Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,312,862 T→A 8.1% R289R (CGT→CGA)  argG → argininosuccinate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,312,8620TA 8.1% 635.0 / 37.5 210R289R (CGT→CGA) argGargininosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base T (102/91);  new base A (7/10);  total (109/101)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 210 total aligned reads displayed.

TTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTT  >  CP009273/3312716‑3313005
                                                                                                                                                  |                                                                                                                                               
tttGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTg                                                                                                                                                >  2:3050945/1‑148 (MQ=255)
 ttGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTgg                                                                                                                                               <  2:261698/148‑1 (MQ=255)
    aaCAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTAt                                                                                                                                            >  1:1385923/1‑148 (MQ=255)
    aaCAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTAt                                                                                                                                            >  2:2163214/1‑148 (MQ=255)
      cAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTAttt                                                                                                                                          <  2:1549145/148‑1 (MQ=255)
       aaGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTa                                                                                                                                         <  2:2201250/148‑1 (MQ=255)
           tCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGaa                                                                                                                                     >  2:1280847/1‑148 (MQ=255)
                  gTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCgg                                                                                                                              <  1:3264220/148‑1 (MQ=255)
                      cgcTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGAt                                                                                                                          <  2:51089/148‑1 (MQ=255)
                        cTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATgg                                                                                                                        >  2:3143349/1‑148 (MQ=255)
                          gAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCa                                                                                                                      >  2:1103749/1‑148 (MQ=255)
                          gAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCa                                                                                                                      <  1:1609627/148‑1 (MQ=255)
                          gAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCa                                                                                                                      >  1:493538/1‑148 (MQ=255)
                           aaCGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCAc                                                                                                                     >  1:3153664/1‑148 (MQ=255)
                           aaCGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCAc                                                                                                                     >  1:992833/1‑148 (MQ=255)
                           aaCGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCAc                                                                                                                     >  1:2929704/1‑148 (MQ=255)
                           aaCGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCAc                                                                                                                     >  1:3140175/1‑148 (MQ=255)
                             cGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCActg                                                                                                                   >  2:666431/1‑148 (MQ=255)
                                  aaaCCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGca                                                                                                              >  1:871819/1‑148 (MQ=255)
                                   aaCCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGAGGTATTTACGAAGCTCCGGGGATGGCACTGCTGcac                                                                                                             >  1:26267/1‑148 (MQ=255)
                                    aCCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGcaca                                                                                                            >  2:2758850/1‑148 (MQ=255)
                                    aCCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGcaca                                                                                                            <  1:1249248/148‑1 (MQ=255)
                                        ttAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGc                                                                                                        >  1:613116/1‑148 (MQ=255)
                                        ttAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGc                                                                                                        <  1:738821/148‑1 (MQ=255)
                                           gCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTa                                                                                                     <  1:1740484/148‑1 (MQ=255)
                                           gCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTa                                                                                                     <  1:1242982/148‑1 (MQ=255)
                                           gCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTa                                                                                                     <  1:838907/148‑1 (MQ=255)
                                           gCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTa                                                                                                     >  1:1622433/1‑148 (MQ=255)
                                           gCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTa                                                                                                     <  1:1684735/148‑1 (MQ=255)
                                           gCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTa                                                                                                     >  1:2263603/1‑148 (MQ=255)
                                           gCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGAGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTa                                                                                                     <  1:1284739/148‑1 (MQ=255)
                                            cgacgaCGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTAt                                                                                                    >  2:3134164/1‑148 (MQ=255)
                                            cgacgaCGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTAt                                                                                                    >  2:1810094/1‑148 (MQ=255)
                                               cgacgTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGaa                                                                                                 <  1:1452904/148‑1 (MQ=255)
                                                 acgTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACg                                                                                               <  1:1103749/148‑1 (MQ=255)
                                                  cgTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGc                                                                                              <  1:3063093/148‑1 (MQ=255)
                                                  cgTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGc                                                                                              <  2:619583/148‑1 (MQ=255)
                                                    tttaaaTGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCTACCAGATTGAAAACCGTATCATCGAAGCGAACAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTTCGTATGAACGCCt                                                                                            <  2:315073/145‑1 (MQ=255)
                                                    tAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCt                                                                                            >  2:2149305/1‑148 (MQ=255)
                                                      gAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGt                                                                                          <  1:2198591/148‑1 (MQ=255)
                                                       aaaTGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGtt                                                                                         >  2:290159/1‑148 (MQ=255)
                                                       aaaTGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGtt                                                                                         >  1:2908693/1‑148 (MQ=255)
                                                        aaTGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGGATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGAACATTGCGTATGTACGCCTGTTg                                                                                        <  2:2293507/148‑1 (MQ=255)
                                                        aaTGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTg                                                                                        <  1:2890079/148‑1 (MQ=255)
                                                        aaTGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTg                                                                                        >  2:315512/1‑148 (MQ=255)
                                                         atgatgCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGa                                                                                       <  2:483976/148‑1 (MQ=255)
                                                         atgatgCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGa                                                                                       >  2:2331542/1‑148 (MQ=255)
                                                          tgatgCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGAc                                                                                      >  2:216034/1‑148 (MQ=255)
                                                           gatgCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGAGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGAcc                                                                                     >  1:687959/1‑148 (MQ=255)
                                                              gCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGt                                                                                  <  1:2330084/148‑1 (MQ=255)
                                                                 ggAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTAtt                                                                               <  1:3050945/148‑1 (MQ=255)
                                                                  gAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTc                                                                              >  2:2124891/1‑148 (MQ=255)
                                                                   aaGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTca                                                                             >  1:2541453/1‑148 (MQ=255)
                                                                   aaGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTca                                                                             >  1:886331/1‑148 (MQ=255)
                                                                   aaGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTca                                                                             <  2:336675/148‑1 (MQ=255)
                                                                    aGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTcac                                                                            <  2:1484347/148‑1 (MQ=255)
                                                                     gCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTcaca                                                                           >  2:301547/1‑148 (MQ=255)
                                                                         aCCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGa                                                                       >  2:485206/1‑148 (MQ=255)
                                                                         aCCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGa                                                                       >  2:1936572/1‑148 (MQ=255)
                                                                         aCCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGAGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGa                                                                       >  2:803564/1‑148 (MQ=255)
                                                                          ccGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGaa                                                                      <  2:2085272/148‑1 (MQ=255)
                                                                          ccGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGaa                                                                      >  2:2586707/1‑148 (MQ=255)
                                                                          ccGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGaa                                                                      >  2:2586589/1‑148 (MQ=255)
                                                                            gCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGa                                                                    <  2:1385923/148‑1 (MQ=255)
                                                                             cATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGac                                                                   >  2:2736371/1‑148 (MQ=255)
                                                                                cggcggTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACAcc                                                                <  1:365707/148‑1 (MQ=255)
                                                                                 ggcggTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCa                                                               <  1:212988/148‑1 (MQ=255)
                                                                                      tcgtcACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAg                                                          <  1:2823015/148‑1 (MQ=255)
                                                                                         tcACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAg                                                       >  1:1847185/1‑148 (MQ=255)
                                                                                          cACGGCCTGGGCATGAGCGACCAGATTGAAAACCGAATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGATCAGt                                                      >  2:302306/1‑148 (MQ=255)
                                                                                             ggCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATc                                                   <  2:3228558/148‑1 (MQ=255)
                                                                                             ggCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGAGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATc                                                   <  1:1193023/148‑1 (MQ=255)
                                                                                             ggCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGAGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATc                                                   <  1:161867/148‑1 (MQ=255)
                                                                                                cTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCAcg                                                >  1:3254842/1‑148 (MQ=255)
                                                                                                cTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCAcg                                                <  1:499328/148‑1 (MQ=255)
                                                                                                      aTGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATg                                          >  2:1923392/1‑148 (MQ=255)
                                                                                                        gAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGgt                                        >  2:209906/1‑148 (MQ=255)
                                                                                                           cGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGgtcgt                                     <  2:360401/148‑1 (MQ=255)
                                                                                                           cGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGgtcgt                                     >  1:635851/1‑148 (MQ=255)
                                                                                                             aCCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGAGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCa                                   <  2:1963077/148‑1 (MQ=255)
                                                                                                              ccAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAg                                  >  1:1791511/1‑148 (MQ=255)
                                                                                                              ccAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAg                                  <  2:1415174/148‑1 (MQ=255)
                                                                                                               cAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGt                                 <  1:2758834/148‑1 (MQ=255)
                                                                                                                    tGAAAACCTTCTCATCGAAGCGAATTGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGTACGGCTGTTGTCCGTTATTCACAACGAAGACAGCATTGAGCAGTATCACGCGCATGGTCGTCAGTTggga                            <  2:2943215/148‑2 (MQ=255)
                                                                                                                    tGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGc                            >  2:1056510/1‑148 (MQ=255)
                                                                                                                       aaaCCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGAATCACGCGCATGGTCGTCAGTTGGGCCGt                         >  1:1007915/1‑148 (MQ=255)
                                                                                                                         aCCGTCTCATCGAAGCGAAAAGCCGTGGAAGTTACGAAGCTCCGGGGATGGCACGGCTGCACATTGCGTATGAACGCCTGTTGACCGGTCTTCACAACGACGACACCAGTGAGCAGTCTCACGCGCCTGGGCGTCAGGTGGGCCGTct                       >  2:2998504/1‑148 (MQ=255)
                                                                                                                         aCCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTct                       >  2:3082280/1‑148 (MQ=255)
                                                                                                                         aCCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTct                       >  1:1527950/1‑148 (MQ=255)
                                                                                                                         aCCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTct                       >  2:3226096/1‑148 (MQ=255)
                                                                                                                           cGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTctgc                     <  2:2546593/148‑1 (MQ=255)
                                                                                                                               tcatcGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTa                 >  2:2107602/1‑148 (MQ=255)
                                                                                                                               tcatcGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTa                 >  2:2645573/1‑148 (MQ=255)
                                                                                                                                catcGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTAc                <  2:978982/148‑1 (MQ=255)
                                                                                                                                  tcGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCa              >  2:2254464/1‑148 (MQ=255)
                                                                                                                                        cGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCg        <  1:202402/148‑1 (MQ=255)
                                                                                                                                         gAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGt       <  1:2388412/148‑1 (MQ=255)
                                                                                                                                           aaaGCCGAGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTg     >  1:2387293/1‑148 (MQ=255)
                                                                                                                                            aaGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTgg    <  2:3304952/148‑1 (MQ=255)
                                                                                                                                              gCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGtt  <  1:3134188/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TTTGAACAAGGTCATCCGGTGGCGCTGAACGGTAAAACCTTTAGCGACGACGTAGAAATGATGCTGGAAGCTAACCGCATCGGCGGTCGTCACGGCCTGGGCATGAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTT  >  CP009273/3312716‑3313005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑