Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,861,553 C→A 92.6% G373C (GGC→TGC)  yidR ← DUF3748 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,861,5530CA92.6% 576.5 / 34.7 206G373C (GGC→TGC) yidRDUF3748 family protein
Reads supporting (aligned to +/- strand):  ref base C (9/7);  new base A (95/95);  total (104/102)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

GCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCA  >  CP009273/3861408‑3861697
                                                                                                                                                 |                                                                                                                                                
gCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTcgcagc                                                                                                                                                <  1:491849/148‑1 (MQ=255)
gCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTcgcagc                                                                                                                                                <  1:491760/148‑1 (MQ=255)
 ccTGATAACTGATTTACCGATCAGTTTAGTTGATCCACAGATGGCCGCCTTCCATCCACGCCAGCCACTGACCATACGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCt                                                                                                                                               >  2:2035550/1‑148 (MQ=255)
  cTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCtt                                                                                                                                              >  2:2944309/1‑148 (MQ=255)
   tGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCttt                                                                                                                                             >  2:1390428/1‑148 (MQ=255)
    gATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTg                                                                                                                                            >  2:2209479/1‑148 (MQ=255)
     aTAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTgc                                                                                                                                           >  2:1084754/1‑148 (MQ=255)
         cTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCAt                                                                                                                                       <  2:650947/148‑1 (MQ=255)
         cTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCAt                                                                                                                                       >  1:1967806/1‑148 (MQ=255)
          tGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATg                                                                                                                                      <  1:906363/148‑1 (MQ=255)
             tttAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGc                                                                                                                                   >  1:1088900/1‑148 (MQ=255)
              ttAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGca                                                                                                                                  >  1:3188267/1‑148 (MQ=255)
              ttAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGca                                                                                                                                  >  2:1562902/1‑148 (MQ=255)
                 gCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACaa                                                                                                                               <  1:1817919/148‑1 (MQ=255)
                    aTCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCa                                                                                                                            <  1:1922291/148‑1 (MQ=255)
                    aTCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCa                                                                                                                            <  2:2551724/148‑1 (MQ=255)
                    aTCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCa                                                                                                                            >  2:310660/1‑148 (MQ=255)
                     tCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCGTCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCaa                                                                                                                           <  2:1189477/148‑1 (MQ=255)
                      cAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAt                                                                                                                          <  2:2013054/148‑1 (MQ=255)
                      cAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAt                                                                                                                          >  2:523870/1‑148 (MQ=255)
                       aGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAAtt                                                                                                                         <  2:349492/148‑1 (MQ=255)
                            cGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGAtt                                                                                                                    <  1:592452/148‑1 (MQ=255)
                              gTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTAt                                                                                                                  >  2:1903264/1‑148 (MQ=255)
                              gTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTAt                                                                                                                  <  2:599800/148‑1 (MQ=255)
                               tGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTGGCGCATGGGCACAAGCAATTCGATTATc                                                                                                                 >  2:3028982/1‑148 (MQ=255)
                               tGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTAGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATc                                                                                                                 >  2:2985503/1‑148 (MQ=255)
                                 aTCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCa                                                                                                               >  1:2889865/1‑148 (MQ=255)
                                 aTCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCa                                                                                                               >  1:1953539/1‑148 (MQ=255)
                                   ccACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGc                                                                                                             >  2:128702/1‑148 (MQ=255)
                                      caGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGcaca                                                                                                          >  2:326028/1‑148 (MQ=255)
                                        gCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACaaa                                                                                                        >  1:2908441/1‑148 (MQ=255)
                                         cTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCACTTCGAGTATCCAGCACAAAg                                                                                                       >  1:430372/1‑148 (MQ=255)
                                         cTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAg                                                                                                       <  1:1022591/148‑1 (MQ=255)
                                           ggCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGcc                                                                                                     <  1:2448488/148‑1 (MQ=255)
                                            gccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGccc                                                                                                    >  1:1166653/1‑148 (MQ=255)
                                             ccgccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGTTGAATTGTCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCa                                                                                                   <  2:564467/148‑1 (MQ=255)
                                                ccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAAcc                                                                                                >  1:1471248/1‑148 (MQ=255)
                                                ccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAAcc                                                                                                <  1:2306003/148‑1 (MQ=255)
                                                ccTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAAcc                                                                                                <  1:299794/148‑1 (MQ=255)
                                                 cTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCa                                                                                               <  2:2574739/148‑1 (MQ=255)
                                                    ccatccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATtc                                                                                            <  2:1057896/148‑1 (MQ=255)
                                                     catccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATtct                                                                                           <  2:1919405/148‑1 (MQ=255)
                                                      atccaCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATtctc                                                                                          >  1:2543081/1‑148 (MQ=255)
                                                         caCGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTg                                                                                       >  1:2613764/1‑148 (MQ=255)
                                                              cagccaCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGa                                                                                  <  1:2336710/148‑1 (MQ=255)
                                                                   aCTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCa                                                                             <  1:3310360/148‑1 (MQ=255)
                                                                       aCCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTa                                                                         >  2:1669459/1‑148 (MQ=255)
                                                                        ccATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTaa                                                                        <  1:2525666/148‑1 (MQ=255)
                                                                        ccATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTaa                                                                        <  1:1770289/148‑1 (MQ=255)
                                                                        ccATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTaa                                                                        <  1:100675/148‑1 (MQ=255)
                                                                          aTCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACAGATGCCAGTTAAAt                                                                      <  1:1051795/148‑1 (MQ=255)
                                                                                cGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGAc                                                                <  2:389561/148‑1 (MQ=255)
                                                                                  agaagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTg                                                              >  1:2663047/1‑148 (MQ=255)
                                                                                   gaagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGa                                                             >  2:1967488/1‑148 (MQ=255)
                                                                                   gaagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGa                                                             >  1:3312901/1‑148 (MQ=255)
                                                                                   gaagaCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGa                                                             >  2:2954032/1‑148 (MQ=255)
                                                                                       aCCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAatat                                                         <  1:1151269/148‑1 (MQ=255)
                                                                                         cACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATcc                                                       <  1:311447/148‑1 (MQ=255)
                                                                                         cACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATcc                                                       <  1:310607/148‑1 (MQ=255)
                                                                                           cGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGt                                                     >  2:841148/1‑148 (MQ=255)
                                                                                               gTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTg                                                 <  1:884613/148‑1 (MQ=255)
                                                                                               gTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTg                                                 <  1:1390428/148‑1 (MQ=255)
                                                                                               gTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTg                                                 <  1:2766968/148‑1 (MQ=255)
                                                                                                tCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGt                                                <  2:2908402/148‑1 (MQ=255)
                                                                                                 ccGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGtt                                               >  2:1161256/1‑148 (MQ=255)
                                                                                                 ccGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGtt                                               >  1:2209175/1‑148 (MQ=255)
                                                                                                  cGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTa                                              <  2:1076597/148‑1 (MQ=255)
                                                                                                     aGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATgg                                           <  1:999380/148‑1 (MQ=255)
                                                                                                      gAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATggg                                          <  2:667290/148‑1 (MQ=255)
                                                                                                       aaGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGt                                         >  1:1224748/1‑148 (MQ=255)
                                                                                                         ggTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTa                                       >  1:2348955/1‑148 (MQ=255)
                                                                                                          gTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTaa                                      <  2:1102928/148‑1 (MQ=255)
                                                                                                          gTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTaa                                      <  2:2754845/148‑1 (MQ=255)
                                                                                                               ttGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGgc                                 >  1:3091796/1‑148 (MQ=255)
                                                                                                                tGGCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGgcg                                >  1:444810/1‑148 (MQ=255)
                                                                                                                  gCGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGcgcg                              >  1:3006068/1‑148 (MQ=255)
                                                                                                                   cGTGGTGTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCgg                             >  1:3048295/1‑148 (MQ=255)
                                                                                                                     tggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCt                           >  1:1848156/1‑148 (MQ=255)
                                                                                                                      ggtgTTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTc                          >  1:2187786/1‑148 (MQ=255)
                                                                                                                         gtTCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTcgcc                       >  1:3216774/1‑148 (MQ=255)
                                                                                                                          ttCGGTTAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTcgccg                      >  2:2392059/1‑148 (MQ=255)
                                                                                                                               ttAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtg                 >  1:228616/1‑148 (MQ=255)
                                                                                                                               ttAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtg                 >  1:2932794/1‑148 (MQ=255)
                                                                                                                               ttAAATACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtg                 >  1:1284800/1‑148 (MQ=255)
                                                                                                                                 aaaTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCtgtg               >  1:2276048/1‑148 (MQ=255)
                                                                                                                                  aaTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTgg              <  1:2052860/148‑1 (MQ=255)
                                                                                                                                  aaTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTgg              <  1:2953991/148‑1 (MQ=255)
                                                                                                                                   aTACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGc             >  2:1716697/1‑148 (MQ=255)
                                                                                                                                    tACTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCg            >  1:2972247/1‑148 (MQ=255)
                                                                                                                                      cTCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCgag          <  2:3313809/148‑1 (MQ=255)
                                                                                                                                       tCAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCgaga         <  2:1858262/148‑1 (MQ=255)
                                                                                                                                        cAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGAt        >  2:1496547/1‑148 (MQ=255)
                                                                                                                                        cAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGAt        >  1:704158/1‑148 (MQ=255)
                                                                                                                                        cAACCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGAt        >  1:704119/1‑148 (MQ=255)
                                                                                                                                         ccccTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATa       <  1:2750506/146‑1 (MQ=255)
                                                                                                                                          aCCTCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATaa      >  1:2040555/1‑148 (MQ=255)
                                                                                                                                             tCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGcc   >  1:1526417/1‑148 (MQ=255)
                                                                                                                                             tCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGcc   >  1:1526421/1‑148 (MQ=255)
                                                                                                                                             tCGCAGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGcc   >  2:2501238/1‑148 (MQ=255)
                                                                                                                                              cgcagcTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCca  >  1:3169765/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAATTGGCGTGGTGTTCGGTTAAATACTCAACCTCGCCGCTTTGCGCATGGGCACAAGCAATTCGATTATCCAGCACAAAGCCCAACCATTCTCCTGACGGATGCCAGTTAAATGCAGACTGAATATCCGTTTTGTTATGGGTTAACTGGCGCGGCTCGCCGCCCTGTGGCGAGATAAGCCA  >  CP009273/3861408‑3861697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑