Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,217,289 A→T 92.1% H433L (CAC→CTC)  ebgA → evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,217,2890AT92.1% 542.3 / 30.3 178H433L (CAC→CTC) ebgAevolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (10/4);  new base T (100/64);  total (110/68)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 178 total aligned reads displayed.

CTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTG  >  CP009273/3217147‑3217432
                                                                                                                                              |                                                                                                                                               
cTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACgcgc                                                                                                                                            <  2:1279702/148‑1 (MQ=255)
  gtgtgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAg                                                                                                                                          >  2:2250241/1‑148 (MQ=255)
     tgATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGaaa                                                                                                                                       >  1:3116265/1‑148 (MQ=255)
      gATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGaaaa                                                                                                                                      <  1:3313630/148‑1 (MQ=255)
         atCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAAcc                                                                                                                                   >  2:3038119/1‑148 (MQ=255)
         atCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAAcc                                                                                                                                   <  1:979307/148‑1 (MQ=255)
          tCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCa                                                                                                                                  <  2:2325724/148‑1 (MQ=255)
          tCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCa                                                                                                                                  <  2:662375/148‑1 (MQ=255)
           cTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCAt                                                                                                                                 >  2:1880171/1‑148 (MQ=255)
           cTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCAt                                                                                                                                 >  2:1879504/1‑148 (MQ=255)
           cTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCAt                                                                                                                                 <  2:2942067/148‑1 (MQ=255)
           cTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCAt                                                                                                                                 <  2:1817683/148‑1 (MQ=255)
             aCGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATcc                                                                                                                               >  1:2510781/1‑148 (MQ=255)
                 ccTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCg                                                                                                                           <  2:3191298/148‑1 (MQ=255)
                 ccTGTATGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAAACATCCGTCg                                                                                                                           >  1:1785428/1‑148 (MQ=255)
                  cTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGa                                                                                                                          >  1:2727210/1‑148 (MQ=255)
                   tGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGat                                                                                                                         <  2:2675081/148‑1 (MQ=255)
                    gTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatc                                                                                                                        >  2:907885/1‑148 (MQ=255)
                     tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatca                                                                                                                       >  2:2130557/1‑148 (MQ=255)
                     tttGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatca                                                                                                                       >  2:446/1‑148 (MQ=255)
                       tgtgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatc                                                                                                                     <  2:184860/148‑1 (MQ=255)
                         tgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcat                                                                                                                   <  1:702017/148‑1 (MQ=255)
                         tgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcat                                                                                                                   <  1:1462191/148‑1 (MQ=255)
                         tgATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcat                                                                                                                   <  1:2395112/148‑1 (MQ=255)
                          gATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGatcatcatc                                                                                                                  <  1:2511988/148‑1 (MQ=255)
                              gCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGt                                                                                                              >  1:1399762/1‑148 (MQ=255)
                                 gAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGc                                                                                                           >  2:1603632/1‑148 (MQ=255)
                                 gAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGc                                                                                                           >  2:3243620/1‑148 (MQ=255)
                                  aaaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCCTATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCt                                                                                                          >  2:2553986/1‑148 (MQ=255)
                                   aaCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTg                                                                                                         <  2:2932150/148‑1 (MQ=255)
                                    aCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTgg                                                                                                        >  2:874611/1‑148 (MQ=255)
                                    aCCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTgg                                                                                                        >  1:242349/1‑148 (MQ=255)
                                        aCGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCaa                                                                                                    <  2:1383776/148‑1 (MQ=255)
                                         cGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAAt                                                                                                   >  2:608816/1‑148 (MQ=255)
                                             gAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAAt                                                                                               <  1:1175275/148‑1 (MQ=255)
                                              aaTCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATc                                                                                              >  2:1385291/1‑148 (MQ=255)
                                                tCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCg                                                                                            >  2:2296731/1‑148 (MQ=255)
                                                  gCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGc                                                                                          <  2:2131831/148‑1 (MQ=255)
                                                  gCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGc                                                                                          <  1:767261/148‑1 (MQ=255)
                                                     cGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTAt                                                                                       <  2:3010771/148‑1 (MQ=255)
                                                            gCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTa                                                                                >  1:2872569/1‑148 (MQ=255)
                                                             cTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTaa                                                                               >  1:1711000/1‑148 (MQ=255)
                                                             cTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTaa                                                                               <  1:2016952/148‑1 (MQ=255)
                                                                aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACAt                                                                            >  1:3141662/1‑148 (MQ=255)
                                                                aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACAt                                                                            >  1:1328428/1‑148 (MQ=255)
                                                                aTGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACAt                                                                            >  2:1787979/1‑148 (MQ=255)
                                                                 tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATc                                                                           >  1:2202595/1‑148 (MQ=255)
                                                                 tGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATc                                                                           >  2:2112299/1‑148 (MQ=255)
                                                                  gTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATcc                                                                          >  1:177856/1‑148 (MQ=255)
                                                                       cGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcgcgcg                                                                     <  2:2283963/148‑1 (MQ=255)
                                                                          tatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATg                                                                  <  1:1373642/148‑1 (MQ=255)
                                                                          tatTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATg                                                                  <  1:3020770/148‑1 (MQ=255)
                                                                           atTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGt                                                                 >  1:1338574/1‑148 (MQ=255)
                                                                               gCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCa                                                             >  1:570621/1‑148 (MQ=255)
                                                                                ccGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCAt                                                            >  2:544639/1‑148 (MQ=255)
                                                                                   tATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATgcg                                                         >  2:3213280/1‑148 (MQ=255)
                                                                                        ccGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGaa                                                    <  2:3311119/148‑1 (MQ=255)
                                                                                        ccGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCAGTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGaa                                                    <  2:3310570/148‑1 (MQ=255)
                                                                                         cgacgaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGaaa                                                   >  1:1384848/1‑148 (MQ=255)
                                                                                           acgaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAgc                                                 >  1:2285950/1‑148 (MQ=255)
                                                                                            cgaTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAgcg                                                <  1:2457069/148‑1 (MQ=255)
                                                                                              aTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCt                                              <  1:1670314/148‑1 (MQ=255)
                                                                                              aTCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCt                                              >  1:572929/1‑148 (MQ=255)
                                                                                               tCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTg                                             >  2:1427769/1‑148 (MQ=255)
                                                                                               tCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTg                                             >  1:119269/1‑148 (MQ=255)
                                                                                                ccGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTgg                                            >  2:1414390/1‑148 (MQ=255)
                                                                                                ccGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTgg                                            >  2:2499394/1‑148 (MQ=255)
                                                                                                   cAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATg                                         >  1:1199485/1‑148 (MQ=255)
                                                                                                     gTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGac                                       <  2:695992/148‑1 (MQ=255)
                                                                                                     gTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGac                                       <  2:2503046/148‑1 (MQ=255)
                                                                                                      tGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGaca                                      <  1:1064980/148‑1 (MQ=255)
                                                                                                          aaaaaGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACAcgcg                                  >  1:37938/1‑148 (MQ=255)
                                                                                                           aaaaGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAACGCGCTGGATGACACGCGa                                 >  2:1253200/1‑148 (MQ=255)
                                                                                                             aaGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACt                               >  1:1780986/1‑148 (MQ=255)
                                                                                                             aaGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACt                               >  1:1586784/1‑148 (MQ=255)
                                                                                                             aaGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACt                               >  1:1585887/1‑148 (MQ=255)
                                                                                                              aGTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAACGCGCTGGATGACACGCGACTg                              >  1:820252/1‑148 (MQ=255)
                                                                                                               gTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTgg                             <  1:3014827/148‑1 (MQ=255)
                                                                                                               gTCTACGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTgg                             <  1:867529/148‑1 (MQ=255)
                                                                                                                   aCGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCa                         <  1:3271165/148‑1 (MQ=255)
                                                                                                                    cGTCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCAt                        <  1:1880171/148‑1 (MQ=255)
                                                                                                                      tCGAGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCATTGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCAGGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTa                      <  1:3213760/148‑1 (MQ=255)
                                                                                                                         aGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACga                   <  1:2460328/148‑1 (MQ=255)
                                                                                                                         aGCGCATTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATAATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACga                   >  1:479589/1‑148 (MQ=255)
                                                                                                                              aTTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATTACACGCGACTGGTGCATTACGaagaag              <  1:2250241/148‑1 (MQ=255)
                                                                                                                              aTTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGaagaag              <  1:643599/148‑1 (MQ=255)
                                                                                                                              aTTGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGaagaag              <  1:3226947/148‑1 (MQ=255)
                                                                                                                               ttGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAagaaga             <  1:2434273/148‑1 (MQ=255)
                                                                                                                                tGTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGAt            >  1:1034157/1‑148 (MQ=255)
                                                                                                                                 gTTCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATc           >  2:3077506/1‑148 (MQ=255)
                                                                                                                                  ttCGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcg          >  2:3196193/1‑148 (MQ=255)
                                                                                                                                    cGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgcg        >  1:566346/1‑148 (MQ=255)
                                                                                                                                    cGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgcg        >  1:2447250/1‑148 (MQ=255)
                                                                                                                                    cGCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATcgcg        >  2:65316/1‑148 (MQ=255)
                                                                                                                                     gCCATATCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGa       >  1:2367863/1‑148 (MQ=255)
                                                                                                                                         tatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCt   >  1:2471879/1‑148 (MQ=255)
                                                                                                                                         tatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCt   >  1:2471882/1‑148 (MQ=255)
                                                                                                                                         tatCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCt   >  2:1571011/1‑148 (MQ=255)
                                                                                                                                          atCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTg  >  1:2039685/1‑148 (MQ=255)
                                                                                                                                          atCCTCGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTCGTGCATTACGAAGAAGATCTCGATGCTg  >  1:2885506/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTG  >  CP009273/3217147‑3217432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑