Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,549,090 C→A 7.4% R883S (CGC→AGC)  malT → mal regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,549,0900CA 7.4% 692.6 / 20.7 187R883S (CGC→AGC) malTmal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (66/107);  new base A (7/7);  total (73/114)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 188 total aligned reads displayed.

GCCCGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTA  >  CP009273/3548944‑3549237
                                                                                                                                                  |                                                                                                                                                   
gCCCGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCg                                                                                                                                                    >  1:2483533/1‑148 (MQ=255)
 cccGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGc                                                                                                                                                   >  2:1906527/1‑148 (MQ=255)
 cccGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGc                                                                                                                                                   <  2:2920682/148‑1 (MQ=255)
  ccGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGcc                                                                                                                                                  <  2:3122319/148‑1 (MQ=255)
   cGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCa                                                                                                                                                 <  1:2120697/148‑1 (MQ=255)
     cTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAgg                                                                                                                                               <  1:416684/148‑1 (MQ=255)
      tGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGa                                                                                                                                              <  2:2023261/148‑1 (MQ=255)
       gACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGAt                                                                                                                                             >  1:2261656/1‑148 (MQ=255)
        aCGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCACAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATg                                                                                                                                            <  2:2245145/148‑1 (MQ=255)
        aCGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATg                                                                                                                                            >  1:2339666/1‑148 (MQ=255)
           cAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCgg                                                                                                                                         <  2:1238275/148‑1 (MQ=255)
           cAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCgg                                                                                                                                         <  1:1927201/148‑1 (MQ=255)
              cGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTac                                                                                                                                      >  1:2355902/1‑148 (MQ=255)
              cGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTac                                                                                                                                      >  2:3109712/1‑148 (MQ=255)
                tGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTacaa                                                                                                                                    <  1:2796461/148‑1 (MQ=255)
                 gAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTacaac                                                                                                                                   >  2:1012880/1‑148 (MQ=255)
                 gAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTacaac                                                                                                                                   >  2:1901612/1‑148 (MQ=255)
                  aaTGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTacaaca                                                                                                                                  >  1:1493957/1‑148 (MQ=255)
                    tGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACg                                                                                                                                <  1:2010627/148‑1 (MQ=255)
                           tACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCa                                                                                                                         <  2:217696/148‑1 (MQ=255)
                             cTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAAt                                                                                                                       <  1:2790279/148‑1 (MQ=255)
                             cTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAAt                                                                                                                       <  1:955468/148‑1 (MQ=255)
                                gggCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGc                                                                                                                    <  2:180541/148‑1 (MQ=255)
                                  gCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTg                                                                                                                  >  2:1544446/1‑148 (MQ=255)
                                   cTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGa                                                                                                                 <  1:1981636/148‑1 (MQ=255)
                                   cTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGa                                                                                                                 <  1:2823588/148‑1 (MQ=255)
                                    tGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGaa                                                                                                                >  1:1418792/1‑148 (MQ=255)
                                          aCTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACTCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAgatgat                                                                                                          <  2:620233/148‑1 (MQ=255)
                                          aCTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAgatgat                                                                                                          >  2:1062998/1‑148 (MQ=255)
                                             ctGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATggg                                                                                                       >  1:625205/1‑148 (MQ=255)
                                              tGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGTGGTACAACACGCCCAGCAATTGCTGAAGATGATgggg                                                                                                      <  2:458303/148‑1 (MQ=255)
                                              tGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATgggg                                                                                                      <  2:2332158/148‑1 (MQ=255)
                                                  tACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTAGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACg                                                                                                  >  2:1680090/1‑148 (MQ=255)
                                                   aCAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACgg                                                                                                 >  2:1359534/1‑148 (MQ=255)
                                                   aCAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACgg                                                                                                 <  1:642482/148‑1 (MQ=255)
                                                       cAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCgtg                                                                                             <  1:1370888/148‑1 (MQ=255)
                                                         aTGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTa                                                                                           >  2:2816964/1‑148 (MQ=255)
                                                          tGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTaa                                                                                          >  1:456593/1‑148 (MQ=255)
                                                           gAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAACACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAg                                                                                         <  1:1714503/148‑1 (MQ=255)
                                                            aGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGt                                                                                        <  1:824633/148‑1 (MQ=255)
                                                            aGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGt                                                                                        <  1:3162064/148‑1 (MQ=255)
                                                               aaaTTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTa                                                                                     <  1:651132/148‑1 (MQ=255)
                                                                 aTTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGc                                                                                   >  1:1356226/1‑148 (MQ=255)
                                                                 aTTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGc                                                                                   >  1:2999396/1‑148 (MQ=255)
                                                                   tGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCg                                                                                 <  1:3287795/148‑1 (MQ=255)
                                                                    gCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCgg                                                                                <  1:1329328/148‑1 (MQ=255)
                                                                     ccGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGa                                                                               <  2:709875/148‑1 (MQ=255)
                                                                       ggAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATa                                                                             <  2:555253/148‑1 (MQ=255)
                                                                        gagaACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATaa                                                                            >  2:2371579/1‑148 (MQ=255)
                                                                        gagaACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATaa                                                                            >  2:2427795/1‑148 (MQ=255)
                                                                        gagaACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATaa                                                                            >  2:512761/1‑148 (MQ=255)
                                                                         agaACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAAc                                                                           >  1:1069467/1‑148 (MQ=255)
                                                                          gaACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAAcg                                                                          <  2:2504075/148‑1 (MQ=255)
                                                                           aaCTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAAcgc                                                                         >  2:2252828/1‑148 (MQ=255)
                                                                           aaCTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAAcgc                                                                         >  2:2558080/1‑148 (MQ=255)
                                                                                 aaGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGa                                                                   >  2:1195556/1‑148 (MQ=255)
                                                                                 aaGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGa                                                                   <  1:127991/148‑1 (MQ=255)
                                                                                  aGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGAt                                                                  >  2:1351399/1‑148 (MQ=255)
                                                                                  aGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGAt                                                                  <  2:2540840/148‑1 (MQ=255)
                                                                                        ggCAACCACCATCACAACCCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCt                                                            <  2:413466/148‑1 (MQ=255)
                                                                                          cAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTa                                                          <  2:1347688/148‑1 (MQ=255)
                                                                                          cAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTa                                                          <  2:1769761/148‑1 (MQ=255)
                                                                                          cAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTa                                                          <  2:1347795/148‑1 (MQ=255)
                                                                                             ccaccaTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCACTTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACAt                                                       >  2:1964100/1‑148 (MQ=255)
                                                                                              caccaTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACAtc                                                      <  1:1012880/148‑1 (MQ=255)
                                                                                               accaTCAAACCGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACAtct                                                     <  1:2252503/148‑1 (MQ=255)
                                                                                               accaTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACAtct                                                     <  2:2042361/148‑1 (MQ=255)
                                                                                               accaTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACAtct                                                     <  2:2042367/148‑1 (MQ=255)
                                                                                                ccaTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACAtctc                                                    <  1:1359741/148‑1 (MQ=255)
                                                                                                      aaaCGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGcatc                                              <  2:3261648/148‑1 (MQ=255)
                                                                                                       aaCGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGcatca                                             <  2:751536/148‑1 (MQ=255)
                                                                                                           cATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCa                                         <  2:1371303/148‑1 (MQ=255)
                                                                                                           cATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCa                                         >  1:652738/1‑148 (MQ=255)
                                                                                                           cATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCa                                         >  1:2700701/1‑148 (MQ=255)
                                                                                                            atatCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCaa                                        >  2:2480368/1‑148 (MQ=255)
                                                                                                                 cGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTc                                   <  2:1114443/148‑1 (MQ=255)
                                                                                                                   cAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCAc                                 >  1:2594643/1‑148 (MQ=255)
                                                                                                                   cAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCAc                                 >  1:343087/1‑148 (MQ=255)
                                                                                                                        tGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCcgcg                            <  2:2813058/148‑1 (MQ=255)
                                                                                                                         gTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGt                           >  2:52825/1‑148 (MQ=255)
                                                                                                                         gTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGt                           >  1:2319936/1‑148 (MQ=255)
                                                                                                                                aaaCTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCa                    >  2:2663018/1‑148 (MQ=255)
                                                                                                                                 aaCTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCAGTCAATGCTCACCCGCGTTACGCCAt                   <  2:325919/148‑1 (MQ=255)
                                                                                                                                  aCTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATc                  <  2:914086/148‑1 (MQ=255)
                                                                                                                                  aCTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATc                  <  2:198661/148‑1 (MQ=255)
                                                                                                                                  aCTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATc                  <  1:3185692/148‑1 (MQ=255)
                                                                                                                                    tCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTg                >  2:2968981/1‑148 (MQ=255)
                                                                                                                                    tCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTg                >  2:2969024/1‑148 (MQ=255)
                                                                                                                                    tCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTg                >  1:713950/1‑148 (MQ=255)
                                                                                                                                    tCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTg                >  1:713923/1‑148 (MQ=255)
                                                                                                                                       gCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGttt             >  1:400225/1‑148 (MQ=255)
                                                                                                                                       gCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGttt             <  2:3030988/148‑1 (MQ=255)
                                                                                                                                         gTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCt           <  2:2588290/148‑1 (MQ=255)
                                                                                                                                         gTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCt           <  2:681886/148‑1 (MQ=255)
                                                                                                                                            gCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATc        <  1:3232073/148‑1 (MQ=255)
                                                                                                                                              ccATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCaa      <  2:1630252/148‑1 (MQ=255)
                                                                                                                                              ccATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACGTCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGGTTCTATCaa      >  2:1785492/1‑148 (MQ=255)
                                                                                                                                               cATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCaaa     <  1:2668852/148‑1 (MQ=255)
                                                                                                                                               cATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCaaa     >  1:1784153/1‑148 (MQ=255)
                                                                                                                                               cATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCaaa     <  1:1671159/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCCCGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTA  >  CP009273/3548944‑3549237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑