BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,936,831	C→A	90.8%	noncoding (37/76 nt)	gltU →	tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,936,831	0	C	A	90.8%	223.1 / 28.1	130	noncoding (37/76 nt)	gltU	tRNA‑Glu
Reads supporting (aligned to +/- strand): ref base C (6/6); new base A (74/44); total (80/50)
Fisher's exact test for biased strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 130 total aligned reads displayed.

AACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTT  >  CP009273/3936685‑3936903

aaCCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCa                                                                         <  1:858018/148‑1 (MQ=32)

    tGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAAcggc                                                                     >  1:1531977/1‑148 (MQ=17)

     gCGTTTGGATCACCTCCTTACCTTAAAGAAGCTTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTTAAAAGCAAGGCGTCTTGCGAAGCAGAGTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAAcggcg                                                                    <  1:876583/148‑1 (MQ=11)

      cGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAAcggcgg                                                                   >  2:2804999/1‑148 (MQ=17)

       ggTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGt                                                                  <  2:120460/148‑1 (MQ=32)

       ggTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGt                                                                  <  2:120684/148‑1 (MQ=32)

          tGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAAc                                                               >  2:1220275/1‑148 (MQ=17)

          tGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAAc                                                               >  2:1220714/1‑148 (MQ=17)

          tGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAAc                                                               <  2:1589691/148‑1 (MQ=17)

           ggATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACa                                                              >  2:1802289/1‑148 (MQ=17)

            gATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAg                                                             >  1:701736/1‑148 (MQ=17)

             aTCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAgg                                                            <  1:2995970/148‑1 (MQ=17)

             aTCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAgg                                                            <  1:2995713/148‑1 (MQ=17)

             aTCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAgg                                                            <  1:2546523/148‑1 (MQ=17)

               cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAgggg                                                          >  1:1025109/1‑148 (MQ=17)

                cccgccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGt                                                         >  1:3169257/5‑148 (MQ=17)

                aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGt                                                         >  1:3171963/1‑148 (MQ=17)

                aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGt                                                         >  1:3309519/1‑148 (MQ=17)

                 cctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGtt                                                        <  1:2587850/148‑1 (MQ=17)

                 cctcatTACCTTAAAGTAGCGTTCTTTGCAGTTCTCACACAGTTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGAATACTGATACGTCCCGTTCTTCTAGTGGCCAAGGACACCGCCCTTTAACGGCGGTAACAGGGGtt                                                        <  1:2587853/148‑1 (MQ=11)

                  ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTc                                                       >  1:2747266/1‑148 (MQ=17)

                    ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGa                                                     <  1:1699396/148‑1 (MQ=17)

                     cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGaa                                                    <  2:2497288/148‑1 (MQ=17)

                     cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGaa                                                    <  2:150918/148‑1 (MQ=17)

                      ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAAt                                                   >  2:3000195/1‑148 (MQ=17)

                      ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAAt                                                   <  2:1421970/148‑1 (MQ=17)

                      ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAAt                                                   >  1:2910392/1‑148 (MQ=17)

                       taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAATGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATc                                                  >  1:2114702/1‑148 (MQ=17)

                       taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATc                                                  >  1:451312/1‑148 (MQ=17)

                         ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATccc                                                >  2:2632600/1‑148 (MQ=17)

                         ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATccc                                                <  1:2190949/148‑1 (MQ=17)

                         ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGAGTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATccc                                                <  1:114380/148‑1 (MQ=17)

                          cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATcccc                                               >  2:1693129/1‑148 (MQ=17)

                          cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATcccc                                               <  1:2799243/148‑1 (MQ=17)

                          cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATcccc                                               >  2:1692783/1‑148 (MQ=17)

                           ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCt                                              >  1:3241868/1‑148 (MQ=17)

                           ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCt                                              <  2:2189759/148‑1 (MQ=17)

                           ttaAAGAAGCGTTCTTTGCAGGGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCt                                              <  2:3293873/148‑1 (MQ=17)

                            taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTa                                             >  2:231999/1‑148 (MQ=17)

                             aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAg                                            >  1:179146/1‑148 (MQ=17)

                               agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAggg                                          >  1:3265821/1‑148 (MQ=17)

                               agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAggg                                          >  2:1695793/1‑148 (MQ=17)

                               agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAggg                                          <  2:264879/148‑1 (MQ=17)

                                gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAgggg                                         >  1:1269009/1‑148 (MQ=17)

                                gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAgggg                                         >  1:1881717/1‑148 (MQ=17)

                                gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAgggg                                         >  1:1440213/1‑148 (MQ=17)

                                gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAgggg                                         <  1:3310709/148‑1 (MQ=17)

                                gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAgggg                                         >  1:2950293/1‑148 (MQ=17)

                                 aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGa                                        >  2:1605169/1‑148 (MQ=17)

                                 aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGa                                        >  2:3242571/1‑148 (MQ=17)

                                 aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGa                                        <  2:3291770/148‑1 (MQ=17)

                                  agCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGAc                                       >  2:203696/1‑148 (MQ=17)

                                   gCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACg                                      >  1:675389/1‑148 (MQ=17)

                                    cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGc                                     >  1:2122055/1‑148 (MQ=17)

                                    cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGc                                     >  2:130887/1‑148 (MQ=17)

                                    cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGc                                     >  2:2736738/1‑148 (MQ=17)

                                    cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGc                                     >  1:1856582/1‑148 (MQ=17)

                                      ttCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCa                                   >  1:2394402/1‑148 (MQ=17)

                                      ttCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCa                                   >  1:2394475/1‑148 (MQ=17)

                                       tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCAc                                  >  2:57515/1‑148 (MQ=32)

                                       tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCAc                                  <  1:1536085/148‑1 (MQ=17)

                                       tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCAc                                  >  1:1098526/1‑148 (MQ=17)

                                       tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCAc                                  <  1:1534883/148‑1 (MQ=17)

                                        cTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACt                                 >  2:718473/1‑148 (MQ=17)

                                          ttGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTg                               >  2:751774/1‑148 (MQ=17)

                                           tGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGc                              <  2:2148230/148‑1 (MQ=17)

                                               gTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGt                          >  2:2425234/1‑148 (MQ=17)

                                                tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCTTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTATAACTGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGACACTTGCTGGtt                         >  1:1001273/1‑148 (MQ=11)

                                                tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTCGGGGACGCCACTTGCTGGtt                         >  1:1464271/1‑148 (MQ=17)

                                                tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                         >  1:1001576/1‑148 (MQ=17)

                                                tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                         <  1:2243364/148‑1 (MQ=17)

                                                tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGtt                         >  1:1094897/1‑148 (MQ=17)

                                                 gCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGttt                        <  1:1005682/148‑1 (MQ=17)

                                                   tCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtgt                      <  1:1596671/148‑1 (MQ=17)

                                                   tCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtgt                      >  1:1847572/1‑148 (MQ=17)

                                                   tCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtgt                      <  1:2071687/148‑1 (MQ=17)

                                                    cacacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtgtg                     >  1:2523539/1‑148 (MQ=32)

                                                    cacacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtgtg                     >  1:1395032/1‑148 (MQ=17)

                                                      cacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag                   >  2:3204412/1‑148 (MQ=32)

                                                      cacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag                   >  1:352704/1‑148 (MQ=17)

                                                      cacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag                   >  2:1662572/1‑148 (MQ=17)

                                                       acaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagt                  <  1:1662572/148‑1 (MQ=17)

                                                       acaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagt                  <  1:1454879/148‑1 (MQ=17)

                                                          gATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaa               <  1:1094402/148‑1 (MQ=17)

                                                           aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaaa              >  1:545768/1‑148 (MQ=32)

                                                           aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaaa              <  2:2492466/148‑1 (MQ=17)

                                                            ttGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAg             >  1:3024612/1‑148 (MQ=17)

                                                            ttGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAg             >  1:3191367/1‑148 (MQ=17)

                                                             tGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGt            >  1:96562/1‑148 (MQ=17)

                                                               tCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCa          >  2:2824612/1‑148 (MQ=17)

                                                                cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAc         <  1:2185684/148‑1 (MQ=17)

                                                                cTGATAGGAAGTGAAAAGCAAGGCGTCGTGCGAAGCAGACTGATACGTCCCCGTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAc         <  2:2407879/148‑1 (MQ=17)

                                                                   aTAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTg      >  1:109402/1‑148 (MQ=17)

                                                                     aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGcc    >  2:3063665/1‑148 (MQ=17)

                                                                     aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGcc    >  2:3063396/1‑148 (MQ=17)

                                                                     aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGcc    <  1:2613412/148‑1 (MQ=17)

                                                                      ggAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTAGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCt   >  2:769074/1‑148 (MQ=14)

                                                                      ggAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTAGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCt   >  2:769108/1‑148 (MQ=14)

                                                                      ggAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTAGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCt   >  2:771019/1‑148 (MQ=14)

                                                                       gAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTAACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCtt  >  1:964519/1‑148 (MQ=11)

AACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTT  >  CP009273/3936685‑3936903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`