Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,543,959 G→T 100% A625E (GCG→GAG)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,543,9590GT99.4% 492.0 / ‑5.8 164A625E (GCG→GAG) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base T (80/81);  minor base A (2/0);  total (83/81)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 174 total aligned reads displayed.

ATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC  >  CP009273/3543814‑3544101
                                                                                                                                                 |                                                                                                                                              
atTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGNGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctcc                                                                                                                                              >  1:1676637/1‑148 (MQ=255)
atTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctcc                                                                                                                                              >  1:1103366/1‑148 (MQ=255)
atTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctcc                                                                                                                                              >  1:1580486/1‑148 (MQ=255)
atTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATacgcctcc                                                                                                                                              >  1:2083347/1‑148 (MQ=255)
 ttttCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctccg                                                                                                                                             >  2:294391/1‑148 (MQ=255)
 ttttCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctccg                                                                                                                                             <  2:1102088/148‑1 (MQ=255)
    tcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGa                                                                                                                                          >  1:2539653/1‑148 (MQ=255)
    tcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGa                                                                                                                                          >  1:2539662/1‑148 (MQ=255)
      ttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATc                                                                                                                                        >  2:3104823/1‑148 (MQ=255)
      ttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATc                                                                                                                                        >  2:3104798/1‑148 (MQ=255)
       tcACCGACTTTCTCGGCGATTTCATCGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCATTACCTGCAGTAGAAATTTGTACGGAGATAACCGCCTCCGGGATCa                                                                                                                                       >  1:819392/1‑148 (MQ=255)
       tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTTCCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGATCa                                                                                                                                       >  1:844716/1‑148 (MQ=255)
       tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGCAATTTGTTCGGAGATATCCGCCTCCGGGATCa                                                                                                                                       >  2:1546776/1‑148 (MQ=255)
       tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGAACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTATTTACCTGCATTCGAAATTTGTTCGGAGATATACGCCTCCGGGATCa                                                                                                                                       >  2:850064/1‑148 (MQ=255)
            gACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtttt                                                                                                                                  <  1:40874/148‑1 (MQ=255)
            gACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtttt                                                                                                                                  <  1:446894/148‑1 (MQ=255)
             aCTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttttt                                                                                                                                 <  1:754537/148‑1 (MQ=255)
             aCTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttttt                                                                                                                                 >  2:659610/1‑148 (MQ=255)
             aCTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttttt                                                                                                                                 >  1:785872/1‑148 (MQ=255)
             aCTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttttt                                                                                                                                 >  1:785823/1‑148 (MQ=255)
                 tctcGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgc                                                                                                                             <  2:1469358/148‑1 (MQ=255)
                 tctcGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgc                                                                                                                             <  1:1369629/148‑1 (MQ=255)
                    cGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTTCCTGCAGTCGAAATTTTTTCGGAGATATACGCCGCCGGGATCAGTTTTTacgccgc                                                                                                                          >  1:2780638/1‑148 (MQ=255)
                     ggCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgccgcc                                                                                                                         >  1:2859321/1‑148 (MQ=255)
                     ggCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGATCAGTTTTTccgccgcc                                                                                                                         >  1:596015/1‑148 (MQ=255)
                        gATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGaa                                                                                                                      <  1:688366/148‑1 (MQ=255)
                        gATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGaa                                                                                                                      <  1:1007064/148‑1 (MQ=255)
                        gATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGaa                                                                                                                      <  1:2349621/148‑1 (MQ=255)
                          tttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAc                                                                                                                    >  2:1013051/1‑148 (MQ=255)
                          tttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAc                                                                                                                    >  2:1190060/1‑148 (MQ=255)
                          tttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTAGGACATATCCGCCTCCGGGATCAGTTTTTCCCCCGCCGaaaa                                                                                                                    >  1:826948/1‑147 (MQ=255)
                           ttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGCTCAGTTTTTCCGCCGCCGAAACg                                                                                                                   >  1:678345/1‑148 (MQ=255)
                           ttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACg                                                                                                                   >  1:1301723/1‑148 (MQ=255)
                             cAACGTTCGCCCCATCCAGAGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCa                                                                                                                 <  1:2101086/148‑1 (MQ=255)
                              aaCGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaa                                                                                                                <  2:560800/148‑1 (MQ=255)
                               aCGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTATTTACCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGGTCAGTTTTTACGCCGCCGCAACGCaat                                                                                                               >  1:319961/1‑148 (MQ=255)
                                  ttCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaataat                                                                                                            <  1:1085144/148‑1 (MQ=255)
                                   tCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCACTACTc                                                                                                           >  2:2858179/1‑148 (MQ=255)
                                   tCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTAGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCACTAATc                                                                                                           >  2:2771864/1‑148 (MQ=255)
                                    cGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATcc                                                                                                          <  2:2544296/148‑1 (MQ=255)
                                        ccATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCa                                                                                                      >  2:2993622/1‑148 (MQ=255)
                                         cATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAg                                                                                                     <  2:1442625/148‑1 (MQ=255)
                                          aTCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAgg                                                                                                    >  1:2975442/1‑148 (MQ=255)
                                           tCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCACTCGAAATTTGATGGGAGATATCCGACTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAAAACGGCAGGa                                                                                                   >  2:2057997/1‑148 (MQ=255)
                                            ccAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGaa                                                                                                  >  2:1380281/1‑148 (MQ=255)
                                             cAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAac                                                                                                 <  2:1690675/148‑1 (MQ=255)
                                              aGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAaca                                                                                                <  1:1387168/148‑1 (MQ=255)
                                                  tACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAAcaccac                                                                                            >  2:1782327/1‑148 (MQ=255)
                                                  tACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAAcaccac                                                                                            <  1:1272827/148‑1 (MQ=255)
                                                   aCCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACaccacc                                                                                           <  1:887209/148‑1 (MQ=255)
                                                    ccGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCt                                                                                          >  1:924201/1‑148 (MQ=255)
                                                        cAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCaa                                                                                      <  2:793005/148‑1 (MQ=255)
                                                        cAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCGTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCACCGGGATCAGTTTTTCCGCCGCCGCAACGCAATAATCCGGCAGGAACACCACCATCaa                                                                                      >  1:22776/1‑148 (MQ=255)
                                                           tAAGCGCACCATTGAGCGCCAGTTTCATATTGACGGTACCGGAAGCTTCTTTAACTGCAGTCGAAATTTTTTCGGAGATATACGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAAAAATACAGCAGGAACAAAACCTTCAAcat                                                                                   >  1:2064764/1‑146 (MQ=255)
                                                              gcgcACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATc                                                                                >  1:3017170/1‑148 (MQ=255)
                                                              gcgcACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATc                                                                                >  2:1871727/1‑148 (MQ=255)
                                                                       tGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAg                                                                       <  2:1581220/148‑1 (MQ=255)
                                                                       tGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAg                                                                       <  1:1013051/148‑1 (MQ=255)
                                                                           cgcCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGa                                                                   <  2:1314807/148‑1 (MQ=255)
                                                                                 tttCATAGTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgttg                                                             <  2:2855978/148‑1 (MQ=255)
                                                                                     atttttccGGTACCGGAAGCTTCTTTACCTTCAGTCGAAATTTGTTCGGAGATTTCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAAATTATCGCCAACCAGCGGATCGTTGTTGa                                                         <  1:1481270/142‑1 (MQ=255)
                                                                                      tatTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGAt                                                        <  2:2260090/148‑1 (MQ=255)
                                                                                       atTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATc                                                       >  2:1536496/1‑148 (MQ=255)
                                                                                         tGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGCACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCAc                                                     >  2:2230776/1‑148 (MQ=255)
                                                                                          gCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACg                                                    <  1:2747099/148‑1 (MQ=255)
                                                                                                   ggAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACt                                           >  2:3195992/1‑148 (MQ=255)
                                                                                                      aGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttg                                        <  2:603105/148‑1 (MQ=255)
                                                                                                       gCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgt                                       <  2:821867/148‑1 (MQ=255)
                                                                                                       gCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgt                                       <  1:1931196/148‑1 (MQ=255)
                                                                                                       gCTTCTTGACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgt                                       <  2:2072883/148‑1 (MQ=255)
                                                                                                       gCTTCTGTACGTGCAGTCGAAATTTGTTCGGAGTTATCAGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACGTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgt                                       <  2:819392/148‑1 (MQ=255)
                                                                                                          tcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGa                                    <  2:1974298/148‑1 (MQ=255)
                                                                                                           cttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGTATCAGTTTTTCCGCCGCCGTATCGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGACACTTTGTTGAt                                   <  1:2057997/148‑1 (MQ=255)
                                                                                                           cttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGAt                                   <  2:2486476/148‑1 (MQ=255)
                                                                                                           cttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGAt                                   <  1:1538588/148‑1 (MQ=255)
                                                                                                                 cTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCaca                             >  2:2654666/1‑146 (MQ=255)
                                                                                                                     aGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGata                         <  1:1380281/148‑1 (MQ=255)
                                                                                                                      gTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataa                        <  2:1425928/148‑1 (MQ=255)
                                                                                                                       tCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                       <  2:2083347/148‑1 (MQ=255)
                                                                                                                          aaaTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAtt                    <  1:1782327/148‑1 (MQ=255)
                                                                                                                          aaaTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAtt                    <  2:345038/148‑1 (MQ=255)
                                                                                                                            aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATACTAttct                  >  2:901877/1‑148 (MQ=255)
                                                                                                                            aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttct                  >  2:2594823/1‑148 (MQ=255)
                                                                                                                            aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttct                  >  2:2594831/1‑148 (MQ=255)
                                                                                                                            aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttct                  <  2:1933395/148‑1 (MQ=255)
                                                                                                                            aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttct                  <  2:2064826/148‑1 (MQ=255)
                                                                                                                             tttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttctt                 >  2:1290463/1‑148 (MQ=255)
                                                                                                                             tttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttctt                 >  1:1737121/1‑148 (MQ=255)
                                                                                                                              ttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttcttc                >  1:391377/1‑148 (MQ=255)
                                                                                                                              ttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttcttc                >  2:1069695/1‑148 (MQ=255)
                                                                                                                                 ttCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGcc             <  1:2664351/148‑1 (MQ=255)
                                                                                                                                 ttCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGcc             >  1:3070392/1‑148 (MQ=255)
                                                                                                                                     gagaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgt         >  1:1665177/1‑148 (MQ=255)
                                                                                                                                      agaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgta        >  1:2716905/1‑148 (MQ=255)
                                                                                                                                      agaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgta        >  1:2271661/1‑148 (MQ=255)
                                                                                                                                       gaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtag       <  2:1617080/148‑1 (MQ=255)
                                                                                                                                       gaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtag       <  2:1617076/148‑1 (MQ=255)
                                                                                                                                        atatCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtagt      >  1:1059528/1‑148 (MQ=255)
                                                                                                                                         tatCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtagta     <  1:2014397/148‑1 (MQ=255)
                                                                                                                                            ccgcctccgGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGcc  <  1:3228153/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
ATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC  >  CP009273/3543814‑3544101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑