Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,588,904 A→T 5.2% L287Q (CTG→CAG)  livH ← leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,588,9040AT 5.2% 529.6 / 14.6 173L287Q (CTG→CAG) livHleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (72/92);  new base T (2/7);  total (74/99)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 178 total aligned reads displayed.

AGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATCCC  >  CP009273/3588758‑3589049
                                                                                                                                                  |                                                                                                                                                 
aGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATcagcag                                                                                                                                                  <  2:2027383/148‑1 (MQ=255)
  ttGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTggg                                                                                                                                                <  1:2023923/148‑1 (MQ=255)
  ttGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAggg                                                                                                                                                >  1:2297273/1‑148 (MQ=255)
  ttGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAggg                                                                                                                                                <  1:1668613/148‑1 (MQ=255)
   tGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGc                                                                                                                                               >  1:1071805/1‑148 (MQ=255)
      aCGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGaa                                                                                                                                            <  1:2071337/148‑1 (MQ=255)
        gCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATg                                                                                                                                          <  1:3096340/148‑1 (MQ=255)
         cccATAAAGACGCCCGCCAGCACACAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATga                                                                                                                                         <  2:1719481/148‑1 (MQ=255)
           cATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATgaga                                                                                                                                       <  2:1071805/148‑1 (MQ=255)
              aaaGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTACAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACca                                                                                                                                    <  2:778182/148‑1 (MQ=255)
                aGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACcaca                                                                                                                                  <  1:537972/148‑1 (MQ=255)
                   cgcccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCt                                                                                                                               <  1:3024847/148‑1 (MQ=255)
                   cgcccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCt                                                                                                                               <  1:3059484/148‑1 (MQ=255)
                   cgcccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCt                                                                                                                               <  1:613450/148‑1 (MQ=255)
                   cgcccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCt                                                                                                                               <  1:1378002/148‑1 (MQ=255)
                     cccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCttt                                                                                                                             <  1:1041619/148‑1 (MQ=255)
                     cccgccAGCACAAAGAACAGCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCACACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGACTCAGCAGGGCGAATGAGACCACATCttt                                                                                                                             <  2:1806824/148‑1 (MQ=255)
                      ccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTta                                                                                                                            <  1:1818531/148‑1 (MQ=255)
                      ccgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTta                                                                                                                            <  1:2103064/148‑1 (MQ=255)
                       cgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTtat                                                                                                                           <  2:450211/148‑1 (MQ=255)
                       cgccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTtat                                                                                                                           >  1:2109666/1‑148 (MQ=255)
                         ccAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTatat                                                                                                                         <  2:1450215/148‑1 (MQ=255)
                             cacaAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCg                                                                                                                     >  2:572208/1‑148 (MQ=255)
                              acaAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGt                                                                                                                    <  2:2930085/148‑1 (MQ=255)
                               caAAGAACATCGCGGCAGAGAGCAGCGCTATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTa                                                                                                                   <  2:3297757/148‑1 (MQ=255)
                                aaaGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTAc                                                                                                                  >  1:140849/1‑148 (MQ=255)
                                    aaCATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAg                                                                                                              <  1:893768/148‑1 (MQ=255)
                                     aCATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGa                                                                                                             >  2:1644642/1‑148 (MQ=255)
                                     aCATCGCGGCAGAGAGCAGCGCCAGTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGa                                                                                                             <  2:238020/148‑1 (MQ=255)
                                        tCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAg                                                                                                          >  1:2606013/1‑148 (MQ=255)
                                              cAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCagaa                                                                                                    <  1:2618118/148‑1 (MQ=255)
                                               agagagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCagaag                                                                                                   <  1:950017/148‑1 (MQ=255)
                                               agagagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCagaag                                                                                                   <  2:2863900/148‑1 (MQ=255)
                                                gagagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCagaaga                                                                                                  >  1:915608/1‑148 (MQ=255)
                                                gagagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCagaaga                                                                                                  <  1:1519129/148‑1 (MQ=255)
                                                 agagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAagag                                                                                                 <  2:2367229/148‑1 (MQ=255)
                                                 agagCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAagag                                                                                                 <  1:223978/148‑1 (MQ=255)
                                                   agcagcGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAagagag                                                                                               >  1:400970/1‑148 (MQ=255)
                                                         gcCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTc                                                                                         <  2:508073/148‑1 (MQ=255)
                                                         gcCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTc                                                                                         <  2:2801986/148‑1 (MQ=255)
                                                          ccATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTcc                                                                                        >  2:2189945/1‑148 (MQ=255)
                                                             ttGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCa                                                                                     <  1:1883735/148‑1 (MQ=255)
                                                                cAATATGCATCGGTTTCATACTGTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATc                                                                                  <  2:1888868/148‑1 (MQ=255)
                                                                   tatGCATCGTTTTAATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCTTAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCTTACTCAGATAGGCTGAAGAGAGCGCCTCCGCAATcccc                                                                               <  1:2086178/148‑1 (MQ=255)
                                                                   tatGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATcccc                                                                               <  1:2724038/148‑1 (MQ=255)
                                                                    atGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCa                                                                              >  1:345254/1‑148 (MQ=255)
                                                                       cATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGaa                                                                           <  1:3146920/148‑1 (MQ=255)
                                                                        aTCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAAt                                                                          <  2:2748445/148‑1 (MQ=255)
                                                                         tCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATc                                                                         <  2:3270391/148‑1 (MQ=255)
                                                                         tCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGAATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATc                                                                         <  1:2051680/148‑1 (MQ=255)
                                                                         tCGGTTTCATACTTTCTCGACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATc                                                                         <  2:3270383/148‑1 (MQ=255)
                                                                          cGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCa                                                                        >  2:78179/1‑148 (MQ=255)
                                                                             tttCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGgc                                                                     >  1:2642300/1‑148 (MQ=255)
                                                                                 aTACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGgccgcc                                                                 >  2:2303491/1‑148 (MQ=255)
                                                                                  tACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCa                                                                <  1:2325544/148‑1 (MQ=255)
                                                                                   aCTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCaa                                                               >  1:1333255/1‑148 (MQ=255)
                                                                                    cTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAat                                                              >  2:407114/1‑148 (MQ=255)
                                                                                    cTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAat                                                              <  1:2756556/148‑1 (MQ=255)
                                                                                      ttCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAatca                                                            >  2:2994242/1‑148 (MQ=255)
                                                                                       tctcTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCATAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAatcat                                                           <  1:585870/148‑1 (MQ=255)
                                                                                       tctcTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAatcat                                                           >  2:214189/1‑148 (MQ=255)
                                                                                        ctctACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAatcatc                                                          >  2:1612899/1‑148 (MQ=255)
                                                                                         tctACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCg                                                         >  2:2630449/1‑148 (MQ=255)
                                                                                         tctACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCg                                                         >  2:3194968/1‑148 (MQ=255)
                                                                                            aCCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTc                                                      <  2:896097/148‑1 (MQ=255)
                                                                                              cTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTccc                                                    >  1:1159059/1‑148 (MQ=255)
                                                                                              cTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTccc                                                    >  2:3150035/1‑148 (MQ=255)
                                                                                                  gggCGACCCAGACTACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGaa                                                <  2:1903934/148‑1 (MQ=255)
                                                                                                  gggCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGaa                                                <  2:1903928/148‑1 (MQ=255)
                                                                                                    gCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAAtg                                              <  1:1967437/148‑1 (MQ=255)
                                                                                                     cGACCCCGAATCCCGGGCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAAtgc                                             <  1:2170803/148‑1 (MQ=255)
                                                                                                     cGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAAtgc                                             <  1:1643331/148‑1 (MQ=255)
                                                                                                       aCCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAAtgctg                                           >  2:1171415/1‑148 (MQ=255)
                                                                                                          cAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCa                                        >  2:2397609/1‑148 (MQ=255)
                                                                                                          cAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCa                                        >  2:663889/1‑148 (MQ=255)
                                                                                                          cAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCa                                        >  2:1289267/1‑148 (MQ=255)
                                                                                                            gAATACCGGTCGGCATCCCCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATCTTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAAt                                      <  1:581086/148‑1 (MQ=255)
                                                                                                            gAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAAt                                      <  1:2766010/148‑1 (MQ=255)
                                                                                                            gAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAAt                                      <  2:2667098/148‑1 (MQ=255)
                                                                                                             aaTACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATc                                     >  1:2664968/1‑148 (MQ=255)
                                                                                                               tACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATccc                                   <  2:2300126/148‑1 (MQ=255)
                                                                                                                aCCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATcccc                                  >  2:3083957/1‑147 (MQ=255)
                                                                                                                aCCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCa                                  <  2:947526/148‑1 (MQ=255)
                                                                                                                    gTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGa                              >  1:1868642/1‑148 (MQ=255)
                                                                                                                        gCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCAc                          >  2:2553537/1‑148 (MQ=255)
                                                                                                                        gCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCAc                          >  1:127191/1‑148 (MQ=255)
                                                                                                                        gCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCAc                          >  1:127326/1‑148 (MQ=255)
                                                                                                                               cagcagCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCcgcg                   >  2:112158/1‑148 (MQ=255)
                                                                                                                                agcagcACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCgg                  <  2:2313511/148‑1 (MQ=255)
                                                                                                                                 gcagcaCCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGt                 >  1:1302580/1‑148 (MQ=255)
                                                                                                                                  cagcaCCAGAATCAGCTGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCggaa                >  2:824009/1‑146 (MQ=255)
                                                                                                                                    gcaCCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTaaa              >  2:1735234/1‑148 (MQ=255)
                                                                                                                                    gcaCCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTaaa              <  1:2136431/148‑1 (MQ=255)
                                                                                                                                     caCCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAg             >  1:1979043/1‑148 (MQ=255)
                                                                                                                                     caCCAGAATCAGCAGGGCAAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAg             >  1:1611365/1‑148 (MQ=255)
                                                                                                                                        cAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCt          >  2:2261202/1‑148 (MQ=255)
                                                                                                                                          gAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCttt        >  1:1514607/1‑148 (MQ=255)
                                                                                                                                            aTCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCa      >  1:2784413/1‑148 (MQ=255)
                                                                                                                                              cagcagGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATc    >  2:350980/1‑148 (MQ=255)
                                                                                                                                                gcagGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATccc  >  1:1675237/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGTTGCACGCCCATAAAGACGCCCGCCAGCACAAAGAACATCGCGGCAGAGAGCAGCGCCATTGCAATATGCATCGGTTTCATACTTTCTCTACCTCCGGGCGACCCAGAATACCGGTCGGCATCACCAGCAGCACCAGAATCAGCAGGGCGAATGAGACCACATCTTTATATTCCGTACTCAGATAGGCAGAAGAGAGCGCCTCCGCAATCCCCAGAATCAGGCCGCCAATCATCGCTCCCGGAATGCTGCCAATCCCACCGAGCACCGCCGCGGTAAAGGCTTTCATCCC  >  CP009273/3588758‑3589049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑