Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,070,543 G→T 92.6% D57Y (GAT→TAT)  dtd → D‑tyr‑tRNA(Tyr) deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,070,5430GT92.6% 601.9 / 39.4 216D57Y (GAT→TAT) dtdD‑tyr‑tRNA(Tyr) deacylase
Reads supporting (aligned to +/- strand):  ref base G (8/8);  new base T (90/109);  total (99/117)
Fisher's exact test for biased strand distribution p-value = 7.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 216 total aligned reads displayed.

ACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGT  >  CP009273/4070399‑4070687
                                                                                                                                                |                                                                                                                                                
   cGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCg                                                                                                                                            >  2:3312561/1‑148 (MQ=255)
      gCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAg                                                                                                                                         >  2:2603612/1‑148 (MQ=255)
      gCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTGTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAg                                                                                                                                         >  2:3226425/1‑148 (MQ=255)
       ccAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAgg                                                                                                                                        <  2:1958581/148‑1 (MQ=255)
       ccAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAgg                                                                                                                                        <  2:2522685/148‑1 (MQ=255)
       ccAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAgg                                                                                                                                        <  2:1748300/148‑1 (MQ=255)
         aGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCa                                                                                                                                      >  1:2482571/1‑148 (MQ=255)
          gCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCac                                                                                                                                     >  1:2708694/1‑147 (MQ=255)
          gCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCaa                                                                                                                                     >  2:1163161/1‑148 (MQ=255)
           cGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAg                                                                                                                                    >  1:1215599/1‑148 (MQ=255)
            gTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGa                                                                                                                                   <  2:2373627/148‑1 (MQ=255)
             tCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGAt                                                                                                                                  >  1:624667/1‑148 (MQ=255)
             tCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGAt                                                                                                                                  >  1:355507/1‑148 (MQ=255)
              cACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATg                                                                                                                                 >  1:1465006/1‑148 (MQ=255)
                 cGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAAt                                                                                                                              <  2:2848537/148‑1 (MQ=255)
                    ggagggagAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAAtctc                                                                                                                           >  1:3117809/1‑148 (MQ=255)
                     gagggagAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCa                                                                                                                          >  2:827011/1‑148 (MQ=255)
                      agggagAAGTGACGGGCTAAATTGGCGGGGTAATTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCaa                                                                                                                         <  2:1729615/148‑1 (MQ=255)
                      agggagAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCaa                                                                                                                         <  1:48821/148‑1 (MQ=255)
                          agaAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTg                                                                                                                     <  1:1285139/148‑1 (MQ=255)
                             aGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCaa                                                                                                                  <  2:1652178/148‑1 (MQ=255)
                             aGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCaa                                                                                                                  <  2:859737/148‑1 (MQ=255)
                                gACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAg                                                                                                               <  1:2006908/148‑1 (MQ=255)
                                gACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAg                                                                                                               <  1:1962385/148‑1 (MQ=255)
                                 aCGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAgg                                                                                                              >  1:2197167/1‑148 (MQ=255)
                                      cGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGACCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAggcgggc                                                                                                         <  2:2266377/148‑1 (MQ=255)
                                       gAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAggcgggcg                                                                                                        <  1:832520/148‑1 (MQ=255)
                                         aaTTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGggcggc                                                                                                      <  2:1100911/148‑1 (MQ=255)
                                          aTTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCa                                                                                                     >  1:2037777/1‑148 (MQ=255)
                                          aTTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCa                                                                                                     <  1:829004/148‑1 (MQ=255)
                                           ttGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAg                                                                                                    >  2:2738498/1‑148 (MQ=255)
                                           ttGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAg                                                                                                    >  2:1966025/1‑148 (MQ=255)
                                            tGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACTACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAgt                                                                                                   >  2:1265028/1‑148 (MQ=255)
                                            tGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAgt                                                                                                   >  1:3125114/1‑148 (MQ=255)
                                             ggCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAgtg                                                                                                  <  2:602807/148‑1 (MQ=255)
                                             ggCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAgtg                                                                                                  <  1:827011/148‑1 (MQ=255)
                                              gcgcGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAgtgt                                                                                                 <  2:1631307/148‑1 (MQ=255)
                                               cgcgGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGtgtg                                                                                                <  1:839062/148‑1 (MQ=255)
                                                gcgGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGc                                                                                               <  1:1096508/148‑1 (MQ=255)
                                                  gggACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtg                                                                                             <  2:2550465/148‑1 (MQ=255)
                                                    gACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtggt                                                                                           >  2:2138041/1‑148 (MQ=255)
                                                      cTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtggtgg                                                                                         <  1:872011/148‑1 (MQ=255)
                                                      cTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtggtgg                                                                                         <  1:2402857/148‑1 (MQ=255)
                                                          tGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTc                                                                                     <  2:2334766/148‑1 (MQ=255)
                                                           ggTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTcc                                                                                    <  2:2711281/148‑1 (MQ=255)
                                                           ggTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTcc                                                                                    <  2:1461708/148‑1 (MQ=255)
                                                           ggTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGAAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTcc                                                                                    <  1:145090/148‑1 (MQ=255)
                                                            gtgtTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTccc                                                                                   <  1:1543921/148‑1 (MQ=255)
                                                            gtgtTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTccc                                                                                   <  1:1108849/148‑1 (MQ=255)
                                                                tATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGtt                                                                               >  2:907956/1‑148 (MQ=255)
                                                                tATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGtt                                                                               >  1:1300790/1‑148 (MQ=255)
                                                                  ttGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTa                                                                             >  2:849792/1‑148 (MQ=255)
                                                                  ttGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTa                                                                             >  2:3153148/1‑148 (MQ=255)
                                                                    gggTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTAcc                                                                           <  2:2299274/148‑1 (MQ=255)
                                                                     ggTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTAccc                                                                          >  2:714405/1‑148 (MQ=255)
                                                                     ggTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTAccc                                                                          >  2:2487662/1‑148 (MQ=255)
                                                                        gtCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGANGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTcg                                                                       <  1:1677082/148‑1 (MQ=255)
                                                                             aaaGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCa                                                                  >  2:1317410/1‑148 (MQ=255)
                                                                              aaGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAg                                                                 <  2:3125114/148‑1 (MQ=255)
                                                                                ggATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGAt                                                               >  2:2063999/1‑148 (MQ=255)
                                                                                 gATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATa                                                              >  1:1132413/1‑148 (MQ=255)
                                                                                  aTGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCtggtg                                                                                          >  2:1241207/1‑119 (MQ=255)
                                                                                  aTGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATAc                                                             >  1:1528152/1‑148 (MQ=255)
                                                                                    gacgacGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCg                                                           >  2:1686919/1‑148 (MQ=255)
                                                                                    gacgacGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCg                                                           >  2:1442793/1‑148 (MQ=255)
                                                                                     acgacgAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGa                                                          <  1:3194082/148‑1 (MQ=255)
                                                                                      cgacgaACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGaa                                                         <  2:1465006/148‑1 (MQ=255)
                                                                                          gaACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACggg                                                     <  1:1632908/148‑1 (MQ=255)
                                                                                            aCAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACggtgg                                                   <  2:1572562/148‑1 (MQ=255)
                                                                                             cAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGa                                                  <  1:3063622/148‑1 (MQ=255)
                                                                                               gAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCAGCTTTAGCTATGCCTAAGGCAAGATGAATCTCAACGTGCAACATGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGAGGGATg                                                >  1:2515745/1‑148 (MQ=255)
                                                                                                aaaGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATgc                                               >  2:1680454/1‑148 (MQ=255)
                                                                                                aaaGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATgc                                               >  2:2821452/1‑148 (MQ=255)
                                                                                                  aGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATgcgc                                             >  2:472227/1‑148 (MQ=255)
                                                                                                   gCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGcc                                            <  2:3147297/148‑1 (MQ=255)
                                                                                                          gTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGttt                                     <  1:988527/148‑1 (MQ=255)
                                                                                                          gTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGttt                                     <  2:1950776/148‑1 (MQ=255)
                                                                                                          gTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGttt                                     <  1:1247805/148‑1 (MQ=255)
                                                                                                          gTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGttt                                     <  1:192369/148‑1 (MQ=255)
                                                                                                          gTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGttt                                     >  2:2777615/1‑148 (MQ=255)
                                                                                                          gTCTGTGCGAGCCTGTGCTCGGCTACCCCATCTTTAGCAATGCCGAACGCAACATGAATCTCAACGTGCAACACGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCACGCCGCAGATACCCAACGGGGGATGCGCCCAAGttt                                     >  2:3050042/1‑148 (MQ=255)
                                                                                                            cTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTtct                                   >  2:2701428/1‑148 (MQ=255)
                                                                                                            cTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTtct                                   <  1:388321/148‑1 (MQ=255)
                                                                                                                gCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCaa                               <  1:1702247/148‑1 (MQ=255)
                                                                                                                   aGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAgg                            <  2:1699335/148‑1 (MQ=255)
                                                                                                                   aGCGTGTGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAgg                            <  1:2300148/148‑1 (MQ=255)
                                                                                                                      gtgtGCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGc                         <  2:14359/148‑1 (MQ=255)
                                                                                                                        gtgCTCGGCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCAt                       >  1:3024269/1‑148 (MQ=255)
                                                                                                                               gCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGa                <  1:2603612/148‑1 (MQ=255)
                                                                                                                               gCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGa                >  2:15057/1‑148 (MQ=255)
                                                                                                                               gCTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGa                >  1:2769797/1‑148 (MQ=255)
                                                                                                                                cTACCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGAt               >  2:2929599/1‑148 (MQ=255)
                                                                                                                                  aCCGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATcg             >  2:1779746/1‑148 (MQ=255)
                                                                                                                                   ccGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATcgc            >  2:1448461/1‑148 (MQ=255)
                                                                                                                                    cGCATCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATcgcg           >  1:46773/1‑148 (MQ=255)
                                                                                                                                       aTCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCag        >  1:290953/1‑148 (MQ=255)
                                                                                                                                       aTCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCag        >  1:3300171/1‑148 (MQ=255)
                                                                                                                                        tCTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCaga       >  2:3017176/1‑148 (MQ=255)
                                                                                                                                         cTTTAGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCagag      >  1:2331984/1‑148 (MQ=255)
                                                                                                                                             aGCTATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGt  <  1:714405/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACCCGTGCCAGCGTCACCGTGGAGGGAGAAGTGACGGGCGAAATTGGCGCGGGACTTTTGGTGTTATTGGGTGTCGAAAAGGATGACGACGAACAGAAAGCAAACCGTCTGTGCGAGCGTGTGCTCGGCTACCGCATCTTTAGCGATGCCGAAGGCAAGATGAATCTCAACGTGCAACAGGCGGGCGGCAGTGTGCTGGTGGTTTCCCAGTTTACCCTCGCCGCAGATACCGAACGGGGGATGCGCCCAAGTTTCTCCAAAGGTGCATCACCGGATCGCGCAGAGGCGT  >  CP009273/4070399‑4070687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑