Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,717,788 C→A 100% P297P (CCG→CCT)  glyQ ← glycine tRNA synthetase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,717,7880CA100.0% 609.0 / NA 182P297P (CCG→CCT) glyQglycine tRNA synthetase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (109/73);  total (109/73)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

AGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTC  >  CP009273/3717644‑3717932
                                                                                                                                                |                                                                                                                                                
aGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAggg                                                                                                                                               <  1:2308027/148‑1 (MQ=255)
       tCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGa                                                                                                                                        >  1:758064/1‑148 (MQ=255)
          aGCTCCGCAGTAAAGTTCGCAGCAAATGACTCAGCCAGGCTGCGCAGTGCTTTTGGAGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCTCCTCTTACTTATCTTTGATGCACATAGGGAAGCCGAggg                                                                                                                                     >  2:2151669/1‑148 (MQ=255)
          aGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGTGAAGCCGAggg                                                                                                                                     >  2:2151632/1‑148 (MQ=255)
           gCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGc                                                                                                                                    >  1:2266078/1‑148 (MQ=255)
           gCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGc                                                                                                                                    <  1:2593926/148‑1 (MQ=255)
           gCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGc                                                                                                                                    <  1:283714/148‑1 (MQ=255)
            cTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCt                                                                                                                                   >  1:1231626/1‑148 (MQ=255)
            cTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCt                                                                                                                                   <  1:651104/148‑1 (MQ=255)
             tCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCtt                                                                                                                                  <  2:234045/148‑1 (MQ=255)
                    tAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGa                                                                                                                           >  2:1942708/1‑148 (MQ=255)
                    tAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGa                                                                                                                           >  2:2311079/1‑148 (MQ=255)
                    tAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGa                                                                                                                           >  1:1910626/1‑148 (MQ=255)
                    tAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGa                                                                                                                           >  1:1303258/1‑148 (MQ=255)
                     aaaGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGaa                                                                                                                          >  2:2868986/1‑148 (MQ=255)
                        gTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCg                                                                                                                       >  1:1042412/1‑148 (MQ=255)
                                  aaGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCtt                                                                                                             <  1:1269713/148‑1 (MQ=255)
                                  aaGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCtt                                                                                                             <  1:2240989/148‑1 (MQ=255)
                                  aaGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCtt                                                                                                             <  2:1008077/148‑1 (MQ=255)
                                  aaGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCtt                                                                                                             >  1:2279833/1‑148 (MQ=255)
                                   aGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTc                                                                                                            >  1:1256396/1‑148 (MQ=255)
                                    ggACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCTGCTATTCAGTGCAGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGTGAAGCCGTGGGATTCACGGGAAGCGTAGTATGCTTCt                                                                                                           <  2:547230/148‑1 (MQ=255)
                                    ggACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCt                                                                                                           <  2:2646361/148‑1 (MQ=255)
                                    ggACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCt                                                                                                           >  2:2835280/1‑148 (MQ=255)
                                      aCTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGc                                                                                                         >  1:463832/1‑148 (MQ=255)
                                        tCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCa                                                                                                       <  1:859860/148‑1 (MQ=255)
                                        tCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCa                                                                                                       >  1:2947288/1‑148 (MQ=255)
                                         cAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCAc                                                                                                      >  1:1132304/1‑148 (MQ=255)
                                         cAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCAc                                                                                                      <  2:2376964/148‑1 (MQ=255)
                                         cAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCAc                                                                                                      <  1:139194/148‑1 (MQ=255)
                                          aGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACt                                                                                                     >  1:432455/1‑148 (MQ=255)
                                           gCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTg                                                                                                    >  2:621739/1‑148 (MQ=255)
                                            ccAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGc                                                                                                   >  1:2124040/1‑148 (MQ=255)
                                             cAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCt                                                                                                  <  2:2223330/148‑1 (MQ=255)
                                                    cgcAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCa                                                                                           <  2:1617955/148‑1 (MQ=255)
                                                    cgcAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCa                                                                                           <  2:1617986/148‑1 (MQ=255)
                                                      cAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAgg                                                                                         <  2:2535392/148‑1 (MQ=255)
                                                           cTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCg                                                                                    >  1:38925/1‑148 (MQ=255)
                                                            ttttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGa                                                                                   >  1:2595784/1‑148 (MQ=255)
                                                            ttttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGa                                                                                   >  2:3200121/1‑148 (MQ=255)
                                                             tttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGaa                                                                                  >  2:2794808/1‑148 (MQ=255)
                                                              ttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAAt                                                                                 <  2:594198/148‑1 (MQ=255)
                                                                     cAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGa                                                                          >  2:2293589/1‑148 (MQ=255)
                                                                     cAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGa                                                                          <  1:178361/148‑1 (MQ=255)
                                                                     cAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGa                                                                          <  2:432455/148‑1 (MQ=255)
                                                                      aGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGaa                                                                         <  1:1520369/148‑1 (MQ=255)
                                                                        ctctTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATg                                                                       <  2:463832/148‑1 (MQ=255)
                                                                        ctctTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATg                                                                       >  1:2544997/1‑148 (MQ=255)
                                                                         tctTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGt                                                                      <  2:3211008/148‑1 (MQ=255)
                                                                           ttCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAg                                                                    >  2:2694899/1‑148 (MQ=255)
                                                                            tCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAgc                                                                   >  2:3133730/1‑148 (MQ=255)
                                                                            tCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAgc                                                                   >  1:109993/1‑148 (MQ=255)
                                                                              aGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAgcgc                                                                 <  1:2442663/148‑1 (MQ=255)
                                                                                tGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTg                                                               >  2:2960968/1‑148 (MQ=255)
                                                                                tGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTg                                                               >  2:2314046/1‑148 (MQ=255)
                                                                                 gCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGa                                                              <  1:943273/148‑1 (MQ=255)
                                                                                    gATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGc                                                           >  2:327940/1‑148 (MQ=255)
                                                                                    gATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGc                                                           >  2:1864479/1‑148 (MQ=255)
                                                                                     aTTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCt                                                          >  2:2612814/1‑148 (MQ=255)
                                                                                     aTTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCt                                                          <  1:3184515/148‑1 (MQ=255)
                                                                                     aTTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCt                                                          <  1:1543188/148‑1 (MQ=255)
                                                                                     aTTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCt                                                          >  1:1208796/1‑148 (MQ=255)
                                                                                            ccaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGAc                                                   >  1:2617742/1‑148 (MQ=255)
                                                                                            ccaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGAc                                                   >  2:499174/1‑148 (MQ=255)
                                                                                              aGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACgg                                                 <  2:1092974/148‑1 (MQ=255)
                                                                                              aGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACgg                                                 >  1:893926/1‑148 (MQ=255)
                                                                                               gAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGga                                                >  2:7992/1‑148 (MQ=255)
                                                                                                aaaaGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGgag                                               <  1:2654830/148‑1 (MQ=255)
                                                                                                aaaaGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGgag                                               <  1:2516084/148‑1 (MQ=255)
                                                                                                 aaaGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGgaga                                              >  2:1162783/1‑148 (MQ=255)
                                                                                                       ttCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCtt                                        <  2:1231626/148‑1 (MQ=255)
                                                                                                        tctcAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCttt                                       <  2:2279833/148‑1 (MQ=255)
                                                                                                           cAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTAcg                                    >  1:169140/1‑148 (MQ=255)
                                                                                                              aCATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACgcgc                                 >  2:2947225/1‑148 (MQ=255)
                                                                                                              aCATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACgcgc                                 >  2:1299509/1‑148 (MQ=255)
                                                                                                               cATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGAGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCa                                >  2:2867801/1‑148 (MQ=255)
                                                                                                                   gccgccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCca                            >  2:173580/1‑148 (MQ=255)
                                                                                                                    ccgccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcag                           >  2:1498231/1‑148 (MQ=255)
                                                                                                                     cgccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagc                          <  2:2091236/148‑1 (MQ=255)
                                                                                                                      gccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagca                         <  1:1610002/148‑1 (MQ=255)
                                                                                                                      gccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagca                         <  1:2158849/148‑1 (MQ=255)
                                                                                                                      gccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagca                         <  1:3246921/148‑1 (MQ=255)
                                                                                                                      gccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagca                         <  1:3246643/148‑1 (MQ=255)
                                                                                                                        ctctTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAgg                       <  2:516724/148‑1 (MQ=255)
                                                                                                                         tctTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGt                      >  1:1686208/1‑148 (MQ=255)
                                                                                                                         tctTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGt                      <  2:1910626/148‑1 (MQ=255)
                                                                                                                         tctTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGt                      >  1:972788/1‑148 (MQ=255)
                                                                                                                          cttacttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGtt                     >  1:2509263/1‑148 (MQ=255)
                                                                                                                             acttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGaa                  >  1:436243/1‑148 (MQ=255)
                                                                                                                             acttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGaa                  >  1:2100505/1‑148 (MQ=255)
                                                                                                                             acttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGaa                  <  1:1320808/148‑1 (MQ=255)
                                                                                                                                taTCTATGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCt               <  2:1398458/148‑1 (MQ=255)
                                                                                                                                 aTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCtg              >  1:2386637/1‑148 (MQ=255)
                                                                                                                                        ttgCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggcgg       <  2:312637/148‑1 (MQ=255)
                                                                                                                                         tgCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggcggc      >  1:653166/1‑148 (MQ=255)
                                                                                                                                         tgCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggcggc      <  2:2617344/148‑1 (MQ=255)
                                                                                                                                         tgCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggcggc      >  1:1339786/1‑148 (MQ=255)
                                                                                                                                           cacaTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCtt    >  1:2679295/1‑148 (MQ=255)
                                                                                                                                            acaTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCttt   <  2:1303258/148‑1 (MQ=255)
                                                                                                                                             caTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTc  >  2:2493754/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTC  >  CP009273/3717644‑3717932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑