Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,228,343 G→A 10.9% R298C (CGC→TGC)  rlmG ← 23S rRNA m(2)G1835 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,228,3430GA10.9% 615.7 / 47.6 192R298C (CGC→TGC) rlmG23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (81/90);  new base A (13/8);  total (94/98)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 199 total aligned reads displayed.

GTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTCGCCTGCGGGTTTTT  >  CP009273/3228197‑3228490
                                                                                                                                                  |                                                                                                                                                   
gTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCgg                                                                                                                                                    >  1:1697680/1‑148 (MQ=255)
gTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCgg                                                                                                                                                    >  2:604491/1‑148 (MQ=255)
  aaTCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGaa                                                                                                                                                  <  2:2826349/148‑1 (MQ=255)
   aTCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGaaa                                                                                                                                                 <  2:509527/148‑1 (MQ=255)
   aTCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCAGaaa                                                                                                                                                 >  1:835777/1‑148 (MQ=255)
    tCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAg                                                                                                                                                <  2:229637/148‑1 (MQ=255)
    tCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAg                                                                                                                                                <  1:725915/148‑1 (MQ=255)
    tCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAg                                                                                                                                                <  1:725784/148‑1 (MQ=255)
      cAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGc                                                                                                                                              <  2:3234202/148‑1 (MQ=255)
      cAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGc                                                                                                                                              <  2:3233527/148‑1 (MQ=255)
       aGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCt                                                                                                                                             <  1:1813632/148‑1 (MQ=255)
        ggTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTc                                                                                                                                            >  2:229566/1‑148 (MQ=255)
            aCGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACg                                                                                                                                        >  2:2766408/1‑148 (MQ=255)
                ttGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCgg                                                                                                                                    >  2:2030081/1‑148 (MQ=255)
                ttGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGGGGTGGAACATCTCCCAGGCGACGTTATCGGTCCGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCgg                                                                                                                                    >  2:364688/1‑148 (MQ=255)
                 tGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGga                                                                                                                                   >  1:1491141/1‑148 (MQ=255)
                    cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGagag                                                                                                                                >  1:1834252/1‑148 (MQ=255)
                    cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGagag                                                                                                                                >  2:1047228/1‑148 (MQ=255)
                         atatACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCgt                                                                                                                           >  1:2904217/1‑148 (MQ=255)
                          tataCAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCgtt                                                                                                                          <  2:2082540/148‑1 (MQ=255)
                           ataCAGCTCGCCGTTGTTTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCgttg                                                                                                                         <  2:2929565/148‑1 (MQ=255)
                           ataCAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCgttg                                                                                                                         <  2:1912496/148‑1 (MQ=255)
                            taCAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCgttgt                                                                                                                        <  1:1451333/148‑1 (MQ=255)
                             aCAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCgttgtt                                                                                                                       >  1:185457/1‑148 (MQ=255)
                                 cTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATc                                                                                                                   >  1:1083657/1‑148 (MQ=255)
                                    gCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATa                                                                                                                >  1:2535250/1‑148 (MQ=255)
                                    gCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATa                                                                                                                >  1:850413/1‑148 (MQ=255)
                                      cGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCAGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATaaa                                                                                                              >  2:1229447/1‑148 (MQ=255)
                                      cGTTGATTTTCAGGCAGCGGCGGGAGTGGTGTAACATCTCCCTGGCGACGTTATCGGTCAGCGCTTGTTTTTGGTTAAACGGCGGGTTGCAGAGCACATCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCTTaaa                                                                                                              <  1:110446/148‑1 (MQ=255)
                                             tttCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAg                                                                                                       >  2:2763266/1‑148 (MQ=255)
                                                 aGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGAt                                                                                                   <  2:1452187/148‑1 (MQ=255)
                                                 aGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGAt                                                                                                   <  1:2935249/148‑1 (MQ=255)
                                                    cAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCa                                                                                                <  2:1823757/148‑1 (MQ=255)
                                                     aGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCaa                                                                                               <  1:2654421/148‑1 (MQ=255)
                                                       cggcggGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCCGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACg                                                                                             >  2:1306860/1‑148 (MQ=255)
                                                        ggcgggcgTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGc                                                                                            <  2:2639885/148‑1 (MQ=255)
                                                        ggcgggcgTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGc                                                                                            <  1:2949/148‑1 (MQ=255)
                                                         gcgggcgTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGcc                                                                                           >  2:898972/1‑148 (MQ=255)
                                                            ggcgTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCctct                                                                                        <  1:534666/148‑1 (MQ=255)
                                                                  gtggAACATCTCCCAGGCGACGTTATCGGTCCGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATg                                                                                  >  2:1565193/1‑148 (MQ=255)
                                                                  gtggAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATg                                                                                  >  1:2908335/1‑148 (MQ=255)
                                                                      aaCATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTgg                                                                              >  2:115789/1‑148 (MQ=255)
                                                                      aaCATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTgg                                                                              >  2:445022/1‑148 (MQ=255)
                                                                      aaCATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCAGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTgg                                                                              <  1:2984236/148‑1 (MQ=255)
                                                                        cATCTCCCCGGCGACGTTATCGGTCAGCGATTGTTGTTGGTCAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGtt                                                                            <  1:632230/148‑1 (MQ=255)
                                                                        cATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGtt                                                                            <  1:494524/148‑1 (MQ=255)
                                                                        cATCGCCCTGGCGACGTTATCGGTCAGCGCTTGTTGTTGGTGAAACGGCGGGTTGCATAGCACAGCATTAAAGCGGACAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGtt                                                                            <  1:3184561/148‑1 (MQ=255)
                                                                         aTCTCCCACGAGACGTTATCGGTCAGCGCATGGTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATAAACGCGGAAAGGCTCCACGCCGGATAGCGCGTTGTTTATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGttt                                                                           <  1:177362/148‑1 (MQ=255)
                                                                           ctcCCAGGCGACTTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCa                                                                         <  1:2831662/148‑1 (MQ=255)
                                                                           ctcCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCa                                                                         >  1:1044774/1‑148 (MQ=255)
                                                                           ctcCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCa                                                                         >  1:713762/1‑148 (MQ=255)
                                                                            tcCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCaa                                                                        <  2:2385596/148‑1 (MQ=255)
                                                                                 ggCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTc                                                                   >  2:719310/1‑148 (MQ=255)
                                                                                   cGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCaa                                                                 <  2:536514/148‑1 (MQ=255)
                                                                                    gACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCaaa                                                                <  1:1433939/148‑1 (MQ=255)
                                                                                        ttATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGc                                                            >  2:2938462/1‑148 (MQ=255)
                                                                                        ttATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCAAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGc                                                            >  1:2224075/1‑148 (MQ=255)
                                                                                         tATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCt                                                           <  1:1100001/148‑1 (MQ=255)
                                                                                         tATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCt                                                           >  1:2495443/1‑148 (MQ=255)
                                                                                          aTCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTg                                                          >  2:2135408/1‑148 (MQ=255)
                                                                                           tCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTgg                                                         <  2:3163163/148‑1 (MQ=255)
                                                                                                cAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCAGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCa                                                    >  1:612224/1‑148 (MQ=255)
                                                                                                   cgcATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCAGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAAcc                                                 >  2:1936229/1‑148 (MQ=255)
                                                                                                     cATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGc                                               <  1:2125265/148‑1 (MQ=255)
                                                                                                      aTGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGcc                                              <  2:2970198/148‑1 (MQ=255)
                                                                                                        gttgttGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGACGCAACCGCCaa                                            >  1:2022767/1‑147 (MQ=255)
                                                                                                          tgttgGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCg                                          >  1:2281177/1‑148 (MQ=255)
                                                                                                              ggTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGa                                      <  1:229566/148‑1 (MQ=255)
                                                                                                                  aaaGGGCGGGTTGCAGAGCACAGAATTAACGCGGAAAGGCTCCACGCCGGAGCGCGGGTTGTTGATCATAAACTCGCAGCGAGCAAACGCCTGTGGCATGTTGGTTTCAACTTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCa                                  <  1:1130149/148‑1 (MQ=255)
                                                                                                                  aaaCGGCGGGTTGCATAGCACAGCATTAAAGCGGAAAGGCTCCACGCGGGAGAGCTAGTTGTTGATCATAAAATCGCATCTATCCAAGGCCTCTGTCATGTTGGTTTCATAGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCa                                  <  2:1690913/148‑1 (MQ=255)
                                                                                                                  aaaCGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCa                                  <  2:1872121/148‑1 (MQ=255)
                                                                                                                  aaaCGGCGGGTTGCAGAGCACAGCATTAAAGCAGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCa                                  <  1:1053692/148‑1 (MQ=255)
                                                                                                                  aaaCGGCGGGTTGCAGAGCACAGCATAAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATAATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCa                                  <  1:1130101/148‑1 (MQ=255)
                                                                                                                   aaCGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCAt                                 <  1:545907/148‑1 (MQ=255)
                                                                                                                   aaCGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCAt                                 >  1:3104567/1‑148 (MQ=255)
                                                                                                                   aaCGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCAt                                 >  1:1847320/1‑148 (MQ=255)
                                                                                                                    aCGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATc                                >  1:2966934/1‑148 (MQ=255)
                                                                                                                     cggcggGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCg                               <  2:680888/148‑1 (MQ=255)
                                                                                                                     cggcggGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCg                               >  2:3058715/1‑148 (MQ=255)
                                                                                                                     cggcggGTTGCAGAGCACAGCATTAAAGCAGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCg                               >  1:1854988/1‑148 (MQ=255)
                                                                                                                          ggTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACaaa                          <  1:2086989/148‑1 (MQ=255)
                                                                                                                            ttGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAaca                        >  2:1181817/1‑148 (MQ=255)
                                                                                                                            ttGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAaca                        >  2:1181809/1‑148 (MQ=255)
                                                                                                                             tGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAacac                       <  1:364688/148‑1 (MQ=255)
                                                                                                                             tGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAacac                       <  1:2030081/148‑1 (MQ=255)
                                                                                                                              gCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAAcacc                      <  1:156851/148‑1 (MQ=255)
                                                                                                                               cAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAAcacca                     <  1:1260398/148‑1 (MQ=255)
                                                                                                                                agagCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAAcaccac                    <  2:944660/148‑1 (MQ=255)
                                                                                                                                agagCACAGCATTAAAGCAGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAAcaccac                    <  2:835777/148‑1 (MQ=255)
                                                                                                                                  agCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACtt                  >  1:1988838/1‑148 (MQ=255)
                                                                                                                                    cacaGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTc                >  1:1334831/1‑148 (MQ=255)
                                                                                                                                    cacaGCATTAAAGCAGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTc                >  2:717742/1‑148 (MQ=255)
                                                                                                                                      caGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTCGc              >  1:236487/1‑148 (MQ=255)
                                                                                                                                            tAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTCGCCTGCgg        >  1:1328808/1‑148 (MQ=255)
                                                                                                                                             aaaGCGGAAAGGCTCCACGCAGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTCGCCTGCggg       <  1:2680558/148‑1 (MQ=255)
                                                                                                                                              aaGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCAGAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTCGCCTGCGGGt      <  1:1928920/148‑1 (MQ=255)
                                                                                                                                               aGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTCGCCTGCGGGtt     <  1:1464894/148‑1 (MQ=255)
                                                                                                                                                gCGGAAAGGCTCCACGCGGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTCGCCTGCGGGttt    <  1:2772145/148‑1 (MQ=255)
                                                                                                                                                gCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTCGCCTGCGGGttt    <  2:1621872/148‑1 (MQ=255)
                                                                                                                                                  ggAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTCGCCTGCGGGttttt  <  1:3271239/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAGGCGACGTTATCGGTCAGCGCATGTTGTTGGTGAAACGGCGGGTTGCAGAGCACAGCATTAAAGCGGAAAGGCTCCACGCCGGAGAGCGCGTTGTTGATCATAAACTCGCAGCGATCCAACGCCTCTGGCATGTTGGTTTCAACGTTCAAACGGCTGGAAGCAACCGCCATCGGCGATTCATCGACAAACACCACTTTCGCCTGCGGGTTTTT  >  CP009273/3228197‑3228490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑