Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,339 A→C 11.2% Q12P (CAA→CCA) ‡ xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3390AC11.2% 329.2 / 3.2 178Q12P (CAA→CCA) ‡xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (77/74);  new base C (20/6);  total (98/80)
Fisher's exact test for biased strand distribution p-value = 1.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCA  >  CP009273/3822192‑3822480
                                                                                                                                                   |                                                                                                                                             
tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCa                                                                                                                                               >  1:157596/1‑148 (MQ=255)
     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcccc                                                                                                                                          >  1:747606/1‑146 (MQ=255)
     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                          >  2:514572/1‑148 (MQ=255)
     aaaaGTGCATATATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCAACGCTCGAGGCAGAAAATGCGCAACCgg                                                                                                                                          >  2:3283357/1‑148 (MQ=255)
       aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGtt                                                                                                                                        >  2:2802535/1‑148 (MQ=255)
       aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgggt                                                                                                                                        >  1:2603894/1‑146 (MQ=255)
        aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTg                                                                                                                                       >  2:1814102/1‑148 (MQ=255)
        aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTg                                                                                                                                       >  1:3118251/1‑148 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAACCGGTTgcg                                                                                                                                     >  1:2207820/1‑148 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTgcg                                                                                                                                     >  1:2100816/1‑148 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                                                                                                     >  2:1807901/1‑148 (MQ=255)
               ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                >  2:185265/1‑148 (MQ=255)
                 aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTc                                                                                                                              >  2:2537603/1‑148 (MQ=255)
                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCCGACTCa                                                                                                                             >  1:2929881/1‑148 (MQ=255)
                   ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCAGACTCaa                                                                                                                            >  1:1866597/1‑148 (MQ=255)
                    tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaa                                                                                                                           <  2:2849830/148‑1 (MQ=255)
                    taaTTTAAATATTTAGATGAGGATGTGGAAAACTTTTGCTTTTCTCTTTCGGTCAGTTAAAATGCCGGCTTTGCTACCACGGTATTGTTTTCGATTTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCaaa                                                                                                                           <  2:3057568/146‑1 (MQ=255)
                     aaTTTAAATTTTTAGATGAGGATGTGGAAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaaa                                                                                                                          <  2:3055050/147‑1 (MQ=255)
                      aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                                                                                                                         >  2:3307873/1‑148 (MQ=255)
                      aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                                                                                                                         >  2:3112417/1‑148 (MQ=255)
                       tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                        >  2:3097206/1‑148 (MQ=255)
                       tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAACa                                                                                                                        >  1:186070/1‑148 (MQ=255)
                          aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCg                                                                                                                     >  2:1281546/1‑148 (MQ=255)
                          aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATCGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCg                                                                                                                     >  2:1281548/1‑148 (MQ=255)
                           aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGa                                                                                                                    >  2:861032/1‑148 (MQ=255)
                              atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACt                                                                                                                 >  2:2191560/1‑148 (MQ=255)
                              atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACt                                                                                                                 >  2:3120840/1‑148 (MQ=255)
                              atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGCTTGCGCAGACTCAAAACACCGAACt                                                                                                                 >  2:1161444/1‑148 (MQ=255)
                               tttAGATGTGGATGTGGCAAACGTTTTCTTTTCTATTTCGGTCAGCTAAAATGCCCGCTTTGCTACCAGGGGTTTTTTTTCTTTGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCAACCGTTTGCGCAGACTCAAAACAGCGAACTg                                                                                                                <  2:1437263/148‑1 (MQ=255)
                               tttAGATGATGATGTGGCAAACGTTTGATTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTGTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTg                                                                                                                <  2:2239961/148‑1 (MQ=255)
                                ttAGTTGAGGTTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCAGGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGa                                                                                                               <  2:615692/148‑1 (MQ=255)
                                ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACACAAAAAAGCGAAATGa                                                                                                               >  1:2242740/1‑148 (MQ=255)
                                 tcgATGAGGATGTGGCAAACGTTTGCTTTACACTTGCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAt                                                                                                              <  2:3072234/146‑1 (MQ=255)
                                 tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAt                                                                                                              <  2:3071874/148‑1 (MQ=255)
                                    aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACTCAAAAAACCCAACAGATTTa                                                                                                           >  1:1218544/1‑148 (MQ=255)
                                    aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCCAACTGAtata                                                                                                           >  2:854073/1‑148 (MQ=255)
                                         gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAAAACCGCACTGAttttaagtc                                                                                                      >  1:1432548/1‑142 (MQ=255)
                                         gATGTGGCAAACGTTTGCTTTTCTCGTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTTTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                      <  2:2451702/148‑1 (MQ=255)
                                         gATGTGGCAAACGTTTGCTGGTCTCTTTCGGTCCGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCACGAGTCAGAAAATCCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                      <  2:2427952/148‑1 (MQ=255)
                                          aTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAAACGGGTGCGCAGACTCAAAAAATCGAACTGtttttaaggct                                                                                                     >  1:645297/1‑141 (MQ=255)
                                             tgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACACAAAACACCGAACAGATTTACCGTCTTGa                                                                                                  >  1:517551/1‑148 (MQ=255)
                                               gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAg                                                                                                <  2:217341/148‑1 (MQ=255)
                                                   aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCAGACTCAAAACACCGAAATGATTTACCGTCTTGAAGATCg                                                                                            >  1:2302659/1‑148 (MQ=255)
                                                       ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATGGTTTTCGATGTCTGGTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACCGCGCCCTGATTTACCGGCTTGATGAGCGGccg                                                                                        >  2:2027745/1‑148 (MQ=255)
                                                          cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGGCTTGAAGATCGTccgccg                                                                                     >  1:134936/1‑148 (MQ=255)
                                                            tttCTCTTGCGGTCAGAAAACAGGCCCGGTTTGCTACCACGGTTTTGTTTTCGTTGTGTGTTTCCACCCTCGTGTCAGAAATTGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                   <  1:2701423/148‑1 (MQ=255)
                                                             ttCTGTTGCGGTCAGATAAACCGCCCGCTTTGATACCCGGGTTTTGTTTTGGTTGTCTGTTTCCACCCTCGTGTCATAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                  <  2:855072/148‑1 (MQ=255)
                                                              tctcTTTCGGTAAGCTAAAATGCCCGCTTTGCTACCTCGGGTTTTTTTTCGTTGTCTTTTTCCACCGTCGTGTCAGAAAATGCTCAAGGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                 <  2:1949747/148‑1 (MQ=255)
                                                                    tCGGTCAGCTAAAAGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaaa                                                                           <  2:1288808/148‑1 (MQ=255)
                                                                     cgctCAGCTAAATGGCCCTCTTTGCTACCAGGGTTTTGTTTTCGTTGTCTGTTTCCACCCTCTAGACACAAAATGCGCACCGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAc                                                                          <  1:171421/145‑1 (MQ=255)
                                                                     cGGTCCGCTAAAAGGCCCGCTTTGCTACCCCGGTTTTGTTTTCGATGTTTGTTTCCACCCTCGAGTAACAAAATGCGCACCCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAc                                                                          <  1:3018504/148‑1 (MQ=255)
                                                                      ggTCAGCTAAAAGGCCCGCTTGGCCACCTCGGGATTGTTTTCGATGTCTTTTACCACCCTCTAGTCATAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcc                                                                         <  1:1917794/148‑1 (MQ=255)
                                                                        tCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCt                                                                       >  2:2948803/1‑148 (MQ=255)
                                                                        tCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCt                                                                       >  2:3014685/1‑148 (MQ=255)
                                                                         cAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGACAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcccgg                                                                      >  2:2901777/1‑146 (MQ=255)
                                                                              aaagggCCGGCTTTGCTACCAGGGTTTTGTTTGCGTTGTCTGTTACCCCCCTCGAGCCATAAATTGCGCCCCGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                 <  1:400407/143‑1 (MQ=255)
                                                                                 aTGCCCGGTTTCCTACCCGGGTTTTGTTTTCGTTGTCTGTTACCACCCTCGAGTCATAAAAGGCCCACCCGGGGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                              <  1:1226587/148‑1 (MQ=255)
                                                                                 aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTgccgc                                                              >  2:985597/1‑148 (MQ=255)
                                                                                  tGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgcc                                                             <  2:2781455/148‑1 (MQ=255)
                                                                                     cggttttGCTACCTGGGGTTTTTTTTCTTTGTCTGTTCCCACCCTCGAGACATAAAATGCGCACCCGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGt                                                          <  1:975669/144‑1 (MQ=255)
                                                                                     ccGCTTGGCTACCTCGGGTTTGTTTTCGTTGTCTGTTTCCACCCGCGAGACAGAAATTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGt                                                          <  1:975656/148‑1 (MQ=255)
                                                                                     ccGATTTGCTACCTGGTGTTTTTTTTGGTTGTCTTTTACCACCCTCTATTCATAAACTGCGCAACGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGt                                                          <  1:3131442/148‑1 (MQ=255)
                                                                                         tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTTCCGCCTGTCAGc                                                      >  1:3239432/1‑148 (MQ=255)
                                                                                          ttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCCGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                     >  2:1421729/1‑148 (MQ=255)
                                                                                            gCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCCCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                   <  2:2764298/148‑1 (MQ=255)
                                                                                             cTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGGTGCGCCGACTCAAAAAAGCGCACAGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACACAGTTTGGCGCCGGTCAGCATca                                                  >  1:2071832/1‑147 (MQ=255)
                                                                                                     ttttttttttGGATGTCTGTTACCACCCACGAGTCACAAAATGCGCACCGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                          <  1:703610/142‑1 (MQ=255)
                                                                                                     ggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                          >  2:1631259/1‑148 (MQ=255)
                                                                                                      gATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCAGAATCAAAAAAGCCAACAGAATTAACGGCTTTTAGATACTCCCCCGCGTCCACAAACCCAGTTTGCCGCCTGTCAGCAACAGCTGGCGAt                                         >  1:949557/1‑148 (MQ=255)
                                                                                                        ttGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                       <  2:873427/148‑1 (MQ=255)
                                                                                                        tcggtttCGAGGTCTGTTTCCCCCCTCGCGTCAGAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                       <  2:2525574/144‑1 (MQ=255)
                                                                                                         tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                      >  2:436076/1‑148 (MQ=255)
                                                                                                           ttttCGTTGTCTGTTACCACCCTCGCCACAGAAAAGGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                    <  1:545364/148‑1 (MQ=255)
                                                                                                           ttttCGATGTGTGTCTCCACCCTCGAGTCACAAACGGCGCCCCGGGGCGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                    <  1:1143399/148‑1 (MQ=255)
                                                                                                           ttctCGATGTCTGTTACCACCCTCGAGTCACACACGGCCCCCCCGGTGCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                    <  1:2795375/145‑1 (MQ=255)
                                                                                                            tttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCGACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGTAGATCGTCCGCCGCTTCCTCAAACCCGGTTTGGCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                   >  2:2114199/1‑148 (MQ=255)
                                                                                                              tCGATGTCTGTTTCCACCCTCGAGTCACAACCTGCGCCCCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                 <  1:824721/148‑1 (MQ=255)
                                                                                                              tCGATGTCTGTTACCACCCCCGAGACACAAAATGCGCACGGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                 <  1:1028230/148‑1 (MQ=255)
                                                                                                                 aTGTCTTTTACCACCCTCGAGTCAGAAACTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                              <  2:1045283/148‑1 (MQ=255)
                                                                                                                 aTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                              <  2:2699287/148‑1 (MQ=255)
                                                                                                                    tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAACAGCGAACAGATTTACAGTCTTTAAGATCGTCCGCCGCTTCCTCAAAAACTGTTTTCCGCCAGTAAGCATCTGCTGGCGATGTTAGTTGCGGTGa                           >  2:1310327/1‑148 (MQ=255)
                                                                                                                     cTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACATCGAAATGATTTAACGTGTTGTATATAGTCCGCCGCGTCCTCAAACCCTGTTTGGCGCCTGTCAGCATCTGCTGGGGATGTTCGTTGGGGTGAt                          >  1:500523/1‑148 (MQ=255)
                                                                                                                          tCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                     <  2:157596/148‑1 (MQ=255)
                                                                                                                           ccACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCACACTCAAAAAACCGAACTGATTTAACGTCTTGAAGATCCTCCGCCGCTTCCTCAAACCCTGGTTGCCGCCAGTCAGCATCTGGTGGCGCTGGTCGTTGCGGTGATCAccgc                    >  2:3044783/1‑145 (MQ=255)
                                                                                                                              cccTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgc                 >  2:286965/1‑148 (MQ=255)
                                                                                                                               ccTCGAGTCAGAAAATGCGCACCCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcg                <  2:1417875/148‑1 (MQ=255)
                                                                                                                                cTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcgc               <  2:2400156/148‑1 (MQ=255)
                                                                                                                                 tCGAGTCAGAAAATGCGCAAACGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTGAAGATAGTCCGCCGCTTCCTCAAAACCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAACACGCCAGCGCt              >  2:1417123/1‑148 (MQ=255)
                                                                                                                                  cGAGCCATAAAAGGCGCACCCGGTGCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa             <  1:2464194/148‑1 (MQ=255)
                                                                                                                                      tCATAAAAGGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTaa         <  2:2603894/148‑1 (MQ=255)
                                                                                                                                      tCACAAAATGCGCCCCGGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTaa         <  1:1052344/148‑1 (MQ=255)
                                                                                                                                       cAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt        <  2:2100669/148‑1 (MQ=255)
                                                                                                                                         gAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt      >  1:2737732/1‑148 (MQ=255)
                                                                                                                                          aataTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg     <  2:934764/145‑1 (MQ=255)
                                                                                                                                          aaaaTGCGCCACCGGTTGCGCAGACTCAAAAAACCGAAATGATTTAACCTCTTTAAGATAGTACGCCGCTTCCACAAAACCAGTTTGCCGCCTGTCAGCAAATGCTGGCGATTTTAGTTGCGGTGATAAACCCAGCGCTATTAATATg     >  2:6856/1‑148 (MQ=255)
                                                                                                                                          aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg     >  2:1364065/1‑148 (MQ=255)
                                                                                                                                           aattGCGCACCGGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc    <  1:2369029/145‑1 (MQ=255)
                                                                                                                                           aaaGGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc    <  1:1001246/148‑1 (MQ=255)
                                                                                                                                             ttGCGCACCGGGTTCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa  <  1:1281546/147‑1 (MQ=255)
                                                                                                                                             aggcgcACCGGGTTCCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa  <  1:45721/146‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCA  >  CP009273/3822192‑3822480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑