Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,186,181 G→C 8.2% W205C (TGG→TGC)  yqiJ → DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,186,1810GC 8.2% 806.1 / 48.9 217W205C (TGG→TGC) yqiJDUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (98/101);  new base C (4/14);  total (102/115)
Fisher's exact test for biased strand distribution p-value = 4.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

CTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGT  >  CP009273/3186034‑3186324
                                                                                                                                                   |                                                                                                                                               
cTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTgg                                                                                                                                                 <  2:1859380/148‑1 (MQ=255)
 tGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGc                                                                                                                                                >  1:915870/1‑148 (MQ=255)
  gCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGcc                                                                                                                                               <  1:1423853/148‑1 (MQ=255)
      aGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCaa                                                                                                                                           <  2:2543607/148‑1 (MQ=255)
            aCTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGGGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTg                                                                                                                                     <  2:425510/148‑1 (MQ=255)
              tCACCTATCAATTTGGCAATATAAATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTATATGAGACAAAGTGTTAATTATTTGCCGTCTTTCTGCAACGAGATATTTGTCGGAAAATAATCCCTGGCCCCAAATATTGTa                                                                                                                                   <  2:1715063/148‑1 (MQ=255)
              tCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTa                                                                                                                                   <  2:2866740/148‑1 (MQ=255)
               cACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTaa                                                                                                                                  <  2:2740284/148‑1 (MQ=255)
                aCCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAAt                                                                                                                                 <  1:2709042/148‑1 (MQ=255)
                 ccGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAAtt                                                                                                                                >  1:1274145/1‑148 (MQ=255)
                   gATCAATTTGGCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATtta                                                                                                                              <  1:1639281/148‑1 (MQ=255)
                      cAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATttatta                                                                                                                           <  1:568169/148‑1 (MQ=255)
                        aTTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAAt                                                                                                                         <  1:1583593/148‑1 (MQ=255)
                          ttGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCa                                                                                                                       >  1:74914/1‑148 (MQ=255)
                              ccAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAg                                                                                                                   >  1:3128404/1‑148 (MQ=255)
                                aaaTACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGa                                                                                                                 >  1:687497/1‑148 (MQ=255)
                                      attattTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTta                                                                                                           >  1:1845132/1‑148 (MQ=255)
                                       ttatgTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaa                                                                                                          <  2:2160415/148‑1 (MQ=255)
                                        tattTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaat                                                                                                         >  1:1951596/1‑148 (MQ=255)
                                        tattTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaat                                                                                                         >  1:468823/1‑148 (MQ=255)
                                         attTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaata                                                                                                        <  1:2243056/148‑1 (MQ=255)
                                         attTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaata                                                                                                        <  1:1360100/148‑1 (MQ=255)
                                          tttACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaataa                                                                                                       >  1:2535484/1‑148 (MQ=255)
                                           ttACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCGTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTaataat                                                                                                      >  2:2338839/1‑148 (MQ=255)
                                             actactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATgg                                                                                                    <  1:1896912/148‑1 (MQ=255)
                                               tactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgat                                                                                                  >  1:1431099/1‑148 (MQ=255)
                                                actGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatg                                                                                                 <  1:2937560/148‑1 (MQ=255)
                                                 ctGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatga                                                                                                >  1:2667836/1‑148 (MQ=255)
                                                  tGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatgat                                                                                               <  1:1202378/148‑1 (MQ=255)
                                                   ggAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTaataat                                                                                                      <  1:1859777/140‑1 (MQ=255)
                                                    gAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGatat                                                                                             <  2:968321/148‑1 (MQ=255)
                                                       ccTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGt                                                                                          >  1:582354/1‑148 (MQ=255)
                                                         tGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTa                                                                                        >  2:1841908/1‑148 (MQ=255)
                                                            agaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAAtt                                                                                     <  1:1374470/148‑1 (MQ=255)
                                                            agaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAAtt                                                                                     <  1:2528844/148‑1 (MQ=255)
                                                            agaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGACATTGTTAAtt                                                                                     <  1:2196567/148‑1 (MQ=255)
                                                             gaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTc                                                                                    <  2:155705/148‑1 (MQ=255)
                                                              aagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCt                                                                                   >  2:803627/1‑148 (MQ=255)
                                                                ggAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCtgt                                                                                 >  2:587922/1‑148 (MQ=255)
                                                                     aaTTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcct                                                                            >  2:2082004/1‑148 (MQ=255)
                                                                      aTTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcctc                                                                           <  1:2857136/148‑1 (MQ=255)
                                                                      aTTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcctc                                                                           >  1:2440475/1‑148 (MQ=255)
                                                                      aTTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcctc                                                                           <  2:914635/148‑1 (MQ=255)
                                                                        tttCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcctcct                                                                         >  2:1783235/1‑148 (MQ=255)
                                                                        tttCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcctcct                                                                         >  1:2321842/1‑148 (MQ=255)
                                                                           cACTAAAGGAGACAAAGTGTTAATTATTGGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGa                                                                      <  2:1860535/148‑1 (MQ=255)
                                                                               aaaGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGtt                                                                  >  2:790154/1‑148 (MQ=255)
                                                                               aaaGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGtt                                                                  >  2:1597471/1‑148 (MQ=255)
                                                                               aaaGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGtt                                                                  <  2:1982200/148‑1 (MQ=255)
                                                                               aaaGGAGACAAAGGGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGtt                                                                  >  1:3045996/1‑148 (MQ=255)
                                                                                aaGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGttt                                                                 <  2:127177/148‑1 (MQ=255)
                                                                                 aGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTa                                                                >  1:2454963/1‑148 (MQ=255)
                                                                                          aGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAatta                                                       <  2:1318512/148‑1 (MQ=255)
                                                                                           gtgtTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAattat                                                      >  1:1723998/1‑148 (MQ=255)
                                                                                            tgtTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAattatt                                                     >  1:1094229/1‑148 (MQ=255)
                                                                                             gtTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTg                                                    <  1:260913/148‑1 (MQ=255)
                                                                                              ttAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGc                                                   <  1:2035175/148‑1 (MQ=255)
                                                                                               tAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGcc                                                  <  2:3218809/148‑1 (MQ=255)
                                                                                                aaTTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCg                                                 <  2:74914/148‑1 (MQ=255)
                                                                                                 attattTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGt                                                >  1:98746/1‑148 (MQ=255)
                                                                                                  ttattTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTa                                               >  1:1068873/1‑148 (MQ=255)
                                                                                                  ttattTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTa                                               <  1:2026999/148‑1 (MQ=255)
                                                                                                  ttattTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTa                                               <  1:2430476/148‑1 (MQ=255)
                                                                                                  ttattTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTa                                               >  2:1058328/1‑148 (MQ=255)
                                                                                                  ttattTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTa                                               <  1:1011150/148‑1 (MQ=255)
                                                                                                      ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCg                                                 >  2:1040351/1‑142 (MQ=255)
                                                                                                      ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCa                                           >  2:1854604/1‑148 (MQ=255)
                                                                                                      ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCa                                           >  1:2097265/1‑148 (MQ=255)
                                                                                                      ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCa                                           <  1:2357666/148‑1 (MQ=255)
                                                                                                         ccGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTc                                        >  1:333621/1‑148 (MQ=255)
                                                                                                         ccGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTc                                        <  1:1198412/148‑1 (MQ=255)
                                                                                                            aCTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGt                                     <  1:2873113/148‑1 (MQ=255)
                                                                                                            aCTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGt                                     >  2:2356167/1‑148 (MQ=255)
                                                                                                             cTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGtt                                    <  2:3176840/148‑1 (MQ=255)
                                                                                                             cTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGtt                                    <  2:3029000/148‑1 (MQ=255)
                                                                                                              tttCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGttt                                   >  2:548888/1‑148 (MQ=255)
                                                                                                              tttCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGttt                                   >  2:3252644/1‑148 (MQ=255)
                                                                                                                tCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTttat                                 >  1:324644/1‑148 (MQ=255)
                                                                                                                 cTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTttatt                                <  1:506022/148‑1 (MQ=255)
                                                                                                                 cTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTttatt                                <  2:241178/148‑1 (MQ=255)
                                                                                                                 cTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTttatt                                <  1:2440595/148‑1 (MQ=255)
                                                                                                                       cgcgATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGa                          <  2:680504/148‑1 (MQ=255)
                                                                                                                       cgcgATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGa                          >  2:1909399/1‑148 (MQ=255)
                                                                                                                        gcgATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGaa                         <  2:458160/148‑1 (MQ=255)
                                                                                                                          gATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAatt                       <  1:2021861/148‑1 (MQ=255)
                                                                                                                            tatTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAattat                     <  1:443737/148‑1 (MQ=255)
                                                                                                                            tatTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAattat                     <  2:1375627/148‑1 (MQ=255)
                                                                                                                             atTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAattatt                    <  1:2410945/148‑1 (MQ=255)
                                                                                                                             atTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAattatt                    <  2:2653006/148‑1 (MQ=255)
                                                                                                                             atTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAattatt                    >  2:1449977/1‑148 (MQ=255)
                                                                                                                             atTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAattatt                    >  2:163429/1‑148 (MQ=255)
                                                                                                                              tttGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTAttt                   <  2:2891441/148‑1 (MQ=255)
                                                                                                                                    ggAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCa             <  1:3059998/148‑1 (MQ=255)
                                                                                                                                      aaaaTAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAgg           >  2:1299391/1‑148 (MQ=255)
                                                                                                                                       aaaTAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGc          >  2:2619060/1‑148 (MQ=255)
                                                                                                                                           aatCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTa      <  2:324644/148‑1 (MQ=255)
                                                                                                                                            atCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTAt     <  1:1049043/148‑1 (MQ=255)
                                                                                                                                              cccTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTAtcg   >  1:57571/1‑148 (MQ=255)
                                                                                                                                              cccTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTAtcg   >  2:1186896/1‑148 (MQ=255)
                                                                                                                                               ccTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTAtcgt  <  1:168405/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CTGCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGT  >  CP009273/3186034‑3186324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑