Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,285,085 G→T 7.3% R129L (CGA→CTA)  yraK → putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,285,0850GT 7.3% 728.0 / 23.4 204R129L (CGA→CTA) yraKputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (108/81);  new base T (8/7);  total (116/88)
Fisher's exact test for biased strand distribution p-value = 7.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 204 total aligned reads displayed.

TGTCTATATGGTCAGCCCCGTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCATTTCTCGGCTCGA  >  CP009273/3284939‑3285231
                                                                                                                                                  |                                                                                                                                                  
tGTCTATATGGTCAGCCCCGTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGa                                                                                                                                                   <  2:422395/148‑1 (MQ=255)
 gTCTTTCTGGCCCGCCCCTTACTTACCACCAATGTACATAAGACTGTATATAACAAACACAATGACAGCCTGGATATTAAAACCACATTCCAGCAAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCTAt                                                                                                                                                  <  1:1509263/148‑1 (MQ=255)
    tataTGGTCAGCCCCGTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTca                                                                                                                                               <  2:501680/148‑1 (MQ=255)
       atGGTCAGCCCCGTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTcacac                                                                                                                                            >  2:1068435/1‑148 (MQ=255)
       atGGTCAGCCCCGTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTcacac                                                                                                                                            <  1:3213855/148‑1 (MQ=255)
        tGGTCAGCCCCGTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTcacaca                                                                                                                                           >  1:485284/1‑148 (MQ=255)
           tCAGCCCCGTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGAt                                                                                                                                        >  2:1743180/1‑148 (MQ=255)
               ccccGTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCTATTCACCCAGATaaaa                                                                                                                                    >  2:2903693/1‑148 (MQ=255)
               ccccGTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATaaaa                                                                                                                                    >  1:1753226/1‑148 (MQ=255)
                  cGTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAaac                                                                                                                                 >  2:1214528/1‑148 (MQ=255)
                   gTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAaaca                                                                                                                                <  1:1378013/148‑1 (MQ=255)
                   gTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAaaca                                                                                                                                >  1:2143521/1‑148 (MQ=255)
                     aCTTACCACCACTGGACATAAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAaacaac                                                                                                                              <  2:2777075/148‑1 (MQ=255)
                      cTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAacaaca                                                                                                                             <  2:2455410/148‑1 (MQ=255)
                          ccaccaCTTGACATCATACAGGATATTAAAAACTCAATGACTGCCTGTATATTAAAACCAAATTACCGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGt                                                                                                                         <  2:931483/148‑1 (MQ=255)
                          ccaccaCTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGt                                                                                                                         <  2:109488/148‑1 (MQ=255)
                            cacacTGGACATAATAAATGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGTTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGtat                                                                                                                       <  2:1998265/146‑1 (MQ=255)
                             ccacTGGACATCAGACAGTATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGtata                                                                                                                      <  2:1468917/148‑1 (MQ=255)
                             ccacTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGtata                                                                                                                      >  2:447274/1‑148 (MQ=255)
                               acTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATAtt                                                                                                                    >  2:1293875/1‑148 (MQ=255)
                                    aCATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCAAGTACTCACAACCGACAAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACTGTATATTctgct                                                                                                               >  1:363235/1‑148 (MQ=255)
                                     cATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTctgctg                                                                                                              >  1:462788/1‑148 (MQ=255)
                                        cAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCaa                                                                                                           >  2:227095/1‑148 (MQ=255)
                                          gACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAAcc                                                                                                         <  2:2325463/148‑1 (MQ=255)
                                           aCAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAAccc                                                                                                        >  1:1197402/1‑148 (MQ=255)
                                            cAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCa                                                                                                       >  1:663080/1‑148 (MQ=255)
                                             aGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCaa                                                                                                      >  1:2958627/1‑148 (MQ=255)
                                              gtatatTAAAAAATAATTGACAGCCTTTATATAAAAACCAAATTACCGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCaaa                                                                                                     <  1:3099864/146‑1 (MQ=255)
                                              ggATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCaaa                                                                                                     >  2:3099583/1‑148 (MQ=255)
                                               gATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAAc                                                                                                    >  2:2806842/1‑148 (MQ=255)
                                               gATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAAc                                                                                                    >  1:876262/1‑148 (MQ=255)
                                                  atTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACggg                                                                                                 >  1:2284066/1‑148 (MQ=255)
                                                    tACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAATAAACAACACGGTCTAGTCTGCTGCAACCCACACggggg                                                                                               >  1:1787014/1‑146 (MQ=255)
                                                    tACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGgtg                                                                                               >  2:662835/1‑148 (MQ=255)
                                                    tACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGgtg                                                                                               >  1:705831/1‑148 (MQ=255)
                                                      cAAACTCATTGACAGCCTGGATATTAAAACCACATTCCAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGtt                                                                                             <  1:2016527/148‑1 (MQ=255)
                                                      aaaaCTCAATTACAGCCTGGATATTAAAACCACATTAAAGGAAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGtt                                                                                             <  1:2539851/147‑1 (MQ=255)
                                                       aaaCTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGttt                                                                                            >  1:2203883/1‑148 (MQ=255)
                                                        aaCACAATGACACCCTGGATATTAAAACCACTTTACTGGCAAACTACATTCCCGGAAACACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTg                                                                                           <  1:1014030/148‑1 (MQ=255)
                                                         aCTCAATGACAGCCTGTATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGc                                                                                          <  1:2822734/148‑1 (MQ=255)
                                                         aCTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCTATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGc                                                                                          <  2:2011889/148‑1 (MQ=255)
                                                         aCTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGc                                                                                          <  2:978907/148‑1 (MQ=255)
                                                          ctaaaTGACAGCGTGGATATTAAACCCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGcc                                                                                         <  1:58416/145‑1 (MQ=255)
                                                              aTGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAggg                                                                                     <  2:2351424/148‑1 (MQ=255)
                                                              aTGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAggg                                                                                     <  2:2351402/148‑1 (MQ=255)
                                                               tGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGt                                                                                    <  2:823579/148‑1 (MQ=255)
                                                               tGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACACAAAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGt                                                                                    <  1:2926197/148‑1 (MQ=255)
                                                                gACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTg                                                                                   >  1:2672446/1‑148 (MQ=255)
                                                                gACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTg                                                                                   <  2:1654448/148‑1 (MQ=255)
                                                                  cAGCGTGTATATAAAAACCACATTACAGCAAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGAt                                                                                 <  1:2234934/148‑1 (MQ=255)
                                                                       tgtatatAAAAACAACATACCAGCCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGtc                                                                            <  1:1444564/145‑1 (MQ=255)
                                                                       tGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGtc                                                                            <  2:3180312/148‑1 (MQ=255)
                                                                        ggATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGtct                                                                           >  1:1147160/1‑148 (MQ=255)
                                                                        ggATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGtct                                                                           >  1:2524675/1‑148 (MQ=255)
                                                                         gATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGtctc                                                                          >  1:231000/1‑148 (MQ=255)
                                                                          atatTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCg                                                                         >  1:1494354/1‑148 (MQ=255)
                                                                          atatTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCg                                                                         >  2:3021921/1‑148 (MQ=255)
                                                                            atTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGtt                                                                       >  2:384297/1‑148 (MQ=255)
                                                                            atTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGtt                                                                       >  2:2708039/1‑148 (MQ=255)
                                                                             ttAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTa                                                                      >  2:1534801/1‑148 (MQ=255)
                                                                             ttAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTa                                                                      <  1:2321157/148‑1 (MQ=255)
                                                                              aaaaaCCACATTACAGCCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTAt                                                                     <  1:2403337/147‑1 (MQ=255)
                                                                                 aaCCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGa                                                                  >  2:1403575/1‑148 (MQ=255)
                                                                                  aCCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGAc                                                                 >  1:746159/1‑148 (MQ=255)
                                                                                   ccACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGAcc                                                                <  2:1147160/148‑1 (MQ=255)
                                                                                     acaTTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAATCAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCg                                                              >  2:361640/1‑148 (MQ=255)
                                                                                     aaaTTACAGTCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCg                                                              <  2:862923/146‑1 (MQ=255)
                                                                                        ttACAGGCAAACGACATTCCAGCACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTa                                                           <  2:2036128/148‑1 (MQ=255)
                                                                                          aCAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAat                                                         <  1:145317/148‑1 (MQ=255)
                                                                                           cAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAata                                                        >  2:1559922/1‑148 (MQ=255)
                                                                                            aGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAatat                                                       <  2:750841/148‑1 (MQ=255)
                                                                                                 aaCGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTg                                                  <  2:663080/148‑1 (MQ=255)
                                                                                                    gACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGa                                               <  1:378518/148‑1 (MQ=255)
                                                                                                      cATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCTATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAac                                             >  2:1985032/1‑148 (MQ=255)
                                                                                                      cATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAac                                             >  2:2567413/1‑148 (MQ=255)
                                                                                                         tCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTCTATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAAcacc                                          >  2:1322470/1‑148 (MQ=255)
                                                                                                         tCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTTGTGCGAAcacc                                          >  2:2021868/1‑148 (MQ=255)
                                                                                                         tCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAAcacc                                          >  2:2399315/1‑148 (MQ=255)
                                                                                                         tCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAAcacc                                          >  2:3229494/1‑148 (MQ=255)
                                                                                                           cAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAAcaccac                                        >  2:103921/1‑148 (MQ=255)
                                                                                                            aGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGAGTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACaccacc                                       <  2:685200/148‑1 (MQ=255)
                                                                                                             ggACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCt                                      >  1:2753649/1‑148 (MQ=255)
                                                                                                              gACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCtt                                     >  1:331926/1‑148 (MQ=255)
                                                                                                              gACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCtt                                     >  1:1899155/1‑148 (MQ=255)
                                                                                                              gACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCtt                                     >  1:3170545/1‑148 (MQ=255)
                                                                                                               aCTCACAACCGACCAGGTTGTCTCTGTTAACACCCTATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCttt                                    <  1:2994636/148‑1 (MQ=255)
                                                                                                                       ccGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTa                            <  1:32255/148‑1 (MQ=255)
                                                                                                                       ccGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTa                            >  2:800790/1‑148 (MQ=255)
                                                                                                                           ccAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTc                        >  1:425330/1‑148 (MQ=255)
                                                                                                                              ggTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCAcc                     >  1:1131904/1‑148 (MQ=255)
                                                                                                                              ggTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCAcc                     >  2:184894/1‑148 (MQ=255)
                                                                                                                               gTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCa                    <  1:2582424/148‑1 (MQ=255)
                                                                                                                                 tGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAg                  <  2:2958627/148‑1 (MQ=255)
                                                                                                                                   tctcTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGcc                >  1:1992029/1‑148 (MQ=255)
                                                                                                                                    ctctGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCa               >  1:1648076/1‑148 (MQ=255)
                                                                                                                                    ctctGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGAATGTCACCAAGCCa               >  1:2627398/1‑148 (MQ=255)
                                                                                                                                      ctGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCAtt             >  1:1756043/1‑148 (MQ=255)
                                                                                                                                      ctGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCAtt             >  1:3239870/1‑148 (MQ=255)
                                                                                                                                       tGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCAttt            <  2:1796126/148‑1 (MQ=255)
                                                                                                                                              acCCTATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCATTTCTCGGCt     <  2:3073076/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGTCTATATGGTCAGCCCCGTACTTACCACCACTGGACATCAGACAGGATATTACAAACTCAATGACAGCCTGGATATTAAAACCACATTACAGGCAAACGACATTCCAGGACTCACAACCGACCAGGTTGTCTCTGTTAACACCCGATTCACACAGATAAAAAACAACACGGTATATTCTGCTGCAACCCAAACGGGTGTTTGCCAGGGTGATACGTCTCGTTATGGACCCGTTAATATTGGTGCGAACACCACCTTTACCCTGTATGTCACCAAGCCATTTCTCGGCTCGA  >  CP009273/3284939‑3285231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑