Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,124,384 C→A 88.4% H208N (CAT→AAT)  katG → catalase‑peroxidase HPI, heme b‑containing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,124,3840CA88.4% 362.9 / 46.8 154H208N (CAT→AAT) katGcatalase‑peroxidase HPI, heme b‑containing
Reads supporting (aligned to +/- strand):  ref base C (10/8);  new base A (84/52);  total (94/60)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 154 total aligned reads displayed.

GGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGAACG  >  CP009273/4124239‑4124528
                                                                                                                                                 |                                                                                                                                                
ggCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAAt                                                                                                                                                >  2:2963617/1‑148 (MQ=255)
   cGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCg                                                                                                                                             >  2:566236/1‑148 (MQ=255)
     aCCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGa                                                                                                                                           >  1:513561/1‑148 (MQ=255)
     aCCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGa                                                                                                                                           >  2:3073671/1‑148 (MQ=255)
      ccTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGaa                                                                                                                                          <  1:778037/148‑1 (MQ=255)
         gTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAgcg                                                                                                                                       >  2:1990127/1‑148 (MQ=255)
          tttATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAgcgc                                                                                                                                      >  1:2014403/1‑148 (MQ=255)
           ttATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCt                                                                                                                                     >  1:1127358/1‑148 (MQ=255)
           ttATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCt                                                                                                                                     >  1:1128226/1‑148 (MQ=255)
             aTCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTgg                                                                                                                                   <  2:2084943/148‑1 (MQ=255)
             aTCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTgg                                                                                                                                   >  2:500326/1‑148 (MQ=255)
             aTCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTgg                                                                                                                                   <  2:420008/148‑1 (MQ=255)
               ccTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCg                                                                                                                                 >  2:1862883/1‑148 (MQ=255)
               ccTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCg                                                                                                                                 >  2:1862223/1‑148 (MQ=255)
                  cgcgGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGaaa                                                                                                                              <  1:2482196/148‑1 (MQ=255)
                    cgGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGc                                                                                                                            <  2:2024854/148‑1 (MQ=255)
                        tAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCg                                                                                                                        <  1:2941693/148‑1 (MQ=255)
                        tAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCg                                                                                                                        <  1:2477346/148‑1 (MQ=255)
                          aCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCt                                                                                                                      <  2:2959396/148‑1 (MQ=255)
                          aCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCt                                                                                                                      <  2:3175306/148‑1 (MQ=255)
                                   tAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGACGCGCTGGCGAAAGCACCGCTGGGTGCacc                                                                                                             >  2:1489082/1‑146 (MQ=255)
                                   tAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAAc                                                                                                             >  1:2537958/1‑148 (MQ=255)
                                     gAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCg                                                                                                           >  2:2298874/1‑148 (MQ=255)
                                     gAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCg                                                                                                           >  1:1879444/1‑148 (MQ=255)
                                      acaaCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTACCTGGGGTGATGCAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCga                                                                                                          <  2:3268387/146‑1 (MQ=255)
                                      aaaaCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCga                                                                                                          >  1:3235701/1‑148 (MQ=255)
                                      aaaaCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCga                                                                                                          >  1:1596735/1‑148 (MQ=255)
                                      aaaaCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCga                                                                                                          >  2:957032/1‑148 (MQ=255)
                                       aaaCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGACGCCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCgag                                                                                                         <  2:1698113/148‑1 (MQ=255)
                                       aaaCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCgag                                                                                                         <  1:63968/148‑1 (MQ=255)
                                         aCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGAt                                                                                                       <  1:2708223/148‑1 (MQ=255)
                                             cGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGt                                                                                                   <  2:513561/148‑1 (MQ=255)
                                              ggCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTc                                                                                                  >  2:2822223/1‑148 (MQ=255)
                                                 ttCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTACTCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGa                                                                                               >  2:1123098/1‑148 (MQ=255)
                                                 ttCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGa                                                                                               >  2:1124230/1‑148 (MQ=255)
                                                    cGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGAttt                                                                                            <  1:1217824/148‑1 (MQ=255)
                                                      tACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTAc                                                                                          >  2:1883428/1‑148 (MQ=255)
                                                      tACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCAGGCTGACTCACCGTAATCCCGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTAc                                                                                          >  1:847467/1‑148 (MQ=255)
                                                      tACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACGGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCGGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTAc                                                                                          <  2:1752767/148‑1 (MQ=255)
                                                       aCCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACg                                                                                         >  2:3265655/1‑148 (MQ=255)
                                                       aCCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACg                                                                                         <  2:2370191/148‑1 (MQ=255)
                                                         cTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGtt                                                                                       >  1:2020845/1‑148 (MQ=255)
                                                          ttCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTa                                                                                      <  1:3029154/148‑1 (MQ=255)
                                                          ttCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTa                                                                                      <  1:1480758/148‑1 (MQ=255)
                                                          ttCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTa                                                                                      <  1:699440/148‑1 (MQ=255)
                                                           tCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTaa                                                                                     >  2:91535/1‑148 (MQ=255)
                                                            cGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAAc                                                                                    >  2:3012939/1‑148 (MQ=255)
                                                               ttttGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCg                                                                                 >  1:347492/1‑148 (MQ=255)
                                                               ttttGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCg                                                                                 >  1:346646/1‑148 (MQ=255)
                                                               ttttGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCg                                                                                 >  1:2775511/1‑148 (MQ=255)
                                                                tttGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCgg                                                                                >  1:2490935/1‑148 (MQ=255)
                                                                 ttGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGa                                                                               <  2:1083494/148‑1 (MQ=255)
                                                                    gTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTACTCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAgg                                                                            >  2:2818101/1‑148 (MQ=255)
                                                                    gTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAgg                                                                            <  1:2396545/148‑1 (MQ=255)
                                                                      gCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGcc                                                                          >  1:2259213/1‑148 (MQ=255)
                                                                         ggTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCgg                                                                       <  1:2911844/148‑1 (MQ=255)
                                                                          gTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGa                                                                      <  1:2516639/148‑1 (MQ=255)
                                                                          gTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGa                                                                      <  2:2707338/148‑1 (MQ=255)
                                                                           tCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGAt                                                                     >  2:1504593/1‑148 (MQ=255)
                                                                              tGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATcac                                                                  >  2:714938/1‑148 (MQ=255)
                                                                              tGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATcac                                                                  >  2:714748/1‑148 (MQ=255)
                                                                                aaGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAg                                                                <  1:1917257/148‑1 (MQ=255)
                                                                                aaGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAg                                                                <  2:1440727/148‑1 (MQ=255)
                                                                                 aGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgc                                                               >  1:1661035/1‑148 (MQ=255)
                                                                                  gACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgcg                                                              <  2:1421307/148‑1 (MQ=255)
                                                                                          ggAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCg                                                      >  2:564161/1‑148 (MQ=255)
                                                                                           gAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGTAAGGCCCGGATCACAGCGGCGAACCGc                                                     >  1:2950625/1‑148 (MQ=255)
                                                                                           gAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGc                                                     <  1:1023867/148‑1 (MQ=255)
                                                                                            aaCCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCt                                                    <  2:1958289/148‑1 (MQ=255)
                                                                                              ccGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCttt                                                  <  2:1279542/148‑1 (MQ=255)
                                                                                                  aTCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTACTCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGc                                              >  2:2292429/1‑148 (MQ=255)
                                                                                                   tCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCg                                             >  1:1752768/1‑148 (MQ=255)
                                                                                                    cTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCgg                                            >  1:3247529/1‑148 (MQ=255)
                                                                                                         tGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGgcagca                                       >  1:2879095/1‑148 (MQ=255)
                                                                                                         tGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGgcagca                                       >  1:2721839/1‑148 (MQ=255)
                                                                                                          gTTAACTGGGGTGATGAAAAAGCCTTGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGcagcag                                      >  1:2291613/1‑148 (MQ=255)
                                                                                                          gTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGcagcag                                      >  2:1249873/1‑148 (MQ=255)
                                                                                                              aCTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTAt                                  <  1:3052043/148‑1 (MQ=255)
                                                                                                               cTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATc                                 <  1:385178/148‑1 (MQ=255)
                                                                                                               cTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATc                                 >  2:3012228/1‑148 (MQ=255)
                                                                                                                  gggTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCcgc                              >  1:3277141/1‑148 (MQ=255)
                                                                                                                   ggTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCcgcg                             <  1:1345499/148‑1 (MQ=255)
                                                                                                                   ggTGATGAAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCcgcg                             >  2:1038810/1‑148 (MQ=255)
                                                                                                                         gaAAAAGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCt                       <  1:2791445/148‑1 (MQ=255)
                                                                                                                           aaaaGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTc                     >  2:2572148/1‑148 (MQ=255)
                                                                                                                              aGCCTGGCTGACTCACCGTCATCCGGAATCGCTGGCGAAAGCACAGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGc                  >  1:909193/1‑148 (MQ=255)
                                                                                                                              aGCCTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGc                  >  2:1886154/1‑148 (MQ=255)
                                                                                                                                ccTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCaa                >  2:718158/1‑148 (MQ=255)
                                                                                                                                 cTGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAAc               >  1:1123155/1‑148 (MQ=255)
                                                                                                                                  tGGCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACa              >  2:2623131/1‑148 (MQ=255)
                                                                                                                                   ggCTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACAt             >  1:1340923/1‑148 (MQ=255)
                                                                                                                                     cTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATgg           >  2:1258029/1‑148 (MQ=255)
                                                                                                                                     cTGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATgg           >  2:601135/1‑148 (MQ=255)
                                                                                                                                      tGACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATggg          <  1:2768744/148‑1 (MQ=255)
                                                                                                                                       gACTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGc         <  2:1404727/148‑1 (MQ=255)
                                                                                                                                        aCTCACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCa        <  1:1665263/148‑1 (MQ=255)
                                                                                                                                           cACCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGa     <  2:1616198/148‑1 (MQ=255)
                                                                                                                                            aCCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGaa    >  2:897645/1‑148 (MQ=255)
                                                                                                                                            aCCGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGaa    >  2:2378965/1‑148 (MQ=255)
                                                                                                                                              cGTAATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGAacg  >  1:1082478/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGGCGAACCGCTTTCTGCGGCAGCAGCTATCCGCGCGACCTTCGGCAACATGGGCATGAACG  >  CP009273/4124239‑4124528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑