Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,207,552 G→T 93.8% M172I (ATG→ATT)  aceA → isocitrate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,207,5520GT93.8% 502.3 / 24.8 177M172I (ATG→ATT) aceAisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base G (5/6);  new base T (78/88);  total (83/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 179 total aligned reads displayed.

ACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG  >  CP009273/4207410‑4207697
                                                                                                                                              |                                                                                                                                                 
acaacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGc                                                                                                                                              >  2:1536369/1‑148 (MQ=255)
acaacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGc                                                                                                                                              <  1:1852490/148‑1 (MQ=255)
 caacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCg                                                                                                                                             >  2:2550742/1‑148 (MQ=255)
  aacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGcta                                                                                                                                            <  1:3112542/148‑3 (MQ=255)
   acacCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCgat                                                                                                                                           >  2:1795459/1‑148 (MQ=255)
    cacCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCgatg                                                                                                                                          <  2:2048156/148‑1 (MQ=255)
     acCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCgatga                                                                                                                                         <  2:1203093/148‑1 (MQ=255)
     acCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCgatga                                                                                                                                         <  2:1203053/148‑1 (MQ=255)
     acCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCtatga                                                                                                                                         >  1:482807/1‑148 (MQ=255)
      ccTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCgatgat                                                                                                                                        <  2:2447899/148‑1 (MQ=255)
         tCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGa                                                                                                                                     <  2:3153272/148‑1 (MQ=255)
         tCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGa                                                                                                                                     >  1:3298157/1‑148 (MQ=255)
         tCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGa                                                                                                                                     <  2:3153266/148‑1 (MQ=255)
            gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGc                                                                                                                                  <  1:1666835/148‑1 (MQ=255)
            gtcgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGc                                                                                                                                  <  2:2960277/148‑1 (MQ=255)
              cgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCg                                                                                                                                >  1:1812124/1‑148 (MQ=255)
              cgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCg                                                                                                                                >  1:1922952/1‑148 (MQ=255)
              cgtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCg                                                                                                                                >  2:1854422/1‑148 (MQ=255)
               gtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCgg                                                                                                                               <  2:654651/148‑1 (MQ=255)
                    gATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAg                                                                                                                          >  2:2967788/1‑148 (MQ=255)
                     aTCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGc                                                                                                                         >  1:1432472/1‑148 (MQ=255)
                     aTCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGc                                                                                                                         >  1:2863079/1‑148 (MQ=255)
                      tCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCg                                                                                                                        >  1:3170113/1‑148 (MQ=255)
                      tCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCg                                                                                                                        >  1:3014614/1‑148 (MQ=255)
                      tCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCg                                                                                                                        >  1:3014626/1‑148 (MQ=255)
                       cAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCgg                                                                                                                       >  2:2531531/1‑148 (MQ=255)
                       cAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCgg                                                                                                                       >  1:1293314/1‑148 (MQ=255)
                         gATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCa                                                                                                                     >  1:2749564/1‑148 (MQ=255)
                              aaTGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCa                                                                                                                <  2:640751/148‑1 (MQ=255)
                              aaTGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCa                                                                                                                >  2:1646645/1‑148 (MQ=255)
                               aTGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCAc                                                                                                               <  2:2952267/148‑1 (MQ=255)
                                  gTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTc                                                                                                            >  2:2309525/1‑148 (MQ=255)
                                  gTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTc                                                                                                            >  2:2309362/1‑148 (MQ=255)
                                  gTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTc                                                                                                            >  2:2311470/1‑148 (MQ=255)
                                   tCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCg                                                                                                           >  2:1435406/1‑148 (MQ=255)
                                    ccGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGa                                                                                                          <  1:2275741/148‑1 (MQ=255)
                                    ccGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGa                                                                                                          <  1:2275687/148‑1 (MQ=255)
                                        gggCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGAt                                                                                                      <  2:1897523/148‑1 (MQ=255)
                                         ggCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATc                                                                                                     <  2:1694467/148‑1 (MQ=255)
                                            aTTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGc                                                                                                  <  1:2920872/148‑1 (MQ=255)
                                             ttGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCt                                                                                                 <  2:988366/148‑1 (MQ=255)
                                               gAGGCGGGCGATGAGCGCTATGTCGATTACTTCCAGCCGATCGTTGCCGTTGCGGTAGCCGGTTTTGGGGGTGTCCTGAATGCCTTTGTTCTGATTCAAGCGATGAATGAAGCCGCTGCAGCGGCAGTTCACTTCGAAGATCAGCTgg                                                                                               <  2:1116160/148‑1 (MQ=255)
                                                aGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGc                                                                                              >  1:101119/1‑148 (MQ=255)
                                                     ggCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAg                                                                                         <  2:2804559/148‑1 (MQ=255)
                                                      gCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGt                                                                                        >  1:3285030/1‑148 (MQ=255)
                                                       cGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTg                                                                                       <  2:2346750/148‑1 (MQ=255)
                                                       cGATCCGCGCTATGTCGATTACGTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGTCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTg                                                                                       <  2:2659587/148‑1 (MQ=255)
                                                        gATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTga                                                                                      <  2:1305143/148‑1 (MQ=255)
                                                         aTCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaa                                                                                     >  1:383568/1‑148 (MQ=255)
                                                         aTCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaa                                                                                     >  2:2508024/1‑148 (MQ=255)
                                                          tCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaag                                                                                    >  2:2188417/1‑148 (MQ=255)
                                                           ccGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaaga                                                                                   >  2:224841/1‑148 (MQ=255)
                                                           ccGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaaga                                                                                   >  2:224963/1‑148 (MQ=255)
                                                            cgcgCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaagaa                                                                                  <  1:1771538/148‑1 (MQ=255)
                                                             gcgcTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGaaa                                                                                 <  2:2980152/148‑1 (MQ=255)
                                                             gcgcTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGaaa                                                                                 <  1:2816823/148‑1 (MQ=255)
                                                                cTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGc                                                                              <  2:1400381/148‑1 (MQ=255)
                                                                cTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGc                                                                              >  1:481926/1‑148 (MQ=255)
                                                                   tGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGt                                                                           >  2:1485514/1‑148 (MQ=255)
                                                                   tGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGt                                                                           >  2:1658858/1‑148 (MQ=255)
                                                                     tCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTca                                                                         >  2:2592320/1‑148 (MQ=255)
                                                                      cGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTcac                                                                        >  1:1908558/1‑148 (MQ=255)
                                                                             ttCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGggcg                                                                 >  1:2409879/1‑148 (MQ=255)
                                                                              tCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGggcgg                                                                <  2:1683240/148‑1 (MQ=255)
                                                                                 tGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCaa                                                             <  1:1658858/148‑1 (MQ=255)
                                                                                 tGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCaa                                                             <  1:1485514/148‑1 (MQ=255)
                                                                                   ccGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAg                                                           >  2:2614247/1‑148 (MQ=255)
                                                                                     gATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGtt                                                         <  1:1600790/148‑1 (MQ=255)
                                                                                      aTCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGttt                                                        >  2:1693835/1‑148 (MQ=255)
                                                                                      aTCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGttt                                                        >  2:1628637/1‑148 (MQ=255)
                                                                                        cGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTa                                                      >  2:72122/1‑148 (MQ=255)
                                                                                          ttGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGt                                                    <  2:1776624/148‑1 (MQ=255)
                                                                                          ttGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGt                                                    >  2:3077994/1‑148 (MQ=255)
                                                                                                aTGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAAc                                              >  2:2351440/1‑148 (MQ=255)
                                                                                                  gCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTc                                            <  1:2842548/148‑1 (MQ=255)
                                                                                                  gCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTc                                            <  1:1795113/148‑1 (MQ=255)
                                                                                                   cGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCa                                           <  1:2682212/148‑1 (MQ=255)
                                                                                                     gAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATTGGCGGCAAAGTTTTAGTGCCAACTCAgg                                         <  1:3135487/148‑1 (MQ=255)
                                                                                                      aaGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAGAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGa                                        <  1:840820/148‑1 (MQ=255)
                                                                                                         ccGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGc                                     <  2:1452727/148‑1 (MQ=255)
                                                                                                         ccGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGc                                     >  1:1383276/1‑148 (MQ=255)
                                                                                                         ccGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGc                                     <  1:2896795/148‑1 (MQ=255)
                                                                                                         ccGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGc                                     >  1:1383367/1‑148 (MQ=255)
                                                                                                            gTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTAt                                  <  2:1388146/148‑1 (MQ=255)
                                                                                                             ttttGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTAtt                                 <  2:831905/148‑1 (MQ=255)
                                                                                                             ttttGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTAtt                                 >  1:1000714/1‑148 (MQ=255)
                                                                                                                   cGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGaaa                           <  2:388607/148‑1 (MQ=255)
                                                                                                                   cGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGaaa                           <  2:1451591/148‑1 (MQ=255)
                                                                                                                    ggTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAAc                          <  1:2971043/148‑1 (MQ=255)
                                                                                                                      tgtCCTGAATGCCTTTGAACTCATTAAAGCGATGATTGAATCCGGTGCAGCGGCAGTTAACTTCGAATATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGTAAGCTATTCAGAAACTg                        >  1:2514168/1‑148 (MQ=255)
                                                                                                                       gtCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTgg                       <  1:224842/148‑1 (MQ=255)
                                                                                                                           tGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTcgc                   >  1:591163/1‑148 (MQ=255)
                                                                                                                            gAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTcgcg                  >  1:1069426/1‑148 (MQ=255)
                                                                                                                             aaTGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCgcgg                 <  1:2508024/148‑1 (MQ=255)
                                                                                                                                  cTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGt            <  2:3298216/148‑1 (MQ=255)
                                                                                                                                   tttGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTc           >  2:694001/1‑148 (MQ=255)
                                                                                                                                     tGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTg         <  2:1000714/148‑1 (MQ=255)
                                                                                                                                         cTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAg     <  2:3014626/148‑1 (MQ=255)
                                                                                                                                          tgattaAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGc    >  1:2556719/1‑148 (MQ=255)
                                                                                                                                            atgaAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCtg  >  1:201527/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
ACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTG  >  CP009273/4207410‑4207697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑