Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,224,513 G→T 11.4% A276A (GCG→GCT)  pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,224,5130GT11.4% 505.6 / 23.9 159A276A (GCG→GCT) pgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (66/73);  new base T (11/9);  total (77/82)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

AAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAA  >  CP009273/4224367‑4224659
                                                                                                                                                  |                                                                                                                                                  
 aaGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAt                                                                                                                                                  <  2:279554/148‑1 (MQ=255)
  aGCGGCAGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAtt                                                                                                                                                 >  1:2624481/1‑148 (MQ=255)
     ggCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGc                                                                                                                                              <  2:1999380/148‑1 (MQ=255)
       cAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCt                                                                                                                                            >  1:390669/1‑148 (MQ=255)
        aGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTg                                                                                                                                           <  2:2866511/148‑1 (MQ=255)
        aGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTg                                                                                                                                           <  2:2661628/148‑1 (MQ=255)
            gatgaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGa                                                                                                                                       >  2:3075234/1‑148 (MQ=255)
             atgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAt                                                                                                                                      >  2:25702/1‑148 (MQ=255)
             atgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAt                                                                                                                                      >  1:1237275/1‑148 (MQ=255)
             atgaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAt                                                                                                                                      <  1:1849824/148‑1 (MQ=255)
             atgaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGAGGCGTTGTGGTGAGCGATTGGGCGGTCgct                                                                                                                                      >  1:1476067/1‑146 (MQ=255)
              tgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAtt                                                                                                                                     <  1:252661/148‑1 (MQ=255)
               gaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTg                                                                                                                                    <  2:644034/148‑1 (MQ=255)
               gaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACGGTTTGTGGTGAGCGATTGGCCAGTCGATTg                                                                                                                                    >  1:2294506/1‑148 (MQ=255)
                      acGTTGCAAAACACTTGGCGGCGCTTTCCACCACGGCCACAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTtctctc                                                                                                                             <  2:341361/148‑1 (MQ=255)
                       ctttGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTcc                                                                                                                            >  1:3026710/3‑148 (MQ=255)
                       cGTTGCAAACCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTcc                                                                                                                            <  2:3199795/148‑1 (MQ=255)
                            cAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCgg                                                                                                                       <  2:472135/148‑1 (MQ=255)
                             aaaaCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGc                                                                                                                      <  2:2178821/148‑1 (MQ=255)
                              aaaCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCt                                                                                                                     <  2:2333867/148‑1 (MQ=255)
                              aaaCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAAAATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTTTGGTCAGCGATTGGCCTGTCGATTGTTCACTCCAAAGGCt                                                                                                                     >  2:1791000/1‑148 (MQ=255)
                               aaCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGACTCTTTGTGGTCAGCGATTGGCCTGTCGATTTTTATATCCAAAGGCtt                                                                                                                    >  1:2567360/1‑148 (MQ=255)
                                 cacTTTGCGGCGCTTTCCACCACTGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTg                                                                                                                  <  2:2865876/148‑1 (MQ=255)
                                 cacTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTTGCCTGTCGATTTTTATCTCCCTCGGCTTTg                                                                                                                  >  2:2822245/1‑148 (MQ=255)
                                  acTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGa                                                                                                                 <  2:285262/148‑1 (MQ=255)
                                   cTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGAt                                                                                                                >  2:2483953/1‑148 (MQ=255)
                                   cTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGAt                                                                                                                >  2:1001977/1‑148 (MQ=255)
                                   cTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCAACGGCTTTGAt                                                                                                                >  2:824948/1‑148 (MQ=255)
                                     ttGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATaa                                                                                                              <  1:2313142/148‑1 (MQ=255)
                                     ttGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTAGATTTTTATATCCATAGGCTTTGATaa                                                                                                              >  1:169399/1‑148 (MQ=255)
                                      tGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAAc                                                                                                             <  1:2959244/148‑1 (MQ=255)
                                          gcgcTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCg                                                                                                         >  2:542960/1‑148 (MQ=255)
                                          gcgcTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCg                                                                                                         >  2:542959/1‑148 (MQ=255)
                                           cgcTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGt                                                                                                        <  1:1652419/148‑1 (MQ=255)
                                           cgcTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGt                                                                                                        >  2:1562970/1‑148 (MQ=255)
                                           cgcTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGt                                                                                                        >  1:3277595/1‑148 (MQ=255)
                                           cgcTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGACTCTTTGTGGTCAGCGATTTGCCTGTCGATTGTTCTCTCCCTCGGCGTTGATAAATTCGt                                                                                                        >  1:437072/1‑148 (MQ=255)
                                              tttCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGa                                                                                                     <  1:2014339/148‑1 (MQ=255)
                                               ttCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGaa                                                                                                    <  1:1746836/148‑1 (MQ=255)
                                               ttCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGaa                                                                                                    <  2:3285307/148‑1 (MQ=255)
                                                  caccaATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTg                                                                                                 <  2:1423778/148‑1 (MQ=255)
                                                    ccaATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCt                                                                                               <  2:1151154/148‑1 (MQ=255)
                                                     caATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGACTCTTTGTGGTCAGCTATTGGCCGGTCGATTGTTATGTCCCAAGGCGGTGATAACTTCGTTGTACTGCtt                                                                                              >  1:2320046/1‑148 (MQ=255)
                                                       aTGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCATCGGCGTTGATAACTTAGTTGAACTGCTTTc                                                                                            >  1:2732233/1‑148 (MQ=255)
                                                       aTGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTTATCTCCATCGGCGTTGATAACTTCGTTTAAATTCTTTc                                                                                            >  1:1079846/1‑148 (MQ=255)
                                                       aTGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTc                                                                                            <  2:2905945/148‑1 (MQ=255)
                                                       aTGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCGTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTc                                                                                            >  1:2731903/1‑148 (MQ=255)
                                                        tGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTcc                                                                                           <  2:2976703/148‑1 (MQ=255)
                                                        tGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTcc                                                                                           >  2:3271928/1‑148 (MQ=255)
                                                        tGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTcc                                                                                           <  2:2976656/148‑1 (MQ=255)
                                                         gCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCCACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCg                                                                                          >  2:1039641/1‑148 (MQ=255)
                                                         gCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCg                                                                                          >  2:398774/1‑148 (MQ=255)
                                                         gCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCGTTCCg                                                                                          >  2:3078192/1‑148 (MQ=255)
                                                         gCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCGTTGATAACTTCGTTGAACTGCTTTCCg                                                                                          >  2:1039285/1‑148 (MQ=255)
                                                              aGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTTGTGAGCGATTGGCCTGTCGATTTTTGTCTCCATCGGGGTTGATAAAATAGTTTAAATTCTTTACGGCGca                                                                                     >  1:3133884/1‑148 (MQ=255)
                                                               gCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGcac                                                                                    <  2:3048009/148‑1 (MQ=255)
                                                                ccGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGcaca                                                                                   >  2:2992372/1‑148 (MQ=255)
                                                                ccGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGcaca                                                                                   <  2:2110026/148‑1 (MQ=255)
                                                                    tGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTTTTCTCTCCATAGGCTTTGATAACATAGTTGAACTGCTTTCAGGCGCACAcgcg                                                                               >  2:3085377/1‑148 (MQ=255)
                                                                    tGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACAcgcg                                                                               <  2:1016977/148‑1 (MQ=255)
                                                                    tGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACAcgcg                                                                               <  2:2546855/148‑1 (MQ=255)
                                                                    tGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACAcgcg                                                                               <  2:892065/148‑1 (MQ=255)
                                                                    tGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAGTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTAGTTTTACTGCTTTACGGCGcaaaaccg                                                                               >  2:3084902/1‑142 (MQ=255)
                                                                       cGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTACAACGGCGTTGATAAATTAGTTTAACTGCTTTACGGCGCCCACGCGaag                                                                            >  1:1049153/1‑146 (MQ=255)
                                                                            ttGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACaa                                                                       <  1:15949/148‑1 (MQ=255)
                                                                              ggTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGc                                                                     <  1:1342076/148‑1 (MQ=255)
                                                                                tATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTTTTGTCTACATCGGCTTTGATAACTTCGTTGTAATGCTTTGCGGCGCACACGCGATGGACAAGCAt                                                                   >  2:1402078/1‑148 (MQ=255)
                                                                                tATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCGTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCAACGGCTTTGATAACATCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCAt                                                                   >  2:1439697/1‑148 (MQ=255)
                                                                                 aTTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCAtt                                                                  >  1:1639476/1‑148 (MQ=255)
                                                                                      tACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGCTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTATcc                                                             >  2:1528646/1‑148 (MQ=255)
                                                                                      tACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGCTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAAATGCTTTACGGCGCACACGCGATGGACAAGCATTTATcc                                                             >  2:1529159/1‑148 (MQ=255)
                                                                                        cTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGCTTGGCCTGTCGATTGTTGTCTACCACGGGGTTGTTAACATAGTTGAAAGGCTTTACGGCGCCCACGCGCGGGACAACCATTTATccac                                                           >  2:570099/1‑148 (MQ=255)
                                                                                           ccAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTTCTTTACGGCGCACACGCGATGGACAAGCATTTCTCcaccac                                                        >  2:3146434/1‑148 (MQ=255)
                                                                                             aaCATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGc                                                      <  2:3026710/148‑1 (MQ=255)
                                                                                             aaCATGGTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTGGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGc                                                      <  2:693006/148‑1 (MQ=255)
                                                                                              aCATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGcc                                                     >  2:1644072/1‑148 (MQ=255)
                                                                                                   ttCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCAGCCg                                                >  2:711537/1‑148 (MQ=255)
                                                                                                    tCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTTGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAAATTAGTTTAACTGCTTTCCGGCGCACACGCGATTGACAAGCATTTAACCACCACCCCAGCCga                                               >  1:2437431/1‑148 (MQ=255)
                                                                                                            tGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAAcc                                       <  1:1292094/148‑1 (MQ=255)
                                                                                                               gACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAAcctgc                                    <  2:2294506/148‑1 (MQ=255)
                                                                                                                    ggTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCCTCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTa                               <  2:3281044/148‑1 (MQ=255)
                                                                                                                    ggTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTa                               <  2:948571/148‑1 (MQ=255)
                                                                                                                    ggTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTa                               <  1:1402079/148‑1 (MQ=255)
                                                                                                                     gTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTAc                              <  2:766015/148‑1 (MQ=255)
                                                                                                                         gcggcCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTActgct                          <  1:25702/148‑1 (MQ=255)
                                                                                                                         gcggcCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTActgct                          <  2:1273342/148‑1 (MQ=255)
                                                                                                                             ccGTTACTCTTTGTGGTCAGCTATTGGCCAGTCGATTGTTCTCTCCATAGGCTTTGATAAATTCGTTGAACAGCTTTCCGGCGCACACCCCATGGACAAGCATTTCTCCACCACCCCAGCCGAGAAAAACCAGCCAGTACAGCTGgcg                      >  1:2970995/1‑148 (MQ=255)
                                                                                                                               gTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGAAAAAATCGTTGAACTGCATTCCGGCGCACACGCGATGGACAAGCAATTCTCCACCACGCCTGCCGAGAAAAAACTGCCTGTACTGCTGGCGCt                    >  1:2299566/1‑148 (MQ=255)
                                                                                                                                 tACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGa                  >  2:1641798/1‑148 (MQ=255)
                                                                                                                                 tACTCTTTGTGGTCAGCGATTGGACTGTAGATTGTTCTCTACATAGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCTCGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGa                  <  2:2604890/148‑1 (MQ=255)
                                                                                                                                   ctctTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAAACTGCCTGTACTGCTGGCGCTGAtt                >  1:1145873/1‑148 (MQ=255)
                                                                                                                                       ttGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCa            >  1:102109/1‑148 (MQ=255)
                                                                                                                                        tgtgGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCAt           <  1:2232120/148‑1 (MQ=255)
                                                                                                                                         gtgGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATc          <  2:1937908/148‑1 (MQ=255)
                                                                                                                                           ggTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTg        <  2:1336886/148‑1 (MQ=255)
                                                                                                                                             tCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGt      <  2:1237153/148‑1 (MQ=255)
                                                                                                                                              cAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTa     <  1:584977/148‑1 (MQ=255)
                                                                                                                                              cAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTa     <  1:2247429/148‑1 (MQ=255)
                                                                                                                                              cAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCGTTTATAACTTCGTTTAACAGCGTTCCCGCGCACACGCGAATGACAAGCAATTCTCCACCACCCCTGCCGCGAAAAACCTGCCAGTACTGCAGGCGCTGATTGGGAACTTGTa     >  1:2504799/1‑148 (MQ=255)
                                                                                                                                                 cGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTacaa  >  1:1904912/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAA  >  CP009273/4224367‑4224659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑