Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,438,440 T→A 10.6% Y50F (TAC→TTC)  rpsE ← 30S ribosomal subunit protein S5

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,438,4400TA10.6% 626.9 / 50.5 220Y50F (TAC→TTC) rpsE30S ribosomal subunit protein S5
Reads supporting (aligned to +/- strand):  ref base T (110/85);  new base A (11/13);  total (122/98)
Fisher's exact test for biased strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

TGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCA  >  CP009273/3438299‑3438587
                                                                                                                                             |                                                                                                                                                   
  cATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTaaccaaaa                                                                                                                                             >  2:2962671/1‑148 (MQ=255)
   aTGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTaaccaaaac                                                                                                                                            >  2:450089/1‑148 (MQ=255)
    tGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTaaccaaaacc                                                                                                                                           >  1:732232/1‑148 (MQ=255)
    tGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTaaccaaaacc                                                                                                                                           >  1:480219/1‑148 (MQ=255)
      aaTACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTaaccaaaaccaa                                                                                                                                         <  1:3072377/148‑1 (MQ=255)
       aTACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAAc                                                                                                                                        >  2:487811/1‑148 (MQ=255)
        tACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAAcg                                                                                                                                       >  1:2685794/1‑148 (MQ=255)
          cgcgAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAAcgcg                                                                                                                                     <  2:680927/148‑1 (MQ=255)
           gcgAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGa                                                                                                                                    <  2:762713/148‑1 (MQ=255)
            cgAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGAc                                                                                                                                   >  1:1939007/1‑148 (MQ=255)
              agaACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCg                                                                                                                                 >  2:382186/1‑148 (MQ=255)
               gaACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGt                                                                                                                                >  2:596309/1‑148 (MQ=255)
               gaACCCGTGTGAACACATTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGATTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCCACGCGACCGt                                                                                                                                <  1:809161/148‑1 (MQ=255)
                  cccGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTAc                                                                                                                             >  1:2548482/1‑148 (MQ=255)
                   ccGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACTTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTAcc                                                                                                                            <  1:1307455/148‑1 (MQ=255)
                      tgtgAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATc                                                                                                                         >  2:2239068/1‑148 (MQ=255)
                          aaCACCTTTAACAGGGTGTTGCAGAGTGCCGTTATCCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGAAACCAAAACCAACGCGACCGTTACCATCGCCa                                                                                                                     >  2:530929/1‑148 (MQ=255)
                            cacCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAAc                                                                                                                   <  1:1392090/148‑1 (MQ=255)
                             acCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGAAACCAAAACCAACGCGACCGTTACCATCGCCAACt                                                                                                                  >  1:274978/1‑148 (MQ=255)
                             acCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGAAACCAAAACCAACGCGACCGTTACCATCGCCAACt                                                                                                                  >  1:2811139/1‑148 (MQ=255)
                              ccTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGAAACCAAAACCAACGCGACCGTTACCATCGCCAACTa                                                                                                                 >  2:2728143/1‑148 (MQ=255)
                                tttAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACa                                                                                                               <  2:2432989/148‑1 (MQ=255)
                                  tAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGt                                                                                                             >  2:2289795/1‑148 (MQ=255)
                                  tAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGt                                                                                                             <  2:1416915/148‑1 (MQ=255)
                                    aCAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCa                                                                                                           >  1:1625581/1‑148 (MQ=255)
                                    aCAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCa                                                                                                           >  1:1625158/1‑148 (MQ=255)
                                    aCAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCa                                                                                                           >  1:2805887/1‑148 (MQ=255)
                                     cAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCag                                                                                                          <  2:2896014/148‑1 (MQ=255)
                                       gggTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCagag                                                                                                        >  2:426622/1‑148 (MQ=255)
                                       gggTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCagag                                                                                                        >  2:573754/1‑148 (MQ=255)
                                          tgtTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCtg                                                                                                     >  2:1020680/1‑148 (MQ=255)
                                                  agTGCCGTTATTCATCGAGACGTTAATCATAATGCGACGGGCATTTTCCATCGCTTTCTGGAACGCTGCTGGAACTAAACGCGCTTTACCGTAACCAAAACCAACGCGACCTAAACCATCGACAACTAAAGTCAGAGCTGTGAAGgag                                                                                             >  2:822177/1‑148 (MQ=255)
                                                     gCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGaaa                                                                                          <  1:2132543/148‑1 (MQ=255)
                                                       cGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAAt                                                                                        <  2:71322/148‑1 (MQ=255)
                                                        gTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATa                                                                                       <  1:2964110/148‑1 (MQ=255)
                                                        gTTATTCAGCGCGACGTTAATCATATTGCGACGGGATTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATa                                                                                       <  1:153188/148‑1 (MQ=255)
                                                        gTTATTCAGCGCGACGTTAATCATAGTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATa                                                                                       <  1:1471784/148‑1 (MQ=255)
                                                         ttATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATAc                                                                                      >  1:2957442/1‑148 (MQ=255)
                                                           aTTCAGCGTGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAACCCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGa                                                                                    <  2:1895609/148‑1 (MQ=255)
                                                               aGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTCCAGTCAGAGCTGTGAAGGAGAAAATACGaccac                                                                                <  2:1515333/148‑1 (MQ=255)
                                                               aGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGaccac                                                                                >  2:1801626/1‑148 (MQ=255)
                                                                gcgcGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGaccacc                                                                               >  2:1119483/1‑148 (MQ=255)
                                                                gcgcGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGaccacc                                                                               >  1:1007466/1‑148 (MQ=255)
                                                                 cgcgACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCCGAGCTGTGAAGGAGAAAATACGACCACCt                                                                              >  2:2692958/1‑148 (MQ=255)
                                                                 cgcgACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCt                                                                              <  2:3275191/148‑1 (MQ=255)
                                                                   cgACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCttt                                                                            >  2:2005197/1‑148 (MQ=255)
                                                                    taggTTATTCATATTGCGACGGGTTTTTTCCATCGCTTTGTGGATGGCTGCTGTAACTTCACGCGCTTTACCGTAACCAAAACCACCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTa                                                                           <  1:3143252/145‑1 (MQ=255)
                                                                     aCGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTaa                                                                          >  1:1399435/1‑148 (MQ=255)
                                                                      cGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAAc                                                                         >  1:105930/1‑148 (MQ=255)
                                                                        ttAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACgg                                                                       >  1:159981/1‑148 (MQ=255)
                                                                         tAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGt                                                                      <  1:1334095/148‑1 (MQ=255)
                                                                            tCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGtttt                                                                   >  2:2553911/1‑148 (MQ=255)
                                                                            tCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTGTAACGGtttt                                                                   >  2:836603/1‑148 (MQ=255)
                                                                            tCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGAAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGtttt                                                                   <  1:3070547/148‑1 (MQ=255)
                                                                              atatTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAg                                                                 <  1:1220528/148‑1 (MQ=255)
                                                                               tatTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGa                                                                >  1:2851557/1‑148 (MQ=255)
                                                                                atTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGAt                                                               <  2:1037469/148‑1 (MQ=255)
                                                                                atTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGAt                                                               >  1:140402/1‑148 (MQ=255)
                                                                                atTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGAt                                                               <  2:2145062/148‑1 (MQ=255)
                                                                                  tGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATAc                                                             >  1:2881689/1‑148 (MQ=255)
                                                                                    cGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATAcgc                                                           <  2:2402815/148‑1 (MQ=255)
                                                                                     gACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATAcgcg                                                          <  1:2306762/148‑1 (MQ=255)
                                                                                      aCGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCgg                                                         >  1:1041875/1‑148 (MQ=255)
                                                                                          gCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCACCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTa                                                     <  1:461123/148‑1 (MQ=255)
                                                                                          gCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTa                                                     >  1:232228/1‑148 (MQ=255)
                                                                                          gCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTa                                                     >  1:2726623/1‑148 (MQ=255)
                                                                                          gCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTa                                                     >  1:3162464/1‑148 (MQ=255)
                                                                                           cTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTAc                                                    >  1:2414934/1‑148 (MQ=255)
                                                                                             ttttCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGAAACCAAACCCACCGCGACCGTTACCATCGCCAACTCCAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTGTAACGGTTTTAGATACGCGGTTTACcg                                                  <  2:300419/148‑1 (MQ=255)
                                                                                              tttCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACcgc                                                 >  1:841778/1‑148 (MQ=255)
                                                                                                tCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGa                                               <  2:3156276/148‑1 (MQ=255)
                                                                                                 ccatgtttttCTGGTTGGCTGCTGGAACTTCCCGGTCTTTACGTAAACCAAACCCACCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGAt                                              <  1:382186/141‑1 (MQ=255)
                                                                                                 ccATCGCTTTCTGGATCGCGGCTGGAACTACACGCGCTTTACCGAAACAAAAACAAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGAt                                              <  1:2239068/148‑1 (MQ=255)
                                                                                                    tCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAg                                           >  1:873925/1‑148 (MQ=255)
                                                                                                     cGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGc                                          >  1:597497/1‑148 (MQ=255)
                                                                                                       cTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCtt                                        >  2:1268679/1‑148 (MQ=255)
                                                                                                       cTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGAAACCAACACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCtt                                        <  2:2754899/148‑1 (MQ=255)
                                                                                                        tttCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCttt                                       <  1:2476429/148‑1 (MQ=255)
                                                                                                         ttCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTGTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCtttt                                      >  2:3126420/1‑148 (MQ=255)
                                                                                                               aTCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCa                                >  2:1157487/1‑148 (MQ=255)
                                                                                                                tCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCACCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAg                               <  1:3261086/148‑1 (MQ=255)
                                                                                                                tCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAg                               >  1:2333856/1‑148 (MQ=255)
                                                                                                                tCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAg                               <  2:1377806/148‑1 (MQ=255)
                                                                                                                 cGCTGCTGGAACTTCACGCGCTTTACCGAAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGt                              >  2:3267163/1‑148 (MQ=255)
                                                                                                                    tgctgGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCg                           >  1:744096/1‑148 (MQ=255)
                                                                                                                      ctgGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGcc                         >  1:2313461/1‑148 (MQ=255)
                                                                                                                          aaCTTCACGCGCTTTACCGTAACCAAAACCACCGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCt                     <  1:1895015/148‑1 (MQ=255)
                                                                                                                          aaCTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCt                     >  2:2556013/1‑148 (MQ=255)
                                                                                                                          aaCTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTGTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCt                     >  2:2392055/1‑148 (MQ=255)
                                                                                                                            cTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTg                   <  1:40834/148‑1 (MQ=255)
                                                                                                                             ttCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGt                  <  1:2766311/148‑1 (MQ=255)
                                                                                                                             ttCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGt                  <  2:147056/148‑1 (MQ=255)
                                                                                                                               cACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGttt                <  1:2962777/148‑1 (MQ=255)
                                                                                                                                 cgcgCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGttttt              >  2:1044818/1‑148 (MQ=255)
                                                                                                                                 cgcgCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGttttt              <  2:1960848/148‑1 (MQ=255)
                                                                                                                                    gcTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGa           <  2:2938292/148‑1 (MQ=255)
                                                                                                                                    gcTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGa           <  2:1330642/148‑1 (MQ=255)
                                                                                                                                       ttACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGAtgt        >  1:1816449/1‑148 (MQ=255)
                                                                                                                                            gTAACCAAAACCAACGCGTCCGTTACAATCGCCAACTACATTCAGAGCTGTGAAGGAGAAAATACGTCCTCCTTTTACGGTTTTAGATACGCTGTTTACCGCGATCAGCTTTTCATGCAGTTCGCCAGCTTTTTTTTAGATGTGAGca   <  2:1651354/148‑2 (MQ=255)
                                                                                                                                             tAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCa  >  2:883344/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTAACGGTTTTAGATACGCGGTTTACCGCGATCAGCTTTTCCTGCAGTTCGCCAGCTTGTTTTTCGATGTGAGCCA  >  CP009273/3438299‑3438587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑