Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,479,769 A→T 100% E97D (GAA→GAT)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,479,7690AT100.0% 650.5 / NA 194E97D (GAA→GAT) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (98/96);  total (98/96)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 194 total aligned reads displayed.

AGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCA  >  CP009273/3479625‑3479913
                                                                                                                                                |                                                                                                                                                
    cTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGt                                                                                                                                           <  1:2918001/148‑1 (MQ=255)
       aTCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACa                                                                                                                                        >  2:1037516/1‑148 (MQ=255)
        tCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCTAACTGGGCCTTTTTGAAGAGGTCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTAGTACaa                                                                                                                                       >  2:722473/1‑148 (MQ=255)
          aaaGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaaa                                                                                                                                     <  1:1035143/148‑1 (MQ=255)
           aaGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaaaa                                                                                                                                    <  2:387005/148‑1 (MQ=255)
           aaGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACaaaaa                                                                                                                                    <  2:816405/148‑1 (MQ=255)
              tcGAAGAGGGTAAAGAAATGATCCTCTCCTATCTTAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAAtt                                                                                                                                 <  2:2523343/147‑1 (MQ=255)
               cGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAAttt                                                                                                                                >  1:738564/1‑148 (MQ=255)
                  agagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGc                                                                                                                             >  2:1337046/1‑148 (MQ=255)
                    agGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTGTTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCa                                                                                                                           >  2:1601644/1‑148 (MQ=255)
                    agGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCa                                                                                                                           <  2:1298231/148‑1 (MQ=255)
                    agGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCa                                                                                                                           >  2:1601637/1‑148 (MQ=255)
                      ggTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAAt                                                                                                                         <  1:1174857/148‑1 (MQ=255)
                        tAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTg                                                                                                                       <  1:1322243/148‑1 (MQ=255)
                        tAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTg                                                                                                                       >  1:416147/1‑148 (MQ=255)
                         aaaGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGa                                                                                                                      >  2:257473/1‑148 (MQ=255)
                         aaaGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGa                                                                                                                      >  2:798769/1‑148 (MQ=255)
                           aGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGAtt                                                                                                                    <  2:897949/148‑1 (MQ=255)
                           aGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGAtt                                                                                                                    <  2:1346281/148‑1 (MQ=255)
                             aaaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCa                                                                                                                  >  1:344659/1‑148 (MQ=255)
                             aaaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCa                                                                                                                  >  2:525189/1‑148 (MQ=255)
                             aaaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCa                                                                                                                  >  1:2186545/1‑148 (MQ=255)
                              aaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAg                                                                                                                 >  1:2202807/1‑148 (MQ=255)
                               aTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGTCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAgg                                                                                                                <  1:2999522/148‑1 (MQ=255)
                                tGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGt                                                                                                               >  2:2479511/1‑148 (MQ=255)
                                tGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGt                                                                                                               <  2:3108092/148‑1 (MQ=255)
                                tGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGt                                                                                                               >  1:2842464/1‑148 (MQ=255)
                                tGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGt                                                                                                               <  2:507380/148‑1 (MQ=255)
                                  aTCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTaa                                                                                                             >  2:1095741/1‑148 (MQ=255)
                                  aTCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTaa                                                                                                             >  2:3111877/1‑148 (MQ=255)
                                   tCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTaaa                                                                                                            <  1:1851832/148‑1 (MQ=255)
                                    ccTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAc                                                                                                           >  2:887082/1‑148 (MQ=255)
                                       ctcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCg                                                                                                        >  1:1480600/1‑148 (MQ=255)
                                         ccTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGa                                                                                                      <  2:108501/148‑1 (MQ=255)
                                         ccTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGa                                                                                                      >  1:741633/1‑148 (MQ=255)
                                          cTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                                                                                                     <  1:206088/148‑1 (MQ=255)
                                           tATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                                                                    <  1:105340/148‑1 (MQ=255)
                                           tATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACa                                                                                                    <  1:1740366/148‑1 (MQ=255)
                                            aTCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                                                                   <  1:1564321/148‑1 (MQ=255)
                                            aTCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                                                                   >  2:648927/1‑148 (MQ=255)
                                             tCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                                                                  <  1:3251020/148‑1 (MQ=255)
                                              cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                 <  2:2291895/148‑1 (MQ=255)
                                              cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                 <  2:2456410/148‑1 (MQ=255)
                                              cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                 <  2:1306505/148‑1 (MQ=255)
                                              cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                 >  2:1234300/1‑148 (MQ=255)
                                              cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc                                                                                                 <  2:2998388/148‑1 (MQ=255)
                                                gAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTg                                                                                               <  2:2967464/148‑1 (MQ=255)
                                                 aaTCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGa                                                                                              <  1:2228371/148‑1 (MQ=255)
                                                  aTCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAt                                                                                             >  1:374647/1‑148 (MQ=255)
                                                   tCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATg                                                                                            >  2:1911039/1‑148 (MQ=255)
                                                   tCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATg                                                                                            >  2:2560973/1‑148 (MQ=255)
                                                    cAGGGTGATTTTATTGGCGAACAGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGc                                                                                           >  2:208202/1‑148 (MQ=255)
                                                       ggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                                                                        <  2:3299917/148‑1 (MQ=255)
                                                       ggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt                                                                                        >  2:1268188/1‑148 (MQ=255)
                                                          gATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGt                                                                                     >  2:2144599/1‑148 (MQ=255)
                                                          gATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGt                                                                                     <  2:1302035/148‑1 (MQ=255)
                                                            ttttATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCt                                                                                   >  1:2042755/1‑148 (MQ=255)
                                                            ttttATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCt                                                                                   >  1:2457156/1‑148 (MQ=255)
                                                               tATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGca                                                                                <  1:2429606/148‑1 (MQ=255)
                                                                    gCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                                                                           >  1:1619196/1‑148 (MQ=255)
                                                                    gCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                                                                           >  1:982955/1‑148 (MQ=255)
                                                                    gCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGAt                                                                           >  1:981240/1‑148 (MQ=255)
                                                                        aCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcg                                                                       <  2:1480600/148‑1 (MQ=255)
                                                                           gggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                                                                    <  1:3055767/148‑1 (MQ=255)
                                                                           gggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                                                                    >  1:3284091/1‑148 (MQ=255)
                                                                            ggCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtc                                                                   >  1:1351387/1‑148 (MQ=255)
                                                                              ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtcgt                                                                 <  1:2542394/148‑1 (MQ=255)
                                                                              ccTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtcgt                                                                 >  2:3066695/1‑148 (MQ=255)
                                                                               cTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCgtcgtc                                                                <  2:862760/148‑1 (MQ=255)
                                                                                  tttGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc                                                             >  1:1151563/1‑148 (MQ=255)
                                                                                        gagGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCa                                                       >  1:3122618/1‑148 (MQ=255)
                                                                                        gagGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCa                                                       >  2:1307843/1‑148 (MQ=255)
                                                                                         agGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCAc                                                      >  1:2605172/1‑148 (MQ=255)
                                                                                           ggCCAGGAACGTAGCGCATGGGTACGTTCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACCGATGGCGCGTCGTCTGCAAGTCACtt                                                    >  1:2162632/1‑148 (MQ=255)
                                                                                               aGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCaga                                                >  1:165206/1‑148 (MQ=255)
                                                                                                  aaCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGaa                                             >  1:207797/1‑148 (MQ=255)
                                                                                                    cGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAg                                           >  2:1643179/1‑148 (MQ=255)
                                                                                                     gTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGt                                          <  1:1955099/148‑1 (MQ=255)
                                                                                                      tAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTg                                         <  2:2377789/148‑1 (MQ=255)
                                                                                                        gcgcATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTggg                                       <  1:3013757/148‑1 (MQ=255)
                                                                                                              gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCt                                 <  2:2115397/148‑1 (MQ=255)
                                                                                                              gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCt                                 <  1:1272410/148‑1 (MQ=255)
                                                                                                              gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCt                                 <  1:605876/148‑1 (MQ=255)
                                                                                                              gggTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCt                                 <  1:1601644/148‑1 (MQ=255)
                                                                                                                gTACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTgg                               <  2:702173/148‑1 (MQ=255)
                                                                                                                 tACGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGc                              >  1:1395258/1‑148 (MQ=255)
                                                                                                                  aCGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCg                             <  1:2793055/148‑1 (MQ=255)
                                                                                                                   cGTGCGAAAACCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGt                            <  1:887082/148‑1 (MQ=255)
                                                                                                                         aaaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCg                      >  1:808143/1‑148 (MQ=255)
                                                                                                                         aaaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCg                      <  2:612436/148‑1 (MQ=255)
                                                                                                                          aaaCCGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGa                     <  2:1166980/148‑1 (MQ=255)
                                                                                                                             ccGCCTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGt                  >  1:2685393/1‑148 (MQ=255)
                                                                                                                                ccTGTGAAGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGAc               >  2:1409029/1‑148 (MQ=255)
                                                                                                                                       aGTGGCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGc        <  1:3056996/148‑1 (MQ=255)
                                                                                                                                           gCTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTg    >  2:2263394/1‑148 (MQ=255)
                                                                                                                                            cTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGc   <  1:2956284/148‑1 (MQ=255)
                                                                                                                                            cTGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGc   >  1:668259/1‑148 (MQ=255)
                                                                                                                                             tGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGca  <  1:2164401/148‑1 (MQ=255)
                                                                                                                                             tGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGca  >  2:3037881/1‑148 (MQ=255)
                                                                                                                                             tGATATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGca  <  2:1039879/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCA  >  CP009273/3479625‑3479913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑