Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,887,058 A→T 8.8% K312* (AAA→TAA)  yidZ → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,887,0580AT 8.8% 578.1 / 39.1 193K312* (AAA→TAA) yidZputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (105/71);  new base T (9/8);  total (114/79)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 197 total aligned reads displayed.

ACAATCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGTAAATGATTACTCT  >  CP009273/3886912‑3887204
                                                                                                                                                  |                                                                                                                                                  
 cAATCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTaaa                                                                                                                                                  >  2:937562/1‑148 (MQ=255)
 cAATCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTaaa                                                                                                                                                  <  1:1498000/148‑1 (MQ=255)
    tCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAAc                                                                                                                                               >  2:2252890/1‑148 (MQ=255)
       aCTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCtt                                                                                                                                            >  1:247049/1‑148 (MQ=255)
        cTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCttt                                                                                                                                           <  1:3272570/148‑1 (MQ=255)
         tCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTa                                                                                                                                          >  2:3009937/1‑148 (MQ=255)
         tCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACATTTa                                                                                                                                          >  2:2883030/1‑148 (MQ=255)
           cATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTAcg                                                                                                                                        >  1:3210581/1‑148 (MQ=255)
              cAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGt                                                                                                                                     >  2:2695543/1‑148 (MQ=255)
              cAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGt                                                                                                                                     >  2:1820873/1‑148 (MQ=255)
                 cTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGa                                                                                                                                  >  2:182167/1‑148 (MQ=255)
                   gCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATg                                                                                                                                >  2:743991/1‑148 (MQ=255)
                       ttggttgCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCAt                                                                                                                            >  1:905660/1‑148 (MQ=255)
                        tggttgCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATa                                                                                                                           >  1:2774898/1‑148 (MQ=255)
                         ggttgCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATaa                                                                                                                          <  2:1482337/148‑1 (MQ=255)
                            tgCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCg                                                                                                                       >  2:2251876/1‑148 (MQ=255)
                              ctctTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTTAAAACCTTTACGCGTCGATGGCATAACCGaa                                                                                                                     <  1:1573735/148‑1 (MQ=255)
                              ctctTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTTAAAACCTTTACGCGTCGATGGCATAACCGaa                                                                                                                     <  1:1572905/148‑1 (MQ=255)
                                  tCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGt                                                                                                                 <  2:2734517/148‑1 (MQ=255)
                                   ccTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTa                                                                                                                >  1:538555/1‑148 (MQ=255)
                                    ctctCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTAt                                                                                                               >  1:240919/1‑148 (MQ=255)
                                     tctcCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATg                                                                                                              >  2:2980714/1‑148 (MQ=255)
                                     tctcCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATg                                                                                                              >  2:3227348/1‑148 (MQ=255)
                                       tcCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGaa                                                                                                            <  1:3070993/148‑1 (MQ=255)
                                       tcCCGATTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGaa                                                                                                            <  1:415382/148‑1 (MQ=255)
                                          cGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAAcg                                                                                                         <  2:1260140/148‑1 (MQ=255)
                                          cGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAAcg                                                                                                         <  2:2909539/148‑1 (MQ=255)
                                           gTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAAcgc                                                                                                        >  1:235835/1‑148 (MQ=255)
                                            tttGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAAcgcg                                                                                                       <  2:966365/148‑1 (MQ=255)
                                            tttGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAAcgcg                                                                                                       >  1:26653/1‑148 (MQ=255)
                                             ttGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGa                                                                                                      >  2:1597174/1‑148 (MQ=255)
                                               gACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGAcc                                                                                                    <  2:2322899/148‑1 (MQ=255)
                                               gACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGAcc                                                                                                    <  2:3040437/148‑1 (MQ=255)
                                                   aaaGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAAt                                                                                                <  1:2252890/148‑1 (MQ=255)
                                                   aaaGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAAt                                                                                                <  2:3152772/148‑1 (MQ=255)
                                                   aaaGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAAt                                                                                                >  2:2604804/1‑148 (MQ=255)
                                                     aGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAAttt                                                                                              <  2:1986524/148‑1 (MQ=255)
                                                      gCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTc                                                                                             >  2:152301/1‑148 (MQ=255)
                                                      gCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTc                                                                                             >  1:1214079/1‑148 (MQ=255)
                                                           cAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAAt                                                                                        >  2:254377/1‑148 (MQ=255)
                                                             aaaaaaGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTTAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATaa                                                                                      >  2:2139174/1‑148 (MQ=255)
                                                             aaaaaaGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAAGaa                                                                                      >  2:423146/1‑148 (MQ=255)
                                                              aaaaaGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATaaa                                                                                     <  2:1153247/148‑1 (MQ=255)
                                                              aaaaaGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATaaa                                                                                     <  2:63789/148‑1 (MQ=255)
                                                                aaaGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAAt                                                                                   <  1:2470913/148‑1 (MQ=255)
                                                                 aaGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATg                                                                                  >  1:1154631/1‑148 (MQ=255)
                                                                 aaGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATg                                                                                  >  2:1522607/1‑148 (MQ=255)
                                                                   gCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTa                                                                                >  2:1929907/1‑148 (MQ=255)
                                                                     tGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTaaa                                                                              <  2:256076/148‑1 (MQ=255)
                                                                       gAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTaaaaa                                                                            >  1:399536/1‑148 (MQ=255)
                                                                       gAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTaaaaa                                                                            >  2:2340558/1‑148 (MQ=255)
                                                                        aaGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTaaaaaa                                                                           <  1:3259044/148‑1 (MQ=255)
                                                                         aGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTTAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAg                                                                          <  1:1128810/148‑1 (MQ=255)
                                                                         aGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAg                                                                          <  1:1823622/148‑1 (MQ=255)
                                                                          gTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGt                                                                         >  2:1072620/1‑148 (MQ=255)
                                                                          gTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGt                                                                         >  1:2295040/1‑148 (MQ=255)
                                                                               ttttACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGtaa                                                                    <  1:2732288/148‑1 (MQ=255)
                                                                               ttttACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGtaa                                                                    <  2:253193/148‑1 (MQ=255)
                                                                                tttACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGtaat                                                                   <  2:1744330/148‑1 (MQ=255)
                                                                                  tACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGtaataa                                                                 <  1:1046848/148‑1 (MQ=255)
                                                                                    cccTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGc                                                               <  1:1940059/148‑1 (MQ=255)
                                                                                       tgctgTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTg                                                            <  1:3169380/148‑1 (MQ=255)
                                                                                       tgctgTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTg                                                            <  1:161144/148‑1 (MQ=255)
                                                                                           gtgGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTg                                                        >  1:619165/1‑148 (MQ=255)
                                                                                             ggCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGaa                                                      <  2:1780854/148‑1 (MQ=255)
                                                                                              gCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAAt                                                     <  2:2343345/148‑1 (MQ=255)
                                                                                               cATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATc                                                    >  2:104061/1‑148 (MQ=255)
                                                                                               cATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATc                                                    >  2:2993298/1‑148 (MQ=255)
                                                                                                   aaCGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAAc                                                >  1:449744/1‑148 (MQ=255)
                                                                                                     cGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACtt                                              >  1:49434/1‑148 (MQ=255)
                                                                                                       gAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCTAACtttt                                            >  2:1908945/1‑148 (MQ=255)
                                                                                                       gAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACtttt                                            >  2:672063/1‑148 (MQ=255)
                                                                                                       gAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACtttt                                            <  2:1137776/148‑1 (MQ=255)
                                                                                                        aaCAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTa                                           <  1:730878/148‑1 (MQ=255)
                                                                                                         aCAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTTAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAg                                          <  1:2467628/148‑1 (MQ=255)
                                                                                                           aGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGcc                                        >  1:83064/1‑148 (MQ=255)
                                                                                                            gCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCg                                       >  2:2664881/1‑148 (MQ=255)
                                                                                                             ccATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGc                                      <  2:905660/148‑1 (MQ=255)
                                                                                                                 aaTCCGAAGATCGTCTGGTTACGGGAAACCATTTAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTa                                  >  1:2165580/1‑148 (MQ=255)
                                                                                                                 aaTCCGAAGATCGTCTGGTTACGGGAAACCATTTAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTa                                  >  2:1634477/1‑148 (MQ=255)
                                                                                                                 aaTCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTa                                  >  2:822005/1‑148 (MQ=255)
                                                                                                                   tCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGt                                >  1:1767185/1‑148 (MQ=255)
                                                                                                                      gAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCTTATGAAACGCGACCCAATTTCACAATAATATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTATAGCAGCTTTAGTATg                             >  2:2477765/1‑148 (MQ=255)
                                                                                                                       aaGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGt                            >  2:278315/1‑148 (MQ=255)
                                                                                                                         gATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTcc                          >  2:2726847/1‑148 (MQ=255)
                                                                                                                            cGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCcatc                       <  1:1597174/148‑1 (MQ=255)
                                                                                                                               cTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCAtt                    <  2:240919/148‑1 (MQ=255)
                                                                                                                                  gTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCa                 >  1:457730/1‑148 (MQ=255)
                                                                                                                                  gTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCa                 >  1:1358989/1‑148 (MQ=255)
                                                                                                                                    tACGGGAAACCATTTAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGt               >  1:2051665/1‑148 (MQ=255)
                                                                                                                                        ggAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGTAAAt           <  2:144327/148‑1 (MQ=255)
                                                                                                                                        ggAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCCTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGTAAAt           >  1:3141987/1‑148 (MQ=255)
                                                                                                                                         gAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGTAAATg          >  2:1662138/1‑148 (MQ=255)
                                                                                                                                          aaaCCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGTAAATGa         <  1:2102960/148‑1 (MQ=255)
                                                                                                                                            aCCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGTAAATGAtt       >  1:1016413/1‑148 (MQ=255)
                                                                                                                                            aCCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCAATCATTCCAGTAAATGAtt       >  2:3198135/1‑148 (MQ=255)
                                                                                                                                             ccATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGTAAATGATTa      >  1:2811897/1‑148 (MQ=255)
                                                                                                                                                ttAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGTAAATGATTActc   <  2:325694/148‑1 (MQ=255)
                                                                                                                                                ttAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGTAAATGATTActc   >  1:895389/1‑148 (MQ=255)
                                                                                                                                                 tAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGTAAATGATTActct  <  1:254447/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACAATCAACTCCATCAACTGCCGTTGGTTGCTCTTCCTCTCCCGTTTGACGAAAGCCAGCAAAAAAAGCTGGAAGTTCCTTTTACCCTGCTGTGGCATAAACGGAACAGCCATAATCCGAAGATCGTCTGGTTACGGGAAACCATTAAAAACCTTTACGCGTCGATGGCATAACCGAATCGTATGAAACGCGACCCAATTTCACAATAAAATGTAAAAAAGTTGTAATAAGCTTGTCTGAATCGAACTTTTAGCCGCTTTAGTCTGTCCATCATTCCAGTAAATGATTACTCT  >  CP009273/3886912‑3887204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑