Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,791,063 T→C 8.6% A252A (GCT→GCC)  waaL → O‑antigen ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,791,0630TC 8.6% 756.0 / 50.5 244A252A (GCT→GCC) waaLO‑antigen ligase
Reads supporting (aligned to +/- strand):  ref base T (103/120);  new base C (5/16);  total (108/136)
Fisher's exact test for biased strand distribution p-value = 6.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 250 total aligned reads displayed.

CGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAG  >  CP009273/3790918‑3791210
                                                                                                                                                 |                                                                                                                                                   
cgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATtagctag                                                                                                                                                   <  2:754395/148‑1 (MQ=255)
cgGTACTGTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATtagctag                                                                                                                                                   <  2:1070249/148‑1 (MQ=255)
   tACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCAt                                                                                                                                                >  1:1742042/1‑148 (MQ=255)
   tACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCAt                                                                                                                                                >  1:647919/1‑148 (MQ=255)
    aCTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCAtt                                                                                                                                               >  2:830860/1‑148 (MQ=255)
        tATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGtta                                                                                                                                           >  2:547953/1‑148 (MQ=255)
        tATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCCAGCATTGtta                                                                                                                                           <  2:2715874/148‑1 (MQ=255)
           gTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGttatta                                                                                                                                        >  2:1090869/1‑148 (MQ=255)
               tGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATAtt                                                                                                                                    >  2:72817/1‑148 (MQ=255)
               tGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATAtt                                                                                                                                    >  2:169460/1‑148 (MQ=255)
               tGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATAtt                                                                                                                                    >  1:67329/1‑148 (MQ=255)
                ggCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATAttt                                                                                                                                   >  1:3222083/1‑148 (MQ=255)
                 gcgcTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTta                                                                                                                                  <  1:544225/148‑1 (MQ=255)
                 gcgcTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTta                                                                                                                                  <  2:2041993/148‑1 (MQ=255)
                 gcgcTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTta                                                                                                                                  <  2:377511/148‑1 (MQ=255)
                 gcgcTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTta                                                                                                                                  <  2:2381807/148‑1 (MQ=255)
                  cgcTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTTATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTtaa                                                                                                                                 <  1:796010/148‑1 (MQ=255)
                   gcTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTtaat                                                                                                                                <  1:1061092/148‑1 (MQ=255)
                      aaCACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATaaa                                                                                                                             <  1:955031/148‑1 (MQ=255)
                      aaCACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATaaa                                                                                                                             <  1:2443880/148‑1 (MQ=255)
                      aaCACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATaaa                                                                                                                             <  2:550383/148‑1 (MQ=255)
                       acacAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAAc                                                                                                                            <  1:753828/148‑1 (MQ=255)
                         acaAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCa                                                                                                                          <  2:2830265/148‑1 (MQ=255)
                         acaAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCa                                                                                                                          <  2:473895/148‑1 (MQ=255)
                          caAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCaa                                                                                                                         <  2:307315/148‑1 (MQ=255)
                          caAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCaa                                                                                                                         >  1:2627718/1‑148 (MQ=255)
                             aCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATAc                                                                                                                      <  1:2762123/148‑1 (MQ=255)
                             aCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATAc                                                                                                                      <  1:826447/148‑1 (MQ=255)
                             aCCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATAc                                                                                                                      >  1:2173899/1‑148 (MQ=255)
                                  agCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAAt                                                                                                                 >  2:1351268/1‑148 (MQ=255)
                                      aCCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCt                                                                                                             >  1:1095269/1‑148 (MQ=255)
                                       cccTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCta                                                                                                            >  2:2821440/1‑148 (MQ=255)
                                        ccTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCtat                                                                                                           >  2:2631044/1‑148 (MQ=255)
                                          tACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATaa                                                                                                         >  2:683614/1‑148 (MQ=255)
                                          tACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATaa                                                                                                         <  2:789307/148‑1 (MQ=255)
                                             tCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGa                                                                                                      >  1:2857220/1‑148 (MQ=255)
                                               cTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAg                                                                                                    >  1:116116/1‑148 (MQ=255)
                                                     ccTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTaa                                                                                              <  1:2300944/148‑1 (MQ=255)
                                                      cTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTaaa                                                                                             <  1:3184862/148‑1 (MQ=255)
                                                       tataATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAAt                                                                                            >  2:1681572/1‑148 (MQ=255)
                                                        ataATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATg                                                                                           <  2:618221/148‑1 (MQ=255)
                                                          aaTTTGTGTTGCCGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGAc                                                                                         <  1:635728/148‑1 (MQ=255)
                                                           aTTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACt                                                                                        <  2:1389562/148‑1 (MQ=255)
                                                           aTTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACt                                                                                        >  1:202055/1‑148 (MQ=255)
                                                             ttGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTa                                                                                      <  2:1045771/148‑1 (MQ=255)
                                                                 gtTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAAc                                                                                   <  1:1371441/147‑1 (MQ=255)
                                                                  ttGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAg                                                                                 >  1:880877/1‑148 (MQ=255)
                                                                   tgctgcATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGt                                                                                <  1:3174831/148‑1 (MQ=255)
                                                                     ctgcATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTta                                                                              <  1:1992311/148‑1 (MQ=255)
                                                                          ttAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCa                                                                         >  1:2068968/1‑148 (MQ=255)
                                                                           tAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCaa                                                                        >  1:131898/1‑148 (MQ=255)
                                                                              tAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGc                                                                     <  1:1090256/148‑1 (MQ=255)
                                                                              tAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAAAAGTTATACCAATGc                                                                     >  2:1149898/1‑148 (MQ=255)
                                                                                 cTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTaa                                                                  <  2:2397155/148‑1 (MQ=255)
                                                                                  ttattaTAATAAATCACCCAAGAAATTCACGTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAAt                                                                 <  2:1901204/148‑1 (MQ=255)
                                                                                   tattatAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATa                                                                <  2:3222083/148‑1 (MQ=255)
                                                                                     ttatAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAgt                                                              <  1:1640130/148‑1 (MQ=255)
                                                                                       ataataAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAgtgt                                                            <  2:1742042/148‑1 (MQ=255)
                                                                                          ataaATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTAc                                                         >  2:496208/1‑148 (MQ=255)
                                                                                          ataaATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTAc                                                         >  2:2160225/1‑148 (MQ=255)
                                                                                          ataaATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTAc                                                         <  2:1488013/148‑1 (MQ=255)
                                                                                            aaaTCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAAT‑AACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTc                                                      <  1:989419/148‑1 (MQ=255)
                                                                                            aaaTCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACtt                                                       >  1:1059286/1‑148 (MQ=255)
                                                                                             aaTCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTc                                                      >  2:1204267/1‑148 (MQ=255)
                                                                                              aTCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTcc                                                     <  2:1547983/148‑1 (MQ=255)
                                                                                              aTCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTcc                                                     <  2:172527/148‑1 (MQ=255)
                                                                                               tCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTccc                                                    <  2:2584566/148‑1 (MQ=255)
                                                                                                cACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCt                                                   <  1:549512/148‑1 (MQ=255)
                                                                                                     aaGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTg                                              >  1:1486451/1‑148 (MQ=255)
                                                                                                       gAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCa                                            >  1:2207867/1‑148 (MQ=255)
                                                                                                       gAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCa                                            >  1:127033/1‑148 (MQ=255)
                                                                                                       gAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCa                                            <  1:1720592/148‑1 (MQ=255)
                                                                                                       gAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCa                                            >  1:1802802/1‑148 (MQ=255)
                                                                                                       gAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCa                                            >  1:361402/1‑148 (MQ=255)
                                                                                                          aTTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGa                                         >  2:2176261/1‑148 (MQ=255)
                                                                                                          aTTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGa                                         <  1:2486869/148‑1 (MQ=255)
                                                                                                          aTTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCCAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGa                                         <  1:816351/148‑1 (MQ=255)
                                                                                                            tCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACt                                       <  1:1255067/148‑1 (MQ=255)
                                                                                                               cTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGc                                    >  1:3263824/1‑148 (MQ=255)
                                                                                                                   ctctATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATg                                <  2:2975015/148‑1 (MQ=255)
                                                                                                                   ctctATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATg                                >  1:3010945/1‑148 (MQ=255)
                                                                                                                    tctATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGt                               <  2:867966/148‑1 (MQ=255)
                                                                                                                      tATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTAc                             >  1:479220/1‑148 (MQ=255)
                                                                                                                       aTTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACg                            <  2:2150812/148‑1 (MQ=255)
                                                                                                                        ttGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGa                           >  2:1345421/1‑148 (MQ=255)
                                                                                                                        ttGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGa                           >  1:1576237/1‑148 (MQ=255)
                                                                                                                           ttCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAAt                        >  1:166352/1‑148 (MQ=255)
                                                                                                                                ctaATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGtt                   <  2:647919/148‑1 (MQ=255)
                                                                                                                                 taATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGttt                  >  2:1218631/1‑148 (MQ=255)
                                                                                                                                   aTTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTaa                >  1:1662969/1‑148 (MQ=255)
                                                                                                                                    ttGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTaaa               <  2:1714146/148‑1 (MQ=255)
                                                                                                                                     tGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAat              >  1:1999907/1‑148 (MQ=255)
                                                                                                                                     tGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTAATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAat              <  1:259481/148‑1 (MQ=255)
                                                                                                                                       cTATATTAGCTAGCATTGTTATTATATATAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAatat            <  2:1021304/148‑1 (MQ=255)
                                                                                                                                        tataTTAGCTAGCATTTTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAatata           <  2:2242159/148‑1 (MQ=255)
                                                                                                                                         atatTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAatatat          <  1:866782/148‑1 (MQ=255)
                                                                                                                                         atatTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAatatat          >  2:2474751/1‑148 (MQ=255)
                                                                                                                                           atTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTc        <  1:496208/148‑1 (MQ=255)
                                                                                                                                           atTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTc        <  1:2160225/148‑1 (MQ=255)
                                                                                                                                              agctagcATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATa     <  1:1903012/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTATATTAGCTAGCATTGTTATTATATTTAATAAACCAATACAGAATCGCTATAATGAAGCATTAAATGACTTAAACAGTTATACCAATGCTAATAGTGTTACTTCCCTAGGTGCAAGACTGGCAATGTACGAAATTGGTTTAAATATATTCATAAAG  >  CP009273/3790918‑3791210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑