Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,544,164 G→T 11.4% R557S (CGT→AGT)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,544,1640GT11.4% 697.8 / 44.0 215R557S (CGT→AGT) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (101/89);  new base T (9/16);  total (110/105)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 217 total aligned reads displayed.

TATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACGGACTTT  >  CP009273/3544020‑3544311
                                                                                                                                                |                                                                                                                                                   
tATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAAt                                                                                                                                                  >  1:1508906/1‑148 (MQ=255)
tATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAAt                                                                                                                                                  <  2:2266869/148‑1 (MQ=255)
tATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAAt                                                                                                                                                  >  2:173252/1‑148 (MQ=255)
  tCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAAttt                                                                                                                                                <  1:1608503/148‑1 (MQ=255)
      cAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCttt                                                                                                                                            <  2:22776/148‑1 (MQ=255)
       aaCCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTg                                                                                                                                           <  2:2716905/148‑1 (MQ=255)
        aCCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGt                                                                                                                                          <  2:2038504/148‑1 (MQ=255)
         ccAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTa                                                                                                                                         <  1:601843/148‑1 (MQ=255)
           aGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACa                                                                                                                                       >  2:1914651/1‑148 (MQ=255)
           aGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACa                                                                                                                                       <  1:1671563/148‑1 (MQ=255)
           aGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCACAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACa                                                                                                                                       <  1:318666/148‑1 (MQ=255)
           aGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCACAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACa                                                                                                                                       <  1:1389686/148‑1 (MQ=255)
            gCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACaa                                                                                                                                      <  1:1975516/148‑1 (MQ=255)
                 tCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCa                                                                                                                                 >  1:1902099/1‑148 (MQ=255)
                  cGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGTGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAg                                                                                                                                >  1:806042/1‑148 (MQ=255)
                  cGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAg                                                                                                                                >  2:263717/1‑148 (MQ=255)
                  cGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAg                                                                                                                                >  1:2757454/1‑148 (MQ=255)
                       ttgATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAatat                                                                                                                           <  1:2774032/148‑1 (MQ=255)
                         gATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATgc                                                                                                                         <  2:785872/148‑1 (MQ=255)
                          aTCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATgca                                                                                                                        >  1:1778951/1‑148 (MQ=255)
                          aTCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATgca                                                                                                                        <  1:783255/148‑1 (MQ=255)
                            cACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCCGAATCTgcagc                                                                                                                      >  2:2256113/1‑148 (MQ=255)
                            cACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATgcagc                                                                                                                      >  1:2492210/1‑148 (MQ=255)
                             aCGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATgcagca                                                                                                                     >  1:1337446/1‑148 (MQ=255)
                                 cAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGAtt                                                                                                                 >  1:1400860/1‑148 (MQ=255)
                                  aGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTc                                                                                                                <  2:924201/148‑1 (MQ=255)
                                   gCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCa                                                                                                               >  2:1499570/1‑148 (MQ=255)
                                   gCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCa                                                                                                               >  1:1656467/1‑148 (MQ=255)
                                   gCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCa                                                                                                               >  2:2761928/1‑148 (MQ=255)
                                   gCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCa                                                                                                               >  2:3140232/1‑148 (MQ=255)
                                    ccACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAg                                                                                                              >  1:968348/1‑148 (MQ=255)
                                    ccACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAg                                                                                                              >  1:2619994/1‑148 (MQ=255)
                                      aCTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTAAGGt                                                                                                            >  2:1126673/1‑148 (MQ=255)
                                       cTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCGCGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTg                                                                                                           >  1:2540070/1‑148 (MQ=255)
                                       cTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTg                                                                                                           >  1:2179379/1‑148 (MQ=255)
                                            ttgATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGc                                                                                                      >  2:2886381/1‑148 (MQ=255)
                                               aTCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGtt                                                                                                   <  1:2274934/148‑1 (MQ=255)
                                                tCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGttt                                                                                                  >  2:1016672/1‑148 (MQ=255)
                                                tCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGttt                                                                                                  >  1:829902/1‑148 (MQ=255)
                                                tCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGttt                                                                                                  >  1:665944/1‑148 (MQ=255)
                                                 cGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTg                                                                                                 <  1:2666093/148‑1 (MQ=255)
                                                  gCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGt                                                                                                <  2:319969/148‑1 (MQ=255)
                                                  gCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGt                                                                                                <  1:1290463/148‑1 (MQ=255)
                                                   cAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTa                                                                                               <  2:391377/148‑1 (MQ=255)
                                                   cAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGtt                                                                                               >  1:2065970/1‑147 (MQ=255)
                                                   cAAAGATAATATTCGTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTa                                                                                               <  2:3070392/148‑1 (MQ=255)
                                                   cAAAGATAATAGTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTa                                                                                               <  1:2230776/148‑1 (MQ=255)
                                                     aaGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACt                                                                                             >  1:3202723/1‑148 (MQ=255)
                                                      aGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTc                                                                                            <  2:2539653/148‑1 (MQ=255)
                                                       gATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCa                                                                                           <  2:522804/148‑1 (MQ=255)
                                                       gATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCACCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCa                                                                                           >  2:1176048/1‑148 (MQ=255)
                                                         taataTTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATg                                                                                         >  2:1064331/1‑148 (MQ=255)
                                                         taataTTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATg                                                                                         >  2:3124779/1‑148 (MQ=255)
                                                         taataTTCTTCGCCAGGTAG‑AGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTTCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGc                                                                                        >  2:47813/1‑148 (MQ=255)
                                                            tatTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCaa                                                                                      <  1:1069690/148‑1 (MQ=255)
                                                             atTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATTCaaa                                                                                     >  1:855631/1‑148 (MQ=255)
                                                             atTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCaaa                                                                                     >  2:914495/1‑148 (MQ=255)
                                                              ttcttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCacac                                                                                    >  1:693021/1‑148 (MQ=255)
                                                              ttcttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAAc                                                                                    >  2:2751502/1‑148 (MQ=255)
                                                              ttcttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAAc                                                                                    >  2:2908845/1‑148 (MQ=255)
                                                              ttcttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAAc                                                                                    >  2:2932862/1‑148 (MQ=255)
                                                              ttcttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAAc                                                                                    >  1:2805579/1‑148 (MQ=255)
                                                                cttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGt                                                                                  <  1:3104823/148‑1 (MQ=255)
                                                                 ttcGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACggt                                                                                 >  1:1570940/1‑146 (MQ=255)
                                                                    gCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGa                                                                              >  2:1395944/1‑148 (MQ=255)
                                                                       aGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTTCGCATGCAAAAGTTTGAtat                                                                           >  1:32658/1‑146 (MQ=255)
                                                                       aGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCt                                                                           <  2:1665177/148‑1 (MQ=255)
                                                                       aGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCt                                                                           <  2:738993/148‑1 (MQ=255)
                                                                       aGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTGTGTACTCATGCAAAACTTTGAtat                                                                           >  1:1464410/1‑146 (MQ=255)
                                                                         gtagtaGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGa                                                                         <  1:2771864/148‑1 (MQ=255)
                                                                         gtagtaGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGa                                                                         >  1:416063/1‑148 (MQ=255)
                                                                          tagtagCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCTGGTTTTCACGAATTTCTTTGTACAACGACAGAATATGAAGCAGATTCAGGTGCTGGCGTTTTTACTAATTAAAACGTTTGATCTGaa                                                                        >  2:2244628/1‑148 (MQ=255)
                                                                          tagtagCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGaa                                                                        <  2:2859321/148‑1 (MQ=255)
                                                                          tagtagCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGaa                                                                        >  2:844485/1‑148 (MQ=255)
                                                                          tagtagCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGaa                                                                        <  2:596015/148‑1 (MQ=255)
                                                                                          cTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCCTGCAAACGTTTGATCTGAATATCGAAAATCGCCtg                                                        >  2:80192/1‑148 (MQ=255)
                                                                                            ttCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTGTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCggtg                                                      >  2:2814161/1‑148 (MQ=255)
                                                                                             tCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTgg                                                     <  1:2302477/148‑1 (MQ=255)
                                                                                                 gcCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTg                                                 <  2:1345915/148‑1 (MQ=255)
                                                                                                   cGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCGCGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGAt                                               <  2:2540070/148‑1 (MQ=255)
                                                                                                   cGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTgtt                                               >  1:127138/1‑146 (MQ=255)
                                                                                                    gAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGAtc                                              <  2:1676637/148‑1 (MQ=255)
                                                                                                       gagGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCa                                           <  2:1737121/148‑1 (MQ=255)
                                                                                                         ggAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAAt                                         <  2:1299219/148‑1 (MQ=255)
                                                                                                         ggAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAAt                                         >  1:1931168/1‑148 (MQ=255)
                                                                                                          gAAGACGCGCGGTACGCGATCAGCCTGCGTGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCTTTTGTACTCATGCAAACTTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATa                                        <  1:3068558/148‑1 (MQ=255)
                                                                                                               cgcgcgGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGt                                   <  2:1963661/148‑1 (MQ=255)
                                                                                                               cgcgcgGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTGTGTACTCCTGCAAACGGTTTATCAGAATATCGAAAATCGCCTGTGGATTGATCTAAATAACGGt                                   >  1:2839044/1‑148 (MQ=255)
                                                                                                                gcgcgGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTa                                  <  1:2864296/148‑1 (MQ=255)
                                                                                                                       aCGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTGTGGACGCATGCAAACGGTTGTTCTGAATATCGAAAATCGCCTGTGGATTGATCTCAAGACCGGTCCGAACtt                           >  1:1218139/1‑148 (MQ=255)
                                                                                                                         gcgATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTc                         <  2:2911163/148‑1 (MQ=255)
                                                                                                                              cAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCCAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACaaa                    >  2:18828/1‑148 (MQ=255)
                                                                                                                               aGCCTGCGGGTTTTCACTAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAAc                   <  2:1336600/148‑1 (MQ=255)
                                                                                                                                gCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTTATCTCAATACCGGTACGAACTTTCACAAACt                  >  1:1852497/1‑148 (MQ=255)
                                                                                                                                gCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACt                  <  1:181555/148‑1 (MQ=255)
                                                                                                                                        tttttCACTAATTTCTTTTTACAACGCCATAATATGCAGCAGTTTCTGGTGGTGGCTTTTGTACTCATGCAAAGGTTTGATCTGAATATCCAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGa          <  1:1126673/147‑1 (MQ=255)
                                                                                                                                        gTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGCTTGATCTCAATACCGGTACGAACTTTCACAAAATCCGCCaca          >  1:1714234/1‑146 (MQ=255)
                                                                                                                                            tCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACGGa      <  2:1508906/148‑1 (MQ=255)
                                                                                                                                             cACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACGGAc     <  1:914495/148‑1 (MQ=255)
                                                                                                                                                gAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACGGACttt  <  2:1845980/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCCGCTTTCGCGCCGAAGAGGAAGACGCGCGGTACGCGATCAGCCTGCGGGTTTTCACGAATTTCTTTGTACAACGCCAGAATATGCAGCAGATTCAGGTGCTGGCGTTTGTACTCATGCAAACGTTTGATCTGAATATCGAAAATCGCCTGTGGATTGATCTCAATACCGGTACGAACTTTCACAAACTCCGCCAGACGGACTTT  >  CP009273/3544020‑3544311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑