Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,466,043 C→A 6.2% A277A (GCG→GCT)  fusA ← protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,466,0430CA 6.2% 820.4 / 18.9 219A277A (GCG→GCT) fusAprotein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base C (112/93);  new base A (3/11);  total (115/104)
Fisher's exact test for biased strand distribution p-value = 2.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

GTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCAGAAGCT  >  CP009273/3465897‑3466190
                                                                                                                                                  |                                                                                                                                                   
gTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCg                                                                                                                                                    >  1:2501334/1‑148 (MQ=255)
  aGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGcc                                                                                                                                                  <  2:3207027/148‑1 (MQ=255)
  aGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGATCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGcc                                                                                                                                                  >  2:615865/1‑148 (MQ=255)
     gATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGa                                                                                                                                               <  2:264266/148‑1 (MQ=255)
        tttGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAaca                                                                                                                                            >  2:2099752/1‑148 (MQ=255)
          tGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACAcc                                                                                                                                          <  1:1923872/148‑1 (MQ=255)
           gAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCt                                                                                                                                         <  2:2882588/148‑1 (MQ=255)
           gAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCt                                                                                                                                         <  2:2882619/148‑1 (MQ=255)
            aaCGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCtt                                                                                                                                        >  1:1834982/1‑148 (MQ=255)
            aaCGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATAGCCTGAACACCtt                                                                                                                                        <  2:846702/148‑1 (MQ=255)
                 cAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTc                                                                                                                                   >  2:2113112/1‑148 (MQ=255)
                 cAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTc                                                                                                                                   >  2:2113205/1‑148 (MQ=255)
                 cAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATAGCCTGAACACCTTTGTTc                                                                                                                                   <  2:844678/148‑1 (MQ=255)
                    tGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTg                                                                                                                                >  2:2659021/1‑148 (MQ=255)
                    tGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTg                                                                                                                                >  1:2285462/1‑148 (MQ=255)
                     gCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGa                                                                                                                               <  1:595508/148‑1 (MQ=255)
                      cAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGaa                                                                                                                              <  2:527057/148‑1 (MQ=255)
                        gagaACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACg                                                                                                                            <  2:3183158/148‑1 (MQ=255)
                         agaACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGc                                                                                                                           >  2:1443521/1‑148 (MQ=255)
                          gaACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCa                                                                                                                          >  1:1086135/1‑148 (MQ=255)
                            aCGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGa                                                                                                                        <  2:665859/148‑1 (MQ=255)
                                cTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAAccc                                                                                                                    >  2:585618/1‑147 (MQ=255)
                                   gTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAg                                                                                                                 <  1:1887614/148‑1 (MQ=255)
                                   gTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAg                                                                                                                 <  1:524942/148‑1 (MQ=255)
                                    tcatcaCTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAgg                                                                                                                >  2:1111706/1‑148 (MQ=255)
                                      atcaCTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGtt                                                                                                              >  2:1035823/1‑148 (MQ=255)
                                        ctcTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTAc                                                                                                            >  1:2099251/3‑148 (MQ=255)
                                        caCTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTAc                                                                                                            <  2:2443671/148‑1 (MQ=255)
                                        caCTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTAc                                                                                                            >  1:1319114/1‑148 (MQ=255)
                                         aCTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTAcc                                                                                                           >  2:641803/1‑148 (MQ=255)
                                          cTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCa                                                                                                          <  1:1534727/148‑1 (MQ=255)
                                           ttGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAg                                                                                                         >  1:2745056/1‑148 (MQ=255)
                                               gTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGgatg                                                                                                     <  1:376459/148‑1 (MQ=255)
                                               gTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGgatg                                                                                                     <  2:1650471/148‑1 (MQ=255)
                                                tGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGgatga                                                                                                    <  1:2397458/148‑1 (MQ=255)
                                                 gACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGAGACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGgatgat                                                                                                   <  2:2099111/148‑1 (MQ=255)
                                                     ttCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCg                                                                                               >  1:304627/1‑148 (MQ=255)
                                                        aGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCgttg                                                                                            <  1:1592336/148‑1 (MQ=255)
                                                          ccGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCgttgtt                                                                                          <  1:1565347/148‑1 (MQ=255)
                                                          ccGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCgttgtt                                                                                          >  2:1739233/1‑148 (MQ=255)
                                                          ccGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCgttgtt                                                                                          >  1:3022273/1‑148 (MQ=255)
                                                           cGGAGTGTCTGTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTc                                                                                         <  2:601228/148‑1 (MQ=255)
                                                             gAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAg                                                                                       >  2:195347/1‑148 (MQ=255)
                                                              aGTGTCTTTACCGTCGTCCAGGATACAGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGa                                                                                      >  2:1677790/1‑148 (MQ=255)
                                                              aGTGTCTTTACCGTCGTCCAGGATACAGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGa                                                                                      <  2:1551663/148‑1 (MQ=255)
                                                                 gtCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAAcg                                                                                   >  2:1904761/1‑148 (MQ=255)
                                                                 gtCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAAcg                                                                                   <  1:1111932/148‑1 (MQ=255)
                                                                 gtCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAAcg                                                                                   <  2:137566/148‑1 (MQ=255)
                                                                    tttACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACgcgc                                                                                <  2:2962744/148‑1 (MQ=255)
                                                                       aCCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGa                                                                             <  1:2923496/148‑1 (MQ=255)
                                                                       aCCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGa                                                                             <  1:1274189/148‑1 (MQ=255)
                                                                           tcgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCa                                                                         <  1:884158/148‑1 (MQ=255)
                                                                            cgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCag                                                                        >  1:2583513/1‑148 (MQ=255)
                                                                            cgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCag                                                                        <  2:2998016/148‑1 (MQ=255)
                                                                            cgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCag                                                                        >  2:641066/1‑148 (MQ=255)
                                                                              tcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCagag                                                                      <  2:2285462/148‑1 (MQ=255)
                                                                                 aGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCAc                                                                   >  2:3282566/1‑148 (MQ=255)
                                                                                 aGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCAc                                                                   >  1:3220577/1‑148 (MQ=255)
                                                                                  ggATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCAcc                                                                  >  2:40567/1‑148 (MQ=255)
                                                                                  ggATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCAcc                                                                  >  2:38889/1‑148 (MQ=255)
                                                                                  ggATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCAcc                                                                  <  2:1626395/148‑1 (MQ=255)
                                                                                   gATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCt                                                                 >  2:2539061/1‑148 (MQ=255)
                                                                                   gATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCt                                                                 >  2:2327624/1‑148 (MQ=255)
                                                                                    aTACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCtt                                                                >  1:436554/1‑148 (MQ=255)
                                                                                     tACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCttt                                                               >  1:2912840/1‑148 (MQ=255)
                                                                                      aCCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTg                                                              >  1:3288672/1‑148 (MQ=255)
                                                                                      aCCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTg                                                              >  1:3067908/1‑148 (MQ=255)
                                                                                      aCCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTg                                                              >  1:2975774/1‑148 (MQ=255)
                                                                                       ccGTTGATCGCAGGTACGTCAACGGGGGATGGCAGGTAATCAATTACCCCATCCAGCATCGCCTGAACACCATTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCATTGa                                                             <  2:1160695/148‑1 (MQ=255)
                                                                                          ttGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGAttt                                                          <  2:1775776/148‑1 (MQ=255)
                                                                                          ttGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGAttt                                                          <  2:2561813/148‑1 (MQ=255)
                                                                                          ttGATCGCAGGTACGTCAACCGGGGATGGCAGGTAAACAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCATAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTGTGAttt                                                          >  1:2475411/1‑148 (MQ=255)
                                                                                             aTCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTg                                                       <  2:2252018/148‑1 (MQ=255)
                                                                                              tCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGc                                                      >  1:585905/1‑148 (MQ=255)
                                                                                              tCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGc                                                      >  1:2248323/1‑148 (MQ=255)
                                                                                                          cAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGttc                                          >  1:1657414/1‑148 (MQ=255)
                                                                                                            aCCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGttctt                                        >  2:1224702/1‑148 (MQ=255)
                                                                                                            aCCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGttctt                                        >  1:907038/1‑148 (MQ=255)
                                                                                                             ccGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGttcttc                                       <  2:477388/148‑1 (MQ=255)
                                                                                                             ccGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGttcttc                                       >  1:929415/1‑148 (MQ=255)
                                                                                                             ccGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGttcttc                                       >  1:1234770/1‑148 (MQ=255)
                                                                                                                   aTGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCaccacc                                 >  2:342050/1‑148 (MQ=255)
                                                                                                                     ggCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCa                               >  1:2535562/1‑148 (MQ=255)
                                                                                                                     ggCAGGTAATCAATTACCGCATCCAGCATAGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCa                               <  1:1805349/148‑1 (MQ=255)
                                                                                                                       cAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAgg                             >  2:884042/1‑148 (MQ=255)
                                                                                                                       cAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAgg                             >  2:3127058/1‑148 (MQ=255)
                                                                                                                         ggTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTa                           >  2:607919/1‑148 (MQ=255)
                                                                                                                            aaTCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTAttt                        <  1:615865/148‑1 (MQ=255)
                                                                                                                             aTCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTAtttt                       <  2:3253003/148‑1 (MQ=255)
                                                                                                                             aTCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTAtttt                       >  2:2425254/1‑148 (MQ=255)
                                                                                                                               cAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTc                     >  2:2637394/1‑148 (MQ=255)
                                                                                                                               cAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTc                     >  2:1789719/1‑148 (MQ=255)
                                                                                                                                   tACCGCATCCAGCATAGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTAACCACCCAGTTATATTTACATc                 >  1:1961036/1‑148 (MQ=255)
                                                                                                                                       gCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGct             <  1:65949/148‑1 (MQ=255)
                                                                                                                                        cATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGctc            >  1:1753238/1‑148 (MQ=255)
                                                                                                                                         aTCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGctct           >  1:366721/1‑148 (MQ=255)
                                                                                                                                           ccAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTc         <  1:3173385/148‑1 (MQ=255)
                                                                                                                                               cATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCAGaa     >  2:607635/1‑148 (MQ=255)
                                                                                                                                                 tCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCAGAAGc   <  2:1145627/148‑1 (MQ=255)
                                                                                                                                                 tCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCAGAAGc   <  2:1293038/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGATTTCTGCTTCAGTCAGTTCTTCACCACCCAGGTATTTTTCCATCAGCTCTTCAGAAGCT  >  CP009273/3465897‑3466190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑