Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 332,599 G→T 89.9% A37A (GCG→GCT)  yahF → putative NAD(P)‑binding succinyl‑CoA synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273332,5990GT89.9% 531.3 / 52.5 206A37A (GCG→GCT) yahFputative NAD(P)‑binding succinyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base G (10/11);  new base T (82/103);  total (92/114)
Fisher's exact test for biased strand distribution p-value = 8.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

ATGGTTGTGCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCGATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGATTGA  >  CP009273/332453‑332742
                                                                                                                                                  |                                                                                                                                               
aTGGTTGTGCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGtggcga                                                                                                                                                >  2:1059191/1‑148 (MQ=255)
  ggTTGTGCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGtggcgatg                                                                                                                                              >  1:1712914/1‑148 (MQ=255)
  ggTTGTGCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGgggctatg                                                                                                                                              <  2:1723542/148‑1 (MQ=255)
    ttGTGCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGtggctatggc                                                                                                                                            >  1:2854693/1‑148 (MQ=255)
      gtgCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGtggctatggcga                                                                                                                                          <  1:780006/148‑1 (MQ=255)
       tgCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGAc                                                                                                                                         <  1:3231670/148‑1 (MQ=255)
        gcgcTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGAcc                                                                                                                                        <  1:2326859/148‑1 (MQ=255)
         cgcTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCg                                                                                                                                       >  2:3005895/1‑148 (MQ=255)
          gcTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGa                                                                                                                                      <  2:655081/148‑1 (MQ=255)
            tGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGaaa                                                                                                                                    <  1:2617099/148‑1 (MQ=255)
            tGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGaaa                                                                                                                                    <  1:462810/148‑1 (MQ=255)
              aGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATg                                                                                                                                  >  1:2131025/1‑148 (MQ=255)
               gCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGa                                                                                                                                 <  1:2180958/148‑1 (MQ=255)
               gCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGa                                                                                                                                 <  1:1967239/148‑1 (MQ=255)
               gCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGa                                                                                                                                 <  1:697311/148‑1 (MQ=255)
               gCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGa                                                                                                                                 <  1:518062/148‑1 (MQ=255)
                 cAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAAt                                                                                                                               >  2:2074047/1‑148 (MQ=255)
                  aaaCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATa                                                                                                                              >  1:1258101/1‑148 (MQ=255)
                  aaaCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATa                                                                                                                              >  1:1258237/1‑148 (MQ=255)
                    aCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCCTTTGTGGCTATGGCGACCGAAAGGAATaaa                                                                                                                            >  2:3306482/1‑148 (MQ=255)
                    aCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATaaa                                                                                                                            <  2:2275554/148‑1 (MQ=255)
                    aCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATaaa                                                                                                                            >  1:873576/1‑148 (MQ=255)
                    aCCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCGATGGCGACCGAAATGAATaaa                                                                                                                            >  1:347690/1‑148 (MQ=255)
                     ccTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAg                                                                                                                           <  1:1482185/148‑1 (MQ=255)
                     ccTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAg                                                                                                                           <  1:3125018/148‑1 (MQ=255)
                      cTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAgg                                                                                                                          >  1:37561/1‑148 (MQ=255)
                      cTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAgg                                                                                                                          >  1:333664/1‑148 (MQ=255)
                      cTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAgg                                                                                                                          <  2:1813852/148‑1 (MQ=255)
                        aCGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCGATGGCGACCGAAATGAATAAAGGCg                                                                                                                        >  1:175236/1‑148 (MQ=255)
                            aGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCt                                                                                                                    <  2:2908366/148‑1 (MQ=255)
                              ggAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTga                                                                                                                  <  1:672982/148‑1 (MQ=255)
                              ggAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTga                                                                                                                  <  2:1465865/148‑1 (MQ=255)
                               gAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTgaa                                                                                                                 >  1:561576/1‑148 (MQ=255)
                                    aTGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAAtt                                                                                                            >  2:649816/1‑148 (MQ=255)
                                     tGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAAttt                                                                                                           >  1:2349336/1‑148 (MQ=255)
                                      gTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTa                                                                                                          <  2:1514865/148‑1 (MQ=255)
                                      gTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTa                                                                                                          >  1:2232618/1‑148 (MQ=255)
                                       tCAGTTAAAATAGTCATTAAACCGAAGACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAg                                                                                                         <  2:326141/148‑1 (MQ=255)
                                        cAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAgg                                                                                                        >  2:884504/1‑148 (MQ=255)
                                         aGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGa                                                                                                       <  2:769602/148‑1 (MQ=255)
                                         aGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGa                                                                                                       <  1:2228812/148‑1 (MQ=255)
                                            tAAAATAGTCTTTAAAACTAATAACTTTTTTTATTCTGTCTCGCTGATGTCTATCTCCACGAGTTCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAATGCGTGCTGAAGAATTTAGGActg                                                                                                    <  1:2562088/148‑1 (MQ=255)
                                            tAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGActg                                                                                                    <  1:687232/148‑1 (MQ=255)
                                                 tAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGAc                                                                                               >  1:2387329/1‑148 (MQ=255)
                                                  aGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACg                                                                                              <  1:1332695/148‑1 (MQ=255)
                                                  aGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACg                                                                                              >  2:1016100/1‑148 (MQ=255)
                                                   gTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGc                                                                                             >  2:43136/1‑148 (MQ=255)
                                                      aTTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCgg                                                                                          <  1:2002677/148‑1 (MQ=255)
                                                      aTTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCgg                                                                                          >  2:1680759/1‑148 (MQ=255)
                                                        tAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAg                                                                                        <  1:1811811/148‑1 (MQ=255)
                                                        tAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAg                                                                                        >  2:477182/1‑148 (MQ=255)
                                                         aaaCCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGc                                                                                       <  2:873576/148‑1 (MQ=255)
                                                           aCCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTg                                                                                     >  2:1562556/1‑148 (MQ=255)
                                                            ccGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTgg                                                                                    >  2:1748277/1‑148 (MQ=255)
                                                                  aCCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAgg                                                                              >  2:842645/1‑148 (MQ=255)
                                                                  aCCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAgg                                                                              >  1:1072522/1‑148 (MQ=255)
                                                                   ccTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGc                                                                             >  2:2104991/1‑148 (MQ=255)
                                                                    cTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCg                                                                            >  2:2471336/1‑148 (MQ=255)
                                                                        tttGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCGATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGaaaa                                                                        >  1:2815995/1‑148 (MQ=255)
                                                                          tGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAAc                                                                      <  2:980708/148‑1 (MQ=255)
                                                                          tGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAAc                                                                      <  2:1446187/148‑1 (MQ=255)
                                                                          tGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAAc                                                                      <  2:1256312/148‑1 (MQ=255)
                                                                           gATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACg                                                                     >  1:2453181/1‑148 (MQ=255)
                                                                             ttCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGc                                                                   >  2:731449/1‑148 (MQ=255)
                                                                             ttCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGc                                                                   >  2:731383/1‑148 (MQ=255)
                                                                              tCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCg                                                                  >  2:1205192/1‑148 (MQ=255)
                                                                                tGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGAc                                                                <  2:3029901/148‑1 (MQ=255)
                                                                                  tctcGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCt                                                              >  1:2428504/1‑148 (MQ=255)
                                                                                      gCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCtgatg                                                          >  2:1632574/1‑148 (MQ=255)
                                                                                        tGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTgatgat                                                        <  2:3064961/148‑1 (MQ=255)
                                                                                         gATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCTTCGAGCAGGCTTTTGTGGCTATGGCGACCGAAATGAATAATGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGAtt                                                       <  1:817906/148‑1 (MQ=255)
                                                                                          aTGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTg                                                      >  2:2152612/1‑148 (MQ=255)
                                                                                          aTGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAAAGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTg                                                      >  1:1898213/1‑148 (MQ=255)
                                                                                             tCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCTTTTGTGGCGATGGCGACCGAAATTAATAAAGGCGTGCTTAAGAATTTAGGACTGCTGATGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGtca                                                   <  1:66020/148‑1 (MQ=255)
                                                                                              cTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGtcat                                                  >  1:1973868/1‑148 (MQ=255)
                                                                                               tATCTCCACGCGTTCAAATAAACTCGACGGCGTCTCGCAGGCATTTGGGGCTAGGGCGACCGAAATGAATAAAGGCTTGCTGACGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGtcatc                                                 <  1:2733865/148‑1 (MQ=255)
                                                                                                aTCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGtcatca                                                <  2:2574621/148‑1 (MQ=255)
                                                                                                  ctcCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAAt                                              <  2:1713770/148‑1 (MQ=255)
                                                                                                  ctcCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATCTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAAt                                              <  2:2761631/148‑1 (MQ=255)
                                                                                                   tcCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCTTTTGGGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAATAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATg                                             <  1:926342/148‑1 (MQ=255)
                                                                                                   tcCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATg                                             >  1:1987697/1‑148 (MQ=255)
                                                                                                    ccACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCTAAATTAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATgg                                            <  2:2854599/148‑1 (MQ=255)
                                                                                                      aCGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTa                                          <  2:2513506/148‑1 (MQ=255)
                                                                                                       cgcgTGCAACTAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTaa                                         <  2:2564982/148‑1 (MQ=255)
                                                                                                        gcgTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTaaa                                        <  1:1670545/148‑1 (MQ=255)
                                                                                                        gcgTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAAGGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTaaa                                        >  2:2461135/1‑148 (MQ=255)
                                                                                                           tGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCg                                     >  2:3044481/1‑148 (MQ=255)
                                                                                                             cAACTAACCTCCACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGTAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCggg                                   <  2:2086547/148‑1 (MQ=255)
                                                                                                             cAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCggg                                   <  1:2899915/148‑1 (MQ=255)
                                                                                                                 tAAACTCGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCg                               >  2:2226739/1‑148 (MQ=255)
                                                                                                                  aaaCTCGACGGCGTCGAGCAGGCATTTGGGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCgg                              <  1:1278578/148‑1 (MQ=255)
                                                                                                                       cGACGGCGTCGAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAAc                         >  1:298961/1‑148 (MQ=255)
                                                                                                                                 gAGCTGGCTTTTGTGGCTATGGCGACCTAAATGAATAAAGGCGTGCTGAAGATTTTCGGACTGCTGACGCCGGTGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTAc               <  1:884504/148‑1 (MQ=255)
                                                                                                                                 gAGCAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTAc               <  2:1258205/148‑1 (MQ=255)
                                                                                                                                    cAGGCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACtgg            >  2:1409855/1‑148 (MQ=255)
                                                                                                                                      ggCATTTGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACtggtg          >  1:1411479/1‑148 (MQ=255)
                                                                                                                                         aTTTGTGGCTATGGCGACCGAAATGAATAAAGGCGGGCTTAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGgag       <  1:1425270/148‑1 (MQ=255)
                                                                                                                                          tttGTGGCTATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAACACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGgaga      <  1:2036819/148‑1 (MQ=255)
                                                                                                                                           ttGTGGCGATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGAt     <  2:1262310/148‑1 (MQ=255)
                                                                                                                                           ttGTGGCGATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGAt     <  2:1420703/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ATGGTTGTGCGCTGAGCCAAACCTACGGAGGGAATTATGTCAGTTAAAATAGTCATTAAACCGAATACCTATTTTGATTCTGTCTCGCTGATGTCTATCTCCACGCGTGCAAATAAACTCGACGGCGTCGAGCAGGCATTTGTGGCGATGGCGACCGAAATGAATAAAGGCGTGCTGAAGAATTTAGGACTGCTGACGCCGGAGCTGGAGCAGGCGAAAAACGGCGACCTGATGATTGTCATCAATGGTAAATCGGGTGCGGACAACGAGCAGTTACTGGTGGAGATTGA  >  CP009273/332453‑332742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑