Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,892,978 G→T 6.4% N320K (AAC→AAA)  yieL ← putative xylanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,892,9780GT 6.4% 880.0 / 22.1 234N320K (AAC→AAA) yieLputative xylanase
Reads supporting (aligned to +/- strand):  ref base G (115/104);  new base T (5/10);  total (120/114)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 237 total aligned reads displayed.

CAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGC  >  CP009273/3892831‑3893123
                                                                                                                                                   |                                                                                                                                                 
  gACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCttt                                                                                                                                                 >  2:2910801/1‑148 (MQ=255)
  gACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGtt                                                                                                                                                 <  1:1701132/148‑1 (MQ=255)
  gACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGtt                                                                                                                                                 <  2:760903/148‑1 (MQ=255)
      cGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGt                                                                                                                                             <  2:1558843/148‑1 (MQ=255)
       ggATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTc                                                                                                                                            <  1:491790/148‑1 (MQ=255)
          tatTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTGAGCCCGGCGAAATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCggg                                                                                                                                         >  1:3249843/1‑148 (MQ=255)
           atTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGc                                                                                                                                        <  1:2683727/148‑1 (MQ=255)
             tCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcg                                                                                                                                      >  2:684437/1‑148 (MQ=255)
                  gTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCTTTCAGTCGGGCCGCGaca                                                                                                                                 >  1:2247285/1‑148 (MQ=255)
                   tAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCTTTCAGTCGGGCCGCGacac                                                                                                                                <  2:2802585/148‑1 (MQ=255)
                    aaTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCTTTCAGTCGGGCCGCGACAcc                                                                                                                               <  1:399914/148‑1 (MQ=255)
                    aaTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACAcc                                                                                                                               >  1:1651661/1‑148 (MQ=255)
                    aaTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACAcc                                                                                                                               >  2:2397442/1‑148 (MQ=255)
                    aaTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACAcc                                                                                                                               >  1:520173/1‑148 (MQ=255)
                     aTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCt                                                                                                                              <  2:48537/148‑1 (MQ=255)
                       cAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTc                                                                                                                            >  1:1799421/1‑148 (MQ=255)
                         aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCAt                                                                                                                          <  1:343253/148‑1 (MQ=255)
                         aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCAt                                                                                                                          >  2:1874809/1‑148 (MQ=255)
                          aGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATc                                                                                                                         >  2:331800/1‑148 (MQ=255)
                          aGTTAATTTTTTTCTGCTAAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATc                                                                                                                         <  1:88959/148‑1 (MQ=255)
                           gTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATcc                                                                                                                        >  2:2637725/1‑148 (MQ=255)
                           gTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATcc                                                                                                                        >  2:2637682/1‑148 (MQ=255)
                           gTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATcc                                                                                                                        >  1:3153199/1‑148 (MQ=255)
                              aaTTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGt                                                                                                                     <  1:2718154/148‑1 (MQ=255)
                               aTTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTa                                                                                                                    >  2:570410/1‑148 (MQ=255)
                                tttttttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTAc                                                                                                                   >  1:446661/1‑148 (MQ=255)
                                 ttttttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACg                                                                                                                  <  1:2038938/148‑1 (MQ=255)
                                    tttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGtt                                                                                                               <  1:290636/148‑1 (MQ=255)
                                        tGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCg                                                                                                           <  1:2397442/148‑1 (MQ=255)
                                        tGCTCAAGCTCAGGTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCg                                                                                                           <  1:1951099/148‑1 (MQ=255)
                                           tCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTgg                                                                                                        <  2:1165000/148‑1 (MQ=255)
                                            cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGt                                                                                                       <  2:2150378/148‑1 (MQ=255)
                                            cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGt                                                                                                       >  2:2875170/1‑148 (MQ=255)
                                              aGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTaa                                                                                                     >  2:85717/1‑148 (MQ=255)
                                               gCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAac                                                                                                    >  1:2273634/1‑148 (MQ=255)
                                                cTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAaca                                                                                                   <  2:2963133/148‑1 (MQ=255)
                                                 tCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAacac                                                                                                  >  2:733157/1‑148 (MQ=255)
                                                 tCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAacac                                                                                                  >  2:2389905/1‑148 (MQ=255)
                                                    gTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAAcaccac                                                                                               <  1:3284594/148‑1 (MQ=255)
                                                             ccGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTgg                                                                                      <  1:846580/148‑1 (MQ=255)
                                                             ccGGCGATATCCTTGCCGGGTACGACATCTTTATCCCCCTCAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTgg                                                                                      <  2:1813986/148‑1 (MQ=255)
                                                              cGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGgc                                                                                     <  1:815277/148‑1 (MQ=255)
                                                               ggCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGgcc                                                                                    <  1:1630521/148‑1 (MQ=255)
                                                               ggCGATATCCTTGCCGGTTACGACATCATTATCCCCCACAACCACAGTAAAATGACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGgcc                                                                                    <  2:2019066/148‑1 (MQ=255)
                                                                gCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGgcca                                                                                   <  2:2012468/148‑1 (MQ=255)
                                                                  gATATCCGTGCCGGTTACGACATCTTTATCCCCCACCCCCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGgccagc                                                                                 <  2:3240263/148‑1 (MQ=255)
                                                                   atatCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGgccagcc                                                                                >  2:2229155/1‑148 (MQ=255)
                                                                   atatCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGgccagcc                                                                                >  2:1997932/1‑148 (MQ=255)
                                                                    tatCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGgccagcca                                                                               >  1:2024435/1‑148 (MQ=255)
                                                                     atCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGgccagccag                                                                              >  2:1128337/1‑148 (MQ=255)
                                                                     atCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGgccagccag                                                                              >  1:2092917/1‑148 (MQ=255)
                                                                      tcattGACGGTTACGACATCTTTATCCCCAACAACCACAGTAAAAATACGTAGTTGATGGTTGATAGCTGACGGATCGTTCAGTCGTGCCGCGACACCATCATCCGGTACGGTTGTCGTGGTAACACCACTGAAAGTGgccagccagc                                                                             >  2:1726288/4‑148 (MQ=255)
                                                                         ttGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCaa                                                                          <  1:1862307/148‑1 (MQ=255)
                                                                          tGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCaaa                                                                         >  1:98361/1‑148 (MQ=255)
                                                                          tGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCaaa                                                                         >  1:3115064/1‑148 (MQ=255)
                                                                           gCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCTTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAg                                                                        <  2:542831/148‑1 (MQ=255)
                                                                            ccGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGc                                                                       <  2:1136158/148‑1 (MQ=255)
                                                                                    gACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAga                                                               <  2:3060114/148‑1 (MQ=255)
                                                                                    gACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAga                                                               <  2:8869/148‑1 (MQ=255)
                                                                                    gACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAga                                                               <  2:1415729/148‑1 (MQ=255)
                                                                                    gACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAga                                                               <  1:570410/148‑1 (MQ=255)
                                                                                      cATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAgatg                                                             >  1:2534551/1‑148 (MQ=255)
                                                                                       aTCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAgatga                                                            <  2:305024/148‑1 (MQ=255)
                                                                                        tCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAgatgat                                                           <  1:2844504/148‑1 (MQ=255)
                                                                                        tCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAgatgat                                                           <  1:2845123/148‑1 (MQ=255)
                                                                                         cTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGatt                                                          <  2:665638/148‑1 (MQ=255)
                                                                                         cTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGatt                                                          >  1:1492783/1‑148 (MQ=255)
                                                                                           ttATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGattca                                                        <  1:102573/148‑1 (MQ=255)
                                                                                           ttATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGattca                                                        <  1:1538986/148‑1 (MQ=255)
                                                                                           ttATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGattca                                                        >  2:1366893/1‑148 (MQ=255)
                                                                                              tCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGattcattc                                                     >  2:3104459/1‑148 (MQ=255)
                                                                                              tCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGattcattc                                                     >  1:1152674/1‑148 (MQ=255)
                                                                                              tCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGattcattc                                                     <  2:1991796/148‑1 (MQ=255)
                                                                                                   cacaACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGaa                                                >  2:628385/1‑148 (MQ=255)
                                                                                                   cacaACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGaa                                                >  2:629274/1‑148 (MQ=255)
                                                                                                     caACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAAc                                              <  2:2733046/148‑1 (MQ=255)
                                                                                                        ccACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAg                                           >  1:1905314/1‑148 (MQ=255)
                                                                                                        ccACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAg                                           >  1:2563241/1‑148 (MQ=255)
                                                                                                        ccACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAg                                           <  1:452663/148‑1 (MQ=255)
                                                                                                           caGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAgcgc                                        <  2:3053772/148‑1 (MQ=255)
                                                                                                           caGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAgcgc                                        >  1:2286337/1‑148 (MQ=255)
                                                                                                           caGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAgcgc                                        >  2:315034/1‑148 (MQ=255)
                                                                                                           caGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAgcgc                                        >  2:3206022/1‑148 (MQ=255)
                                                                                                            aGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGcc                                       <  2:2828164/148‑1 (MQ=255)
                                                                                                              tAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTg                                     >  1:2451970/1‑148 (MQ=255)
                                                                                                              tAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTg                                     >  1:253303/1‑148 (MQ=255)
                                                                                                               aaaaTTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTTAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTgg                                    >  2:2302502/1‑148 (MQ=255)
                                                                                                                  aTTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTAc                                 >  2:2587927/1‑148 (MQ=255)
                                                                                                                   ttACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTAcc                                >  2:806235/1‑148 (MQ=255)
                                                                                                                       gTAGTTGCTGGTTGATAGCTGCCGGATCTTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGcc                            <  1:1447522/148‑1 (MQ=255)
                                                                                                                       gTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGcc                            <  1:1997932/148‑1 (MQ=255)
                                                                                                                       gTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGcc                            <  1:1125671/148‑1 (MQ=255)
                                                                                                                          gTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTtg                         <  1:1366893/148‑1 (MQ=255)
                                                                                                                                  ttGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGcc                 >  2:1450031/1‑148 (MQ=255)
                                                                                                                                  ttGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGcc                 <  1:2389905/148‑1 (MQ=255)
                                                                                                                                  ttGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGcc                 <  1:733157/148‑1 (MQ=255)
                                                                                                                                   tGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGccc                >  2:745345/1‑148 (MQ=255)
                                                                                                                                              ggATCTTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAgcgcgc     >  2:1569668/1‑148 (MQ=255)
                                                                                                                                                aTCTTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCgg   <  1:2170737/148‑1 (MQ=255)
                                                                                                                                                aTCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCgg   >  2:2103372/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CAGACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTAACACCACTGAATGTGGCCAGCCAGCCAAAGCTTTCCAGATGATTCATTCCGGAAACCAGCGCCTGGTACCCGCCTTGTGAAAGCCCTGCCAGCGCGCGGC  >  CP009273/3892831‑3893123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑