Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,390,573 G→T 6.8% T194T (ACC→ACA)  rng ← ribonuclease G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,390,5730GT 6.8% 609.7 / 2.7 186T194T (ACC→ACA) rngribonuclease G
Reads supporting (aligned to +/- strand):  ref base G (66/101);  new base T (17/2);  total (83/103)
Fisher's exact test for biased strand distribution p-value = 3.33e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

AGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCA  >  CP009273/3390427‑3390720
                                                                                                                                                  |                                                                                                                                                   
aGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTGTTTTACGCTCCCTAACTGTgg                                                                                                                                                    >  2:3057708/1‑148 (MQ=255)
 gTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGt                                                                                                                                                   <  1:1159164/148‑1 (MQ=255)
    aCGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTc                                                                                                                                                  <  1:945176/146‑1 (MQ=255)
    aCGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTc                                                                                                                                                  >  2:945154/1‑146 (MQ=255)
          cGTAAGTCAGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGAcgcg                                                                                                                                          <  2:1283405/148‑1 (MQ=255)
           gTAAGTCAGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGt                                                                                                                                         <  1:2101217/148‑1 (MQ=255)
               gTCAGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCa                                                                                                                                     >  2:1932630/1‑148 (MQ=255)
                tCAGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAg                                                                                                                                    >  2:2556418/1‑148 (MQ=255)
                tCAGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTGTTTTAAGCTCCCAAACTTTGGTGCCGACGCGTTTCAg                                                                                                                                    >  1:2597511/1‑148 (MQ=255)
                  aGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGAt                                                                                                                                  <  1:2044013/148‑1 (MQ=255)
                     cGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAg                                                                                                                               <  2:2696954/148‑1 (MQ=255)
                     cGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTGTTTTTAGCTCCATAACATTGGTCCAGACGCGGTTCAGATAAg                                                                                                                               >  1:708351/1‑148 (MQ=255)
                          gTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCa                                                                                                                          <  1:2145076/148‑1 (MQ=255)
                            cAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTGTTTTAAGCTCCCAAAATTTTGTGCCGACGCGTTTGAGATAAGAGGCATc                                                                                                                        >  1:1232529/1‑148 (MQ=255)
                              aCGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCgg                                                                                                                      >  2:1765033/1‑148 (MQ=255)
                                 cgAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGTGTCTGCGGGCGTTTTTTTAGCTCCAAAACCTTTGTGCATTCGCGTTTACTTTAAACGGCATAGGatg                                                                                                                   >  1:593528/1‑146 (MQ=255)
                                 cgAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAgg                                                                                                                   <  2:1885667/148‑1 (MQ=255)
                                    aTGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCa                                                                                                                <  1:2176701/148‑1 (MQ=255)
                                     tGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTTGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAg                                                                                                               <  2:281563/148‑1 (MQ=255)
                                     tGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTTGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAg                                                                                                               <  2:282088/148‑1 (MQ=255)
                                           ccAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTTGTCCAGACGCGGTTCAGATAAGCGGCATCGGCGGCCCGTTCAGc                                                                                                         >  2:379211/1‑148 (MQ=255)
                                           ccAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGc                                                                                                         <  2:481566/148‑1 (MQ=255)
                                           ccAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGc                                                                                                         <  2:2368298/148‑1 (MQ=255)
                                            cAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGcc                                                                                                        <  1:336353/148‑1 (MQ=255)
                                               ttCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCg                                                                                                     >  2:1043594/1‑148 (MQ=255)
                                               ttCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCg                                                                                                     >  2:1556316/1‑148 (MQ=255)
                                               ttCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTGTTTTAAGCTCCAAAAAATTTGTCCACACGCGGTTCAAATAATACGCATCGGAGGCCACCTCAGACTcc                                                                                                     >  1:2903494/1‑147 (MQ=255)
                                                    cATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACGTTGGTCCCGACGCGGTTCAGATAAGCGGCATCGGAGGCCCGTTCCGCCGCGCCAAc                                                                                                >  1:1912006/1‑148 (MQ=255)
                                                    cATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTAAGCGCCAAAACTTTGGTCCAGACCCGTTTCAGATAAGCGGCATCGGAGGCCCCTTCAGCCTCGCCCAc                                                                                                >  1:2056223/1‑148 (MQ=255)
                                                     aTCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTAAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAAcc                                                                                               >  2:1080876/1‑148 (MQ=255)
                                                      tCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAAccc                                                                                              <  1:2622599/148‑1 (MQ=255)
                                                      tCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTAAGCTCCAAAACGTTTGTGCAGGCGCGGTTGAGATAAACGGCATCGGGGGCCAGTTAAGCCTCGCCAAccc                                                                                              >  1:1330582/1‑148 (MQ=255)
                                                         gCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCtt                                                                                           <  1:2280372/148‑1 (MQ=255)
                                                            aaaTCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCCTCGGAGGCCAGTTCAGCCTCGCCAACCCCATCCg                                                                                        >  2:3110290/1‑148 (MQ=255)
                                                            aaaTCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCg                                                                                        <  2:2955225/148‑1 (MQ=255)
                                                              aTCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCt                                                                                      >  2:788815/1‑148 (MQ=255)
                                                                cACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTGTTTTACGCTCCATAACTTTGGTCCAGACGCGGTTAAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCGTACGCTGc                                                                                    >  2:2229953/1‑148 (MQ=255)
                                                                   gCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGt                                                                                 <  2:2867517/148‑1 (MQ=255)
                                                                    cAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTAAGGTCCCAAACTTTGGTGCAGACGCGGTTCAGATAAACGGCCTCGGAGGCCCGTTAACCCTCGCCAACCCCATCCCCAGCgggc                                                                                >  1:978473/1‑146 (MQ=255)
                                                                    cAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTGTTTTTTGGGCCCAAACTTTTGTGCAGACGGGGTTGAGATAATCCGCCACGGGGGGCCGTTTAGCCACGCCCCCCCCGGCCGCGGCGGtg                                                                                >  1:1432591/1‑147 (MQ=255)
                                                                       aaCACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACgg                                                                             <  2:2756140/148‑1 (MQ=255)
                                                                       aaCACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTAAGCTCCATAAATTTTGTCCAGACGCGGTTGAGATAAACGGCCTCGGAGGCCAGTTCAGCCTCGCCCACCCCATCCCCTGCGGTACgg                                                                             >  1:1010281/1‑148 (MQ=255)
                                                                       aaCACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTAAGCTCCATAAAATTTGTCCCGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTGAGCCGCGCCCACCCCCTCCGCTGCggggcgg                                                                             >  1:145262/1‑143 (MQ=255)
                                                                         cacGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTGCAGACGCGTTTCAGATAATCGGCCTCGGAGGCCCGTTCAGCCGCGCCCACCCCATCCGCTGCGGTACGgat                                                                           >  1:3189091/1‑148 (MQ=255)
                                                                         cacGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGgat                                                                           <  1:532626/148‑1 (MQ=255)
                                                                              tGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATaa                                                                      <  2:3085884/148‑1 (MQ=255)
                                                                                cgccagcgccagTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAAc                                                                    <  1:2241750/148‑1 (MQ=255)
                                                                                cgccagcgccagTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAAc                                                                    <  2:3274451/148‑1 (MQ=255)
                                                                                cgccagcgccagGTCGCCGTACAGCTGATCACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAAc                                                                    <  2:1739324/148‑1 (MQ=255)
                                                                                    agcgccagTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGc                                                                <  1:1040464/148‑1 (MQ=255)
                                                                                     gcgccagTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGcc                                                               <  1:939421/148‑1 (MQ=255)
                                                                                     gcgccagTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTACAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGcc                                                               >  2:253694/1‑148 (MQ=255)
                                                                                     gcgccagTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCACCATAACATTTGTAAAGACGCGTTTAAGATAAGCGGCAACGGAGGCCAGTTCAGACTCGCCAACACCATCCGCTGCGGTACGGATGTTAAAcacccc                                                               >  2:165907/1‑143 (MQ=255)
                                                                                      cgccagTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGccc                                                              <  1:1807186/148‑1 (MQ=255)
                                                                                         cagTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGc                                                           <  1:1498740/148‑1 (MQ=255)
                                                                                         cagTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGc                                                           <  1:1716623/148‑1 (MQ=255)
                                                                                            ttCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCCGACGCGGTTCAGATAAGCGGCATCGGAGGCCAGGTCAGCCTCGCCAACCCCGTCCGCTGCGGTACGGTTGATAAACCCGCCCTGCtcg                                                        >  2:1268394/1‑148 (MQ=255)
                                                                                                 cGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCa                                                   <  2:2519531/148‑1 (MQ=255)
                                                                                                   tACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTACAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAAt                                                 <  1:253700/148‑1 (MQ=255)
                                                                                                   tACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAAATTTGGTACAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAAt                                                 <  1:165907/148‑1 (MQ=255)
                                                                                                    aCAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATa                                                <  1:2604298/148‑1 (MQ=255)
                                                                                                    aCAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATa                                                <  2:2330571/148‑1 (MQ=255)
                                                                                                    aCAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATa                                                <  2:1965788/148‑1 (MQ=255)
                                                                                                      aGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATAct                                              <  2:282575/148‑1 (MQ=255)
                                                                                                      aGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATAct                                              <  1:734497/148‑1 (MQ=255)
                                                                                                        cTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATActct                                            >  2:1836185/1‑148 (MQ=255)
                                                                                                        cTGATAACGGGTCTGCGGGCGTTTTTTAAGCTCCATAACATTGGTGCAGACGCGGTTCAGATAAGCGGCATCGGAGGCCCGGTCAGCCTCGCCAACCCCGTCCGCTGCGGTACGGATTATAAACCCCCCCAGCTCGTCGCAAAAcact                                            >  1:2708954/1‑148 (MQ=255)
                                                                                                        cTGATAACGGGTCTGCGGGCGTTTTTTAAGCTCCAAAACATTTGTGCAGACGCGTTTCAGATAAGCCGCAACGGAGGCCAGTTCATCCGCGCCAACCCCATCCGCAGCGGGACGGGTGATAAAACCCCCCTGCTCGTCGCAATActat                                            >  1:1056215/1‑146 (MQ=255)
                                                                                                               cGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCa                                     <  1:409849/148‑1 (MQ=255)
                                                                                                                 ggTCTGCGGGCGTTTTTTACCCTCCATAAAATTGGTCCACACGCCTTTCAGATAATCGGCCTAGGAGGCCAGTTCAGCCACGCCCACCCCATACCCGGCGGTACGGATGATAAAACCCCCCAGCGCCCCGCAAAACTCTGCGACCAca                                   >  2:859074/1‑147 (MQ=255)
                                                                                                                     tGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCATCCGCTGCGGTACGGATGATAAACCCGCCCAGCTCGTCGCAAAACTCTGCGACCACatttt                               >  2:2607993/1‑148 (MQ=255)
                                                                                                                     tGCGGGCGTTTTTTACGCTCCATAAATTTTGTACAGACGCGTTTAATATAATCGGCATCGGAGGCCAGTTAAGCCTCGCCCACCCCATCCGCAGCGGTAAGGCTGATAAACCCCCCCAGCTCGACGCAATAATCAGCGACCACttttt                               >  2:721729/1‑148 (MQ=255)
                                                                                                                      gCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACtttttt                              <  1:817261/148‑1 (MQ=255)
                                                                                                                      gCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACtttttt                              <  1:366581/148‑1 (MQ=255)
                                                                                                                       cGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTc                             <  1:3044270/148‑1 (MQ=255)
                                                                                                                         ggCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAg                           <  1:2052775/148‑1 (MQ=255)
                                                                                                                                  tACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACg                  <  2:888779/148‑1 (MQ=255)
                                                                                                                                   aCGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCTCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGACCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGt                 <  2:140727/148‑1 (MQ=255)
                                                                                                                                   aCGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGt                 <  2:140724/148‑1 (MQ=255)
                                                                                                                                    cGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGtt                <  1:939505/148‑1 (MQ=255)
                                                                                                                                     gCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTc               <  1:3149888/148‑1 (MQ=255)
                                                                                                                                     gCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTc               >  1:136947/1‑148 (MQ=255)
                                                                                                                                     gCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAAACCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTc               >  1:1634281/1‑148 (MQ=255)
                                                                                                                                       tCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTg             <  1:1491409/148‑1 (MQ=255)
                                                                                                                                       tCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTg             >  1:2731782/1‑148 (MQ=255)
                                                                                                                                       tCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTg             <  1:2479392/148‑1 (MQ=255)
                                                                                                                                         cATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGAt           <  2:1330582/148‑1 (MQ=255)
                                                                                                                                           tAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTc         >  2:2076090/1‑148 (MQ=255)
                                                                                                                                            aaCTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCg        >  2:2683703/1‑148 (MQ=255)
                                                                                                                                            aaCTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCg        <  1:3242034/148‑1 (MQ=255)
                                                                                                                                             aCTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGc       >  1:2855417/1‑148 (MQ=255)
                                                                                                                                             aCTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGc       >  1:3138056/1‑148 (MQ=255)
                                                                                                                                             aCTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTGCTCGTTCTGATTCGc       >  1:2371119/1‑148 (MQ=255)
                                                                                                                                               tttGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCtt     <  1:847176/148‑1 (MQ=255)
                                                                                                                                               tttGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCtt     >  2:1788202/1‑148 (MQ=255)
                                                                                                                                                ttGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCttt    <  2:3200506/148‑1 (MQ=255)
                                                                                                                                                ttGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCttt    >  1:1556970/1‑148 (MQ=255)
                                                                                                                                                ttGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCttt    >  1:1557029/1‑148 (MQ=255)
                                                                                                                                                ttGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCttt    >  1:3262119/1‑148 (MQ=255)
                                                                                                                                                ttGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCttt    >  1:1958219/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGTAACGCTTCGTAAGTCAGGCGTGAGTCAACGCGAATGCGGTCCAGTTCGGCATCGGCGAAATCACGCAGAACACGCTGCGCCAGCGCCAGTTCGCCGTACAGCTGATAACGGGTCTGCGGGCGTTTTTTACGCTCCATAACTTTGGTCCAGACGCGTTTCAGATAAGCGGCATCGGAGGCCAGTTCAGCCTCGCCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCA  >  CP009273/3390427‑3390720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑