Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,916,830 C→A 100% D71Y (GAT→TAT)  rsmG ← 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,916,8300CA100.0% 575.9 / NA 171D71Y (GAT→TAT) rsmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (89/82);  total (89/82)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 171 total aligned reads displayed.

GGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTG  >  CP009273/3916685‑3916974
                                                                                                                                                 |                                                                                                                                                
 gTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGAt                                                                                                                                               >  2:279329/1‑148 (MQ=255)
  ttCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATa                                                                                                                                              <  2:1152466/148‑1 (MQ=255)
   tCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGTCTATCCAACATAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATaa                                                                                                                                             <  2:1726377/148‑1 (MQ=255)
   tCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATaa                                                                                                                                             <  2:1415055/148‑1 (MQ=255)
      atatTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAAcc                                                                                                                                          >  1:614501/1‑148 (MQ=255)
      atatTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAAcc                                                                                                                                          >  1:614473/1‑148 (MQ=255)
        atTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGt                                                                                                                                        >  2:3310849/1‑148 (MQ=255)
          tctcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTc                                                                                                                                      <  2:2255680/148‑1 (MQ=255)
          tctcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTc                                                                                                                                      >  2:2140930/1‑148 (MQ=255)
          tctcCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTc                                                                                                                                      >  2:373493/1‑148 (MQ=255)
             ccAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCAcc                                                                                                                                   >  1:118516/1‑148 (MQ=255)
             ccAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCAcc                                                                                                                                   <  1:341624/148‑1 (MQ=255)
               aGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCtt                                                                                                                                 >  2:291250/1‑148 (MQ=255)
                gTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTg                                                                                                                                <  2:282127/148‑1 (MQ=255)
                  ttAAGCTCATGTTGCACCTGACTAAGGAAACGCACGCGTTTACCAAGGCTATCCAACTGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCa                                                                                                                              <  1:8308/148‑1 (MQ=255)
                      gCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATa                                                                                                                          <  1:3208698/148‑1 (MQ=255)
                        tCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACg                                                                                                                        >  2:1315443/1‑148 (MQ=255)
                        tCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACg                                                                                                                        >  2:505623/1‑148 (MQ=255)
                         cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                       <  1:1423241/148‑1 (MQ=255)
                         cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                       <  2:2124139/148‑1 (MQ=255)
                         cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                       >  1:2414575/1‑148 (MQ=255)
                         cATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACgg                                                                                                                       <  1:2507219/148‑1 (MQ=255)
                           tGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTg                                                                                                                     <  2:2612730/148‑1 (MQ=255)
                              tGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGcca                                                                                                                  >  1:526246/1‑148 (MQ=255)
                                  ccTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCcaccac                                                                                                              >  2:2027456/1‑148 (MQ=255)
                                  ccTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCcaccac                                                                                                              >  1:2383671/1‑148 (MQ=255)
                                   cTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACcaca                                                                                                             <  1:2714140/148‑1 (MQ=255)
                                    tGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACaa                                                                                                            <  2:62977/148‑1 (MQ=255)
                                     gACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAAt                                                                                                           >  2:2490991/1‑148 (MQ=255)
                                      aCGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATg                                                                                                          <  2:1083747/148‑1 (MQ=255)
                                       cGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGc                                                                                                         <  1:599956/148‑1 (MQ=255)
                                          aGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTAt                                                                                                      >  1:2594760/1‑148 (MQ=255)
                                            gAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCg                                                                                                    >  2:2644286/1‑148 (MQ=255)
                                             aaaCGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCga                                                                                                   >  2:1543642/1‑148 (MQ=255)
                                              aaCGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCgag                                                                                                  <  1:2766783/148‑1 (MQ=255)
                                               acgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCgaga                                                                                                 <  2:1239739/148‑1 (MQ=255)
                                               acgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCgaga                                                                                                 >  2:2241841/1‑148 (MQ=255)
                                               acgcacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCgaga                                                                                                 >  1:1521601/1‑148 (MQ=255)
                                                  cacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAata                                                                                              <  1:1249281/148‑1 (MQ=255)
                                                  cacgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAata                                                                                              >  1:1431537/1‑148 (MQ=255)
                                                   acgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAatat                                                                                             <  2:365643/148‑1 (MQ=255)
                                                   acgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAatat                                                                                             <  1:559646/148‑1 (MQ=255)
                                                   acgcGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAatat                                                                                             <  1:1479696/148‑1 (MQ=255)
                                                    cgcgTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATg                                                                                            >  2:2728416/1‑148 (MQ=255)
                                                        tttACCAAGGCTATCCAACGGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGt                                                                                        >  2:2731302/1‑148 (MQ=255)
                                                         ttACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTa                                                                                       >  1:2584088/1‑148 (MQ=255)
                                                         ttACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTa                                                                                       >  1:2269093/1‑148 (MQ=255)
                                                         ttACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTa                                                                                       >  2:3239284/1‑148 (MQ=255)
                                                            ccAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCa                                                                                    >  2:429999/1‑148 (MQ=255)
                                                            ccAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCa                                                                                    >  2:1256906/1‑148 (MQ=255)
                                                            ccAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCa                                                                                    >  2:1256802/1‑148 (MQ=255)
                                                              aaGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGc                                                                                  >  2:1675717/1‑148 (MQ=255)
                                                                ggCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAt                                                                                >  1:1093029/1‑148 (MQ=255)
                                                                 gCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCAtc                                                                               >  1:1762580/1‑148 (MQ=255)
                                                                    aTCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCa                                                                            >  1:1707937/1‑148 (MQ=255)
                                                                     tCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCAt                                                                           >  1:2784789/1‑148 (MQ=255)
                                                                     tCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCAt                                                                           >  1:2897791/1‑148 (MQ=255)
                                                                       cAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTa                                                                         >  2:536792/1‑148 (MQ=255)
                                                                         aCAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAgg                                                                       <  1:1068447/148‑1 (MQ=255)
                                                                         aCAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAgg                                                                       >  2:1573131/1‑148 (MQ=255)
                                                                             agTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcg                                                                   <  1:406861/148‑1 (MQ=255)
                                                                              gTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcgc                                                                  <  2:8633/148‑1 (MQ=255)
                                                                               tGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATcgcg                                                                 >  1:1934545/1‑148 (MQ=255)
                                                                                 aaaTGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGa                                                               <  2:1122804/148‑1 (MQ=255)
                                                                                     gggCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGa                                                           >  1:638364/1‑148 (MQ=255)
                                                                                      ggCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGaa                                                          >  2:1950541/1‑148 (MQ=255)
                                                                                       gCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAg                                                         >  1:958206/1‑148 (MQ=255)
                                                                                        cTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGt                                                        >  2:2054422/1‑148 (MQ=255)
                                                                                         ttCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTc                                                       <  2:3027849/148‑1 (MQ=255)
                                                                                              gACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGtt                                                  >  1:1158471/1‑148 (MQ=255)
                                                                                                  cACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTAc                                              >  1:2521843/1‑148 (MQ=255)
                                                                                                   aCGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACg                                             <  1:1783061/148‑1 (MQ=255)
                                                                                                     gATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCt                                           >  1:759522/1‑148 (MQ=255)
                                                                                                     gATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCt                                           <  1:1181336/148‑1 (MQ=255)
                                                                                                      aTAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCtt                                          <  1:996673/148‑1 (MQ=255)
                                                                                                      aTAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCtt                                          <  1:996451/148‑1 (MQ=255)
                                                                                                       tAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCttt                                         >  2:1775731/1‑148 (MQ=255)
                                                                                                       tAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCttt                                         >  2:1572582/1‑148 (MQ=255)
                                                                                                         gagagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGt                                       >  1:184038/1‑148 (MQ=255)
                                                                                                          agagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGtt                                      <  1:443379/148‑1 (MQ=255)
                                                                                                          agagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGtt                                      >  1:2369340/1‑148 (MQ=255)
                                                                                                           gagTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTc                                     <  1:1615906/148‑1 (MQ=255)
                                                                                                            agTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTcc                                    >  2:1901179/1‑148 (MQ=255)
                                                                                                                 aaTGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTa                               <  2:1887010/148‑1 (MQ=255)
                                                                                                                  aTGCCTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTAt                              >  1:216941/1‑148 (MQ=255)
                                                                                                                     ccTGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgca                           <  1:2731302/148‑1 (MQ=255)
                                                                                                                       tGGCAGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATgcagc                         <  2:2278625/148‑1 (MQ=255)
                                                                                                                           aGTCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCatat                     <  2:3002578/148‑1 (MQ=255)
                                                                                                                             tCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTc                   <  1:2086607/148‑1 (MQ=255)
                                                                                                                             tCCTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTc                   >  2:1116016/1‑148 (MQ=255)
                                                                                                                              ccTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCa                  <  1:3076815/148‑1 (MQ=255)
                                                                                                                               cTGGTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCAc                 >  2:3030749/1‑148 (MQ=255)
                                                                                                                                 ggTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGt               <  2:3019041/148‑1 (MQ=255)
                                                                                                                                  gTCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTa              <  1:291250/148‑1 (MQ=255)
                                                                                                                                   tCCGGTGCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAg             >  2:1117558/1‑148 (MQ=255)
                                                                                                                                         gCCGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATa       >  1:2180277/1‑148 (MQ=255)
                                                                                                                                          ccGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATaa      >  1:774876/1‑148 (MQ=255)
                                                                                                                                           cGACATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAg     <  2:2269093/148‑1 (MQ=255)
                                                                                                                                             aCATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCt   >  2:2681414/1‑148 (MQ=255)
                                                                                                                                             aCATAGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCt   <  2:1288603/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTTCAATATTCTCCAGTTTAAGCTCATGTTGCACCTGACGAAGGAAACGCACGCGTTTACCAAGGCTATCCAACAGAGTGAAATGGGCTTCAGGACGCACGATAGAGAGTGGAATGCCTGGCAGTCCTGGTCCGGTGCCGACATCGATAAACCGTTCACCTTGCAGATACGGTGCCACCACAATGCTATCGAGAATATGGCGTACCAGCATCTCATTAGGATCGCGGACCGAAGTCAGGTTGTACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTG  >  CP009273/3916685‑3916974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑