Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,295,911 C→A 89.8% Q22K (CAG→AAG)  yhbV → U32 peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,295,9110CA89.8% 474.2 / 51.3 186Q22K (CAG→AAG) yhbVU32 peptidase family protein
Reads supporting (aligned to +/- strand):  ref base C (12/7);  new base A (95/72);  total (107/79)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 196 total aligned reads displayed.

GGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCACGCTG  >  CP009273/3295766‑3296054
                                                                                                                                                 |                                                                                                                                               
ggatggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATaag                                                                                                                                               <  2:712099/148‑1 (MQ=255)
 gatggaGACGCTCGGGTCGTTGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATaagc                                                                                                                                              <  1:1405949/148‑1 (MQ=255)
 gatggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAACTATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATaagc                                                                                                                                              >  2:1027890/1‑148 (MQ=255)
 gatggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATaagc                                                                                                                                              <  2:2730755/148‑1 (MQ=255)
  atggaGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATaagca                                                                                                                                             >  1:1998017/1‑148 (MQ=255)
     gagaCGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGgc                                                                                                                                          >  1:3244838/1‑148 (MQ=255)
        aCGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGgccgc                                                                                                                                       <  1:2811594/148‑1 (MQ=255)
        aCGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGgccgc                                                                                                                                       >  1:9778/1‑148 (MQ=255)
        aCGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGgccgc                                                                                                                                       >  1:10625/1‑148 (MQ=255)
          gCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGcca                                                                                                                                     >  2:26576/1‑148 (MQ=255)
               ggTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACcagc                                                                                                                                <  1:415194/148‑1 (MQ=255)
                gTCGATGTCCGACGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACcagca                                                                                                                               <  1:84/148‑1 (MQ=255)
                gTCGATGTCCGAAGCCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACcagca                                                                                                                               <  1:44/148‑1 (MQ=255)
                 tCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACcagcag                                                                                                                              >  1:1219785/1‑148 (MQ=255)
                 tCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACcagcag                                                                                                                              >  1:729238/1‑148 (MQ=255)
                   gATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAgcg                                                                                                                            <  2:2211073/148‑1 (MQ=255)
                   gATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAgcg                                                                                                                            <  2:2473004/148‑1 (MQ=255)
                    aTGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAgcgc                                                                                                                           >  1:1980140/1‑148 (MQ=255)
                    aTGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAgcgc                                                                                                                           >  1:30879/1‑148 (MQ=255)
                      gTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCg                                                                                                                         >  2:112683/1‑148 (MQ=255)
                       tCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGa                                                                                                                        >  2:1344119/1‑148 (MQ=255)
                           aaGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTg                                                                                                                    <  1:1848109/148‑1 (MQ=255)
                            aGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGa                                                                                                                   <  1:850532/148‑1 (MQ=255)
                            aGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGa                                                                                                                   <  1:3160227/148‑1 (MQ=255)
                             ggCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGAt                                                                                                                  >  2:2493191/1‑148 (MQ=255)
                                 cGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTAt                                                                                                              >  1:749314/1‑148 (MQ=255)
                                  gCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATc                                                                                                             <  2:432525/148‑1 (MQ=255)
                                   cAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCt                                                                                                            <  2:2616562/148‑1 (MQ=255)
                                     aaCCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTg                                                                                                          >  2:208575/1‑148 (MQ=255)
                                     aaCCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTg                                                                                                          >  2:1978142/1‑148 (MQ=255)
                                      aCCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCGACCAGCAGCGCCGACGTGATTTATCTTgg                                                                                                         >  2:1666002/1‑148 (MQ=255)
                                        cACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTg                                                                                                       >  2:2167209/1‑148 (MQ=255)
                                         aCTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGa                                                                                                      <  2:2706386/148‑1 (MQ=255)
                                         aCTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGa                                                                                                      <  1:700447/148‑1 (MQ=255)
                                         aCTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGa                                                                                                      <  2:2520469/148‑1 (MQ=255)
                                          ctctTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGaa                                                                                                     <  2:653779/148‑1 (MQ=255)
                                          ctctTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGaa                                                                                                     >  2:2240111/1‑148 (MQ=255)
                                           tctTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAg                                                                                                    >  1:1010649/1‑148 (MQ=255)
                                           tctTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAg                                                                                                    >  1:1010537/1‑148 (MQ=255)
                                              tGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCTCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCgg                                                                                                 >  1:618941/1‑148 (MQ=255)
                                                 cgcATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTAt                                                                                              >  1:1247178/1‑148 (MQ=255)
                                                   cATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATgc                                                                                            >  2:2978813/1‑148 (MQ=255)
                                                   cATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGTAGCGGTATgc                                                                                            >  1:2437701/1‑148 (MQ=255)
                                                   cATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATgc                                                                                            <  1:2843419/148‑1 (MQ=255)
                                                     tatCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATgcag                                                                                          <  1:227154/148‑1 (MQ=255)
                                                     tatCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATgcag                                                                                          <  2:697826/148‑1 (MQ=255)
                                                       tCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATgcagca                                                                                        <  1:1689329/148‑1 (MQ=255)
                                                           cGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCg                                                                                    >  2:1493051/1‑148 (MQ=255)
                                                            gTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGTTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGt                                                                                   <  1:116368/148‑1 (MQ=255)
                                                              aaaTGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCg                                                                                 >  1:584838/1‑148 (MQ=255)
                                                              aaaTGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCg                                                                                 >  1:2247197/1‑148 (MQ=255)
                                                                  ggCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCa                                                                             <  2:2728359/148‑1 (MQ=255)
                                                                    cAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAAc                                                                           >  2:1688595/1‑148 (MQ=255)
                                                                    cAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAAc                                                                           <  1:1921932/148‑1 (MQ=255)
                                                                       tGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCaa                                                                        <  2:2301401/148‑1 (MQ=255)
                                                                         agaAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAg                                                                      >  1:1660071/1‑148 (MQ=255)
                                                                         agaAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAg                                                                      >  2:1822430/1‑148 (MQ=255)
                                                                                cAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGa                                                               >  2:75321/1‑148 (MQ=255)
                                                                                   tGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGActg                                                            >  2:896272/1‑148 (MQ=255)
                                                                                   tGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGActg                                                            >  2:2552876/1‑148 (MQ=255)
                                                                                   tGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGAGTTATCTTGGTGAAGCGGTCTGCAGCAAGCGTCGGGCAACCAAAGTTGGCGActg                                                            >  2:2938512/1‑148 (MQ=255)
                                                                                       atatTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGActggctg                                                        >  2:2800629/1‑148 (MQ=255)
                                                                                        tatTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGActggctgg                                                       <  2:1761305/148‑1 (MQ=255)
                                                                                         atTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGga                                                      >  2:1426218/1‑148 (MQ=255)
                                                                                          ttCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGgag                                                     <  1:208575/148‑1 (MQ=255)
                                                                                             cTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTTAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATg                                                  <  2:30879/148‑1 (MQ=255)
                                                                                             cTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATg                                                  <  1:26576/148‑1 (MQ=255)
                                                                                              ttAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATgg                                                 >  1:2879388/1‑148 (MQ=255)
                                                                                               tAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGc                                                >  2:3264193/1‑148 (MQ=255)
                                                                                               tAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGc                                                >  2:1180820/1‑148 (MQ=255)
                                                                                                  ggCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCaaa                                             >  1:3272941/1‑148 (MQ=255)
                                                                                                  ggCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCaaa                                             >  2:1731196/1‑148 (MQ=255)
                                                                                                  ggCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCaaa                                             >  1:2626094/1‑148 (MQ=255)
                                                                                                  ggCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCaaa                                             <  1:1774965/148‑1 (MQ=255)
                                                                                                   gCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCaaaa                                            <  1:1086413/148‑1 (MQ=255)
                                                                                                     cAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtc                                          >  2:2009724/1‑148 (MQ=255)
                                                                                                     cAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAAtc                                          >  1:551139/1‑148 (MQ=255)
                                                                                                         gccgtgGTACTGCCCAAAATAGACGCTGGAATAATATTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATATATCTTCGTGAAGCGCTAAGCACCAATCGTCGGGCAACCAAAGTTGGAGACTGGCTGGAGAAGGAAAAAtcgctc                                      >  2:3194096/4‑148 (MQ=255)
                                                                                                              ggTACTGGCCAAAAGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCgg                                 <  1:2167209/148‑1 (MQ=255)
                                                                                                                         aaaGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGc                      >  1:2932825/1‑148 (MQ=255)
                                                                                                                         aaaGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGc                      <  1:327991/148‑1 (MQ=255)
                                                                                                                         aaaGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGc                      <  2:271587/148‑1 (MQ=255)
                                                                                                                         aaaGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGc                      <  1:502394/148‑1 (MQ=255)
                                                                                                                         aaaGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGc                      >  1:79940/1‑148 (MQ=255)
                                                                                                                          aaGAGACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCa                     >  1:2773702/1‑148 (MQ=255)
                                                                                                                           agagACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAg                    <  1:1753456/148‑1 (MQ=255)
                                                                                                                           agagACGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAACTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAg                    <  1:2653267/148‑1 (MQ=255)
                                                                                                                            gagaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGa                   >  1:2190111/1‑148 (MQ=255)
                                                                                                                             agaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGAt                  >  2:2082507/1‑148 (MQ=255)
                                                                                                                             agaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGAt                  >  2:1583107/1‑148 (MQ=255)
                                                                                                                              gaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAACTCGCTCGCCGGGAGTGGTAAGCAGAtt                 <  1:1344117/148‑1 (MQ=255)
                                                                                                                              gaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGAtt                 >  2:1922583/1‑148 (MQ=255)
                                                                                                                              gaCGCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGAtt                 <  2:2829949/148‑1 (MQ=255)
                                                                                                                                 gCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATtgtg              <  1:2009724/148‑1 (MQ=255)
                                                                                                                                 gCTGGAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATtgtg              <  1:1744955/148‑1 (MQ=255)
                                                                                                                                    ggAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGctc           >  1:1846162/1‑148 (MQ=255)
                                                                                                                                    ggAAGAATTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGctc           <  2:2468098/148‑1 (MQ=255)
                                                                                                                                     gaagaaTTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGctct          >  2:1654699/1‑148 (MQ=255)
                                                                                                                                      aagaaTTTTATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCtctc         <  2:62220/148‑1 (MQ=255)
                                                                                                                                            tttATAAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCAcgct   >  1:2688474/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAAACCACTCTTGGCGCATATCACCGTAAATGGCAGTGAGAAAAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGAATTTTATCAGCAGGCCGCCACCAGCAGCGCCGACGTGATTTATCTTGGTGAAGCGGTATGCAGCAAGCGTCGGGCAACCAAAGTTGGCGACTGGCTGGAGATGGCAAAATCGCTCGCCGGGAGTGGTAAGCAGATTGTGCTCTCCACGCTG  >  CP009273/3295766‑3296054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑