Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,892,304 C→A 87.4% P150P (CCG→CCT)  yieK ← putative 6‑phosphogluconolactonase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,892,3040CA87.4% 453.5 / 66.9 191P150P (CCG→CCT) yieKputative 6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand):  ref base C (15/9);  new base A (104/63);  total (119/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 193 total aligned reads displayed.

CGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAATAGT  >  CP009273/3892157‑3892450
                                                                                                                                                   |                                                                                                                                                  
cgcTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATc                                                                                                                                                    <  2:2101812/148‑1 (MQ=255)
cgcTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATc                                                                                                                                                    <  2:305524/148‑1 (MQ=255)
  cTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAgg                                                                                                                                                  <  1:2586696/148‑1 (MQ=255)
   tttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAggg                                                                                                                                                 >  1:1119083/1‑148 (MQ=255)
    ttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGa                                                                                                                                                >  2:3246267/1‑148 (MQ=255)
     tACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCACCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGaa                                                                                                                                               <  2:1338876/148‑1 (MQ=255)
       cccGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACt                                                                                                                                             <  2:3249448/148‑1 (MQ=255)
         cgcgCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTcc                                                                                                                                           <  1:3080784/148‑1 (MQ=255)
            gccgcTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACg                                                                                                                                        <  1:1577790/148‑1 (MQ=255)
                  gACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGc                                                                                                                                  >  2:2216034/1‑148 (MQ=255)
                  gACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGc                                                                                                                                  >  2:3284953/1‑148 (MQ=255)
                   aCAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCt                                                                                                                                 >  2:2388668/1‑148 (MQ=255)
                    cAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTc                                                                                                                                <  1:2601686/148‑1 (MQ=255)
                    cAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTc                                                                                                                                <  1:748312/148‑1 (MQ=255)
                      aTTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCAt                                                                                                                              <  2:481639/148‑1 (MQ=255)
                       ttATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATg                                                                                                                             >  1:1330810/1‑148 (MQ=255)
                        tATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATgg                                                                                                                            >  1:1133699/1‑148 (MQ=255)
                          tCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGaa                                                                                                                          <  2:3140733/148‑1 (MQ=255)
                          tCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGaa                                                                                                                          >  2:560591/1‑148 (MQ=255)
                          tCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGaa                                                                                                                          >  2:1552554/1‑148 (MQ=255)
                           cAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGaaa                                                                                                                         >  1:1831890/1‑148 (MQ=255)
                           cAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGaaa                                                                                                                         <  2:1918375/148‑1 (MQ=255)
                           cAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGaaa                                                                                                                         >  1:3207542/1‑148 (MQ=255)
                            aGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAAt                                                                                                                        <  2:576948/148‑1 (MQ=255)
                              cAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGaa                                                                                                                          <  1:1729739/144‑1 (MQ=255)
                               aaGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATggg                                                                                                                     <  1:1281890/148‑1 (MQ=255)
                                aGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGt                                                                                                                    <  2:994109/148‑1 (MQ=255)
                                  tttttCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCg                                                                                                                  <  1:654077/148‑1 (MQ=255)
                                   ttttCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGt                                                                                                                 >  2:927350/1‑148 (MQ=255)
                                     ttCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTAt                                                                                                               <  1:236264/148‑1 (MQ=255)
                                      tCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTAtt                                                                                                              <  2:2974707/148‑1 (MQ=255)
                                            ccATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCac                                                                                                        >  2:1845035/1‑147 (MQ=255)
                                            ccATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCaa                                                                                                        >  2:1553779/1‑148 (MQ=255)
                                               tgatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAAtt                                                                                                     >  1:151007/1‑148 (MQ=255)
                                                gatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTg                                                                                                    >  1:3187112/1‑148 (MQ=255)
                                                gatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTg                                                                                                    >  1:3187124/1‑148 (MQ=255)
                                                gatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTg                                                                                                    >  1:473106/1‑148 (MQ=255)
                                                  tgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCACATTGcc                                                                                                  >  2:1256376/1‑148 (MQ=255)
                                                  tgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGcc                                                                                                  <  2:411994/148‑1 (MQ=255)
                                                  tgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGcc                                                                                                  <  1:510465/148‑1 (MQ=255)
                                                  tgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGcc                                                                                                  >  1:128788/1‑148 (MQ=255)
                                                     ttttCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCcaca                                                                                               >  1:349433/1‑148 (MQ=255)
                                                              ccATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGAcc                                                                                      >  2:2710244/1‑148 (MQ=255)
                                                               cATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCa                                                                                     >  1:1696038/1‑148 (MQ=255)
                                                               cATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCa                                                                                     <  2:638122/148‑1 (MQ=255)
                                                                aTCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCCCAAAAATGACCAt                                                                                    >  2:1599441/1‑148 (MQ=255)
                                                                aTCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCAt                                                                                    >  2:508279/1‑148 (MQ=255)
                                                                aTCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCAt                                                                                    >  2:690127/1‑148 (MQ=255)
                                                                aTCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCAt                                                                                    >  2:1635451/1‑148 (MQ=255)
                                                                   gTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTg                                                                                 <  1:608517/148‑1 (MQ=255)
                                                                    tCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGc                                                                                >  1:165401/1‑148 (MQ=255)
                                                                     cACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCa                                                                               >  2:1219578/1‑148 (MQ=255)
                                                                      aCGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCAc                                                                              >  1:1883175/1‑148 (MQ=255)
                                                                      aCGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCAc                                                                              >  2:144393/1‑148 (MQ=255)
                                                                           gCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAAt                                                                         >  1:729010/1‑148 (MQ=255)
                                                                            cTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATc                                                                        <  2:1335411/148‑1 (MQ=255)
                                                                             tGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATcc                                                                       <  2:716155/148‑1 (MQ=255)
                                                                                cAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAg                                                                    <  2:303477/148‑1 (MQ=255)
                                                                                cAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAg                                                                    <  2:141556/148‑1 (MQ=255)
                                                                                     aCTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGcacca                                                               >  1:1103441/1‑148 (MQ=255)
                                                                                       tAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCaccacc                                                             >  2:1166873/1‑148 (MQ=255)
                                                                                        aaCGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCAccacca                                                            >  2:2362699/1‑148 (MQ=255)
                                                                                        aaCGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCAccacca                                                            >  1:8500/1‑148 (MQ=255)
                                                                                        aaCGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCAccacca                                                            >  2:2914578/1‑148 (MQ=255)
                                                                                        aaCGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCAccacca                                                            >  1:11395/1‑148 (MQ=255)
                                                                                          cGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCaaa                                                          <  2:1302542/148‑1 (MQ=255)
                                                                                           gAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAAt                                                         <  2:2226256/148‑1 (MQ=255)
                                                                                             aaaGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATcc                                                       <  1:927350/148‑1 (MQ=255)
                                                                                              aaGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCa                                                      >  1:2657495/1‑148 (MQ=255)
                                                                                                 tCGCCGCCCAACTAGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTc                                                   >  2:1421886/1‑148 (MQ=255)
                                                                                                        ccAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTc                                            <  2:2118718/148‑1 (MQ=255)
                                                                                                              cGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACgcgc                                      <  2:2688918/148‑1 (MQ=255)
                                                                                                              cGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACgcgc                                      <  1:1552560/148‑1 (MQ=255)
                                                                                                              cGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACgcgc                                      <  1:560591/148‑1 (MQ=255)
                                                                                                              cGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACgcgc                                      >  2:2537303/1‑148 (MQ=255)
                                                                                                              cGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGAACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACgcgc                                      >  2:492505/1‑148 (MQ=255)
                                                                                                               gCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGcc                                     >  1:996627/1‑148 (MQ=255)
                                                                                                                 cATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCGAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAg                                   <  1:1801115/148‑1 (MQ=255)
                                                                                                                   tGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGtt                                 >  1:417311/1‑148 (MQ=255)
                                                                                                                       ccACACTATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAAGTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTg                             <  2:1294140/148‑1 (MQ=255)
                                                                                                                       ccACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTg                             <  1:2907735/148‑1 (MQ=255)
                                                                                                                        cacaATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGa                            >  1:1586787/1‑148 (MQ=255)
                                                                                                                        cacaATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGa                            <  1:1763363/148‑1 (MQ=255)
                                                                                                                           aaTATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCa                         <  2:778350/148‑1 (MQ=255)
                                                                                                                            atatCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCAt                        <  1:3246267/148‑1 (MQ=255)
                                                                                                                            atatCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCAt                        >  1:3065347/1‑148 (MQ=255)
                                                                                                                            atatCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCAt                        >  1:801548/1‑148 (MQ=255)
                                                                                                                              atCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGc                      >  1:2155429/1‑148 (MQ=255)
                                                                                                                              atCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGc                      >  1:226171/1‑148 (MQ=255)
                                                                                                                              atCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGc                      >  2:1070232/1‑148 (MQ=255)
                                                                                                                              atCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGc                      >  2:1026233/1‑148 (MQ=255)
                                                                                                                                    cATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCgg                <  2:2680132/148‑1 (MQ=255)
                                                                                                                                       ctctcCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAg             >  2:2817474/1‑148 (MQ=255)
                                                                                                                                         ctcCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGtt           <  2:2017811/148‑1 (MQ=255)
                                                                                                                                         ctcCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGtt           <  2:1674516/148‑1 (MQ=255)
                                                                                                                                          tcCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTa          <  2:1201671/148‑1 (MQ=255)
                                                                                                                                            ccTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATc        >  1:2631597/1‑148 (MQ=255)
                                                                                                                                             cTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCa       >  1:746783/1‑148 (MQ=255)
                                                                                                                                               ggATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAAt     >  1:3307078/1‑148 (MQ=255)
                                                                                                                                                gATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAATa    <  1:629906/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAATAGT  >  CP009273/3892157‑3892450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑