Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,219,790 A→T 50.0% L94Q (CTG→CAG)  pepE ← (alpha)‑aspartyl dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,219,7900AT50.0% ‑6.5 / inf 194L94Q (CTG→CAG) pepE(alpha)‑aspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (43/54);  new base T (49/48);  total (92/102)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

TGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCA  >  CP009273/4219646‑4219935
                                                                                                                                                |                                                                                                                                                 
tGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCa                                                                                                                                                >  2:425668/1‑148 (MQ=255)
 gCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCaa                                                                                                                                               <  2:2867304/148‑1 (MQ=255)
     aTCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTgg                                                                                                                                           >  1:1923534/1‑148 (MQ=255)
       cGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGaa                                                                                                                                         <  1:2331559/148‑1 (MQ=255)
         aCAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAg                                                                                                                                       >  2:2426578/1‑148 (MQ=255)
         aCAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAg                                                                                                                                       >  1:1072818/1‑148 (MQ=255)
         aCAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAg                                                                                                                                       <  1:515535/148‑1 (MQ=255)
         aCAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAg                                                                                                                                       <  2:1351398/148‑1 (MQ=255)
          cAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGt                                                                                                                                      >  2:1570576/1‑148 (MQ=255)
           aaTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTa                                                                                                                                     <  1:1067107/148‑1 (MQ=255)
           aaTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTa                                                                                                                                     <  2:1917338/148‑1 (MQ=255)
           aaTCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTa                                                                                                                                     <  1:574190/148‑1 (MQ=255)
              cGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTc                                                                                                                                  <  2:1281545/148‑1 (MQ=255)
                 cATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccg                                                                                                                               >  1:1008517/1‑148 (MQ=255)
                 cATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccg                                                                                                                               >  1:954290/1‑148 (MQ=255)
                 cATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccg                                                                                                                               >  1:835672/1‑148 (MQ=255)
                 cATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccg                                                                                                                               >  2:2309587/1‑148 (MQ=255)
                 cATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccg                                                                                                                               >  2:651102/1‑148 (MQ=255)
                 cATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccg                                                                                                                               <  1:323016/148‑1 (MQ=255)
                   tatCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCccgcc                                                                                                                             >  2:2034622/1‑148 (MQ=255)
                    atCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccg                                                                                                                            >  1:1562510/1‑148 (MQ=255)
                     tCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgc                                                                                                                           >  1:1015982/1‑148 (MQ=255)
                          ggtggtACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACa                                                                                                                      <  1:1910873/148‑1 (MQ=255)
                          ggtggtACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACa                                                                                                                      <  2:1792688/148‑1 (MQ=255)
                            tggtACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAAt                                                                                                                    >  1:276398/1‑148 (MQ=255)
                                 cGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGa                                                                                                               <  1:3292569/148‑1 (MQ=255)
                                  gAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGAt                                                                                                              <  1:256075/148‑1 (MQ=255)
                                   aaTAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGAtc                                                                                                             >  2:765874/1‑148 (MQ=255)
                                      aGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCa                                                                                                          <  1:941896/148‑1 (MQ=255)
                                        ttGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGc                                                                                                        <  1:2111659/148‑1 (MQ=255)
                                         tGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCa                                                                                                       >  1:704439/1‑148 (MQ=255)
                                         tGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCa                                                                                                       <  1:1120904/148‑1 (MQ=255)
                                           ggCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCAtt                                                                                                     >  2:1086287/1‑148 (MQ=255)
                                              aagcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTc                                                                                                  >  2:1523488/1‑148 (MQ=255)
                                               agcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCa                                                                                                 <  1:1887384/148‑1 (MQ=255)
                                                gcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCaa                                                                                                >  2:2463312/1‑148 (MQ=255)
                                                gcaagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCaa                                                                                                >  2:1485159/1‑148 (MQ=255)
                                                 caagGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAAt                                                                                               >  2:1733468/1‑148 (MQ=255)
                                                    ggTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATcgc                                                                                            <  1:1107797/148‑1 (MQ=255)
                                                    ggTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATcgc                                                                                            >  1:439248/1‑148 (MQ=255)
                                                        aGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCCGCATTTTCAATCGCGGCa                                                                                        <  1:809646/148‑1 (MQ=255)
                                                         gcgcCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTATTTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGAAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCATCATTTTCAATCGCGTCaa                                                                                       <  2:1691021/148‑1 (MQ=255)
                                                         gcgcCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCaa                                                                                       <  2:1690858/148‑1 (MQ=255)
                                                          cgcCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAAc                                                                                      >  1:1793503/1‑148 (MQ=255)
                                                              tGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGa                                                                                  <  1:1471945/148‑1 (MQ=255)
                                                              tGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGa                                                                                  <  1:1400429/148‑1 (MQ=255)
                                                              tGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGa                                                                                  <  1:1992071/148‑1 (MQ=255)
                                                              tGCGCTCCAGCCAAGAGACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGa                                                                                  <  2:2556144/148‑1 (MQ=255)
                                                               gcgcTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGAt                                                                                 <  2:70293/148‑1 (MQ=255)
                                                                 gcTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCg                                                                               <  1:1816298/148‑1 (MQ=255)
                                                                 gcTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCg                                                                               <  1:2072846/148‑1 (MQ=255)
                                                                  cTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGa                                                                              >  1:177309/1‑148 (MQ=255)
                                                                    ccAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACa                                                                            <  2:835672/148‑1 (MQ=255)
                                                                    ccAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACa                                                                            <  1:2340757/148‑1 (MQ=255)
                                                                        ccAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGc                                                                        <  1:2658063/148‑1 (MQ=255)
                                                                        ccAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGc                                                                        >  2:2718184/1‑148 (MQ=255)
                                                                         cAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCt                                                                       >  1:3127218/1‑148 (MQ=255)
                                                                           atatACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTAt                                                                     >  1:1862380/1‑148 (MQ=255)
                                                                              taCAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGaa                                                                  <  2:1061352/148‑1 (MQ=255)
                                                                                 agagCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATAc                                                               >  1:560310/1‑148 (MQ=255)
                                                                                  gagCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATAcc                                                              <  2:1562510/148‑1 (MQ=255)
                                                                                  gagCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATAcc                                                              >  1:2929203/1‑148 (MQ=255)
                                                                                   agCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCg                                                             >  2:949080/1‑148 (MQ=255)
                                                                                   agCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCg                                                             >  1:2454351/1‑148 (MQ=255)
                                                                                     cgcCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGt                                                           >  1:2860915/1‑148 (MQ=255)
                                                                                     cgcCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGt                                                           >  2:1414695/1‑148 (MQ=255)
                                                                                     cgcCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGt                                                           >  2:695644/1‑148 (MQ=255)
                                                                                      gcCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTg                                                          <  1:1578073/148‑1 (MQ=255)
                                                                                        cACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGAc                                                        >  2:2600388/1‑148 (MQ=255)
                                                                                         aCGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACa                                                       >  2:875106/1‑148 (MQ=255)
                                                                                          cGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAg                                                      >  1:261551/1‑148 (MQ=255)
                                                                                           gTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGa                                                     <  2:484143/148‑1 (MQ=255)
                                                                                            tttAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGaa                                                    >  1:535549/1‑148 (MQ=255)
                                                                                             ttAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGaaa                                                   >  2:1404564/1‑148 (MQ=255)
                                                                                                 ccACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACAcc                                               <  1:15614/148‑1 (MQ=255)
                                                                                                  cACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACAccc                                              <  2:2854224/148‑1 (MQ=255)
                                                                                                  cACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACAccc                                              <  2:2854228/148‑1 (MQ=255)
                                                                                                  cACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACAccc                                              <  1:1874949/148‑1 (MQ=255)
                                                                                                    cGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAg                                            <  2:2263484/148‑1 (MQ=255)
                                                                                                          tAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGc                                      >  1:961370/1‑148 (MQ=255)
                                                                                                                          cccGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtt                      >  1:2093741/1‑148 (MQ=255)
                                                                                                                          cccGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtt                      <  2:1650514/148‑1 (MQ=255)
                                                                                                                          cccGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtt                      >  2:1024464/1‑148 (MQ=255)
                                                                                                                          cccGCGCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtg                      >  1:1517404/1‑147 (MQ=255)
                                                                                                                            cgcgCTCGCGGCACTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtttt                    >  2:3245583/1‑148 (MQ=255)
                                                                                                                            cgcgCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGtttt                    >  1:1004197/1‑148 (MQ=255)
                                                                                                                               gcTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGc                 >  2:722469/1‑148 (MQ=255)
                                                                                                                                     ggCACTGTTTCTGCAACTGGAAAGTATTCCTGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTa           <  1:2148389/148‑1 (MQ=255)
                                                                                                                                       cACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAat         <  1:1485159/148‑1 (MQ=255)
                                                                                                                                        aCTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatc        <  2:2647761/148‑1 (MQ=255)
                                                                                                                                        aCTGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatc        <  2:2647980/148‑1 (MQ=255)
                                                                                                                                        aCTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatc        <  2:1541639/148‑1 (MQ=255)
                                                                                                                                          tGTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatcat      <  1:117585/148‑1 (MQ=255)
                                                                                                                                           gTTTCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAatcatc     >  2:307751/1‑148 (MQ=255)
                                                                                                                                            tttCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCCGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATcc    <  1:3243576/148‑1 (MQ=255)
                                                                                                                                            tttCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATcc    >  2:1253421/1‑148 (MQ=255)
                                                                                                                                            tttCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATcc    <  2:1727601/148‑1 (MQ=255)
                                                                                                                                             ttCTGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATccc   >  2:584542/1‑148 (MQ=255)
                                                                                                                                             ttCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATccc   >  2:25512/1‑148 (MQ=255)
                                                                                                                                              tCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCa  <  2:962485/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGATCTCAGCATTTTCAATCGCGGCAACGGGATCGACAACGCTATGAATACCGGTGACAGAAACACCCAGCGGAGCGAGAACCGCAGCCGTTTTCGCTGTGTAATCATCCCA  >  CP009273/4219646‑4219935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑