BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,865,863	T→A	85.4%	T166S (ACC→TCC)	dgoD ←	galactonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,865,863	0	T	A	85.4%	520.6 / 69.0	184	T166S (ACC→TCC)	dgoD	galactonate dehydratase
Reads supporting (aligned to +/- strand): ref base T (13/14); new base A (69/88); total (82/102)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

CAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGCCAACCCA  >  CP009273/3865720‑3866004

cAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTa                                                                                                                                           <  1:2942053/148‑1 (MQ=255)

cAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTa                                                                                                                                           >  1:1768253/1‑148 (MQ=255)

cAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTa                                                                                                                                           >  1:1696577/1‑148 (MQ=255)

 aGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTaa                                                                                                                                          <  1:1504451/148‑1 (MQ=255)

 aGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTaa                                                                                                                                          <  1:2288417/148‑1 (MQ=255)

   cACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAcc                                                                                                                                        >  2:1297841/1‑148 (MQ=255)

     ccGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAccgc                                                                                                                                      >  1:61789/1‑148 (MQ=255)

         ctcctcAATAAACTGCGGGCTATACGGCTCCATTTATTTAATCAGCACTTTCGCCATCGGCGCGTTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACTAATTTGTGCCACGGTTTTAACCGCCGCa                                                                                                                                  <  1:2344/148‑1 (MQ=255)

         ctcctcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCCGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCa                                                                                                                                  <  1:3516/148‑1 (MQ=255)

         ctcctcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCa                                                                                                                                  <  2:1095657/148‑1 (MQ=255)

         ctcctcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCa                                                                                                                                  <  1:85486/148‑1 (MQ=255)

         ctcctcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCa                                                                                                                                  >  1:173787/1‑148 (MQ=255)

         ctcctcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACCCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCa                                                                                                                                  >  2:1641939/1‑148 (MQ=255)

                aTAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACcg                                                                                                                           >  2:2050965/1‑148 (MQ=255)

                aTAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACcg                                                                                                                           >  1:1573886/1‑148 (MQ=255)

                       gCGGGCGATACGACTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGa                                                                                                                    <  1:1059046/148‑1 (MQ=255)

                        cGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAg                                                                                                                   <  1:2146332/148‑1 (MQ=255)

                        cGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAg                                                                                                                   >  1:2576326/1‑148 (MQ=255)

                           gCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTa                                                                                                                <  2:3145801/148‑1 (MQ=255)

                           gCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTa                                                                                                                >  1:1016123/1‑148 (MQ=255)

                            cGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTAt                                                                                                               <  1:1267246/148‑1 (MQ=255)

                              aTACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCa                                                                                                             >  2:141218/1‑148 (MQ=255)

                              aTACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCa                                                                                                             <  1:1931174/148‑1 (MQ=255)

                              aTACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATAGCCAAAAGCTTCACGAATTTGTGACACGGTGATAACCGCCGCATCTACCGCGCGGGAGATATCa                                                                                                             >  2:141346/1‑148 (MQ=255)

                               tACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCaa                                                                                                            <  1:2113755/148‑1 (MQ=255)

                                 cGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAAtt                                                                                                          <  2:408880/148‑1 (MQ=255)

                                   gCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAg                                                                                                        >  2:2319966/1‑148 (MQ=255)

                                    cTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGc                                                                                                       >  2:1300816/1‑148 (MQ=255)

                                    cTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGc                                                                                                       >  2:2042156/1‑148 (MQ=255)

                                     tCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGcc                                                                                                      <  1:1662791/148‑1 (MQ=255)

                                      ccAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTCCCGCGCGGGAGTTATCAATTAGccc                                                                                                     >  2:617050/1‑148 (MQ=255)

                                      ccAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGccc                                                                                                     >  2:412687/1‑148 (MQ=255)

                                      ccAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGccc                                                                                                     >  1:2963143/1‑148 (MQ=255)

                                      ccAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGccc                                                                                                     <  2:1895009/148‑1 (MQ=255)

                                         gTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAg                                                                                                  <  2:1179110/148‑1 (MQ=255)

                                           tCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGtt                                                                                                <  2:3065662/148‑1 (MQ=255)

                                           tCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGtt                                                                                                >  2:2364692/1‑148 (MQ=255)

                                           tCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGtt                                                                                                <  2:1455393/148‑1 (MQ=255)

                                            cTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttc                                                                                               <  2:3202940/148‑1 (MQ=255)

                                             tttAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttct                                                                                              <  2:2856060/148‑1 (MQ=255)

                                              ttAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttctt                                                                                             <  1:1568241/148‑1 (MQ=255)

                                              ttAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttctt                                                                                             <  2:1352268/148‑1 (MQ=255)

                                                   cAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACaa                                                                                        <  1:911053/148‑1 (MQ=255)

                                                     gCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCTCAGTTCTTCACAAcc                                                                                      <  2:2223683/148‑1 (MQ=255)

                                                        cTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGtt                                                                                   <  1:2882394/148‑1 (MQ=255)

                                                         tttCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTc                                                                                  >  1:2283448/1‑148 (MQ=255)

                                                         tttCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTc                                                                                  <  1:738713/148‑1 (MQ=255)

                                                         tttCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTc                                                                                  <  2:154325/148‑1 (MQ=255)

                                                          ttCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCa                                                                                 >  1:2270752/1‑148 (MQ=255)

                                                            cGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGt                                                                               <  1:3280090/148‑1 (MQ=255)

                                                             gCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGtt                                                                              <  1:2318352/148‑1 (MQ=255)

                                                                 tCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGaa                                                                          >  1:1521154/1‑148 (MQ=255)

                                                                   ggCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAgg                                                                        <  1:1645958/148‑1 (MQ=255)

                                                                      gcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTAt                                                                     <  2:173787/148‑1 (MQ=255)

                                                                       cgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATc                                                                    <  2:3136229/148‑1 (MQ=255)

                                                                           gACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAg                                                                <  2:1013113/148‑1 (MQ=255)

                                                                           gACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAg                                                                <  2:1013188/148‑1 (MQ=255)

                                                                           gACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAg                                                                <  2:2666801/148‑1 (MQ=255)

                                                                           gACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAg                                                                <  2:2963143/148‑1 (MQ=255)

                                                                             cgcgACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGcc                                                              <  2:629372/148‑1 (MQ=255)

                                                                               cgACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGa                                                            >  2:2885737/1‑148 (MQ=255)

                                                                                gACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGAt                                                           <  1:412687/148‑1 (MQ=255)

                                                                                 aCCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGAtt                                                          <  2:247887/148‑1 (MQ=255)

                                                                                      ggAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTcgcg                                                     >  1:643448/1‑148 (MQ=255)

                                                                                          aTCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGc                                                 >  2:263478/1‑148 (MQ=255)

                                                                                          aTCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGc                                                 >  1:1381310/1‑148 (MQ=255)

                                                                                             aaGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGtt                                              <  1:1427019/148‑1 (MQ=255)

                                                                                             aaGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGtt                                              <  1:3105010/148‑1 (MQ=255)

                                                                                             aaGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGtt                                              >  2:2662392/1‑148 (MQ=255)

                                                                                             aaGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGtt                                              <  1:3105013/148‑1 (MQ=255)

                                                                                              aGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGttt                                             <  1:263681/148‑1 (MQ=255)

                                                                                              aGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGttt                                             <  1:263478/148‑1 (MQ=255)

                                                                                              aGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGttt                                             >  1:515803/1‑148 (MQ=255)

                                                                                               gACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGtttt                                            >  1:2358114/1‑148 (MQ=255)

                                                                                                aCCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTa                                           <  2:477190/148‑1 (MQ=255)

                                                                                                 ccAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTaa                                          >  1:1870820/1‑148 (MQ=255)

                                                                                                  cAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAAt                                         >  1:1251556/1‑148 (MQ=255)

                                                                                                    aaCTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGc                                       >  1:2247652/1‑148 (MQ=255)

                                                                                                     aCTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGcc                                      >  1:3123009/1‑148 (MQ=255)

                                                                                                     aCTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGcc                                      >  2:2128459/1‑148 (MQ=255)

                                                                                                       tCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGt                                    <  2:1855879/148‑1 (MQ=255)

                                                                                                       tCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGt                                    >  2:2286560/1‑148 (MQ=255)

                                                                                                        cAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTc                                   >  1:2699113/1‑148 (MQ=255)

                                                                                                            cTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATa                               >  2:813745/1‑148 (MQ=255)

                                                                                                             tGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATaa                              <  2:2572237/148‑1 (MQ=255)

                                                                                                             tGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATaa                              <  2:2358114/148‑1 (MQ=255)

                                                                                                              gATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAAc                             <  1:2542886/148‑1 (MQ=255)

                                                                                                                ttGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACAt                           >  2:2628625/1‑148 (MQ=255)

                                                                                                                 tGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATc                          >  1:2575569/1‑148 (MQ=255)

                                                                                                                      aaaGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATccgccg                     >  1:1415633/1‑148 (MQ=255)

                                                                                                                       aaGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCgg                    >  1:2334187/1‑148 (MQ=255)

                                                                                                                       aaGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCgg                    >  1:1079743/1‑148 (MQ=255)

                                                                                                                           ttCACGTATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGa                <  1:141346/148‑1 (MQ=255)

                                                                                                                           ttCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGa                <  2:232741/148‑1 (MQ=255)

                                                                                                                           ttCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGa                <  1:141218/148‑1 (MQ=255)

                                                                                                                           ttCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCTCCGGACGa                <  2:293942/148‑1 (MQ=255)

                                                                                                                           ttCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGa                <  1:1720070/148‑1 (MQ=255)

                                                                                                                             cACGAATTTGTGCCACGGAGTTAACCGCCGCATCTCCCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATc              >  1:1612782/1‑148 (MQ=255)

                                                                                                                              aCGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATcg             <  1:181776/148‑1 (MQ=255)

                                                                                                                                 aaTTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATcgccg          >  2:2195702/1‑148 (MQ=255)

CAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGCCAACCCA  >  CP009273/3865720‑3866004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`