Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,189,569 C→A 36.0% D182Y (GAT→TAT)  hldE ← fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,189,5690CA36.0% 226.2 / 127.8 152D182Y (GAT→TAT) hldEfused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base C (66/31);  new base A (29/26);  total (95/57)
Fisher's exact test for biased strand distribution p-value = 8.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

CACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAG  >  CP009273/3189423‑3189716
                                                                                                                                                  |                                                                                                                                                   
 aCTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCgg                                                                                                                                                   >  1:2079194/1‑148 (MQ=255)
 aCTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAgg                                                                                                                                                   <  1:1161683/148‑1 (MQ=255)
  cTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGt                                                                                                                                                  >  1:1779121/1‑148 (MQ=255)
   tAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGCTGCGGCGTTCTCAGCGTAGCGCCGCGGTCGCGCTCACAGTCGGtc                                                                                                                                                 >  1:1886803/1‑147 (MQ=255)
        gagCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCttt                                                                                                                                            >  1:1894449/1‑148 (MQ=255)
           ccGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTgg                                                                                                                                         <  2:2804549/148‑1 (MQ=255)
              agagTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatc                                                                                                                                      >  1:747377/1‑148 (MQ=255)
              agagTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatc                                                                                                                                      >  1:1966983/1‑148 (MQ=255)
              agagTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatc                                                                                                                                      >  1:1966965/1‑148 (MQ=255)
               gagTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatca                                                                                                                                     <  1:2441661/148‑1 (MQ=255)
                agTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatcaa                                                                                                                                    <  1:786462/148‑1 (MQ=255)
                agTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatcaa                                                                                                                                    <  1:786452/148‑1 (MQ=255)
                 gTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatcaat                                                                                                                                   <  1:1481412/148‑1 (MQ=255)
                  ttCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatcaatc                                                                                                                                  >  1:2379916/1‑148 (MQ=255)
                   tCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatcaatca                                                                                                                                 >  2:609803/1‑148 (MQ=255)
                   tCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatcaatca                                                                                                                                 >  2:610061/1‑148 (MQ=255)
                    cGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAg                                                                                                                                >  1:1802569/1‑148 (MQ=255)
                      tAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCa                                                                                                                              <  2:2418727/148‑1 (MQ=255)
                      tAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCa                                                                                                                              <  1:1345913/148‑1 (MQ=255)
                       aaTCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCAc                                                                                                                             >  1:1190717/1‑148 (MQ=255)
                                tCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACAcc                                                                                                                    <  2:2840032/148‑1 (MQ=255)
                                tCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGGGGGTCATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTCCCTTTTGGATCAATCAGCACCGGAACAcc                                                                                                                    >  1:1515576/1‑148 (MQ=255)
                                 cAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACAccc                                                                                                                   >  1:1977392/1‑148 (MQ=255)
                                   gTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGc                                                                                                                 <  2:305998/148‑1 (MQ=255)
                                    tttCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCt                                                                                                                >  1:1761494/1‑148 (MQ=255)
                                    tttCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCt                                                                                                                <  1:1417035/148‑1 (MQ=255)
                                     ttCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCtt                                                                                                               <  1:1576345/148‑1 (MQ=255)
                                      tCATGTCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCttt                                                                                                              <  1:2878574/148‑1 (MQ=255)
                                      tCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCttt                                                                                                              <  1:1541783/148‑1 (MQ=255)
                                         tGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTAcg                                                                                                           <  2:2492831/148‑1 (MQ=255)
                                         tGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTAcg                                                                                                           >  2:3079243/1‑148 (MQ=255)
                                         tGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTAcg                                                                                                           >  2:69436/1‑148 (MQ=255)
                                             gcgcTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGcc                                                                                                       <  1:3280898/148‑1 (MQ=255)
                                                cTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGt                                                                                                    >  2:74856/1‑148 (MQ=255)
                                                    acaaTCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGa                                                                                                >  2:1553040/1‑148 (MQ=255)
                                                     caaTCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGat                                                                                               >  1:1860137/1‑148 (MQ=255)
                                                        tctcTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcat                                                                                            <  1:3288948/148‑1 (MQ=255)
                                                        tctcTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcat                                                                                            >  1:2711398/1‑148 (MQ=255)
                                                         ctctTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcatc                                                                                           <  1:489126/148‑1 (MQ=255)
                                                                tcGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTa                                                                                    >  2:166581/1‑148 (MQ=255)
                                                                  ggTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACg                                                                                  >  1:899134/1‑148 (MQ=255)
                                                                   gTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGc                                                                                 <  2:1033529/148‑1 (MQ=255)
                                                                    tCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCt                                                                                <  1:2807792/148‑1 (MQ=255)
                                                                    tCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCt                                                                                >  1:1499745/1‑148 (MQ=255)
                                                                     cTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCtt                                                                               >  2:1453848/1‑148 (MQ=255)
                                                                      ttACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTg                                                                              >  1:478628/1‑148 (MQ=255)
                                                                       tACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGc                                                                             >  2:2930559/1‑148 (MQ=255)
                                                                       tACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTAAACAGCTTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGATGTACGCTTga                                                                             >  2:2927996/1‑147 (MQ=255)
                                                                        aCATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGcc                                                                            <  2:1802569/148‑1 (MQ=255)
                                                                          aTTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAg                                                                          >  2:3148142/1‑148 (MQ=255)
                                                                          aTTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAg                                                                          >  2:3149071/1‑148 (MQ=255)
                                                                          aTTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAg                                                                          >  1:1440799/1‑148 (MQ=255)
                                                                          aTTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTAGGATCATCTGCTGTACGCTTGCCAg                                                                          >  2:1046982/1‑148 (MQ=255)
                                                                           tttACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAgc                                                                         >  2:2870496/1‑148 (MQ=255)
                                                                                 gACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCt                                                                   <  1:3173025/148‑1 (MQ=255)
                                                                                      caGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGc                                                              >  1:2845004/1‑148 (MQ=255)
                                                                                      caGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGc                                                              >  1:2659324/1‑148 (MQ=255)
                                                                                       aGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCg                                                             <  2:3309402/148‑1 (MQ=255)
                                                                                        gCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGt                                                            <  1:1361271/148‑1 (MQ=255)
                                                                                           tCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGt                                                         >  2:2101803/1‑148 (MQ=255)
                                                                                           tCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGt                                                         >  1:1401593/1‑148 (MQ=255)
                                                                                            cAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTc                                                        <  1:3079243/148‑1 (MQ=255)
                                                                                                ttCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGaa                                                    <  2:2706205/148‑1 (MQ=255)
                                                                                                ttCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGaa                                                    >  2:1185254/1‑148 (MQ=255)
                                                                                                  ccGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAg                                                  <  2:2773676/148‑1 (MQ=255)
                                                                                                      gagaTTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTCGTCAGAAAGCAcc                                              >  2:1096041/1‑148 (MQ=255)
                                                                                                      gagaTTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCAcc                                              >  2:1096025/1‑148 (MQ=255)
                                                                                                       agaTTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCa                                             >  2:495951/1‑148 (MQ=255)
                                                                                                       agaTTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCa                                             <  2:2021165/148‑1 (MQ=255)
                                                                                                       agaTTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCa                                             <  2:2021565/148‑1 (MQ=255)
                                                                                                        gaTTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAg                                            >  2:1706254/1‑148 (MQ=255)
                                                                                                         aTTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAACACCAgc                                           >  1:2493024/1‑148 (MQ=255)
                                                                                                           tCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAgcgc                                         >  2:2514647/1‑148 (MQ=255)
                                                                                                              gCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGcc                                      >  2:1788957/1‑148 (MQ=255)
                                                                                                               cGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCa                                     >  2:2029638/1‑148 (MQ=255)
                                                                                                               cGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCa                                     >  2:362912/1‑148 (MQ=255)
                                                                                                                  tAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATc                                  >  2:2535232/1‑148 (MQ=255)
                                                                                                                    aCAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGa                                >  2:1421052/1‑148 (MQ=255)
                                                                                                                    aCAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGa                                >  1:1473898/1‑148 (MQ=255)
                                                                                                                    aCAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGa                                >  1:1413419/1‑148 (MQ=255)
                                                                                                                    aCAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGa                                <  2:443530/148‑1 (MQ=255)
                                                                                                                    aCAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGa                                <  2:1249328/148‑1 (MQ=255)
                                                                                                                        cGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTc                            >  1:212075/1‑148 (MQ=255)
                                                                                                                        cGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTc                            >  1:2810171/1‑148 (MQ=255)
                                                                                                                        cGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTc                            >  1:3269297/1‑148 (MQ=255)
                                                                                                                          tAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCAAGCGCACATTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAg                          >  1:455057/1‑148 (MQ=255)
                                                                                                                          tAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAg                          <  1:979318/148‑1 (MQ=255)
                                                                                                                           aGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAgc                         >  2:716109/1‑148 (MQ=255)
                                                                                                                             cgccgcGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAgcgc                       <  2:1385283/148‑1 (MQ=255)
                                                                                                                               ccgcGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCt                     >  1:2697427/1‑148 (MQ=255)
                                                                                                                               ccgcGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCt                     >  1:2697237/1‑148 (MQ=255)
                                                                                                                                  cgGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGAt                  <  1:2514647/148‑1 (MQ=255)
                                                                                                                                     tAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGGAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTaa               <  2:2159581/148‑1 (MQ=255)
                                                                                                                                      aGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAAt              <  1:2747315/148‑1 (MQ=255)
                                                                                                                                      aGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAAt              >  2:146637/1‑148 (MQ=255)
                                                                                                                                         gcTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCg           <  1:2398148/148‑1 (MQ=255)
                                                                                                                                         gcTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCg           <  1:2398088/148‑1 (MQ=255)
                                                                                                                                         gcTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACTCGCCAGTTGGATCATCTGCTGTTCGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCg           <  1:2400240/148‑1 (MQ=255)
                                                                                                                                            cAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTc        >  2:1865221/1‑148 (MQ=255)
                                                                                                                                               aaTAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTg     <  1:1855024/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTGCAG  >  CP009273/3189423‑3189716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑