Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,396,082 G→T 100% R204S (CGT→AGT)  csrD ← targeting factor for csrBC sRNA degradation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,396,0820GT100.0% 628.3 / NA 196R204S (CGT→AGT) csrDtargeting factor for csrBC sRNA degradation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (101/95);  total (101/95)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 196 total aligned reads displayed.

ACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCG  >  CP009273/3395937‑3396226
                                                                                                                                                 |                                                                                                                                                
aCTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTaa                                                                                                                                                >  1:1783809/1‑148 (MQ=255)
 cTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGGGATTGAGGCCGGTTTTCACGTCCGGGGCGGCATAAGAGCGGCTCAGCGGATCAAGACGGCTGTGTTGTTAGCGGGCGGTCTGAATTTCACTAag                                                                                                                                               >  1:340425/1‑148 (MQ=255)
    tctcCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAagcag                                                                                                                                            <  1:2806275/148‑1 (MQ=255)
       ccTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGt                                                                                                                                         <  1:1331456/148‑1 (MQ=255)
       ccTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGt                                                                                                                                         <  2:240162/148‑1 (MQ=255)
         tGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTAt                                                                                                                                       >  1:536594/1‑148 (MQ=255)
         tGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTAt                                                                                                                                       >  2:2209972/1‑148 (MQ=255)
         tGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGGTATTGCGGCCGGTTTTCAAGTCCTGGGCGGGCTAAGAGCGGATCAGCGTATCAATACGGCTGTGTTTTTCGCGTGCGTTCTGTATTTCACTAAGCAGCCTAt                                                                                                                                       >  1:1964482/1‑148 (MQ=255)
           aTCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAATACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATcc                                                                                                                                     >  1:2665643/1‑148 (MQ=255)
           aTCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATcc                                                                                                                                     <  2:1566036/148‑1 (MQ=255)
           aTCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGGTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATcc                                                                                                                                     >  1:516366/1‑148 (MQ=255)
                    tAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTAGCGTGCGGTCTGAATTTCACTAAGCCGCGTATCCAGCGCActg                                                                                                                            >  2:2519422/1‑148 (MQ=255)
                     aaCGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCActgc                                                                                                                           >  2:2207943/1‑148 (MQ=255)
                      aCGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTAGCGTGCGGTCTGAATTTCACTAAGCAGCGTATCCAGCGCActgct                                                                                                                          >  1:2383302/1‑148 (MQ=255)
                           gCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTc                                                                                                                     <  1:962935/148‑1 (MQ=255)
                           gCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTc                                                                                                                     <  2:2743694/148‑1 (MQ=255)
                           gCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTc                                                                                                                     <  2:2821868/148‑1 (MQ=255)
                            cTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCt                                                                                                                    <  2:2555309/148‑1 (MQ=255)
                            cTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCt                                                                                                                    >  2:2993016/1‑148 (MQ=255)
                               aCTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTggg                                                                                                                 >  2:2544419/1‑148 (MQ=255)
                               aCTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTggg                                                                                                                 <  2:2600461/148‑1 (MQ=255)
                                   aTTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGggcggc                                                                                                             >  2:1471622/1‑148 (MQ=255)
                                     tATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCa                                                                                                           <  1:1033499/148‑1 (MQ=255)
                                      aTCGAAAAAGAGTCGGTTATTTAGGCCCGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATAAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCAt                                                                                                          >  1:1751638/1‑148 (MQ=255)
                                            aaaGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAg                                                                                                    >  1:2996987/1‑148 (MQ=255)
                                                   cGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTc                                                                                             >  2:190416/1‑148 (MQ=255)
                                                   cGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTc                                                                                             <  2:3228554/148‑1 (MQ=255)
                                                    ggTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTcc                                                                                            <  1:1237689/148‑1 (MQ=255)
                                                    ggTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTcc                                                                                            <  1:1237436/148‑1 (MQ=255)
                                                    ggTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTcc                                                                                            <  1:3038506/148‑1 (MQ=255)
                                                     gTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTccc                                                                                           >  2:766206/1‑148 (MQ=255)
                                                      ttATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCa                                                                                          >  2:1493674/1‑148 (MQ=255)
                                                      ttATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGAGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGtt                                                                                                                      <  2:1340054/120‑1 (MQ=255)
                                                           gAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAAcaca                                                                                     <  1:72242/148‑1 (MQ=255)
                                                           gAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAAcaca                                                                                     <  1:71217/148‑1 (MQ=255)
                                                           gAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAAcaca                                                                                     <  1:1745956/148‑1 (MQ=255)
                                                           gAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAAcaca                                                                                     <  1:11422/148‑1 (MQ=255)
                                                           gAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAAcaca                                                                                     <  1:11943/148‑1 (MQ=255)
                                                            aGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACAt                                                                                    >  1:406964/1‑148 (MQ=255)
                                                             ggCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACAtt                                                                                   <  2:516366/148‑1 (MQ=255)
                                                             ggCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACAtt                                                                                   >  1:1625747/1‑148 (MQ=255)
                                                              gCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTa                                                                                  <  1:3168118/148‑1 (MQ=255)
                                                                cGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTaga                                                                                <  2:2076600/148‑1 (MQ=255)
                                                                 ggTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTagag                                                                               <  1:2992653/148‑1 (MQ=255)
                                                                 ggTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTagag                                                                               <  1:2026676/148‑1 (MQ=255)
                                                                   ttttCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGcc                                                                             >  2:689213/1‑148 (MQ=255)
                                                                   ttttCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGcc                                                                             >  2:822964/1‑148 (MQ=255)
                                                                       cACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGc                                                                         >  2:1092001/1‑148 (MQ=255)
                                                                           tCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCAc                                                                     >  2:1158202/1‑148 (MQ=255)
                                                                            ccTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCAcc                                                                    >  2:88940/1‑148 (MQ=255)
                                                                              tGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGt                                                                  <  1:1013124/148‑1 (MQ=255)
                                                                                 gcggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTa                                                               <  1:3019886/148‑1 (MQ=255)
                                                                                    gcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGa                                                            >  2:29148/1‑148 (MQ=255)
                                                                                      aTAAGATCTGATCATCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCTGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATa                                                          <  1:3240173/148‑1 (MQ=255)
                                                                                      aTAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATa                                                          <  1:3115170/148‑1 (MQ=255)
                                                                                        aaGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACg                                                        >  1:1425480/1‑148 (MQ=255)
                                                                                        aaGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACg                                                        <  2:2195775/148‑1 (MQ=255)
                                                                                         agagCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGa                                                       <  1:2540476/148‑1 (MQ=255)
                                                                                          gagCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAg                                                      >  1:2375901/1‑148 (MQ=255)
                                                                                          gagCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAg                                                      <  2:1854377/148‑1 (MQ=255)
                                                                                               gATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGcc                                                 >  2:3107425/1‑148 (MQ=255)
                                                                                                 tCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCg                                               <  2:568003/148‑1 (MQ=255)
                                                                                                   aGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCggg                                             >  1:3288358/1‑148 (MQ=255)
                                                                                                      gTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGttt                                          <  1:3216088/148‑1 (MQ=255)
                                                                                                       tATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTc                                         <  2:106771/148‑1 (MQ=255)
                                                                                                        aTCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTcc                                        <  1:623909/148‑1 (MQ=255)
                                                                                                        aTCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTcc                                        <  2:2104038/148‑1 (MQ=255)
                                                                                                          cAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAg                                      >  1:179170/1‑148 (MQ=255)
                                                                                                            aGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCa                                    >  1:2147852/1‑148 (MQ=255)
                                                                                                            aGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCa                                    >  2:2736731/1‑148 (MQ=255)
                                                                                                             gACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCaa                                   >  1:2641193/1‑148 (MQ=255)
                                                                                                              aCGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAAt                                  >  2:674151/1‑148 (MQ=255)
                                                                                                              aCGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAAt                                  >  2:2744024/1‑148 (MQ=255)
                                                                                                              aCGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAAt                                  >  2:1062726/1‑148 (MQ=255)
                                                                                                               cGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAAtt                                 <  1:3036307/148‑1 (MQ=255)
                                                                                                                ggCTGTGTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTc                                <  1:1324483/148‑1 (MQ=255)
                                                                                                                   tgtgTTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTg                             <  1:29148/148‑1 (MQ=255)
                                                                                                                    gtgtTGTTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTCGCCCGGGTTTCCAGCAATTCTTGc                            >  2:3055264/1‑148 (MQ=255)
                                                                                                                     tgttgtTCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGcc                           >  1:900535/1‑148 (MQ=255)
                                                                                                                      gttgttCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGccc                          <  1:1608374/148‑1 (MQ=255)
                                                                                                                        tgttCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCgg                        >  1:1996682/1‑148 (MQ=255)
                                                                                                                          ttCGCGTGCGTTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCa                      >  2:174283/1‑148 (MQ=255)
                                                                                                                           tCGCGTGCGGTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCaa                     >  1:721439/1‑148 (MQ=255)
                                                                                                                             gcgtgcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGt                   <  2:2942409/148‑1 (MQ=255)
                                                                                                                                tgcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGc                >  1:1270999/1‑148 (MQ=255)
                                                                                                                                tgcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGc                >  1:125669/1‑148 (MQ=255)
                                                                                                                                 gcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGcc               >  1:1508918/1‑148 (MQ=255)
                                                                                                                                 gcgtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGcc               >  2:1377315/1‑148 (MQ=255)
                                                                                                                                   gtTCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGt             <  2:1288523/148‑1 (MQ=255)
                                                                                                                                    ttCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGtt            <  1:941652/148‑1 (MQ=255)
                                                                                                                                     tCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTg           >  1:1720650/1‑148 (MQ=255)
                                                                                                                                     tCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTg           <  2:1889713/148‑1 (MQ=255)
                                                                                                                                     tCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTg           >  1:3069925/1‑148 (MQ=255)
                                                                                                                                     tCTGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTg           >  1:2184329/1‑148 (MQ=255)
                                                                                                                                       tGAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTa         >  1:219892/1‑148 (MQ=255)
                                                                                                                                        gAATTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTaa        >  1:107973/1‑148 (MQ=255)
                                                                                                                                         aaTTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAc       >  1:1812788/1‑148 (MQ=255)
                                                                                                                                         aaTTTCACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAAc       <  2:1783809/148‑1 (MQ=255)
                                                                                                                                              cACTAATCAGCGTATCCAGCTCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTTCCGTTGTAACCAGCg  <  2:829077/148‑1 (MQ=255)
                                                                                                                                              cACTAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCg  <  2:1751638/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGATTATCGAAAAAGAGTCGGTTATTGAGGCCGGTTTTCACGTCCTGGGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCAGCAATTCTTGCCCGGCAAGTTGCCGTTGTAACCAGCG  >  CP009273/3395937‑3396226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑