Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,527,619 G→T 6.2% A481A (GCG→GCT)  pck → phosphoenolpyruvate carboxykinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,527,6190GT 6.2% 756.1 / 16.4 202A481A (GCG→GCT) pckphosphoenolpyruvate carboxykinase
Reads supporting (aligned to +/- strand):  ref base G (113/76);  new base T (9/4);  total (122/80)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

GGCGCGCAGGCTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGCGG  >  CP009273/3527473‑3527764
                                                                                                                                                  |                                                                                                                                                 
ggcgCGCAGGCTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGa                                                                                                                                                  >  1:1018329/1‑148 (MQ=255)
ggcgCGCAGGCTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGa                                                                                                                                                  <  1:3219487/148‑1 (MQ=255)
ggcgCGCAGGCTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGa                                                                                                                                                  >  1:2941057/1‑148 (MQ=255)
ggcgCGCAGGCTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGa                                                                                                                                                  <  1:2760193/148‑1 (MQ=255)
  cgcgcAGGCTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATc                                                                                                                                                <  1:1981403/148‑1 (MQ=255)
  cgcgcAGGCTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATc                                                                                                                                                <  1:1981325/148‑1 (MQ=255)
   gcgcAGGCTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATcc                                                                                                                                               <  1:2122736/148‑1 (MQ=255)
      cAGGCTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCTATCCCaa                                                                                                                                            <  2:738718/148‑1 (MQ=255)
         gCTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCg                                                                                                                                         <  1:2885001/148‑1 (MQ=255)
          cTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGa                                                                                                                                        >  1:1082105/1‑148 (MQ=255)
           ttATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGaa                                                                                                                                       <  1:1191583/148‑1 (MQ=255)
              tCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTg                                                                                                                                    <  1:880866/148‑1 (MQ=255)
              tCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTg                                                                                                                                    >  2:3260304/1‑148 (MQ=255)
               cTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGc                                                                                                                                   <  2:1735165/148‑1 (MQ=255)
                 ggTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGACCTGCCg                                                                                                                                 >  1:2245609/1‑148 (MQ=255)
                  gTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCgg                                                                                                                                <  2:1325613/148‑1 (MQ=255)
                      acacTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGt                                                                                                                            <  1:788009/148‑1 (MQ=255)
                      acacTGGCTGGAACGGCACTGGAAAACTTATCTCTATTAAAGATACCCGCGACTTTATCGACGCCATCCTCAACGGTTCGCTGTATATTGCAGAAACCTTCACTCTGCCGATTTTTAACCTGGCGATCCCAACCGAACTGCCGGGctt                                                                                                                            <  2:3169672/148‑3 (MQ=255)
                          tggctggAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGac                                                                                                                        >  1:128693/1‑148 (MQ=255)
                           ggctggAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGaca                                                                                                                       <  2:2188564/148‑1 (MQ=255)
                           ggctggAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGaca                                                                                                                       <  2:2670041/148‑1 (MQ=255)
                              tggAACGGCACTGGCAAACGTATCTCTATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGa                                                                                                                    <  1:2503470/148‑1 (MQ=255)
                                gAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAg                                                                                                                  <  2:21053/148‑1 (MQ=255)
                                gAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAg                                                                                                                  <  2:1303768/148‑1 (MQ=255)
                                 aaCGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGa                                                                                                                 >  1:1732335/1‑148 (MQ=255)
                                        cTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGa                                                                                                          >  2:3152720/1‑148 (MQ=255)
                                        cTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGa                                                                                                          <  1:2078865/148‑1 (MQ=255)
                                        cTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGa                                                                                                          >  1:11450/1‑148 (MQ=255)
                                        cTGGCAAACGTATCTCGATGAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGa                                                                                                          <  2:1879361/148‑1 (MQ=255)
                                         tGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGAt                                                                                                         <  2:104799/148‑1 (MQ=255)
                                                cGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTa                                                                                                  <  2:2768641/148‑1 (MQ=255)
                                                cGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTa                                                                                                  >  2:3258709/1‑148 (MQ=255)
                                                cGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTa                                                                                                  >  2:1619766/1‑148 (MQ=255)
                                                  tATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAac                                                                                                <  1:760317/148‑1 (MQ=255)
                                                  tATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAac                                                                                                >  1:578718/1‑148 (MQ=255)
                                                    tctcGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAacac                                                                                              <  2:1407300/148‑1 (MQ=255)
                                                     ctcGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACAcc                                                                                             >  2:3238814/1‑148 (MQ=255)
                                                      tcGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCTATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCt                                                                                            <  2:892502/148‑1 (MQ=255)
                                                           tAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCt                                                                                       <  2:138546/148‑1 (MQ=255)
                                                             aaGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTtc                                                                                     >  2:209877/1‑148 (MQ=255)
                                                             aaGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTtc                                                                                     >  2:3028142/1‑148 (MQ=255)
                                                               gATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTtctc                                                                                   <  2:374985/148‑1 (MQ=255)
                                                                aTACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTcc                                                                                  >  2:1177816/1‑148 (MQ=255)
                                                                aTACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTcc                                                                                  >  2:265869/1‑148 (MQ=255)
                                                                           aTTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGc                                                                       >  1:2550898/1‑148 (MQ=255)
                                                                               tCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGa                                                                   >  1:380586/1‑148 (MQ=255)
                                                                                cGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACACTGCCGATGTAAAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGaa                                                                  >  1:3249882/1‑148 (MQ=255)
                                                                                  aCGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGaaaa                                                                <  2:2017132/148‑1 (MQ=255)
                                                                                  aCGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGaaaa                                                                <  2:1255683/148‑1 (MQ=255)
                                                                                  aCGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGaaaa                                                                <  2:1058984/148‑1 (MQ=255)
                                                                                   cGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGaaaaa                                                               >  1:242849/1‑148 (MQ=255)
                                                                                   cGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGaaaaa                                                               <  1:2326415/148‑1 (MQ=255)
                                                                                     ccATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGc                                                             <  2:575193/148‑1 (MQ=255)
                                                                                       aTCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCg                                                           >  1:3057221/1‑148 (MQ=255)
                                                                                        tCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGa                                                          >  1:1370553/1‑148 (MQ=255)
                                                                                        tCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACAGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGa                                                          >  2:1882600/1‑148 (MQ=255)
                                                                                           tCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAAc                                                       >  2:1272052/1‑148 (MQ=255)
                                                                                           tCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAAc                                                       >  1:3100303/1‑148 (MQ=255)
                                                                                            cAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAAcc                                                      <  1:2397968/148‑1 (MQ=255)
                                                                                            cAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAAcc                                                      >  1:2706500/1‑148 (MQ=255)
                                                                                            cAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAAcc                                                      <  1:2397718/148‑1 (MQ=255)
                                                                                               cGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTg                                                   <  2:1018329/148‑1 (MQ=255)
                                                                                                  ttCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCTATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGCCg                                                >  2:2137021/1‑148 (MQ=255)
                                                                                                  ttCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCg                                                >  2:1912972/1‑148 (MQ=255)
                                                                                                   tCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGa                                               >  1:607255/1‑148 (MQ=255)
                                                                                                   tCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGa                                               >  1:607137/1‑148 (MQ=255)
                                                                                                     gCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCTATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGaaa                                             <  2:296550/148‑1 (MQ=255)
                                                                                                        ggATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTg                                          >  1:1496656/1‑148 (MQ=255)
                                                                                                        ggATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTg                                          >  1:922465/1‑148 (MQ=255)
                                                                                                         gATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGt                                         >  2:1431438/1‑148 (MQ=255)
                                                                                                          aTAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGtt                                        >  2:888460/1‑148 (MQ=255)
                                                                                                          aTAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGtt                                        >  1:1459118/1‑148 (MQ=255)
                                                                                                           tAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGttt                                       <  2:462519/148‑1 (MQ=255)
                                                                                                              tGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATc                                    >  1:386990/1‑148 (MQ=255)
                                                                                                                cAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGa                                  >  2:2537190/1‑148 (MQ=255)
                                                                                                                  gAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACa                                <  2:221016/148‑1 (MQ=255)
                                                                                                                   aaaCCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACaa                               >  1:1832399/1‑148 (MQ=255)
                                                                                                                    aaCCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAAc                              <  1:101507/148‑1 (MQ=255)
                                                                                                                      ccTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACtt                            >  1:1337188/1‑148 (MQ=255)
                                                                                                                      ccTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACtt                            >  1:2297385/1‑148 (MQ=255)
                                                                                                                         tCACTCTGCCGATGTTTAACCTGGCTATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGa                         >  2:3086941/1‑148 (MQ=255)
                                                                                                                         tCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGACACCCTGGCGAAACTGTTTATCGACAACTTCGa                         >  2:473337/1‑148 (MQ=255)
                                                                                                                         tCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGa                         >  1:536343/1‑148 (MQ=255)
                                                                                                                              ctGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAAt                    >  1:2814024/1‑148 (MQ=255)
                                                                                                                              ctGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAAt                    <  1:1173644/148‑1 (MQ=255)
                                                                                                                               tGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATa                   <  1:2789277/148‑1 (MQ=255)
                                                                                                                                 ccGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATaca                 <  1:2132767/148‑1 (MQ=255)
                                                                                                                                    aTGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCg              >  2:1196203/1‑148 (MQ=255)
                                                                                                                                    aTGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCg              >  2:1520448/1‑148 (MQ=255)
                                                                                                                                      gTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGac            >  2:15088/1‑148 (MQ=255)
                                                                                                                                       tttAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGaca           >  1:423853/1‑148 (MQ=255)
                                                                                                                                        ttAACCTGGCTATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGacac          >  1:862374/1‑148 (MQ=255)
                                                                                                                                        ttAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGacac          >  2:708428/1‑148 (MQ=255)
                                                                                                                                           aCCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACAcccc       <  2:2202224/148‑1 (MQ=255)
                                                                                                                                            ccTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCt      >  1:2604061/1‑148 (MQ=255)
                                                                                                                                            ccTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCt      >  1:2601211/1‑148 (MQ=255)
                                                                                                                                              tGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGc    >  2:209144/1‑148 (MQ=255)
                                                                                                                                               ggCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGCg   >  1:2732135/1‑148 (MQ=255)
                                                                                                                                                gCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGCgg  >  1:143686/1‑148 (MQ=255)
                                                                                                                                                gCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGCgg  >  2:2258866/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGCGCGCAGGCTTATCTGGTTAACACTGGCTGGAACGGCACTGGCAAACGTATCTCGATTAAAGATACCCGCGCCATTATCGACGCCATCCTCAACGGTTCGCTGGATAATGCAGAAACCTTCACTCTGCCGATGTTTAACCTGGCGATCCCAACCGAACTGCCGGGCGTAGACACGAAGATTCTCGATCCGCGTAACACCTACGCTTCTCCGGAACAGTGGCAGGAAAAAGCCGAAACCCTGGCGAAACTGTTTATCGACAACTTCGATAAATACACCGACACCCCTGCGG  >  CP009273/3527473‑3527764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑