Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,252,258 C→A 100% D251Y (GAT→TAT)  tdcG ← L‑serine dehydratase 3, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,252,2580CA100.0% 554.9 / NA 167D251Y (GAT→TAT) tdcGL‑serine dehydratase 3, anaerobic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (98/69);  total (98/69)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 177 total aligned reads displayed.

AAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGC  >  CP009273/3252114‑3252402
                                                                                                                                                |                                                                                                                                                
aaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCt                                                                                                                                               >  2:1706772/1‑148 (MQ=255)
aaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCt                                                                                                                                               >  2:788998/1‑148 (MQ=255)
aaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGGTAGAGCTGTTATAGCt                                                                                                                                               >  2:2050458/1‑148 (MQ=255)
aaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGGTAGAGATGTTATAGCt                                                                                                                                               >  2:385962/1‑148 (MQ=255)
 aGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTg                                                                                                                                              >  1:1638954/1‑148 (MQ=255)
   ccAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGa                                                                                                                                            >  1:1601665/1‑148 (MQ=255)
     aGTACGGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGaaa                                                                                                                                          <  2:685245/148‑1 (MQ=255)
      gTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAc                                                                                                                                         <  1:82744/148‑1 (MQ=255)
       tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAcc                                                                                                                                        >  1:476463/1‑148 (MQ=255)
         cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAg                                                                                                                                      >  2:2003695/1‑148 (MQ=255)
         cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAg                                                                                                                                      >  1:1313914/1‑148 (MQ=255)
         cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAg                                                                                                                                      >  1:1217065/1‑148 (MQ=255)
         cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAg                                                                                                                                      >  1:2195294/1‑148 (MQ=255)
          tGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGc                                                                                                                                     <  2:1893760/148‑1 (MQ=255)
          tGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGc                                                                                                                                     <  1:3080341/148‑1 (MQ=255)
               gAATACTGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTgacg                                                                                                                                <  2:2821058/148‑1 (MQ=255)
                aataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTgacga                                                                                                                               <  1:501709/148‑1 (MQ=255)
                  taatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGacgacg                                                                                                                             <  1:3152462/148‑1 (MQ=255)
                    atGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCa                                                                                                                           >  2:1398705/1‑148 (MQ=255)
                     tGCCGCACGCACCGTTAGTCGGTGCCGTTACCCCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAg                                                                                                                          <  1:2045721/148‑1 (MQ=255)
                          cacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCCGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTa                                                                                                                     >  2:3153982/1‑148 (MQ=255)
                           acgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTac                                                                                                                    >  2:3202613/1‑148 (MQ=255)
                                ccGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCCGCGCGTACATGTTGATCCAGTCGATGAAAATCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGTAGCAGCGCTacggcac                                                                                                               >  1:1368709/1‑148 (MQ=255)
                                  gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTacggcacgg                                                                                                             <  2:266149/148‑1 (MQ=255)
                                         ggTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGca                                                                                                      >  2:3261444/1‑148 (MQ=255)
                                          gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGcac                                                                                                     >  1:523445/1‑148 (MQ=255)
                                             ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACAtt                                                                                                  >  2:3143952/1‑148 (MQ=255)
                                                ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTACCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAg                                                                                               <  2:2205902/148‑1 (MQ=255)
                                                ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTCGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAg                                                                                               >  1:2719431/1‑148 (MQ=255)
                                                  aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGTCGCAGCGCTACGGCACGGCGCGGCACATTGAGCg                                                                                             >  2:544050/1‑148 (MQ=255)
                                                        cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAg                                                                                       >  2:1159614/1‑148 (MQ=255)
                                                          cccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgc                                                                                     <  1:773852/148‑1 (MQ=255)
                                                            cgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgcag                                                                                   >  2:270893/1‑148 (MQ=255)
                                                              ccAGCTGCGTTTTCTTCACTAACCGCCCGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGgcagca                                                                                 >  1:382477/1‑148 (MQ=255)
                                                                aGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACg                                                                               >  2:161872/1‑148 (MQ=255)
                                                                aGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACg                                                                               <  2:2029536/148‑1 (MQ=255)
                                                                 gCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACAATGAGCGGACCAGGCAGCACGc                                                                              >  1:1994930/1‑148 (MQ=255)
                                                                  cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGcc                                                                             <  1:3056458/148‑1 (MQ=255)
                                                                  cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGcc                                                                             <  2:1973567/148‑1 (MQ=255)
                                                                    gCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCtt                                                                           >  2:324830/1‑148 (MQ=255)
                                                                      gTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCa                                                                         <  1:1587292/148‑1 (MQ=255)
                                                                       ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAg                                                                        <  1:815789/148‑1 (MQ=255)
                                                                       ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAg                                                                        <  2:789666/148‑1 (MQ=255)
                                                                        tttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAgt                                                                       >  2:426899/1‑148 (MQ=255)
                                                                          tcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAgtgt                                                                     >  2:2698266/1‑148 (MQ=255)
                                                                          tcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAgtgt                                                                     >  1:2470814/1‑148 (MQ=255)
                                                                            ttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTc                                                                   <  2:2808100/148‑1 (MQ=255)
                                                                              cACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCAt                                                                 >  1:673017/1‑148 (MQ=255)
                                                                                cTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATcc                                                               <  1:1581049/148‑1 (MQ=255)
                                                                                 tAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATccc                                                              >  2:2556310/1‑148 (MQ=255)
                                                                                  aaCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTTGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCa                                                             <  2:2386002/148‑1 (MQ=255)
                                                                                  aaCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCa                                                             >  1:2787808/1‑148 (MQ=255)
                                                                                  aaCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTAATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCa                                                             >  1:1132457/1‑148 (MQ=255)
                                                                                    ccGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACg                                                           >  1:2917997/1‑148 (MQ=255)
                                                                                    ccGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACg                                                           <  2:148596/148‑1 (MQ=255)
                                                                                      gCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGtt                                                         <  2:1276895/148‑1 (MQ=255)
                                                                                         aGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACTAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCaa                                                      <  2:3156080/148‑1 (MQ=255)
                                                                                         aGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCaa                                                      <  1:1173515/148‑1 (MQ=255)
                                                                                          gcgcGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAt                                                     >  1:2304099/1‑148 (MQ=255)
                                                                                          gcgcGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAt                                                     >  1:2303207/1‑148 (MQ=255)
                                                                                          gcgcGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAAt                                                     >  1:2211494/1‑148 (MQ=255)
                                                                                                aCATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTc                                               <  1:806534/148‑1 (MQ=255)
                                                                                                   tGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATg                                            <  2:1822746/148‑1 (MQ=255)
                                                                                                    gTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGc                                           >  1:1302927/1‑148 (MQ=255)
                                                                                                         tCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCAc                                      >  1:3240107/1‑148 (MQ=255)
                                                                                                            aGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTg                                   <  2:1161578/148‑1 (MQ=255)
                                                                                                            aGTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTg                                   <  1:821660/148‑1 (MQ=255)
                                                                                                             gTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGc                                  <  1:2603928/148‑1 (MQ=255)
                                                                                                             gTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGc                                  <  1:2698822/148‑1 (MQ=255)
                                                                                                             gTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGc                                  >  1:3084885/1‑148 (MQ=255)
                                                                                                             gTCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAAAAAGATGACGACGCACAGCTACGGCACGCCGCGGCACATTGAGAGGACCAGGCAGCACGCCTTCAGTGTTAATACCACGTTCAAAACCGTCATGCATCACTAGc                                  >  2:3117663/1‑148 (MQ=255)
                                                                                                              tCGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACAGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGcc                                 >  2:542600/1‑148 (MQ=255)
                                                                                                               cGATGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCa                                >  2:244430/1‑148 (MQ=255)
                                                                                                                  tGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGAt                             >  1:1098766/1‑148 (MQ=255)
                                                                                                                  tGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGAt                             >  1:321758/1‑148 (MQ=255)
                                                                                                                  tGACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGAt                             >  2:2513518/1‑148 (MQ=255)
                                                                                                                   gACATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATa                            <  2:340230/148‑1 (MQ=255)
                                                                                                                    aCATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATAc                           >  1:443616/1‑148 (MQ=255)
                                                                                                                    aCATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATAc                           >  1:1655894/1‑148 (MQ=255)
                                                                                                                     cATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACg                          >  2:119528/1‑148 (MQ=255)
                                                                                                                     cATTCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACg                          <  1:1977599/148‑1 (MQ=255)
                                                                                                                        tCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGc                       >  2:372752/1‑148 (MQ=255)
                                                                                                                        tCATCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGc                       >  1:2831481/1‑148 (MQ=255)
                                                                                                                          aTCGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaa                     >  2:1751041/1‑148 (MQ=255)
                                                                                                                            cGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTGATGCATCACTTGCCAGATACGGGCaaaa                   >  2:3028597/1‑148 (MQ=255)
                                                                                                                            cGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCatta                   <  2:1513133/148‑4 (MQ=255)
                                                                                                                            cGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaaa                   >  2:3028615/1‑148 (MQ=255)
                                                                                                                            cGGATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCaaaa                   <  2:1513097/148‑1 (MQ=255)
                                                                                                                              gATCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAAcc                 >  2:2097324/1‑148 (MQ=255)
                                                                                                                               aTCGTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCg                >  1:2570592/1‑148 (MQ=255)
                                                                                                                                  gTTAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCg             <  1:2141774/148‑1 (MQ=255)
                                                                                                                                    tAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTc           >  1:2158375/1‑148 (MQ=255)
                                                                                                                                    tAGAGATGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTc           >  1:493566/1‑148 (MQ=255)
                                                                                                                                      gagaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCaa         >  1:2017283/1‑148 (MQ=255)
                                                                                                                                      gagaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCaa         >  1:1808849/1‑148 (MQ=255)
                                                                                                                                        gaTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAtt       >  1:500071/1‑148 (MQ=255)
                                                                                                                                         aTGTTATAGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAAttt      >  1:1585268/1‑148 (MQ=255)
                                                                                                                                             tataGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGc  >  1:1623283/1‑148 (MQ=255)
                                                                                                                                             tataGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGc  >  1:3261142/1‑148 (MQ=255)
                                                                                                                                             tataGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGc  >  1:3143864/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAACCAGCTGACGACGCAGCGCTACGGCACGGCGCGGCACATTGAGCGGACCAGGCAGCACGCCTTCAGTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGC  >  CP009273/3252114‑3252402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑