Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,236,592 C→A 9.2% D28Y (GAT→TAT)  uxaA ← altronate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,236,5920CA 9.2% 797.4 / 34.0 231D28Y (GAT→TAT) uxaAaltronate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (97/112);  new base A (10/12);  total (107/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

GAACGTGAACACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTT  >  CP009273/3236448‑3236739
                                                                                                                                                |                                                                                                                                                   
gaacgtgaacACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGac                                                                                                                                                  >  1:250550/1‑148 (MQ=255)
gaacgtgaacACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGac                                                                                                                                                  >  1:2049990/1‑148 (MQ=255)
gaacgtgaacACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATAGac                                                                                                                                                  >  2:973923/1‑148 (MQ=255)
  acgtgaacACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGacac                                                                                                                                                <  1:1750178/148‑1 (MQ=255)
   cgtgaacACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACt                                                                                                                                               >  1:2693304/1‑148 (MQ=255)
    gtgaacACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTg                                                                                                                                              <  1:3022041/148‑1 (MQ=255)
    gtgaacACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTg                                                                                                                                              <  1:3022268/148‑1 (MQ=255)
      gaacACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGAc                                                                                                                                            <  2:224931/148‑1 (MQ=255)
        acacCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACtt                                                                                                                                          <  2:947842/148‑1 (MQ=255)
        acacCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACtt                                                                                                                                          <  2:822872/148‑1 (MQ=255)
        acacCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACtt                                                                                                                                          <  2:1804980/148‑1 (MQ=255)
           ccTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCtg                                                                                                                                       >  1:2485210/1‑148 (MQ=255)
            cTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCtgt                                                                                                                                      >  1:1910685/1‑148 (MQ=255)
                cGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCt                                                                                                                                  >  2:242023/1‑148 (MQ=255)
                 gCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCtt                                                                                                                                 <  1:1723629/148‑1 (MQ=255)
                  cAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTc                                                                                                                                <  2:2871398/148‑1 (MQ=255)
                      atCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGcc                                                                                                                            >  2:1991359/1‑148 (MQ=255)
                         cGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCaaa                                                                                                                         >  1:973257/1‑148 (MQ=255)
                          gCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAAt                                                                                                                        <  1:71886/148‑1 (MQ=255)
                           ccAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATc                                                                                                                       >  2:1744773/1‑148 (MQ=255)
                            cAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCt                                                                                                                      >  1:2507707/1‑148 (MQ=255)
                             aaTGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTg                                                                                                                     >  2:2385477/1‑148 (MQ=255)
                             aaTGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTg                                                                                                                     >  2:725885/1‑148 (MQ=255)
                             aaTGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTg                                                                                                                     >  2:1938436/1‑148 (MQ=255)
                                 cATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTaa                                                                                                                 <  1:1819942/148‑1 (MQ=255)
                                 cATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTaa                                                                                                                 <  2:2439390/148‑1 (MQ=255)
                                    aaCCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGc                                                                                                              >  2:1031693/1‑148 (MQ=255)
                                    aaCCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGc                                                                                                              >  2:895218/1‑148 (MQ=255)
                                    aaCCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGc                                                                                                              >  2:2582589/1‑148 (MQ=255)
                                    aaCCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGc                                                                                                              >  1:3000794/1‑148 (MQ=255)
                                             gCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGAc                                                                                                     >  2:1227911/1‑148 (MQ=255)
                                               aGGCCATATTTGATGACAGTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGt                                                                                                   <  2:2753210/148‑1 (MQ=255)
                                                  ccATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTAt                                                                                                >  2:2091129/1‑148 (MQ=255)
                                                           atgaCATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCAt                                                                                       >  1:2844657/1‑148 (MQ=255)
                                                             gaCATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATgg                                                                                     >  1:73778/1‑148 (MQ=255)
                                                              aCATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGa                                                                                    <  2:1824422/148‑1 (MQ=255)
                                                                 ttGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCt                                                                                 >  2:1548434/1‑148 (MQ=255)
                                                                  tGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCAACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGATAAAGCGACCGCGACTTTATACAGCGCATGGATCtt                                                                                >  1:2520406/1‑148 (MQ=255)
                                                                   ggCCCCTTTTGCTATACCCGTTACCGCAATTTTATGTCCACGACCACCATCCTGGCGCAGCGTAACTGTGTGGTTATCGACACTGACTTGTGGGCCTTCAGCCAAATCTCCAAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTg                                                                               <  1:2879440/148‑1 (MQ=255)
                                                                    gCCCCTTTTGCGATATCCGTTAACTCAAATTTATGACCACGAGAAACATCCTGGCGCAGCGAAACAGTCTGGTTATAGACACTGACTTCTGGGCCTTCCGCAAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGa                                                                              <  1:1729855/148‑1 (MQ=255)
                                                                    gCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGa                                                                              >  2:1262109/1‑148 (MQ=255)
                                                                     ccccTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGAt                                                                             >  2:696531/1‑148 (MQ=255)
                                                                     ccccTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGAt                                                                             <  2:637931/148‑1 (MQ=255)
                                                                     ccccTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGAt                                                                             >  2:3021027/1‑148 (MQ=255)
                                                                     ccccTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATAGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGAt                                                                             >  1:479360/1‑148 (MQ=255)
                                                                         ttttGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTAt                                                                         <  2:1595186/148‑1 (MQ=255)
                                                                          tttGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTAtt                                                                        >  1:1567899/1‑148 (MQ=255)
                                                                              cGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCat                                                                    >  1:501994/1‑148 (MQ=255)
                                                                                atatCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCatat                                                                  >  1:282084/1‑148 (MQ=255)
                                                                                  atCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCa                                                                >  1:2318760/1‑148 (MQ=255)
                                                                                  atCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATAGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCa                                                                <  2:2485654/148‑1 (MQ=255)
                                                                                     cGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATAGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAAcc                                                             >  1:1350755/1‑148 (MQ=255)
                                                                                        tAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCa                                                          >  2:2023411/1‑148 (MQ=255)
                                                                                         aaCGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAg                                                         <  2:1653825/148‑1 (MQ=255)
                                                                                         aaCGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAg                                                         <  2:1862227/148‑1 (MQ=255)
                                                                                           cgaaaaTTTATGTCCACGAGAAACATCCTGGCGCAGCGTAACTGTCTGTTTATCGACACTGACTTGTGTGCCTTCAGCAAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGAc                                                       <  1:2077639/145‑1 (MQ=255)
                                                                                            gCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGAcc                                                      <  2:3308987/148‑1 (MQ=255)
                                                                                             cAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCt                                                     >  1:348297/1‑148 (MQ=255)
                                                                                                 tttATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATAGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTagt                                                 >  2:1511265/1‑148 (MQ=255)
                                                                                                 tttATGTCCACGAGCAACAGCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTagt                                                 <  2:2781295/148‑1 (MQ=255)
                                                                                                   tATGTCCACGAGCAACATCCTGGCGCAGCTTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTagttc                                               <  2:3161037/148‑1 (MQ=255)
                                                                                                   tATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTagttc                                               <  2:2606153/148‑1 (MQ=255)
                                                                                                   tATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTagttc                                               <  2:1413687/148‑1 (MQ=255)
                                                                                                     tGTCCACGAGCAAAAGCCTGGCGCTGCGTAACTGTGTGTTTATCGACACTTAATTCTGTGCCTTCAGCAAAATCTGATAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTagttcag                                             <  1:613517/148‑1 (MQ=255)
                                                                                                           cGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAAt                                       >  2:254144/1‑148 (MQ=255)
                                                                                                               cAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCg                                   >  2:240689/1‑148 (MQ=255)
                                                                                                               cAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCg                                   >  2:2477939/1‑148 (MQ=255)
                                                                                                                  cATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAag                                <  2:2516763/148‑1 (MQ=255)
                                                                                                                  cATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGAGAag                                >  1:881907/1‑148 (MQ=255)
                                                                                                                   aTCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAagt                               <  2:3191938/148‑1 (MQ=255)
                                                                                                                   aTCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAagt                               <  2:3252346/148‑1 (MQ=255)
                                                                                                                     ccTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAagtag                             >  2:1107895/1‑148 (MQ=255)
                                                                                                                      cTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAagtagt                            <  2:1356968/148‑1 (MQ=255)
                                                                                                                      cTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAagtagt                            <  2:43728/148‑1 (MQ=255)
                                                                                                                       tGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGANGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTc                           >  1:1679636/1‑148 (MQ=255)
                                                                                                                       tGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTc                           <  1:2173264/148‑1 (MQ=255)
                                                                                                                       gggCGCTGCTTAACAGTGTGTTTATCGAAACTTACTTCTGTGCCTTCTCCAAAATCTGCAAACGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTc                           <  1:4777/147‑1 (MQ=255)
                                                                                                                          cgccgcGAAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCAcg                        <  1:1460479/148‑1 (MQ=255)
                                                                                                                              gCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCa                    <  2:2885173/148‑1 (MQ=255)
                                                                                                                               cGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCCGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCAt                   <  1:3156148/148‑1 (MQ=255)
                                                                                                                               cGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCAt                   <  2:87797/148‑1 (MQ=255)
                                                                                                                                 tAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTg                 >  1:1986954/1‑148 (MQ=255)
                                                                                                                                 tAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTg                 <  1:1031372/148‑1 (MQ=255)
                                                                                                                                  aaCAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGt                >  1:2323037/1‑148 (MQ=255)
                                                                                                                                  aaCAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGt                >  1:1115745/1‑148 (MQ=255)
                                                                                                                                  aaCAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGt                <  1:2091129/148‑1 (MQ=255)
                                                                                                                                     aGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCCGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTaa             <  1:1229820/148‑1 (MQ=255)
                                                                                                                                     aGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTaa             <  2:3135147/148‑1 (MQ=255)
                                                                                                                                     aGTCTGGTTATCGACACTGACTTCGGGGCCTACCCCCAAATCTGATAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTaa             <  1:9278/148‑1 (MQ=255)
                                                                                                                                       tCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAg           <  1:364469/148‑1 (MQ=255)
                                                                                                                                          ggTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCaa        >  1:1067963/1‑148 (MQ=255)
                                                                                                                                            ttATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAat      <  2:2091874/148‑1 (MQ=255)
                                                                                                                                            ttATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAat      <  2:2972805/148‑1 (MQ=255)
                                                                                                                                            ttATAGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAat      <  2:911414/148‑1 (MQ=255)
                                                                                                                                             tataGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAata     >  1:1147526/1‑148 (MQ=255)
                                                                                                                                             tATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAata     <  2:2507707/148‑1 (MQ=255)
                                                                                                                                              aTCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAatat    <  2:2651585/148‑1 (MQ=255)
                                                                                                                                              aTCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAatat    <  2:282084/148‑1 (MQ=255)
                                                                                                                                              aTCGACACTGACTTCTGTGCCTTCAGCCAAATCTGATAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAatat    <  1:2438946/148‑1 (MQ=255)
                                                                                                                                                cGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATAttt  <  2:514018/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GAACGTGAACACCTGCCGCAATATCCGCCAATGCATAACCAATCGGCAGGCCATATTTGATGACATTGGCCCCTTTTGCGATATCCGTTAACGCAAATTTATGTCCACGAGCAACATCCTGGCGCAGCGTAACAGTCTGGTTATCGACACTGACTTCTGTGCCTTCAGCCAAATCTGCTAAAGCGACCGCGACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTT  >  CP009273/3236448‑3236739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑