                                                                                      <  1:2418042/148‑1 (MQ=255)
              ggTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAAt                                                                                                                                      >  1:2221885/1‑148 (MQ=255)
                    aaTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCAt                                                                                                                                <  2:942773/148‑1 (MQ=255)
                    aaTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCAt                                                                                                                                >  2:243033/1‑148 (MQ=255)
                      tACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCt                                                                                                                              >  2:737426/1‑148 (MQ=255)
                      tACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCt                                                                                                                              >  2:573968/1‑148 (MQ=255)
                         cGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCa                                                                                                                           >  2:1088085/1‑148 (MQ=255)
                         cGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATACAGGAGAAGAATACCCATCTGCa                                                                                                                           >  2:2479621/1‑148 (MQ=255)
                               cATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCa                                                                                                                     >  2:143598/1‑148 (MQ=255)
                                 ttGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCaaa                                                                                                                   <  1:2075524/148‑1 (MQ=255)
                                  tGGTTACGGACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAat                                                                                                                  <  2:1867366/148‑1 (MQ=255)
                                     ttACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAAtata                                                                                                               <  1:1649174/148‑1 (MQ=255)
                                        cGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAatg                                                                                                            >  2:908328/1‑148 (MQ=255)
                                        cGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAatg                                                                                                            >  1:1463408/1‑148 (MQ=255)
                                           aCCAAGAATCTCTATCCCCAGCGATGGTAAAAAATGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAatgatg                                                                                                         <  2:2941918/148‑1 (MQ=255)
                                              aaGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGAtgttg                                                                                                      <  1:908328/148‑1 (MQ=255)
                                                         tCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCATGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAgg                                                                                           <  1:2945888/148‑1 (MQ=255)
                                                          ccccAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGt                                                                                          >  1:898032/1‑148 (MQ=255)
                                                          ccccAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGt                                                                                          >  1:1549926/1‑148 (MQ=255)
                                                             cAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCaa                                                                                       >  2:1296930/1‑148 (MQ=255)
                                                              aGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAg                                                                                      <  2:2853331/148‑1 (MQ=255)
                                                              aGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAg                                                                                      >  1:753472/1‑148 (MQ=255)
                                                               gCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGa                                                                                     <  1:1841097/148‑1 (MQ=255)
                                                                 gATTGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACt                                                                                   <  2:2526905/148‑1 (MQ=255)
                                                                 gATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACt                                                                                   <  1:2500811/148‑1 (MQ=255)
                                                                 gATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACt                                                                                   <  1:3052473/148‑1 (MQ=255)
                                                                    ggTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACtttt                                                                                <  1:1828147/148‑1 (MQ=255)
                                                                      tAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTtat                                                                              >  2:327044/1‑148 (MQ=255)
                                                                       aaaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTtata                                                                             >  2:698146/1‑148 (MQ=255)
                                                                       aaaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTtata                                                                             >  2:2686531/1‑148 (MQ=255)
                                                                       aaaaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTtata                                                                             <  1:2688342/148‑1 (MQ=255)
                                                                         aaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAAt                                                                           >  1:3160116/1‑148 (MQ=255)
                                                                         aaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAAt                                                                           >  1:2005802/1‑148 (MQ=255)
                                                                         aaaaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAAt                                                                           >  2:3100925/1‑148 (MQ=255)
                                                                           aaGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACttt                                                                                 >  2:425982/1‑140 (MQ=255)
                                                                             ggTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCGGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGATAGCATCAGGTCAAGACTTTTATAATCaaa                                                                       >  2:1390583/1‑148 (MQ=255)
                                                                              gTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCaaaa                                                                      >  1:1798205/1‑148 (MQ=255)
                                                                               tGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTGTCTGTAATAATGCACTACCAGGCCCATATCCCGGAGAAGAATACCCATCTGCTTGGGCAAATATAATGATGTTGTGAGCATCAGGTCAAGACTTTTATAATctatac                                                                     <  2:1768864/148‑6 (MQ=255)
                                                                                gATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCa                                                                                                >  2:96369/1‑120 (MQ=255)
                                                                                gATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCa                                                                                                <  1:96369/120‑1 (MQ=255)
                                                                                 aTAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActc                                                                   <  2:104115/148‑1 (MQ=255)
                                                                                 aTAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActc                                                                   >  1:431787/1‑148 (MQ=255)
                                                                                  tAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActct                                                                  >  1:2976834/1‑148 (MQ=255)
                                                                                  tAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAActct                                                                  >  2:1262201/1‑148 (MQ=255)
                                                                                   aaaaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGAATTTTATAATCAAAACTCtt                                                                 >  2:3058156/1‑148 (MQ=255)
                                                                                    aaaTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTta                                                                <  1:1433791/148‑1 (MQ=255)
                                                                                      aTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCCGCGACAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtata                                                              >  1:3249070/1‑148 (MQ=255)
                                                                                      aTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtata                                                              >  1:1030370/1‑148 (MQ=255)
                                                                                      aTCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtata                                                              >  1:129347/1‑148 (MQ=255)
                                                                                       tCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATAc                                                             <  1:2998683/148‑1 (MQ=255)
                                                                                       tCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAGAACTCTTATAc                                                             >  2:1787417/1‑148 (MQ=255)
                                                                                       tCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATAc                                                             >  1:2074433/1‑148 (MQ=255)
                                                                                        cTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACt                                                            >  1:2758704/1‑148 (MQ=255)
                                                                                        cTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACt                                                            <  1:3303915/148‑1 (MQ=255)
                                                                                        cTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACt                                                            <  2:182757/148‑1 (MQ=255)
                                                                                              aaGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGgt                                                      <  2:1956358/148‑1 (MQ=255)
                                                                                               aGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATAATTTCGgtg                                                     >  2:873213/1‑148 (MQ=255)
                                                                                                gCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGgtgt                                                    >  2:2178338/1‑148 (MQ=255)
                                                                                                 cAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCTGTGTa                                                   >  2:1951361/1‑148 (MQ=255)
                                                                                                 cAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTa                                                   >  2:152625/1‑148 (MQ=255)
                                                                                                   gTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAAc                                                 <  1:1296930/148‑1 (MQ=255)
                                                                                                    tGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACt                                                >  2:1655361/1‑148 (MQ=255)
                                                                                                     gAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACtt                                               <  1:2236220/148‑1 (MQ=255)
                                                                                                         atTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATaa                                           <  1:143593/148‑1 (MQ=255)
                                                                                                          ttttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAAt                                          >  2:2877135/1‑148 (MQ=255)
                                                                                                           tttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATg                                         >  1:1132490/1‑148 (MQ=255)
                                                                                                            ttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATgg                                        <  2:1805523/148‑1 (MQ=255)
                                                                                                            ttATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATgg                                        <  2:2771291/148‑1 (MQ=255)
                                                                                                             tATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATggg                                       <  2:2014483/148‑1 (MQ=255)
                                                                                                              aTGAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATgggg                                      <  2:1753100/148‑1 (MQ=255)
                                                                                                               tGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGt                                     <  2:3169383/148‑1 (MQ=255)
                                                                                                                gAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGtt                                    >  1:1165839/1‑148 (MQ=255)
                                                                                                                gAATGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGtt                                    >  1:2817703/1‑148 (MQ=255)
                                                                                                                 aaTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGTCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTg                                   <  2:224866/148‑1 (MQ=255)
                                                                                                                 aaTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTg                                   >  1:2557201/1‑148 (MQ=255)
                                                                                                                  aTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGa                                  >  1:644620/1‑148 (MQ=255)
                                                                                                                  aTGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGa                                  <  2:1349105/148‑1 (MQ=255)
                                                                                                                   tGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGAt                                 <  2:1485509/148‑1 (MQ=255)
                                                                                                                   tGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGAt                                 <  2:351319/148‑1 (MQ=255)
                                                                                                                   tGTTTTCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGAt                                 <  2:1797276/148‑1 (MQ=255)
                                                                                                                     ttttCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTa                               <  1:573968/148‑1 (MQ=255)
                                                                                                                       ttCTGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATg                             <  1:2986212/148‑1 (MQ=255)
                                                                                                                          tGTAATAATGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGAtt                          <  1:298852/148‑1 (MQ=255)
                                                                                                                            taataaTGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGCTGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCt                        <  2:1253370/148‑1 (MQ=255)
                                                                                                                              ataatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTAc                      >  1:1943900/1‑148 (MQ=255)
                                                                                                                               taatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACa                     >  1:1796194/1‑148 (MQ=255)
                                                                                                                               taatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACa                     >  1:2768603/1‑148 (MQ=255)
                                                                                                                                aatGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAt                    <  1:1421475/148‑1 (MQ=255)
                                                                                                                                  tGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtct                  >  1:757648/1‑148 (MQ=255)
                                                                                                                                  tGCACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACAtct                  >  2:2256188/1‑148 (MQ=255)
                                                                                                                                    cACTACCAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTcc                >  1:3309475/1‑148 (MQ=255)
                                                                                                                                       tACCAGGCCCATATCCAGGACAAGAATACCCAACTGCATGGGCAAATATAATGATGTTGTTAGCATCTGGTCAAGACTTTTATAATCAAAACTCTTATAGTTTCGGTGTAACTTATAATCGGGTTGATTATGATTCTACATCTCCACt             >  1:491906/1‑148 (MQ=255)
                                                                                                                                         ccAGGCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTAc           >  2:1549354/1‑148 (MQ=255)
                                                                                                                                            ggCCCATATCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTa        >  1:3234636/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAG  >  CP009273/3184064‑3184357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑