Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,582,807 G→T 90.9% P194Q (CCG→CAG)  ugpE ← glycerol‑3‑phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,582,8070GT90.9% 507.4 / 39.3 190P194Q (CCG→CAG) ugpEglycerol‑3‑phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (8/10);  new base T (91/81);  total (99/91)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

TTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGG  >  CP009273/3582662‑3582951
                                                                                                                                                 |                                                                                                                                                
ttCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGa                                                                                                                                                >  2:3289648/1‑148 (MQ=255)
 tCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGaa                                                                                                                                               >  2:2010718/1‑148 (MQ=255)
   gCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGaaaa                                                                                                                                             <  2:1215894/148‑1 (MQ=255)
      tGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACg                                                                                                                                          >  2:839867/1‑148 (MQ=255)
      tGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACg                                                                                                                                          <  1:2955945/148‑1 (MQ=255)
      tGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACg                                                                                                                                          <  1:2999041/148‑1 (MQ=255)
      tGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACg                                                                                                                                          <  1:1592733/148‑1 (MQ=255)
       gTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGa                                                                                                                                         >  1:794819/1‑148 (MQ=255)
       gTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGa                                                                                                                                         >  1:795192/1‑148 (MQ=255)
        tAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGTTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGAt                                                                                                                                        <  1:2348344/148‑1 (MQ=255)
          gCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGt                                                                                                                                      <  2:3080200/148‑1 (MQ=255)
          gCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGt                                                                                                                                      <  1:2172218/148‑1 (MQ=255)
            gATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCg                                                                                                                                    <  1:1772518/148‑1 (MQ=255)
            gATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCg                                                                                                                                    <  1:98794/148‑1 (MQ=255)
             atcatcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGc                                                                                                                                   >  1:2331555/1‑148 (MQ=255)
              tcatcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAACGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCa                                                                                                                                  >  2:2512418/1‑148 (MQ=255)
              tcatcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCa                                                                                                                                  >  1:1838786/1‑148 (MQ=255)
               catcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCaa                                                                                                                                 >  2:2402845/1‑148 (MQ=255)
                atcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCaaa                                                                                                                                <  1:2124124/148‑1 (MQ=255)
                 tcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCaaaa                                                                                                                               >  2:214295/1‑148 (MQ=255)
                  cccTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaag                                                                                                                              >  1:2358377/1‑148 (MQ=255)
                   ccTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaaga                                                                                                                             >  1:874218/1‑148 (MQ=255)
                   ccTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaaga                                                                                                                             >  2:63349/1‑148 (MQ=255)
                     tttGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaagaag                                                                                                                           <  2:424620/148‑1 (MQ=255)
                     tttGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaagaag                                                                                                                           >  2:2375127/1‑148 (MQ=255)
                        gATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAgcgc                                                                                                                        >  2:2157586/1‑148 (MQ=255)
                        gATCCCTGCCACGGTGGTGCCGAGATCAACATCGTTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAgcgc                                                                                                                        <  1:941641/148‑1 (MQ=255)
                           cccTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAtt                                                                                                                     >  1:239242/1‑148 (MQ=255)
                             ccgCCCCGCTGGTGCCGAGATCCACAGCGGTAATAATCAACACCGCCCACCAAGACTGATTCCACCCGTAGATAAAGGAGATCCCCCACAGCGCCGCCAGATTAGTTTTGGAGACCGGAAAAACGATGTCGCAAAAGAAGCGCATTgg                                                                                                                   <  2:133475/146‑1 (MQ=255)
                             cTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTgg                                                                                                                   >  2:2974228/1‑148 (MQ=255)
                              tGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGc                                                                                                                  <  2:928824/148‑1 (MQ=255)
                                     gtggtgCCGAGATCCACCTCGGTAATAATCACCAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAACCAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATgcgc                                                                                                           <  2:1499233/148‑1 (MQ=255)
                                     gtggtgCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATgcgc                                                                                                           <  1:3134969/148‑1 (MQ=255)
                                      tggtgCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGcc                                                                                                          >  1:3261107/1‑148 (MQ=255)
                                        gtgCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGt                                                                                                        <  2:1437898/148‑1 (MQ=255)
                                            cGAGATCCACATCGGTAATAATCAACCACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGAt                                                                                                    <  1:2147546/148‑1 (MQ=255)
                                            cGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGAt                                                                                                    <  2:502551/148‑1 (MQ=255)
                                                aTCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgc                                                                                                >  2:1199462/1‑148 (MQ=255)
                                                aTCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgc                                                                                                >  1:2553565/1‑148 (MQ=255)
                                                aTCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgc                                                                                                >  1:2793885/1‑148 (MQ=255)
                                                aTCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgc                                                                                                >  1:2793884/1‑148 (MQ=255)
                                                    acaTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCgg                                                                                            <  2:2968831/148‑1 (MQ=255)
                                                    acaTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCgg                                                                                            >  2:232828/1‑148 (MQ=255)
                                                           taataaTCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTccac                                                                                     >  1:2765481/1‑148 (MQ=255)
                                                                 tCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTc                                                                               <  2:1056060/148‑1 (MQ=255)
                                                                  caacaaCGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCa                                                                              >  1:2362074/1‑148 (MQ=255)
                                                                   aacaacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCAt                                                                             >  1:1483777/1‑148 (MQ=255)
                                                                    acaacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATc                                                                            <  2:2929242/148‑1 (MQ=255)
                                                                     caacGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATcc                                                                           <  2:2543165/148‑1 (MQ=255)
                                                                       acGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCgg                                                                         <  2:1352692/148‑1 (MQ=255)
                                                                       acGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCgg                                                                         <  2:3301406/148‑1 (MQ=255)
                                                                       acGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCgg                                                                         <  2:2075782/148‑1 (MQ=255)
                                                                        cGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGc                                                                        >  2:746562/1‑148 (MQ=255)
                                                                         ggCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCa                                                                       <  1:2634652/148‑1 (MQ=255)
                                                                          gCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAg                                                                      >  2:1496010/1‑148 (MQ=255)
                                                                          gCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAg                                                                      >  2:2205174/1‑148 (MQ=255)
                                                                          gCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAg                                                                      >  2:2790403/1‑148 (MQ=255)
                                                                             ccaAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGt                                                                   <  2:1245396/147‑1 (MQ=255)
                                                                             acaAATACTGAGTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGt                                                                   <  2:2830527/148‑1 (MQ=255)
                                                                              caAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTc                                                                  <  1:3113343/148‑1 (MQ=255)
                                                                               aaaTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGGGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCa                                                                 >  2:1946177/1‑148 (MQ=255)
                                                                                 aTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATa                                                               >  2:1585807/1‑148 (MQ=255)
                                                                                   aCTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaaa                                                             >  1:1625514/1‑148 (MQ=255)
                                                                                   aCTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaaa                                                             >  1:191460/1‑148 (MQ=255)
                                                                                   aCTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaaa                                                             >  1:191467/1‑148 (MQ=255)
                                                                                    cTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAg                                                            <  1:729093/148‑1 (MQ=255)
                                                                                      gATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGaa                                                          <  1:90541/148‑1 (MQ=255)
                                                                                        ttCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACt                                                        >  2:1462228/1‑148 (MQ=255)
                                                                                        ttCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCACCGTCATAAAGAACt                                                        >  1:2787192/1‑148 (MQ=255)
                                                                                             gCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCgg                                                   >  1:2159272/1‑148 (MQ=255)
                                                                                                 tAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACa                                               <  1:1300960/148‑1 (MQ=255)
                                                                                                   gATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAgg                                             >  1:1751490/1‑148 (MQ=255)
                                                                                                    aTAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGTCGATGCGCCGTCGATCCGCGCGGCTTCGACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGa                                            <  1:2978422/148‑1 (MQ=255)
                                                                                                    aTAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGa                                            <  1:1922425/148‑1 (MQ=255)
                                                                                                     tAAAGGTGATCACAAACAGCGCCGCCAGATTATTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGaa                                           <  2:1980203/148‑1 (MQ=255)
                                                                                                     tAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGaa                                           >  1:2098702/1‑148 (MQ=255)
                                                                                                      aaaGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGaaa                                          <  2:2487298/148‑1 (MQ=255)
                                                                                                        aGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGt                                        <  2:2702338/148‑1 (MQ=255)
                                                                                                         ggTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTa                                       <  1:93582/148‑1 (MQ=255)
                                                                                                         ggTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTa                                       >  1:691244/1‑148 (MQ=255)
                                                                                                         ggTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTa                                       >  2:3130522/1‑148 (MQ=255)
                                                                                                          gTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAg                                      >  2:376036/1‑148 (MQ=255)
                                                                                                           tGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGc                                     <  1:517658/148‑1 (MQ=255)
                                                                                                                  aaaCAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGcc                              <  1:1419308/148‑1 (MQ=255)
                                                                                                                    aCAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGa                            <  2:1683482/148‑1 (MQ=255)
                                                                                                                        cgccgcCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGcc                        >  2:3045305/1‑148 (MQ=255)
                                                                                                                          ccgccAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCAt                      <  1:2688735/148‑1 (MQ=255)
                                                                                                                          ccgccAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCAt                      <  2:214067/148‑1 (MQ=255)
                                                                                                                           cgccAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATc                     >  1:517232/1‑148 (MQ=255)
                                                                                                                            gccAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCa                    <  1:371942/148‑1 (MQ=255)
                                                                                                                            gccAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCa                    <  1:2038405/148‑1 (MQ=255)
                                                                                                                             ccAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAg                   >  2:675178/1‑148 (MQ=255)
                                                                                                                                  ttAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCa              >  1:1225043/1‑148 (MQ=255)
                                                                                                                                      ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          >  2:389819/1‑148 (MQ=255)
                                                                                                                                      ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          <  1:479109/148‑1 (MQ=255)
                                                                                                                                      ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          >  2:726149/1‑148 (MQ=255)
                                                                                                                                      ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          <  1:2124865/148‑1 (MQ=255)
                                                                                                                                      ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          <  1:1275361/148‑1 (MQ=255)
                                                                                                                                              agCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCgg  >  2:1119292/1‑148 (MQ=255)
                                                                                                                                              agCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCgg  >  2:1547245/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGG  >  CP009273/3582662‑3582951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑