Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,604,608 C→A 6.3% A162A (GCG→GCT)  yhhS ← putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,604,6080CA 6.3% 712.7 / 16.8 190A162A (GCG→GCT) yhhSputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (94/84);  new base A (7/5);  total (101/89)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 208 total aligned reads displayed.

CAGCGGTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAGG  >  CP009273/3604461‑3604755
                                                                                                                                                   |                                                                                                                                                   
cAGCGGTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACccatcgccatc                                                                                                                                                     >  1:277131/1‑148 (MQ=255)
    ggTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGccc                                                                                                                                                 <  2:3031151/148‑1 (MQ=255)
        tGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTa                                                                                                                                             <  2:765521/148‑1 (MQ=255)
         gCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTaa                                                                                                                                            <  1:2459458/148‑1 (MQ=255)
         gCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTaa                                                                                                                                            <  2:2267017/148‑1 (MQ=255)
          ccTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAg                                                                                                                                           <  1:1576053/148‑1 (MQ=255)
          ccTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGGAAg                                                                                                                                           >  2:3107020/1‑148 (MQ=255)
           cTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCCTCGCCATCGCCCCGTAAGt                                                                                                                                          <  2:2917753/148‑1 (MQ=255)
            tttACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTg                                                                                                                                         <  2:1279107/148‑1 (MQ=255)
             ttACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGa                                                                                                                                        <  2:829813/148‑1 (MQ=255)
             ttACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGa                                                                                                                                        <  1:2481906/148‑1 (MQ=255)
             ttACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGa                                                                                                                                        <  1:2167301/148‑1 (MQ=255)
               aCTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACa                                                                                                                                      <  2:645461/148‑1 (MQ=255)
                 tGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAAt                                                                                                                                    <  2:14910/148‑1 (MQ=255)
                    cTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGcc                                                                                                                                 >  1:115112/1‑148 (MQ=255)
                     ttttACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCg                                                                                                                                >  2:1884333/1‑148 (MQ=255)
                      tttACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGt                                                                                                                               <  2:2155780/148‑1 (MQ=255)
                       ttACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGtt                                                                                                                              >  2:1115223/1‑148 (MQ=255)
                        tACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTc                                                                                                                             <  1:2088611/148‑1 (MQ=255)
                          ccGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCa                                                                                                                           >  1:1714490/1‑148 (MQ=255)
                                 aCGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATc                                                                                                                    >  2:189476/1‑148 (MQ=255)
                                   gcgGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAc                                                                                                                  <  1:3256752/148‑1 (MQ=255)
                                   gcgGGATCGCCAACAAAAGGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAc                                                                                                                  <  1:1402493/148‑1 (MQ=255)
                                    cgGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcc                                                                                                                 >  2:1569146/1‑148 (MQ=255)
                                       gATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcccgc                                                                                                              >  2:1023578/1‑148 (MQ=255)
                                        aTCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcccgcc                                                                                                             >  2:3044964/1‑148 (MQ=255)
                                             caacaaAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGata                                                                                                        >  1:2957236/1‑148 (MQ=255)
                                                   aaTGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGc                                                                                                  >  2:2671228/1‑148 (MQ=255)
                                                    aTGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCg                                                                                                 >  2:1400155/1‑148 (MQ=255)
                                                    aTGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCg                                                                                                 >  1:2201927/1‑148 (MQ=255)
                                                    aTGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCg                                                                                                 <  1:2663136/148‑1 (MQ=255)
                                                    aTGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCg                                                                                                 <  2:1301307/148‑1 (MQ=255)
                                                    aTGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATAACCCGCCCGATATGCAGCg                                                                                                 >  1:539905/1‑148 (MQ=255)
                                                      ggCCACCAGCGCCACGCCCAAAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAAAGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATTCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAg                                                                                               >  2:440834/1‑148 (MQ=255)
                                                       cccaccaGCGCCACGCCCATAATTATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGc                                                                                              <  1:573890/147‑1 (MQ=255)
                                                         caccaGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCa                                                                                            <  2:2571834/148‑1 (MQ=255)
                                                         caccaGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCa                                                                                            <  1:1357468/148‑1 (MQ=255)
                                                          accaGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCaa                                                                                           <  1:2964333/148‑1 (MQ=255)
                                                          accaGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCaa                                                                                           >  1:3111130/1‑148 (MQ=255)
                                                             aGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCa                                                                                        <  2:2721507/148‑1 (MQ=255)
                                                              gcgcCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCAc                                                                                       >  1:157628/1‑148 (MQ=255)
                                                                  cACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCa                                                                                   >  1:2880404/1‑148 (MQ=255)
                                                                  cACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCa                                                                                   <  1:193352/148‑1 (MQ=255)
                                                                     gCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACg                                                                                <  2:3290699/148‑1 (MQ=255)
                                                                        cATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGccc                                                                             >  1:2848075/1‑148 (MQ=255)
                                                                         aTAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGcccc                                                                            >  2:38419/1‑148 (MQ=255)
                                                                          tAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCa                                                                           >  1:1070248/1‑148 (MQ=255)
                                                                            aTGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATa                                                                         <  2:119146/148‑1 (MQ=255)
                                                                            aTGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATa                                                                         <  2:122572/148‑1 (MQ=255)
                                                                             tGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAg                                                                        >  2:1626225/1‑148 (MQ=255)
                                                                              gATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATGTGCAGCGAGCCAACCACGCCAACGCCCCATAgg                                                                       >  2:2603563/1‑148 (MQ=255)
                                                                               aTTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAggg                                                                      >  2:2278583/1‑148 (MQ=255)
                                                                                       taacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATccc                                                              >  1:87125/1‑148 (MQ=255)
                                                                                        aacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTACCGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCg                                                             <  1:302845/148‑1 (MQ=255)
                                                                                        aacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCg                                                             >  1:440974/1‑148 (MQ=255)
                                                                                        aacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCg                                                             <  1:3168954/148‑1 (MQ=255)
                                                                                        aacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCg                                                             <  1:1530873/148‑1 (MQ=255)
                                                                                          cgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGtt                                                           >  2:2824004/1‑148 (MQ=255)
                                                                                           gcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTc                                                          <  2:1988093/148‑1 (MQ=255)
                                                                                            ccTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTcc                                                         >  1:961668/1‑148 (MQ=255)
                                                                                            ccTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTcc                                                         >  1:1990388/1‑148 (MQ=255)
                                                                                                  aGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCaaa                                                   >  1:2620132/1‑148 (MQ=255)
                                                                                                  aGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCaaa                                                   >  2:3298388/1‑148 (MQ=255)
                                                                                                    ccgccCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAAc                                                 >  1:2450858/1‑148 (MQ=255)
                                                                                                    ccgccCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAAc                                                 >  2:1387531/1‑148 (MQ=255)
                                                                                                     cgccCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACt                                                <  2:572296/148‑1 (MQ=255)
                                                                                                         ccAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGc                                            >  2:256994/1‑148 (MQ=255)
                                                                                                         ccAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGc                                            >  1:23229/1‑148 (MQ=255)
                                                                                                          cAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGcc                                           <  2:2318996/148‑1 (MQ=255)
                                                                                                          cAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGcc                                           >  1:2162274/1‑148 (MQ=255)
                                                                                                          cAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGcc                                           >  1:3082733/1‑148 (MQ=255)
                                                                                                               aTAAATCACGAAGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATc                                      <  1:1581416/148‑1 (MQ=255)
                                                                                                                tAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATcc                                     >  2:791249/1‑148 (MQ=255)
                                                                                                                  aaaCACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCa                                   <  1:606132/148‑1 (MQ=255)
                                                                                                                   aaCACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCaa                                  >  1:1189402/1‑148 (MQ=255)
                                                                                                                    acacGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAg                                 >  1:1619642/1‑148 (MQ=255)
                                                                                                                      acgacgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGa                               <  2:1972634/148‑1 (MQ=255)
                                                                                                                      acgacgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGACCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGa                               >  2:2145074/1‑148 (MQ=255)
                                                                                                                        gacgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATg                             <  1:1234404/148‑1 (MQ=255)
                                                                                                                        gacgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATg                             <  1:397973/148‑1 (MQ=255)
                                                                                                                        gacgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATg                             <  1:1634784/148‑1 (MQ=255)
                                                                                                                         acgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGa                            >  2:434338/1‑148 (MQ=255)
                                                                                                                           gCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGAcg                          <  2:245143/148‑1 (MQ=255)
                                                                                                                              tAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATACCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACgcgc                       >  1:2915756/1‑148 (MQ=255)
                                                                                                                                aCGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGccc                     >  1:707424/1‑148 (MQ=255)
                                                                                                                                aCGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGccc                     >  2:536113/1‑148 (MQ=255)
                                                                                                                                    cgcACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAgg                 >  1:2610788/1‑148 (MQ=255)
                                                                                                                                      cACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCa               >  1:119056/1‑148 (MQ=255)
                                                                                                                                       aCCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCaa              <  2:23229/148‑1 (MQ=255)
                                                                                                                                       aCCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCaa              <  2:44742/148‑1 (MQ=255)
                                                                                                                                           atcgccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGta          <  2:277131/148‑1 (MQ=255)
                                                                                                                                           atcgccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGta          <  1:2147642/148‑1 (MQ=255)
                                                                                                                                           atcgccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGta          <  2:1810263/148‑1 (MQ=255)
                                                                                                                                            tcgccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGtaa         <  1:2699074/148‑1 (MQ=255)
                                                                                                                                             cgccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGtaat        >  1:2833936/1‑148 (MQ=255)
                                                                                                                                             cgccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGtaat        >  1:777245/1‑148 (MQ=255)
                                                                                                                                             cgccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGtaat        >  1:1022805/1‑148 (MQ=255)
                                                                                                                                               ccatcgccCCGTAAGTGACACTGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGtaataa      <  2:2932364/148‑1 (MQ=255)
                                                                                                                                               ccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGtaataa      <  1:1115223/148‑1 (MQ=255)
                                                                                                                                                   cgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAgg  >  2:2084241/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGCGGTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAGG  >  CP009273/3604461‑3604755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑