Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,966,854 C→A 100% R325S (CGT→AGT)  rffG → dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,966,8540CA99.0% 634.4 / ‑1.7 194R325S (CGT→AGT) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (100/91);  total (102/92)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 194 total aligned reads displayed.

AAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCG  >  CP009273/3966710‑3966998
                                                                                                                                                |                                                                                                                                                
   ccGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaaaa                                                                                                                                            <  2:268358/148‑1 (MQ=255)
   ccGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTaaaa                                                                                                                                            <  1:956790/148‑1 (MQ=255)
      cACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACgg                                                                                                                                         <  1:2586260/148‑1 (MQ=255)
      cACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACgg                                                                                                                                         <  1:3219866/148‑1 (MQ=255)
      cACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACgg                                                                                                                                         >  2:2777789/1‑148 (MQ=255)
      cACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACgg                                                                                                                                         <  1:1770574/148‑1 (MQ=255)
          gCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCa                                                                                                                                     <  1:229590/148‑1 (MQ=255)
           cGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAg                                                                                                                                    >  2:3054746/1‑148 (MQ=255)
               gcgcACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGt                                                                                                                                <  2:2232970/148‑1 (MQ=255)
                cgcACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTa                                                                                                                               <  1:981949/148‑1 (MQ=255)
                  cACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATc                                                                                                                             <  2:1395905/148‑1 (MQ=255)
                   aCTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCt                                                                                                                            >  2:2912844/1‑148 (MQ=255)
                       tCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCt                                                                                                                        >  1:563990/1‑148 (MQ=255)
                        cGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTa                                                                                                                       <  2:2347611/148‑1 (MQ=255)
                            aCCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGa                                                                                                                   >  1:1031551/1‑148 (MQ=255)
                            aCCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGa                                                                                                                   >  1:1031555/1‑148 (MQ=255)
                                  tCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtg                                                                                                             <  2:97216/148‑1 (MQ=255)
                                   cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                                                                                                            <  2:1047830/148‑1 (MQ=255)
                                   cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                                                                                                            <  2:70018/148‑1 (MQ=255)
                                   cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                                                                                                            <  1:1736894/148‑1 (MQ=255)
                                   cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                                                                                                            <  1:1841072/148‑1 (MQ=255)
                                     ccTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggtg                                                                                                          >  2:1457377/1‑148 (MQ=255)
                                     ccTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggtg                                                                                                          >  2:2033573/1‑148 (MQ=255)
                                      cTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggtgg                                                                                                         >  2:297022/1‑148 (MQ=255)
                                           tCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCa                                                                                                    <  1:2656612/148‑1 (MQ=255)
                                           tCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCa                                                                                                    >  1:3255808/1‑148 (MQ=255)
                                             gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAgg                                                                                                  <  1:342464/148‑1 (MQ=255)
                                              cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGt                                                                                                 >  1:2219612/1‑148 (MQ=255)
                                              cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGt                                                                                                 >  1:3269641/1‑148 (MQ=255)
                                                 aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCa                                                                                              >  1:2061270/1‑148 (MQ=255)
                                                 aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCa                                                                                              >  1:1940482/1‑148 (MQ=255)
                                                 aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCa                                                                                              >  1:396078/1‑148 (MQ=255)
                                                 aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCa                                                                                              >  1:30429/1‑148 (MQ=255)
                                                  ccGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAg                                                                                             >  2:1992056/1‑148 (MQ=255)
                                                  ccGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAg                                                                                             >  2:1992245/1‑148 (MQ=255)
                                                     tCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAc                                                                                          >  2:625936/1‑148 (MQ=255)
                                                      ccGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAACTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGTCTAATGAAAGCTGGTGGAAGAAGGTGCAGGACg                                                                                         >  1:1786096/1‑148 (MQ=255)
                                                         gggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCa                                                                                      >  1:528713/1‑148 (MQ=255)
                                                            cATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCt                                                                                   <  1:2551847/148‑1 (MQ=255)
                                                            cATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCt                                                                                   >  2:254018/1‑148 (MQ=255)
                                                                 tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAg                                                                              >  1:118980/1‑148 (MQ=255)
                                                                 tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAg                                                                              >  2:1305473/1‑148 (MQ=255)
                                                                 tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAg                                                                              <  1:3207719/148‑1 (MQ=255)
                                                                  cTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAgg                                                                             >  2:2494990/1‑148 (MQ=255)
                                                                   tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAggg                                                                            <  1:2121802/148‑1 (MQ=255)
                                                                   tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAggg                                                                            <  1:1634349/148‑1 (MQ=255)
                                                                    gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTAt                                                                                 >  2:2629436/1‑142 (MQ=255)
                                                                    gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTAt                                                                                 <  1:604669/142‑1 (MQ=255)
                                                                    gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTAt                                                                                 >  2:604669/1‑142 (MQ=255)
                                                                    gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGc                                                                           >  1:2355298/1‑148 (MQ=255)
                                                                      gTTAGGCCATTGATGCTTCGAAAATTGCCCGTGAACGTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGa                                                                         <  1:4261/148‑1 (MQ=255)
                                                                        tATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGc                                                                       >  1:1931992/1‑148 (MQ=255)
                                                                           gCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGtt                                                                    >  2:2107367/1‑148 (MQ=255)
                                                                           gCCATTGATGCTTCGAAAATTGCCCGTGAACTGGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGtt                                                                    <  1:2889154/148‑1 (MQ=255)
                                                                            ccATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGttt                                                                   >  2:1143002/1‑148 (MQ=255)
                                                                              aTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTGTAg                                                                 >  2:1856179/1‑148 (MQ=255)
                                                                                tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGt                                                               >  1:1345525/1‑148 (MQ=255)
                                                                                tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGt                                                               >  2:253460/1‑148 (MQ=255)
                                                                                tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGt                                                               >  2:1585512/1‑148 (MQ=255)
                                                                                tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGt                                                               >  2:1585550/1‑148 (MQ=255)
                                                                                tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAACCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGt                                                               <  1:3068730/148‑1 (MQ=255)
                                                                                  aTGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCt                                                             >  1:1573202/1‑148 (MQ=255)
                                                                                     cTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGaa                                                          >  1:1228284/1‑148 (MQ=255)
                                                                                      ttCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGaaa                                                         >  2:2418238/1‑148 (MQ=255)
                                                                                       tCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAg                                                        >  2:2327568/1‑148 (MQ=255)
                                                                                          aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCt                                                     >  1:1865709/1‑148 (MQ=255)
                                                                                           aaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTa                                                    <  2:55499/148‑1 (MQ=255)
                                                                                           aaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTa                                                    >  1:333395/1‑148 (MQ=255)
                                                                                           aaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTa                                                    <  2:2519410/148‑1 (MQ=255)
                                                                                           aaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAAGGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTa                                                    <  2:2536094/148‑1 (MQ=255)
                                                                                              ttGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAAtt                                                 >  1:940933/1‑148 (MQ=255)
                                                                                               tGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAAttt                                                <  2:2699103/148‑1 (MQ=255)
                                                                                                gCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAAtttt                                               <  1:2709149/148‑1 (MQ=255)
                                                                                                     tGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCg                                          <  2:876390/148‑1 (MQ=255)
                                                                                                      gAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCgg                                         >  1:774087/1‑148 (MQ=255)
                                                                                                       aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGa                                        <  2:1774709/148‑1 (MQ=255)
                                                                                                       aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGa                                        <  2:2057993/148‑1 (MQ=255)
                                                                                                       aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGa                                        <  2:430542/148‑1 (MQ=255)
                                                                                                         cTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGACTGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAgg                                      >  1:535779/1‑148 (MQ=255)
                                                                                                          ttGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGc                                     >  2:2070674/1‑148 (MQ=255)
                                                                                                                 gctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATg                              >  2:1175965/1‑148 (MQ=255)
                                                                                                                 gctgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATg                              <  2:1916516/148‑1 (MQ=255)
                                                                                                                   tgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGaa                            <  2:2620613/148‑1 (MQ=255)
                                                                                                                   tgCCGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGaa                            <  1:1295349/148‑1 (MQ=255)
                                                                                                                     ccGCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAg                          <  2:494438/148‑1 (MQ=255)
                                                                                                                       gCAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGt                        <  2:3181829/148‑1 (MQ=255)
                                                                                                                        cAGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGta                       >  2:860765/1‑148 (MQ=255)
                                                                                                                         aGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtat                      <  1:1143002/148‑1 (MQ=255)
                                                                                                                         aGGAAACCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtat                      <  2:271368/148‑1 (MQ=255)
                                                                                                                            aaaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGtattat                   <  1:895369/148‑1 (MQ=255)
                                                                                                                             aaCCTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATc                  <  1:1500885/148‑1 (MQ=255)
                                                                                                                               ccTTTGAAAGTGGAATGAGTAACACGGTTCAGTGTTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCt                <  1:3085409/148‑1 (MQ=255)
                                                                                                                               ccTTTGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCt                >  1:370125/1‑148 (MQ=255)
                                                                                                                                 tttGAAAGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTgg              >  1:959268/1‑148 (MQ=255)
                                                                                                                                      aaGTGGAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTggcgggc         >  1:839785/1‑148 (MQ=255)
                                                                                                                                          ggAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGtt     <  2:805677/148‑1 (MQ=255)
                                                                                                                                           gAATGAGTAACACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTc    <  1:2505372/148‑1 (MQ=255)
                                                                                                                                           gAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTc    <  1:1439809/148‑1 (MQ=255)
                                                                                                                                           gAATGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTc    >  2:1395406/1‑148 (MQ=255)
                                                                                                                                            aaTGAGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTcc   >  2:1214394/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AAGCCGCACGGCGTGGCGCACTATCGTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGGCAGCTATCAGGGCGAGCGTTTAGGTCTGAAAGGCTAATTTTCAGCGGAGGCAAACATGAAAGGTATTATCCTGGCGGGCGGTTCCG  >  CP009273/3966710‑3966998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑