BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,649,535	G→A	6.2%	Q40* (CAG→TAG)	hdeB ←	acid‑resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,649,535	0	G	A	6.2%	808.4 / 26.7	226	Q40* (CAG→TAG)	hdeB	acid‑resistance protein
Reads supporting (aligned to +/- strand): ref base G (112/100); new base A (7/7); total (119/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 232 total aligned reads displayed.

TCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATAT  >  CP009273/3649388‑3649682

tCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTg                                                                                                                                                     >  1:2019138/1‑148 (MQ=255)

       gTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTc                                                                                                                                              >  1:2772389/1‑148 (MQ=255)

         aTTCGATCACTTTAGGAATTTGATTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCat                                                                                                                                            <  2:2523394/148‑1 (MQ=255)

          ttCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCata                                                                                                                                           <  2:2060666/148‑1 (MQ=255)

              aTCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCtt                                                                                                                                       <  1:2359945/148‑1 (MQ=255)

              aTCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCtt                                                                                                                                       >  2:661081/1‑148 (MQ=255)

               tCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCttt                                                                                                                                      <  1:1926090/148‑1 (MQ=255)

                cACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTa                                                                                                                                     >  2:2013751/1‑148 (MQ=255)

                 aCTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAg                                                                                                                                    >  2:883504/1‑148 (MQ=255)

                   tttAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCg                                                                                                                                  >  2:171186/1‑148 (MQ=255)

                    ttAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCgg                                                                                                                                 >  1:315414/1‑148 (MQ=255)

                    ttAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCgg                                                                                                                                 >  2:619884/1‑148 (MQ=255)

                    ttAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCgg                                                                                                                                 >  2:2421494/1‑148 (MQ=255)

                        gAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTc                                                                                                                             <  1:1696710/148‑1 (MQ=255)

                         aaTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCa                                                                                                                            <  1:1763583/148‑1 (MQ=255)

                         aaTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCa                                                                                                                            <  1:190461/148‑1 (MQ=255)

                          aTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCAt                                                                                                                           <  2:1573392/148‑1 (MQ=255)

                          aTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCAt                                                                                                                           <  2:2841341/148‑1 (MQ=255)

                           tttGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCAtt                                                                                                                          >  2:1983309/1‑148 (MQ=255)

                              gagtgagATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGc                                                                                                                       <  1:543892/148‑1 (MQ=255)

                                   agaTCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCa                                                                                                                  <  2:2210945/148‑1 (MQ=255)

                                       cGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGc                                                                                                              >  1:1796427/1‑148 (MQ=255)

                                          tttCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGa                                                                                                           <  1:3059630/148‑1 (MQ=255)

                                             cATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATtg                                                                                                        >  1:408524/1‑148 (MQ=255)

                                              aTTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATtgt                                                                                                       <  1:989011/148‑1 (MQ=255)

                                                ttAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGc                                                                                                     <  2:1360531/148‑1 (MQ=255)

                                                ttAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGc                                                                                                     >  1:1497056/1‑148 (MQ=255)

                                                      tAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCAc                                                                                               >  1:993602/1‑148 (MQ=255)

                                                      tAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCAc                                                                                               >  1:2974723/1‑148 (MQ=255)

                                                        aCGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAAATCCTGGCAGGTCATATCTTTAGCGGATTCAATGGCTGCCAACGCAGATTGTGCGTTCACCa                                                                                             >  1:3017039/1‑148 (MQ=255)

                                                         cGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTAGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAg                                                                                            >  2:339484/1‑148 (MQ=255)

                                                           gTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTg                                                                                          >  2:2176408/1‑148 (MQ=255)

                                                            tATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGa                                                                                         >  1:1828921/1‑148 (MQ=255)

                                                             aTCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGAc                                                                                        >  1:2732255/1‑148 (MQ=255)

                                                             aTCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGAc                                                                                        >  1:2566539/1‑148 (MQ=255)

                                                               cGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACaa                                                                                      >  2:1141823/1‑148 (MQ=255)

                                                               cGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACaa                                                                                      >  1:874931/1‑148 (MQ=255)

                                                                gCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACaaa                                                                                     <  1:883504/148‑1 (MQ=255)

                                                                 ccACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAg                                                                                    <  1:2094849/148‑1 (MQ=255)

                                                                 ccACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTAGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAg                                                                                    >  1:765078/1‑148 (MQ=255)

                                                                  cACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGc                                                                                   >  2:2602627/1‑148 (MQ=255)

                                                                    ccTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAATGCAGATTGTGCGTTCACCAGTGACAAAGCCg                                                                                 >  1:2856016/1‑148 (MQ=255)

                                                                       ttATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTa                                                                              >  1:2726408/1‑148 (MQ=255)

                                                                       ttATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTa                                                                              >  1:1462646/1‑148 (MQ=255)

                                                                           ataCTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAgc                                                                          >  1:2062773/1‑148 (MQ=255)

                                                                           ataCTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAgc                                                                          >  2:1072110/1‑148 (MQ=255)

                                                                              cTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGcc                                                                       <  2:1466022/148‑1 (MQ=255)

                                                                               tGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGccc                                                                      >  1:2320958/1‑148 (MQ=255)

                                                                               tGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGccc                                                                      >  1:2525973/1‑148 (MQ=255)

                                                                                  ttcttcATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATa                                                                   <  2:2922804/148‑1 (MQ=255)

                                                                                  ttcttcATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCATGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGACGCTACAGCGCCCATa                                                                   >  1:2311111/1‑148 (MQ=255)

                                                                                     ttcATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATaaaa                                                                <  2:829523/148‑1 (MQ=255)

                                                                                      tcATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATaaaaa                                                               >  1:646378/1‑148 (MQ=255)

                                                                                      tcATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATaaaaa                                                               >  2:2081462/1‑148 (MQ=255)

                                                                                       cATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAAt                                                              <  2:510382/148‑1 (MQ=255)

                                                                                         tGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATaa                                                            >  1:2420224/1‑148 (MQ=255)

                                                                                         tGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATaa                                                            >  1:3039638/1‑148 (MQ=255)

                                                                                         tGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATaa                                                            <  2:2566539/148‑1 (MQ=255)

                                                                                          gcagcaTCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATaaa                                                           <  1:842203/148‑1 (MQ=255)

                                                                                          gcagcaTCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATaaa                                                           <  1:1404357/148‑1 (MQ=255)

                                                                                           cagcaTCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAAc                                                          <  1:2651291/148‑1 (MQ=255)

                                                                                            agcaTCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACg                                                         <  2:1712810/148‑1 (MQ=255)

                                                                                            agcaTCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACg                                                         <  1:1390667/148‑1 (MQ=255)

                                                                                             gcaTCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGc                                                        >  1:704837/1‑148 (MQ=255)

                                                                                              caTCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCt                                                       <  2:2355709/148‑1 (MQ=255)

                                                                                                tCCACCATGCAACCGGGGTCATTGCTTTTTGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCttt                                                     <  2:1956711/148‑1 (MQ=255)

                                                                                                tCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCttt                                                     >  1:1004307/1‑148 (MQ=255)

                                                                                                tCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCttt                                                     >  2:736652/1‑148 (MQ=255)

                                                                                                tCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCttt                                                     >  1:2450380/1‑148 (MQ=255)

                                                                                                 ccaccaTGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTa                                                    >  1:1181963/1‑148 (MQ=255)

                                                                                                       tGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGgaga                                              <  1:564460/148‑1 (MQ=255)

                                                                                                            ccGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGa                                         >  2:1595574/1‑148 (MQ=255)

                                                                                                            ccGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGa                                         >  1:163524/1‑148 (MQ=255)

                                                                                                               gggTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAat                                      >  2:581819/1‑148 (MQ=255)

                                                                                                               gggTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAat                                      <  2:2772389/148‑1 (MQ=255)

                                                                                                               gggTCATTGCTTTTGGATTCAGATCAATAAATTCCTAGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAat                                      <  2:1922439/148‑1 (MQ=255)

                                                                                                                ggTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAata                                     >  2:1842160/1‑148 (MQ=255)

                                                                                                                  tCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATAtt                                   >  1:2243061/1‑148 (MQ=255)

                                                                                                                  tCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATAtt                                   >  2:3262732/1‑148 (MQ=255)

                                                                                                                  tCATTGCTTTTGGATTCAGATCAATAAATTCCTAGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATAtt                                   <  2:3048224/148‑1 (MQ=255)

                                                                                                                   cATTGCTTTTGGATTCAGATCAATAAATTCCTAGCAGGTCATATCTTTAGCGGCTTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAATGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTc                                  <  2:1194314/148‑1 (MQ=255)

                                                                                                                      tGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCAtt                               >  1:73203/1‑148 (MQ=255)

                                                                                                                      tGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTAAtt                               <  2:408524/148‑1 (MQ=255)

                                                                                                                         ttttGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTg                            >  1:2490641/1‑148 (MQ=255)

                                                                                                                         ttttGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTg                            <  1:1977756/148‑1 (MQ=255)

                                                                                                                           ttGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTa                          >  2:952931/1‑148 (MQ=255)

                                                                                                                           ttGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTa                          <  2:306676/148‑1 (MQ=255)

                                                                                                                           ttGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTa                          <  2:307075/148‑1 (MQ=255)

                                                                                                                                ttCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCa                     <  2:450119/148‑1 (MQ=255)

                                                                                                                                  cAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCAtt                   <  1:374548/148‑1 (MQ=255)

                                                                                                                                     aTCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCaa                <  1:1003903/148‑1 (MQ=255)

                                                                                                                                        aaTAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAatat             <  2:749096/148‑1 (MQ=255)

                                                                                                                                         aTAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAAtata            <  1:720735/148‑1 (MQ=255)

                                                                                                                                            aaTTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATagag         <  2:1151399/148‑1 (MQ=255)

                                                                                                                                             aTTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAgaga        <  1:2169459/148‑1 (MQ=255)

                                                                                                                                             aTTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAgaga        >  2:2143825/1‑148 (MQ=255)

                                                                                                                                               tCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGAtt      <  2:1301403/148‑1 (MQ=255)

                                                                                                                                                  tGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATtata   <  2:2450380/148‑1 (MQ=255)

                                                                                                                                                   ggCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATtattt  <  1:1298313/148‑3 (MQ=255)

                                                                                                                                                   ggCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATtatat  <  1:571928/148‑1 (MQ=255)

TCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATAT  >  CP009273/3649388‑3649682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`