BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,001,435	G→T	12.8%	E107* (GAG→TAG)	rhtC →	threonine efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,001,435	0	G	T	12.8%	598.1 / 44.5	184	E107* (GAG→TAG)	rhtC	threonine efflux pump
Reads supporting (aligned to +/- strand): ref base G (86/74); new base T (9/15); total (95/89)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

CTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGA  >  CP009273/4001288‑4001582

ctTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCg                                                                                                                                                     <  2:299957/148‑1 (MQ=255)

  tGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAg                                                                                                                                                   >  1:901130/1‑148 (MQ=255)

  tGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAg                                                                                                                                                   >  2:157225/1‑148 (MQ=255)

        gCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCg                                                                                                                                             >  2:1236299/1‑148 (MQ=255)

         cATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCTAGCTGGCGa                                                                                                                                            >  2:2792891/1‑148 (MQ=255)

          aTTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGaa                                                                                                                                           >  1:1491047/1‑148 (MQ=255)

           tttGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGACTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGACAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGaaa                                                                                                                                          >  2:2467812/1‑148 (MQ=255)

            ttGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGaaaa                                                                                                                                         >  1:3256270/1‑148 (MQ=255)

            ttGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGaaaa                                                                                                                                         <  1:986349/148‑1 (MQ=255)

             tGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAg                                                                                                                                        <  1:3171762/148‑1 (MQ=255)

              gATTATCTAAAAATTGGCCTGTCTGCATACTCTGATTATGGTGGGCGGTGGACTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGt                                                                                                                                       <  1:2782252/148‑1 (MQ=255)

              gATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGt                                                                                                                                       <  1:449737/148‑1 (MQ=255)

                ttATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTgg                                                                                                                                     >  2:2585202/1‑148 (MQ=255)

                 tATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGTCGAAAAGTggg                                                                                                                                    >  2:2731698/1‑148 (MQ=255)

                 tATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTtgg                                                                                                                                    >  2:1832285/1‑148 (MQ=255)

                 tATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTggg                                                                                                                                    >  1:2639680/1‑148 (MQ=255)

                 tATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTggg                                                                                                                                    >  2:804091/1‑148 (MQ=255)

                 tATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTggg                                                                                                                                    >  1:1717651/1‑148 (MQ=255)

                 tATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTggg                                                                                                                                    >  1:39195/1‑148 (MQ=255)

                 tATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTggg                                                                                                                                    >  2:160847/1‑148 (MQ=255)

                    cGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGgcgc                                                                                                                                 >  2:173916/1‑148 (MQ=255)

                      aaaaaaTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAg                                                                                                                               >  1:2035503/1‑148 (MQ=255)

                       aaaaaTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGt                                                                                                                              >  2:800638/1‑148 (MQ=255)

                          aaTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTc                                                                                                                           >  1:795067/1‑148 (MQ=255)

                           aTGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTcc                                                                                                                          >  1:2757230/1‑148 (MQ=255)

                               ccTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGaa                                                                                                                      <  1:1901818/148‑1 (MQ=255)

                                 tGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAg                                                                                                                    >  2:1809843/1‑148 (MQ=255)

                                  ggCTGCATACGCTTATTATGGTGGGCGGTGGCCTGTTTCTCTGCTGGATGGGTTACCAGATTCTTCGTGGTGCACTGAAAAAAGAGGCGCTTTCTGCACCTGCGCCTCAGTTCTAGCTTGCGAAAAGTGTGCGCTGTTTCCTGAAAgt                                                                                                                   <  2:208094/148‑2 (MQ=255)

                                  ggCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAgg                                                                                                                   <  1:441957/148‑1 (MQ=255)

                                   gCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGt                                                                                                                  <  1:255634/148‑1 (MQ=255)

                                   gCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGt                                                                                                                  >  1:1791931/1‑148 (MQ=255)

                                    cTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCTAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGtt                                                                                                                 >  1:379756/1‑148 (MQ=255)

                                    cTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGtt                                                                                                                 >  2:1080525/1‑148 (MQ=255)

                                       cATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCTAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTAc                                                                                                              <  1:685856/148‑1 (MQ=255)

                                         tACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACtt                                                                                                            >  2:1580280/1‑147 (MQ=255)

                                          aCGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGa                                                                                                           <  1:2870684/148‑1 (MQ=255)

                                          aCGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGa                                                                                                           >  2:793389/1‑148 (MQ=255)

                                              tGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCaa                                                                                                       >  1:259995/1‑148 (MQ=255)

                                               gATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAAt                                                                                                      <  1:1529045/148‑1 (MQ=255)

                                                 ttATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAAtct                                                                                                    >  1:1457059/1‑148 (MQ=255)

                                                    tggtggGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGc                                                                                                 >  1:239568/1‑148 (MQ=255)

                                                     ggtggGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCt                                                                                                >  2:250483/1‑148 (MQ=255)

                                                      gtggGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTa                                                                                               <  1:1473634/148‑1 (MQ=255)

                                                       tggGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCTAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTaa                                                                                              <  1:2852903/148‑1 (MQ=255)

                                                       tggGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCTAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTaa                                                                                              <  1:1203353/148‑1 (MQ=255)

                                                         ggCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATc                                                                                            >  2:842995/1‑148 (MQ=255)

                                                            ggTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGa                                                                                         <  2:2317225/148‑1 (MQ=255)

                                                               ggCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAg                                                                                      <  1:1300495/148‑1 (MQ=255)

                                                                gCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGc                                                                                     >  1:3312614/1‑148 (MQ=255)

                                                                 ccTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCg                                                                                    <  1:585397/148‑1 (MQ=255)

                                                                 ccTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCg                                                                                    >  2:408040/1‑148 (MQ=255)

                                                                 ccTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCg                                                                                    <  1:425908/148‑1 (MQ=255)

                                                                  cTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGa                                                                                   >  1:2824513/1‑148 (MQ=255)

                                                                      aTCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTAt                                                                               >  1:1360707/1‑148 (MQ=255)

                                                                        ctctGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCt                                                                             <  2:1766997/148‑1 (MQ=255)

                                                                        ctctGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCt                                                                             <  1:173916/148‑1 (MQ=255)

                                                                        ctctGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCt                                                                             >  2:3106108/1‑148 (MQ=255)

                                                                         tctGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTa                                                                            >  2:449319/1‑148 (MQ=255)

                                                                             ctgGATGGGTTACCAGATGCTACGTGGTGCACTGACAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACttt                                                                        <  2:2043702/148‑1 (MQ=255)

                                                                             ctgGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACttt                                                                        <  2:1632090/148‑1 (MQ=255)

                                                                               ggATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTgg                                                                      <  2:334115/148‑1 (MQ=255)

                                                                                 aTGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCt                                                                    >  1:3129575/1‑148 (MQ=255)

                                                                                      ttACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGgtg                                                               <  1:53801/148‑1 (MQ=255)

                                                                                          cAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGAGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGTCTCGGTGTtct                                                           >  2:1417901/1‑148 (MQ=255)

                                                                                           aGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTtctc                                                          >  1:3233174/1‑148 (MQ=255)

                                                                                           aGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTtctc                                                          >  1:305006/1‑148 (MQ=255)

                                                                                           aGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTtctc                                                          >  1:307287/1‑148 (MQ=255)

                                                                                            gATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCa                                                         >  1:2047889/1‑148 (MQ=255)

                                                                                             aTGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCAt                                                        >  1:815201/1‑148 (MQ=255)

                                                                                                cTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCTAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCAttgt                                                     <  2:2942081/148‑1 (MQ=255)

                                                                                                cTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCAttgt                                                     >  2:2661837/1‑148 (MQ=255)

                                                                                                cTACGTGGGGCACTGAAATAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCTAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCAttgt                                                     <  2:2942179/148‑1 (MQ=255)

                                                                                                  aCGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCCCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCAttgttt                                                   <  1:551919/148‑1 (MQ=255)

                                                                                                    gtggtgCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCAttgtttgt                                                 <  1:426139/148‑1 (MQ=255)

                                                                                                    gtggtgCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCAttgtttgt                                                 <  1:425963/148‑1 (MQ=255)

                                                                                                         gCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTg                                            <  2:1794461/148‑1 (MQ=255)

                                                                                                            cTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATa                                         >  2:2560749/1‑148 (MQ=255)

                                                                                                             tGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATaa                                        <  1:1335021/148‑1 (MQ=255)

                                                                                                             tGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATaa                                        <  1:1350063/148‑1 (MQ=255)

                                                                                                             tGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATaa                                        <  1:2894979/148‑1 (MQ=255)

                                                                                                                  aaaGAGGCGGTTTCTGCACCTGCGCCACAGGTCTAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTg                                   >  1:2716887/1‑148 (MQ=255)

                                                                                                                   aaGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTgg                                  <  1:346994/148‑1 (MQ=255)

                                                                                                                      agGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCAc                               >  1:1018600/1‑148 (MQ=255)

                                                                                                                      agGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCAc                               >  1:1017673/1‑148 (MQ=255)

                                                                                                                        gCGGTTTCTGCACCTGCGCCACAGGTCTAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTa                             <  1:28595/148‑1 (MQ=255)

                                                                                                                            tttCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACcgc                         >  1:1276224/1‑148 (MQ=255)

                                                                                                                             ttCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACcgcg                        >  1:784764/1‑148 (MQ=255)

                                                                                                                               cTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACcgcgcg                      <  2:1491047/148‑1 (MQ=255)

                                                                                                                               cTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACcgcgcg                      <  1:119700/148‑1 (MQ=255)

                                                                                                                                 gCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCt                    >  1:1597610/1‑148 (MQ=255)

                                                                                                                                 gCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCt                    >  2:277463/1‑148 (MQ=255)

                                                                                                                                     cTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTgggg                >  1:3246219/1‑148 (MQ=255)

                                                                                                                                        cgcCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCAt             <  1:2233803/148‑1 (MQ=255)

                                                                                                                                          ccACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCAttt           <  2:676648/148‑1 (MQ=255)

                                                                                                                                           cacaGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCAtttt          >  1:1802371/1‑148 (MQ=255)

                                                                                                                                             caGGTCTAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTg        >  1:2068989/1‑148 (MQ=255)

                                                                                                                                             caGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTg        <  2:2111166/148‑1 (MQ=255)

                                                                                                                                                gTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTgcgc     >  1:1997658/1‑148 (MQ=255)

                                                                                                                                                  cGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTg   >  1:2102455/1‑148 (MQ=255)

                                                                                                                                                  cGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTg   >  2:1379332/1‑148 (MQ=255)

CTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGCGAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGTTCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTTGCGCTGA  >  CP009273/4001288‑4001582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`