Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,959,351 G→T 91.0% P77P (CCG→CCT)  trxA → thioredoxin 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,959,3510GT91.0% 582.5 / 50.4 219P77P (CCG→CCT) trxAthioredoxin 1
Reads supporting (aligned to +/- strand):  ref base G (10/10);  new base T (114/85);  total (124/95)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

GGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGCCCGG  >  CP009273/3959207‑3959497
                                                                                                                                                |                                                                                                                                                  
ggCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTAct                                                                                                                                                 <  1:1986413/148‑1 (MQ=255)
ggCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTAct                                                                                                                                                 <  2:2495604/148‑1 (MQ=255)
ggCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTAct                                                                                                                                                 <  1:389127/148‑1 (MQ=255)
   agagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCTAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctg                                                                                                                                              <  1:3234404/148‑1 (MQ=255)
    gagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgc                                                                                                                                             >  1:119046/1‑148 (MQ=255)
             ggTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCa                                                                                                                                    >  2:2751177/1‑148 (MQ=255)
                ccGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaaaa                                                                                                                                 <  1:980650/148‑1 (MQ=255)
                 cGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaaaaa                                                                                                                                <  1:2552192/148‑1 (MQ=255)
                   tGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACg                                                                                                                              <  2:1854053/148‑1 (MQ=255)
                   tGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACg                                                                                                                              <  1:2449826/148‑1 (MQ=255)
                     cAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGt                                                                                                                            <  2:1619018/148‑1 (MQ=255)
                     cAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGt                                                                                                                            <  1:302866/148‑1 (MQ=255)
                        aaTGATCGCCCCTATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGaa                                                                                                                         >  1:1433423/1‑148 (MQ=255)
                        aaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGaa                                                                                                                         <  1:2051583/148‑1 (MQ=255)
                        aaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGaa                                                                                                                         <  1:1019835/148‑1 (MQ=255)
                          tGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGt                                                                                                                       <  2:682188/148‑1 (MQ=255)
                          tGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATAAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGt                                                                                                                       >  1:2336828/1‑148 (MQ=255)
                             tCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAAc                                                                                                                               >  1:479906/1‑137 (MQ=255)
                             tCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTggc                                                                                                                    >  1:3170194/1‑148 (MQ=255)
                                 cccGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCa                                                                                                                >  1:1237929/1‑148 (MQ=255)
                                   cGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAAc                                                                                                              >  2:3001531/1‑148 (MQ=255)
                                       tCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCaaa                                                                                                          >  2:52907/1‑148 (MQ=255)
                                       tCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCaaa                                                                                                          <  1:251908/148‑1 (MQ=255)
                                         tGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGt                                                                                                        >  1:746963/1‑148 (MQ=255)
                                          ggATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTg                                                                                                       <  2:2557765/148‑1 (MQ=255)
                                          ggATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTg                                                                                                       <  1:942542/148‑1 (MQ=255)
                                            aTGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTggg                                                                                                     >  2:471162/1‑148 (MQ=255)
                                                  tCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACt                                                                                               >  1:1530304/1‑148 (MQ=255)
                                                  tCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACt                                                                                               <  1:594757/148‑1 (MQ=255)
                                                     cTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTc                                                                                            <  2:468227/148‑1 (MQ=255)
                                                       gACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTa                                                                                          >  2:2013961/1‑148 (MQ=255)
                                                        aCGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaa                                                                                         >  2:2058576/1‑148 (MQ=255)
                                                        aCGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaa                                                                                         >  2:279930/1‑148 (MQ=255)
                                                         cGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaaa                                                                                        >  1:373236/1‑148 (MQ=255)
                                                         cGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaaa                                                                                        >  1:1358973/1‑148 (MQ=255)
                                                         cGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaaa                                                                                        >  1:1358802/1‑148 (MQ=255)
                                                            atatCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGt                                                                                     <  1:2688592/148‑1 (MQ=255)
                                                             tatCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTc                                                                                    >  1:3274582/1‑148 (MQ=255)
                                                              atCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCa                                                                                   <  2:1023449/148‑1 (MQ=255)
                                                               tCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAg                                                                                  <  2:1348046/148‑1 (MQ=255)
                                                               tCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAg                                                                                  <  2:282917/148‑1 (MQ=255)
                                                                 aGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGtt                                                                                >  2:402972/1‑148 (MQ=255)
                                                                   ggCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGa                                                                              >  1:3170835/1‑148 (MQ=255)
                                                                      aaaCTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAag                                                                           <  2:2429441/148‑1 (MQ=255)
                                                                       aaCTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAaga                                                                          <  2:1126687/148‑1 (MQ=255)
                                                                       aaCTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAaga                                                                          <  2:3162210/148‑1 (MQ=255)
                                                                       aaCTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAaga                                                                          >  2:3268301/1‑148 (MQ=255)
                                                                        aCTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAagag                                                                         >  2:920607/1‑148 (MQ=255)
                                                                         cTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGt                                                                        >  1:1967789/1‑148 (MQ=255)
                                                                           gACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTc                                                                      <  1:2361327/148‑1 (MQ=255)
                                                                             ccGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCt                                                                    <  2:120450/148‑1 (MQ=255)
                                                                             ccGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCt                                                                    <  1:11951/148‑1 (MQ=255)
                                                                                ttGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGa                                                                 >  2:1979444/1‑148 (MQ=255)
                                                                                 tGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGTGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGAc                                                                >  1:748446/1‑148 (MQ=255)
                                                                                 tGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGAc                                                                >  1:564213/1‑148 (MQ=255)
                                                                                 tGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGAc                                                                >  1:1081582/1‑148 (MQ=255)
                                                                                  gCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACg                                                               <  1:1084520/148‑1 (MQ=255)
                                                                                       acataaCATCGATCAAAACCATTGCACTGCTACGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGCTGAAGTGGAGGCAACCAAAGTGGGTGCACTGACTAAAGTTCAGTTGAAAGAGTTCATCGACGCTAAc                                                          >  2:1476627/5‑148 (MQ=255)
                                                                                       aCTGAACATCTATCAAAACCCTGGCACTGCGCAGAAATATGGCATCAGTGGTAACCCGACTCTGCTGCTGTTCAAAAACTGTGAAGTTGCGGCAACCAAATTGTGTGCACTGTCTAAAGTTCAGTTGAAATAGTTCCTCGACGCTAAc                                                          >  2:1237880/1‑148 (MQ=255)
                                                                                        cTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAAcc                                                         <  2:1589030/148‑1 (MQ=255)
                                                                                        cTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAAcc                                                         >  1:2877891/1‑148 (MQ=255)
                                                                                        cTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAAcc                                                         >  2:850310/1‑148 (MQ=255)
                                                                                         tGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCt                                                        <  2:1637289/148‑1 (MQ=255)
                                                                                           aaCATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTgg                                                      >  1:518342/1‑148 (MQ=255)
                                                                                             cATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCg                                                    >  2:392457/1‑148 (MQ=255)
                                                                                              aTCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGt                                                   <  2:1871391/148‑1 (MQ=255)
                                                                                              aTCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGt                                                   >  2:937509/1‑148 (MQ=255)
                                                                                                cGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTaa                                                 >  2:2378448/1‑148 (MQ=255)
                                                                                                cGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTaa                                                 <  1:1629322/148‑1 (MQ=255)
                                                                                                   tCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAggg                                              >  2:2537646/1‑148 (MQ=255)
                                                                                                    cAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGa                                             >  2:2807699/1‑148 (MQ=255)
                                                                                                      aaaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAt                                           >  2:888052/1‑148 (MQ=255)
                                                                                                      aaaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAt                                           >  1:725516/1‑148 (MQ=255)
                                                                                                       aaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAtt                                          >  2:2238073/1‑148 (MQ=255)
                                                                                                       aaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAtt                                          <  1:3091784/148‑1 (MQ=255)
                                                                                                        aCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAttt                                         <  2:945332/148‑1 (MQ=255)
                                                                                                         cccTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTc                                        >  2:122470/1‑148 (MQ=255)
                                                                                                          ccTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCa                                       <  2:865030/148‑1 (MQ=255)
                                                                                                           cTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCAt                                      <  2:199506/148‑1 (MQ=255)
                                                                                                            tGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATg                                     <  1:2886683/148‑1 (MQ=255)
                                                                                                             ggCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGt                                    >  2:304913/1‑148 (MQ=255)
                                                                                                                         aaaTATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCg                        >  2:1926524/1‑148 (MQ=255)
                                                                                                                          aaTATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGt                       <  1:2783080/148‑1 (MQ=255)
                                                                                                                          aaTATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGt                       >  2:2362138/1‑148 (MQ=255)
                                                                                                                           atatGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTc                      >  2:979964/1‑148 (MQ=255)
                                                                                                                           atatGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTc                      >  2:566858/1‑148 (MQ=255)
                                                                                                                           atatGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTc                      >  2:1169606/1‑148 (MQ=255)
                                                                                                                             atGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGc                    >  2:1302753/1‑148 (MQ=255)
                                                                                                                                gCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaa                 >  1:3010711/1‑148 (MQ=255)
                                                                                                                                   tCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaaaaa              <  1:506207/148‑1 (MQ=255)
                                                                                                                                     cGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACt            <  1:710507/148‑1 (MQ=255)
                                                                                                                                       tGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTgg          <  2:623100/148‑1 (MQ=255)
                                                                                                                                        ggTATCCCTTCTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAATGTGGGTGCACTGTCTTAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGATTTTCATGTTCGGGTGCCCCGTCGATAAAAACTggt         <  2:3068322/148‑2 (MQ=255)
                                                                                                                                         gTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGAc        >  1:2286641/1‑148 (MQ=255)
                                                                                                                                            tCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGcc     <  2:72349/148‑1 (MQ=255)
                                                                                                                                            tCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGcc     >  1:1823426/1‑148 (MQ=255)
                                                                                                                                             cccTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGccc    >  2:2600240/1‑148 (MQ=255)
                                                                                                                                             cccTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGccc    >  2:2597476/1‑148 (MQ=255)
                                                                                                                                             cccTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGccc    >  2:813493/1‑148 (MQ=255)
                                                                                                                                             cccTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGccc    >  2:1747037/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGCCCGG  >  CP009273/3959207‑3959497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑