Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,426,905 T→A 5.3% E5D (GAA→GAT)  smf ← DNA recombination‑mediator A family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,426,9050TA 5.3% 639.8 / 19.5 207E5D (GAA→GAT) smfDNA recombination‑mediator A family protein
Reads supporting (aligned to +/- strand):  ref base T (113/83);  new base A (2/9);  total (115/92)
Fisher's exact test for biased strand distribution p-value = 1.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 216 total aligned reads displayed.

TGAAAGAAAGCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGT  >  CP009273/3426758‑3427052
                                                                                                                                                   |                                                                                                                                                   
tGAAAGAAAGCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAAtt                                                                                                                                                     >  2:2887069/1‑148 (MQ=255)
  aaagaaagCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTc                                                                                                                                                   >  2:1588302/1‑148 (MQ=255)
   aagaaagCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCt                                                                                                                                                  <  1:2820502/148‑1 (MQ=255)
   aagaaagCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCt                                                                                                                                                  <  1:983877/148‑1 (MQ=255)
    agaaagCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATATCTg                                                                                                                                                 >  1:438802/1‑148 (MQ=255)
     gaaagCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGt                                                                                                                                                <  2:1346371/148‑1 (MQ=255)
      aaagCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTa                                                                                                                                               >  1:1083786/1‑148 (MQ=255)
        agCGTTGTGCCTGCCGCAATGTAATCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACACAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATc                                                                                                                                             >  2:2105046/1‑148 (MQ=255)
        agCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATc                                                                                                                                             >  1:1431476/1‑148 (MQ=255)
        agCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATc                                                                                                                                             >  2:2476206/1‑148 (MQ=255)
        agCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATc                                                                                                                                             >  1:17343/1‑148 (MQ=255)
          cGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGa                                                                                                                                           <  2:2315935/148‑1 (MQ=255)
            ttGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGAcc                                                                                                                                         >  2:1305213/1‑148 (MQ=255)
             tgtgCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCa                                                                                                                                        >  1:1347550/1‑148 (MQ=255)
                  ctgccGCTATGTAAGCCCTGTTTGCTGCAATACATCCTCATCATTATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTACTGTATCGACCATCCtt                                                                                                                                   <  2:366504/148‑1 (MQ=255)
                  ctgccGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCtt                                                                                                                                   >  2:1160257/1‑148 (MQ=255)
                             tAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGcc                                                                                                                        >  2:2384147/1‑148 (MQ=255)
                             tAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGcc                                                                                                                        >  2:871475/1‑148 (MQ=255)
                                   cTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAagc                                                                                                                  <  2:689137/148‑1 (MQ=255)
                                       ttGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGcc                                                                                                              >  1:1860775/1‑148 (MQ=255)
                                            gcAATACAACCGCATCAATATGCGACTGTGTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGc                                                                                                         >  1:1018681/1‑148 (MQ=255)
                                             cAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCa                                                                                                        <  1:1393480/148‑1 (MQ=255)
                                              aaTACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATATCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCaa                                                                                                       <  2:252625/148‑1 (MQ=255)
                                                tACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATc                                                                                                     >  1:793179/1‑148 (MQ=255)
                                                tACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATc                                                                                                     <  1:28461/148‑1 (MQ=255)
                                                 aCAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACTCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCt                                                                                                    >  1:3049175/1‑148 (MQ=255)
                                                 aCAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCt                                                                                                    >  2:889087/1‑148 (MQ=255)
                                                    aCCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTg                                                                                                 >  2:2757666/1‑148 (MQ=255)
                                                    aCCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTg                                                                                                 >  1:1824084/1‑148 (MQ=255)
                                                      cggaTCAATATGCTAGTGTTTTTCCACGCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTTCTGATACTCCTTAAACGCAGGCAAATTTCTGTATCGACGATCATTATCTGCCTGCCATAAGCAGCCTTAGCAATCTTTGCg                                                                                               <  1:2202491/145‑1 (MQ=255)
                                                         aTCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGAtt                                                                                            <  1:663924/148‑1 (MQ=255)
                                                          tCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTg                                                                                           >  2:632164/1‑148 (MQ=255)
                                                           cAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTgg                                                                                          >  1:121215/1‑148 (MQ=255)
                                                            aaTATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGt                                                                                         <  1:2973021/148‑1 (MQ=255)
                                                            aaTATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATATCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGt                                                                                         <  1:721710/148‑1 (MQ=255)
                                                               atGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAg                                                                                      >  1:763643/1‑148 (MQ=255)
                                                                tGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGt                                                                                     >  2:3178800/1‑148 (MQ=255)
                                                                  cGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGa                                                                                   >  2:197666/1‑148 (MQ=255)
                                                                    aCTGTTTTGCCACCCAGTGAGCGATACGGACCATATCCTCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATg                                                                                 <  1:3189543/148‑1 (MQ=255)
                                                                    aCTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATg                                                                                 <  1:3189440/148‑1 (MQ=255)
                                                                    aCTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATg                                                                                 >  1:1800657/1‑148 (MQ=255)
                                                                     cTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGc                                                                                >  2:1866/1‑148 (MQ=255)
                                                                         tttGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTc                                                                            >  1:1491597/1‑148 (MQ=255)
                                                                                cccAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCaga                                                                     <  2:572487/148‑1 (MQ=255)
                                                                                cccAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCaga                                                                     >  1:2424143/1‑148 (MQ=255)
                                                                                  cAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAgg                                                                   <  2:499203/148‑1 (MQ=255)
                                                                                     tGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAggggg                                                                >  2:1416897/1‑148 (MQ=255)
                                                                                      gAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGa                                                               <  2:855288/148‑1 (MQ=255)
                                                                                      gAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGa                                                               <  2:1083786/148‑1 (MQ=255)
                                                                                      gAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGa                                                               <  2:2831273/148‑1 (MQ=255)
                                                                                        gCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGAtt                                                             <  1:2773536/148‑1 (MQ=255)
                                                                                         cGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGAttt                                                            >  2:1221456/1‑148 (MQ=255)
                                                                                           aTACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGt                                                          <  2:1078778/148‑1 (MQ=255)
                                                                                           aTACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGt                                                          <  1:1921385/148‑1 (MQ=255)
                                                                                           aTACGGACCATATCATCGCCGGACCAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGt                                                          <  2:2675584/148‑1 (MQ=255)
                                                                                             aCGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATATCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCt                                                        <  2:438802/148‑1 (MQ=255)
                                                                                                gACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtaga                                                     <  2:659280/148‑1 (MQ=255)
                                                                                                gACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtaga                                                     <  1:908147/148‑1 (MQ=255)
                                                                                                gACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtaga                                                     <  2:417464/148‑1 (MQ=255)
                                                                                                gACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtaga                                                     <  2:2320165/148‑1 (MQ=255)
                                                                                                 aCCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtagaa                                                    >  1:2043462/1‑148 (MQ=255)
                                                                                                 aCCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtagaa                                                    >  1:2172782/1‑148 (MQ=255)
                                                                                                  ccATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATATCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtagaat                                                   <  1:2816340/148‑1 (MQ=255)
                                                                                                     tatCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtagaataga                                                <  2:2498076/148‑1 (MQ=255)
                                                                                                     tatCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtagaataga                                                <  1:1004831/148‑1 (MQ=255)
                                                                                                     tatCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtagaataga                                                <  1:2658968/148‑1 (MQ=255)
                                                                                                      atcatcGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtagaatagaa                                               >  1:178810/1‑148 (MQ=255)
                                                                                                      atcatcGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCtagaatagaa                                               >  1:2859267/1‑148 (MQ=255)
                                                                                                         atcGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAgaagaa                                            <  1:1912345/148‑1 (MQ=255)
                                                                                                            gCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAaata                                         <  1:1160257/148‑1 (MQ=255)
                                                                                                             ccGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAaataa                                        >  1:2491665/1‑148 (MQ=255)
                                                                                                               gTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATc                                      <  1:1281096/148‑1 (MQ=255)
                                                                                                                tACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCt                                     >  1:2321760/1‑148 (MQ=255)
                                                                                                                  cAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCttt                                   >  1:3209820/1‑148 (MQ=255)
                                                                                                                  cAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCttt                                   >  2:3293043/1‑148 (MQ=255)
                                                                                                                     gctgctGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTa                                <  1:1540985/148‑1 (MQ=255)
                                                                                                                     gctgctGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTa                                >  1:4537/1‑148 (MQ=255)
                                                                                                                     gctgctGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTa                                >  1:1816801/1‑148 (MQ=255)
                                                                                                                      ctgctgATACTCATTAAACGCAGCCAAATTTCTGTATCGACCAGCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTaa                               <  2:2948984/148‑1 (MQ=255)
                                                                                                                        gctgATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACt                             >  1:557097/1‑148 (MQ=255)
                                                                                                                              aCTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGaa                       >  1:1637643/1‑148 (MQ=255)
                                                                                                                                tCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAaca                     >  2:1679087/1‑148 (MQ=255)
                                                                                                                                tCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAaca                     >  1:1961572/1‑148 (MQ=255)
                                                                                                                                 cATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAacac                    >  1:286974/1‑148 (MQ=255)
                                                                                                                                  aTTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAAcaca                   >  1:3273720/1‑148 (MQ=255)
                                                                                                                                   ttAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACAt                  <  2:827061/148‑1 (MQ=255)
                                                                                                                                    tAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACAtc                 <  2:1824084/148‑1 (MQ=255)
                                                                                                                                    tAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACAtc                 >  2:867722/1‑148 (MQ=255)
                                                                                                                                     aaaCGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACAtct                <  2:1026901/148‑1 (MQ=255)
                                                                                                                                      aaCGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACAtctc               <  2:3123011/148‑1 (MQ=255)
                                                                                                                                      aaCGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACAtctc               <  2:268878/148‑1 (MQ=255)
                                                                                                                                       aCGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCCATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATctct              >  2:2244598/1‑148 (MQ=255)
                                                                                                                                       aCGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATctct              >  2:582343/1‑148 (MQ=255)
                                                                                                                                        cGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTg             <  2:1525700/148‑1 (MQ=255)
                                                                                                                                          cAGCCTAATTTCTGTATCGAACATCCTTATCTCACTGACATAAGCAGCCTTAGCAATCTTTGAGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGga           <  1:2105046/148‑1 (MQ=255)
                                                                                                                                            gCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGgaga         <  1:1588302/148‑1 (MQ=255)
                                                                                                                                            gCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGgaga         <  2:3235360/148‑1 (MQ=255)
                                                                                                                                                aaTTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTa     <  1:365576/148‑1 (MQ=255)
                                                                                                                                                 aTTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTAt    >  2:1892163/1‑148 (MQ=255)
                                                                                                                                                   ttCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGt  <  1:2627669/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGAAAGAAAGCGTTGTGCCTGCCGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGT  >  CP009273/3426758‑3427052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑