Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,510,311 C→A 16.1% G119C (GGT→TGT)  damX ← cell division protein that binds to the septal ring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,510,3110CA16.1% 519.3 / 58.5 174G119C (GGT→TGT) damXcell division protein that binds to the septal ring
Reads supporting (aligned to +/- strand):  ref base C (69/77);  new base A (10/18);  total (79/95)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 198 total aligned reads displayed.

GAGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTC  >  CP009273/3510164‑3510458
                                                                                                                                                   |                                                                                                                                                   
gAGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAaccgatacc                                                                                                                                                     >  2:2555466/1‑148 (MQ=255)
 aGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCTCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAaccgataccg                                                                                                                                                    <  2:1969124/148‑1 (MQ=255)
 aGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAaccgataccg                                                                                                                                                    >  2:1030546/1‑148 (MQ=255)
 aGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAaccgataccg                                                                                                                                                    <  1:2901337/148‑1 (MQ=255)
 aGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAaccgataccg                                                                                                                                                    <  2:2730803/148‑1 (MQ=255)
   tATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAaccgataccgat                                                                                                                                                  <  1:788698/148‑1 (MQ=255)
   tATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAaccgataccgat                                                                                                                                                  >  1:2895552/1‑148 (MQ=255)
    aTTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCgatg                                                                                                                                                 <  1:3226177/148‑1 (MQ=255)
    aTTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCgatg                                                                                                                                                 <  1:2793302/148‑1 (MQ=255)
     ttttCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCgatga                                                                                                                                                >  2:3034059/1‑148 (MQ=255)
                cGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGt                                                                                                                                     <  2:2706708/148‑1 (MQ=255)
                 gTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTa                                                                                                                                    <  2:1702062/148‑1 (MQ=255)
                      tCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAAcc                                                                                                                               <  1:768126/148‑1 (MQ=255)
                      tCCCGGCGCTGGCTGCACGACATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGATTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAAcc                                                                                                                               >  2:1075293/1‑148 (MQ=255)
                        ccGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAg                                                                                                                             >  2:2300675/1‑148 (MQ=255)
                         cGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGa                                                                                                                            <  1:807929/148‑1 (MQ=255)
                         cGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGa                                                                                                                            <  2:1024987/148‑1 (MQ=255)
                          ggCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGaa                                                                                                                           >  1:911841/1‑148 (MQ=255)
                            cgcTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATg                                                                                                                         >  1:2030604/1‑148 (MQ=255)
                                 gctgCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGAc                                                                                                                    >  2:763038/1‑148 (MQ=255)
                                 gctgCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGAc                                                                                                                    >  1:1304450/1‑148 (MQ=255)
                                     cACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGccc                                                                                                                <  2:704765/148‑1 (MQ=255)
                                        gCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCcatc                                                                                                             <  1:2734/148‑1 (MQ=255)
                                        gCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCcatc                                                                                                             <  1:2951206/148‑1 (MQ=255)
                                            ttCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCcatcatca                                                                                                         <  2:1131290/148‑1 (MQ=255)
                                            ttCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCcatcatca                                                                                                         >  1:593543/1‑148 (MQ=255)
                                             tCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCcatcatcat                                                                                                        <  2:234605/148‑1 (MQ=255)
                                              cGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCACAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCata                                                                                                       >  1:1403768/1‑148 (MQ=255)
                                                ccTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCAtata                                                                                                     <  2:2630326/148‑1 (MQ=255)
                                                ccTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCAtata                                                                                                     <  1:34035/148‑1 (MQ=255)
                                                ccTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCAtata                                                                                                     <  1:123898/148‑1 (MQ=255)
                                                  tGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACt                                                                                                   >  1:2688993/1‑148 (MQ=255)
                                                  tGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACt                                                                                                   >  1:2446118/1‑148 (MQ=255)
                                                    aTCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGa                                                                                                 >  1:803087/1‑148 (MQ=255)
                                                    aTCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCACAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGa                                                                                                 <  2:1404160/148‑1 (MQ=255)
                                                      cGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACg                                                                                               <  2:1066463/148‑1 (MQ=255)
                                                       ggTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACga                                                                                              <  1:1543105/148‑1 (MQ=255)
                                                         tCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACgaga                                                                                            <  2:69304/148‑1 (MQ=255)
                                                         tCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACgaga                                                                                            >  2:2395247/1‑148 (MQ=255)
                                                           gCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAg                                                                                          <  1:676269/148‑1 (MQ=255)
                                                           gCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAg                                                                                          <  1:2066787/148‑1 (MQ=255)
                                                              ttGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGTGGGATTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCgg                                                                                       >  1:1640929/1‑148 (MQ=255)
                                                              ttGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCgg                                                                                       >  1:2383734/1‑148 (MQ=255)
                                                              ttGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCgg                                                                                       <  2:3020032/148‑1 (MQ=255)
                                                               tGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCggg                                                                                      >  2:1301093/1‑148 (MQ=255)
                                                               tGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCggg                                                                                      <  1:1045888/148‑1 (MQ=255)
                                                               tGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCggg                                                                                      >  1:1659624/1‑148 (MQ=255)
                                                                gCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGt                                                                                     <  1:1332739/148‑1 (MQ=255)
                                                                   aGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTa                                                                                  >  1:2209939/1‑148 (MQ=255)
                                                                    gCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTAc                                                                                 >  1:1599963/1‑148 (MQ=255)
                                                                        gATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGgc                                                                             >  2:1562980/1‑148 (MQ=255)
                                                                        gATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGgc                                                                             >  1:2074385/1‑148 (MQ=255)
                                                                        gATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGgc                                                                             >  1:235090/1‑148 (MQ=255)
                                                                          tCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGgctg                                                                           <  2:2135240/148‑1 (MQ=255)
                                                                          tCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGgctg                                                                           >  2:1371918/1‑148 (MQ=255)
                                                                           cAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGgctgc                                                                          <  2:3234894/148‑1 (MQ=255)
                                                                            aaTACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGgctgct                                                                         <  2:1070199/148‑1 (MQ=255)
                                                                              tACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCttt                                                                       >  2:464428/1‑148 (MQ=255)
                                                                               aCTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCtttt                                                                      <  1:2615578/148‑1 (MQ=255)
                                                                                ctctTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCttttt                                                                     >  2:1480111/1‑148 (MQ=255)
                                                                                  cttctCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTg                                                                   >  1:984590/1‑148 (MQ=255)
                                                                                  cttctCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTg                                                                   >  2:2517589/1‑148 (MQ=255)
                                                                                  cttctCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTg                                                                   >  1:257013/1‑148 (MQ=255)
                                                                                    tctcGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACAGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTgcg                                                                 >  2:2266308/1‑148 (MQ=255)
                                                                                     ctcGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTgcgc                                                                <  1:1382137/148‑1 (MQ=255)
                                                                                        gCCAGACGCGGATTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTa                                                             <  2:2094908/148‑1 (MQ=255)
                                                                                            gACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGATGTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATAATATACTGACGAGAAGCGGTTTTACTGGCTGCTTTTTTGCGCTTActcg                                                         >  1:1930780/1‑148 (MQ=255)
                                                                                             aCGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCgg                                                        >  1:1019930/1‑148 (MQ=255)
                                                                                             aCGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCgg                                                        <  2:793492/148‑1 (MQ=255)
                                                                                             aCGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCgg                                                        >  1:1844079/1‑148 (MQ=255)
                                                                                             aCGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCCGCTTTTTTGCGCTTACGCgg                                                        <  1:1024162/148‑1 (MQ=255)
                                                                                               gcgGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGgac                                                      >  2:2871736/1‑148 (MQ=255)
                                                                                                   tttGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGacgacg                                                  <  1:2555466/148‑1 (MQ=255)
                                                                                                   tttGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGacgacg                                                  >  1:3111627/1‑148 (MQ=255)
                                                                                                   tttGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACGGGCTGCTTTTTTGCGCTTACGCGGacgacg                                                  <  2:2540054/148‑1 (MQ=255)
                                                                                                    ttGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGc                                                 <  2:1805088/148‑1 (MQ=255)
                                                                                                    ttGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGc                                                 <  2:132359/148‑1 (MQ=255)
                                                                                                     tGATCGGTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGct                                                <  2:3279504/148‑1 (MQ=255)
                                                                                                          gCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTAcg                                           <  1:105538/148‑1 (MQ=255)
                                                                                                            tGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTAcgcg                                         <  2:2209939/148‑1 (MQ=255)
                                                                                                             ggAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACgcgc                                        >  1:3013879/1‑148 (MQ=255)
                                                                                                                aGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGctct                                     >  2:1670832/1‑148 (MQ=255)
                                                                                                                  tGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTc                                   >  2:2879921/1‑148 (MQ=255)
                                                                                                                      cGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATcc                               <  2:653126/148‑1 (MQ=255)
                                                                                                                         gggggCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACg                            >  1:1205349/1‑148 (MQ=255)
                                                                                                                         gggggCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACg                            >  2:65912/1‑148 (MQ=255)
                                                                                                                         gggggCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACg                            >  2:1516083/1‑148 (MQ=255)
                                                                                                                             gCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGttt                        <  1:684915/148‑1 (MQ=255)
                                                                                                                              cTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTc                       >  1:106417/1‑148 (MQ=255)
                                                                                                                                tttAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGctc                                      >  1:2365943/1‑131 (MQ=255)
                                                                                                                                tttAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCAt                     <  2:679772/148‑1 (MQ=255)
                                                                                                                                       cAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGa              >  2:412110/1‑148 (MQ=255)
                                                                                                                                         gAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGAtt            >  2:1617385/1‑148 (MQ=255)
                                                                                                                                            ccgataccgatGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGt         <  1:1433753/148‑1 (MQ=255)
                                                                                                                                            ccgataccgatGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGt         >  1:1287224/1‑148 (MQ=255)
                                                                                                                                            ccgataccgatGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGt         <  1:445409/148‑1 (MQ=255)
                                                                                                                                              gataccgatGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCa       <  2:2364268/148‑1 (MQ=255)
                                                                                                                                              gataccgatGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCa       <  1:763038/148‑1 (MQ=255)
                                                                                                                                                taccgatGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAAt     >  1:66159/1‑148 (MQ=255)
                                                                                                                                                taccgatGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAAt     <  1:944551/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAGTATTTTCCGCAGACGTGGTTCCCGGCGCTGGCTGCACGCCATTCGCCTGATCGGTCGCATTGCCAGCAAGATCAATACTCTTCTCGCCAGACGCGGTTTGATCGCTGGAAGTGGTCGAGGGGGCTTTTAGCGCAGAACCGATACCGATGATCAACAGCAGTAGAACCAGAATGCCGACGCCCATCATCATATACTGACGAGAAGCGGGTTTACTGGCTGCTTTTTTGCGCTTACGCGGACGACGCTCTACGCGCTCTTCATCCACGGTTTCATCTTCGGATTCGTCAATTTC  >  CP009273/3510164‑3510458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑