Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,423,551 T→A 15.7% Q216L (CAG→CTG)  aroE ← dehydroshikimate reductase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,423,5510TA15.7% 483.5 / 75.2 178Q216L (CAG→CTG) aroEdehydroshikimate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (71/79);  new base A (15/13);  total (86/92)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 178 total aligned reads displayed.

GCTTTATAACTGGTTCTACGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCATACTCAACGCCTGAAT  >  CP009273/3423405‑3423696
                                                                                                                                                  |                                                                                                                                                 
 cTTTATAACTGGTTCTACGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGa                                                                                                                                                 >  1:377811/1‑148 (MQ=255)
   ttATAACTGGTTCTACGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATa                                                                                                                                               >  2:1328153/1‑148 (MQ=255)
     ataACTGGTTCTACGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGa                                                                                                                                             <  2:1765129/148‑1 (MQ=255)
      taACTGGTTCTTCGTCAGTCAGAACACCGTGCCAGAGAAGAAAGGCTTGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCATCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGaa                                                                                                                                            <  1:906485/148‑1 (MQ=255)
       aaCTGGTTCTACGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAAc                                                                                                                                           <  2:1068225/148‑1 (MQ=255)
       aaCTGGTTCTACGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAAc                                                                                                                                           <  2:475070/148‑1 (MQ=255)
       aaCTGGTTCTACGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAAc                                                                                                                                           <  1:3020960/148‑1 (MQ=255)
       aaCTGGTTCTACGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAAc                                                                                                                                           >  2:2487158/1‑148 (MQ=255)
            gTTCTACGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTc                                                                                                                                      >  1:564041/1‑148 (MQ=255)
              tCTACGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCAt                                                                                                                                    >  2:1852333/1‑148 (MQ=255)
                 aCGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGc                                                                                                                                 >  2:8272/1‑148 (MQ=255)
                    tCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCact                                                                                                                              >  2:3296641/1‑146 (MQ=255)
                    tCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAAt                                                                                                                              >  2:3002639/1‑148 (MQ=255)
                    tCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAAt                                                                                                                              >  2:2514091/1‑148 (MQ=255)
                      aGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATaa                                                                                                                            <  2:1886982/148‑1 (MQ=255)
                        gCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAAt                                                                                                                          >  1:2687418/1‑148 (MQ=255)
                        gCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAAt                                                                                                                          >  2:2683238/1‑148 (MQ=255)
                        gCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAAt                                                                                                                          >  1:695779/1‑148 (MQ=255)
                         cAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATg                                                                                                                         <  2:1120063/148‑1 (MQ=255)
                         cAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATg                                                                                                                         <  2:3290717/148‑1 (MQ=255)
                            aaCACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCt                                                                                                                      >  1:128069/1‑148 (MQ=255)
                            aaCACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAATGCCt                                                                                                                      <  1:168841/148‑1 (MQ=255)
                             acacCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTg                                                                                                                     >  2:2371617/1‑148 (MQ=255)
                              cacCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTgg                                                                                                                    <  1:2315088/148‑1 (MQ=255)
                              cacCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTgg                                                                                                                    <  2:1960604/148‑1 (MQ=255)
                               acCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGa                                                                                                                   <  1:42054/148‑1 (MQ=255)
                                    gCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAATGCCTGGatgaat                                                                                                              <  2:2063954/148‑1 (MQ=255)
                                         agaagaAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCg                                                                                                         >  1:1144935/1‑148 (MQ=255)
                                          gaagaaAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGa                                                                                                        <  1:2216738/148‑1 (MQ=255)
                                           aagaaAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCATCGCTGCTCACACCATGCAAGAAAAGGAGTTTTTCATTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGAt                                                                                                       >  1:1952766/1‑148 (MQ=255)
                                            agaaAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATg                                                                                                      <  2:744623/148‑1 (MQ=255)
                                            agaaAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATg                                                                                                      <  2:894970/148‑1 (MQ=255)
                                              aaaGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGAc                                                                                                    >  1:2932490/1‑148 (MQ=255)
                                              aaaGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGAc                                                                                                    >  1:2932507/1‑148 (MQ=255)
                                                  gCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGa                                                                                                >  1:2703163/1‑148 (MQ=255)
                                                  gCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGa                                                                                                >  1:718414/1‑148 (MQ=255)
                                                  gCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGa                                                                                                <  1:3226829/148‑1 (MQ=255)
                                                   cATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGAt                                                                                               <  2:75962/148‑1 (MQ=255)
                                                     tGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCg                                                                                             >  2:707653/1‑148 (MQ=255)
                                                      gAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGc                                                                                            >  2:2798318/1‑148 (MQ=255)
                                                       aGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGcc                                                                                           >  2:2957589/1‑148 (MQ=255)
                                                         ccgccTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCgg                                                                                         >  2:1360970/1‑148 (MQ=255)
                                                         ccgccTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCgg                                                                                         >  1:743486/1‑148 (MQ=255)
                                                         ccgccTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCgg                                                                                         >  1:2271090/1‑148 (MQ=255)
                                                              tgtgCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAatat                                                                                    <  2:2246965/148‑1 (MQ=255)
                                                              tgtgCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAatat                                                                                    <  2:2246912/148‑1 (MQ=255)
                                                               gtgCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATc                                                                                   >  1:2491650/1‑148 (MQ=255)
                                                                   caccaGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATcacca                                                                               <  2:1689503/148‑1 (MQ=255)
                                                                    accaGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATcaccac                                                                              <  2:1086595/148‑1 (MQ=255)
                                                                      caGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTg                                                                            <  1:190864/148‑1 (MQ=255)
                                                                      caGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTg                                                                            >  1:3197855/1‑148 (MQ=255)
                                                                       aGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGa                                                                           >  2:3250009/1‑148 (MQ=255)
                                                                            tCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCa                                                                      >  2:2936742/1‑148 (MQ=255)
                                                                               tAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTg                                                                   >  2:3142225/1‑148 (MQ=255)
                                                                                aaaCCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTgg                                                                  <  1:2873991/148‑1 (MQ=255)
                                                                                 aaCCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGa                                                                 >  1:1409628/1‑148 (MQ=255)
                                                                                 aaCCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGa                                                                 <  1:3218298/148‑1 (MQ=255)
                                                                                    cATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGt                                                              >  2:844076/1‑148 (MQ=255)
                                                                                     aTCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGtt                                                             <  1:745050/148‑1 (MQ=255)
                                                                                      tCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTg                                                            >  1:2773468/1‑148 (MQ=255)
                                                                                      tCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAATGCCTGGATGACTGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTg                                                            >  1:1789109/1‑148 (MQ=255)
                                                                                      tCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTg                                                            >  1:114636/1‑148 (MQ=255)
                                                                                       cAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGc                                                           <  2:2696346/148‑1 (MQ=255)
                                                                                        aGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCa                                                          <  2:1688063/148‑1 (MQ=255)
                                                                                        aGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCa                                                          <  2:58784/148‑1 (MQ=255)
                                                                                        aGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCa                                                          <  2:59075/148‑1 (MQ=255)
                                                                                          cATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCAtt                                                        >  1:2199390/1‑148 (MQ=255)
                                                                                          cATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCAtt                                                        >  1:207732/1‑148 (MQ=255)
                                                                                          cATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCAtt                                                        >  1:1828483/1‑148 (MQ=255)
                                                                                                  tttGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATga                                                <  2:379951/148‑1 (MQ=255)
                                                                                                  tttGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATga                                                >  1:2672607/1‑148 (MQ=255)
                                                                                                   ttGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATgag                                               >  2:2313077/1‑148 (MQ=255)
                                                                                                     gAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGAt                                             <  1:2439379/148‑1 (MQ=255)
                                                                                                       gCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCa                                           >  2:578895/1‑148 (MQ=255)
                                                                                                            gctgctCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTc                                      <  1:707611/148‑1 (MQ=255)
                                                                                                               gctCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATg                                   <  2:1037648/148‑1 (MQ=255)
                                                                                                                  cacaCCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGAcc                                >  2:1530384/1‑148 (MQ=255)
                                                                                                                      ccATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTcc                            <  2:734150/148‑1 (MQ=255)
                                                                                                                       cATGCCAGAAAAGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCa                           >  2:2654081/1‑148 (MQ=255)
                                                                                                                         tGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGt                         <  2:497881/148‑1 (MQ=255)
                                                                                                                          gCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGtt                        <  1:1328067/148‑1 (MQ=255)
                                                                                                                           ccAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTc                       <  2:1230177/148‑1 (MQ=255)
                                                                                                                           ccAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTc                       >  2:1361518/1‑148 (MQ=255)
                                                                                                                             aGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGt                     <  2:2180663/148‑1 (MQ=255)
                                                                                                                             aGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGt                     <  2:574247/148‑1 (MQ=255)
                                                                                                                              gAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTc                    <  2:2558607/148‑1 (MQ=255)
                                                                                                                               aaaaGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTcc                   <  1:1332653/148‑1 (MQ=255)
                                                                                                                               aaaaGGAGTTTTTCCTTTCAGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTcc                   <  2:695779/148‑1 (MQ=255)
                                                                                                                                 aaGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCAt                 >  2:234437/1‑148 (MQ=255)
                                                                                                                                    gAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCATACt              >  2:2866293/1‑148 (MQ=255)
                                                                                                                                       tttttCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCATACTCaa           >  2:26881/1‑148 (MQ=255)
                                                                                                                                          ttCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCATACTCAACGc        <  2:1206935/148‑1 (MQ=255)
                                                                                                                                           tCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCATACTCAACGcc       <  1:1862120/148‑1 (MQ=255)
                                                                                                                                            ccTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCATACTCAACGCCt      >  1:2883840/1‑148 (MQ=255)
                                                                                                                                            ccTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCATACTCAACGCCt      >  1:682036/1‑148 (MQ=255)
                                                                                                                                               ttCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCATACTCAACGCCTGaa   >  2:145869/1‑148 (MQ=255)
                                                                                                                                               ttCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCATACTCAACGCCTGaa   <  1:1852333/148‑1 (MQ=255)
                                                                                                                                               ttCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTACCAGTTCGTCCATACTCAACGCCTGaa   >  2:445672/1‑148 (MQ=255)
                                                                                                                                                tCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATATTGAGATCAAACTCATGACCTTCCAGTTCGTCCATACTCAACGCCTGAAt  <  2:1952766/148‑1 (MQ=255)
                                                                                                                                                tCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCATACTCAACGCCTGAAt  <  1:2806616/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCTTTATAACTGGTTCTACGTCAGGCAGAACACCGTGCCAGAGAAGAAAGGCATGAGCCGCCTGTGCCACCAGCATTCCTAAACCATCAGCATTACGCTTTGAGCCTCGCTGCTCACACCATGCCAGAAAAGGAGTTTTTCCTTTCTGATAGAACATGTCATAGCAATAAATGCCTGGATGAATGAGCGATGACGGGATCGCCGGAATATCACCACTGATGCCACTGGATGTTGCATTAATAATGAGATCAAACTCATGACCTTCCAGTTCGTCCATACTCAACGCCTGAAT  >  CP009273/3423405‑3423696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑