Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,306,165 C→A 35.0% V125F (GTT→TTT)  rbfA ← 30s ribosome binding factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,306,1650CA35.0% 252.5 / 129.1 156V125F (GTT→TTT) rbfA30s ribosome binding factor
Reads supporting (aligned to +/- strand):  ref base C (56/45);  new base A (28/27);  total (84/72)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

CGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAGA  >  CP009273/3306018‑3306312
                                                                                                                                                   |                                                                                                                                                   
         ttATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGttc                                                                                                                                            <  2:2443265/148‑1 (MQ=255)
             atataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCg                                                                                                                                        >  1:973779/1‑148 (MQ=255)
               ataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTc                                                                                                                                      >  1:2146821/1‑148 (MQ=255)
                taCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCa                                                                                                                                     >  1:775440/1‑148 (MQ=255)
                     ttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGttt                                                                                                                                >  1:858900/1‑148 (MQ=255)
                      tCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTg                                                                                                                               >  1:3065600/1‑148 (MQ=255)
                      tCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTg                                                                                                                               <  2:2549327/148‑1 (MQ=255)
                         cTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGAcc                                                                                                                            <  1:1330069/148‑1 (MQ=255)
                           ttttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCAc                                                                                                                          >  1:888848/1‑148 (MQ=255)
                            tttGCAGCGCATCGTTGCTGGACATACCCTGTGTTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACg                                                                                                                         <  1:119035/148‑1 (MQ=255)
                            tttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACg                                                                                                                         <  2:2028257/148‑1 (MQ=255)
                            tttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACg                                                                                                                         <  2:361379/148‑1 (MQ=255)
                            tttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACg                                                                                                                         <  1:1566354/148‑1 (MQ=255)
                             ttGCAGCGCTTCGTTGCTTGACATACCCTGCGTTTTATCAAGAAAAAAAACGCGGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGc                                                                                                                        <  1:1034390/148‑1 (MQ=255)
                               gCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCTCGCTg                                                                                                                      <  2:2583043/148‑1 (MQ=255)
                               gCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTg                                                                                                                      <  2:2582983/148‑1 (MQ=255)
                               gCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTg                                                                                                                      <  2:1821791/148‑1 (MQ=255)
                                cAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTgg                                                                                                                     >  1:1262838/1‑148 (MQ=255)
                                cAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTgg                                                                                                                     >  1:496017/1‑148 (MQ=255)
                                cAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTgg                                                                                                                     >  2:2304198/1‑148 (MQ=255)
                                cAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTgg                                                                                                                     >  1:2202097/1‑148 (MQ=255)
                                 aGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGt                                                                                                                    <  1:1016890/148‑1 (MQ=255)
                                  gcgcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTc                                                                                                                   >  2:748121/1‑148 (MQ=255)
                                   cgcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCa                                                                                                                  <  1:2998837/148‑1 (MQ=255)
                                   cgcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCa                                                                                                                  <  1:1069959/148‑1 (MQ=255)
                                    gcATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAc                                                                                                                 <  1:2158348/148‑1 (MQ=255)
                                      aTCTTTGCTGGACATACCCTGAGTTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCa                                                                                                               <  1:1693863/148‑1 (MQ=255)
                                        cGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAgg                                                                                                             >  1:627452/1‑148 (MQ=255)
                                          ttGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGtt                                                                                                           >  1:2333053/1‑148 (MQ=255)
                                           tGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGttt                                                                                                          >  1:751756/1‑148 (MQ=255)
                                           tGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGttt                                                                                                          >  1:751750/1‑148 (MQ=255)
                                            gCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTg                                                                                                         <  2:1500091/148‑1 (MQ=255)
                                                 aCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATg                                                                                                    >  1:581526/1‑148 (MQ=255)
                                                 aCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATg                                                                                                    >  1:2243902/1‑148 (MQ=255)
                                                      cccTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCAt                                                                                               >  1:1046411/1‑148 (MQ=255)
                                                           aGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCtt                                                                                          <  2:2260378/148‑1 (MQ=255)
                                                           aGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCtt                                                                                          <  1:3287375/148‑1 (MQ=255)
                                                           aGGTTTATCCAGAAACAACACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCtt                                                                                          <  1:2248893/148‑1 (MQ=255)
                                                            ggTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTc                                                                                         <  2:1361250/148‑1 (MQ=255)
                                                            ggTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTc                                                                                         <  2:1604833/148‑1 (MQ=255)
                                                              tttATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACCTGCGCATCCCTTCaa                                                                                       >  1:2865/1‑148 (MQ=255)
                                                               ttATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAc                                                                                      <  1:1343774/148‑1 (MQ=255)
                                                                tATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAAcc                                                                                     <  1:1630381/148‑1 (MQ=255)
                                                                 aTCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCa                                                                                    >  1:3259113/1‑148 (MQ=255)
                                                                   ccAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCaga                                                                                  >  2:3100416/1‑148 (MQ=255)
                                                                   ccAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATACCTTCAACCaga                                                                                  >  1:1842884/1‑148 (MQ=255)
                                                                   ccAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCaga                                                                                  >  2:1755970/1‑148 (MQ=255)
                                                                   ccAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCaga                                                                                  <  1:771629/148‑1 (MQ=255)
                                                                    cAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTAGTCATGTTTGAACACGCTGTTCACCAGGTTTGACATGCGCATCCCTTCAACCagag                                                                                 <  2:3185842/148‑1 (MQ=255)
                                                                    cAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagag                                                                                 <  2:2202097/148‑1 (MQ=255)
                                                                     aGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagaga                                                                                >  1:2923123/1‑148 (MQ=255)
                                                                     aGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagaga                                                                                >  1:1098338/1‑148 (MQ=255)
                                                                        aacaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGtt                                                                             >  2:1909436/1‑148 (MQ=255)
                                                                             aaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGt                                                                        >  2:625955/1‑148 (MQ=255)
                                                                             aaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGt                                                                        >  2:2467026/1‑148 (MQ=255)
                                                                             aaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGt                                                                        >  2:2836256/1‑148 (MQ=255)
                                                                             aaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGt                                                                        >  2:3031820/1‑148 (MQ=255)
                                                                               cGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGtagtag                                                                      >  1:2790740/1‑148 (MQ=255)
                                                                                    ttAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAgaagaa                                                                 <  1:1392792/148‑1 (MQ=255)
                                                                                     tAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGaagaag                                                                >  1:2535802/1‑148 (MQ=255)
                                                                                     tAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGaagaag                                                                <  2:2923123/148‑1 (MQ=255)
                                                                                      aaTGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAgg                                                               <  2:2617818/148‑1 (MQ=255)
                                                                                      aaTGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAgg                                                               <  2:2566084/148‑1 (MQ=255)
                                                                                       aTGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGt                                                              <  1:2704574/148‑1 (MQ=255)
                                                                                            gcgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGtt                                                         <  2:2801848/148‑1 (MQ=255)
                                                                                             cgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTc                                                        <  2:871451/148‑1 (MQ=255)
                                                                                             cgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTc                                                        <  2:1520264/148‑1 (MQ=255)
                                                                                                ccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCgg                                                     >  1:2938457/1‑148 (MQ=255)
                                                                                                   cgacgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCAc                                                  >  1:2462541/1‑148 (MQ=255)
                                                                                                   cgacgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCAc                                                  >  1:2145314/1‑148 (MQ=255)
                                                                                                   cgacgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCAc                                                  >  1:389382/1‑148 (MQ=255)
                                                                                                    gacgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACg                                                 <  2:2198153/148‑1 (MQ=255)
                                                                                                      cgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGAt                                               >  2:1968296/1‑148 (MQ=255)
                                                                                                      cgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGAt                                               >  2:1968384/1‑148 (MQ=255)
                                                                                                      cgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGAt                                               >  2:1306752/1‑148 (MQ=255)
                                                                                                      cgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGAt                                               <  2:2386627/148‑1 (MQ=255)
                                                                                                          gaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGc                                           <  2:2811528/148‑1 (MQ=255)
                                                                                                           aGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCTTGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCa                                          <  2:2094653/148‑1 (MQ=255)
                                                                                                           aGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCa                                          <  2:2094518/148‑1 (MQ=255)
                                                                                                            ggACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAg                                         <  1:2117999/148‑1 (MQ=255)
                                                                                                            ggACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCACTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAg                                         >  1:2260935/1‑148 (MQ=255)
                                                                                                                  cTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCAt                                   <  1:713844/148‑1 (MQ=255)
                                                                                                                   tCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATc                                  >  1:2495858/1‑148 (MQ=255)
                                                                                                                      ttAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCt                               >  2:3064921/1‑148 (MQ=255)
                                                                                                                       tAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCtt                              <  2:1188914/148‑1 (MQ=255)
                                                                                                                          tcctccTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTcc                           <  2:263857/148‑1 (MQ=255)
                                                                                                                          tcctccTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTcc                           >  1:1818584/1‑148 (MQ=255)
                                                                                                                           cctcctTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTccc                          <  2:284147/148‑1 (MQ=255)
                                                                                                                              cctTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCcag                       >  2:2308513/1‑148 (MQ=255)
                                                                                                                               ctTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCcagc                      <  1:955657/148‑1 (MQ=255)
                                                                                                                                ttGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCcagca                     <  1:933908/148‑1 (MQ=255)
                                                                                                                                     gtcgtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTg                >  2:519551/1‑148 (MQ=255)
                                                                                                                                      tcgtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGc               >  1:2284729/1‑148 (MQ=255)
                                                                                                                                      tcgtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGc               >  1:2284478/1‑148 (MQ=255)
                                                                                                                                      tcgtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGc               >  1:1018979/1‑148 (MQ=255)
                                                                                                                                         tcCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGa            >  2:2824137/1‑148 (MQ=255)
                                                                                                                                                tAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAAcc     >  1:2377801/1‑148 (MQ=255)
                                                                                                                                                tAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAAcc     >  1:2623831/1‑148 (MQ=255)
                                                                                                                                                   cacGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCACCCAGAGAGTTGTCGTCGAAGAAGGTCAGTTCCGGCACGATACGCCGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAATCCAGa  >  2:2983004/1‑148 (MQ=255)
                                                                                                                                                   cacGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAGa  >  2:1608776/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGGTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAGA  >  CP009273/3306018‑3306312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑